RU2636460C2 - Системы и способы применения зависящего от вращения транскрипционного секвенирования - Google Patents
Системы и способы применения зависящего от вращения транскрипционного секвенирования Download PDFInfo
- Publication number
- RU2636460C2 RU2636460C2 RU2013142965A RU2013142965A RU2636460C2 RU 2636460 C2 RU2636460 C2 RU 2636460C2 RU 2013142965 A RU2013142965 A RU 2013142965A RU 2013142965 A RU2013142965 A RU 2013142965A RU 2636460 C2 RU2636460 C2 RU 2636460C2
- Authority
- RU
- Russia
- Prior art keywords
- nucleic acid
- sequencing
- rna polymerase
- interest
- rotation
- Prior art date
Links
- 238000012163 sequencing technique Methods 0.000 title claims abstract description 221
- 238000000034 method Methods 0.000 title claims abstract description 128
- 230000001419 dependent effect Effects 0.000 title description 59
- 238000013518 transcription Methods 0.000 title description 38
- 230000035897 transcription Effects 0.000 title description 38
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 238
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 233
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 233
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims abstract description 160
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims abstract description 157
- 235000011178 triphosphate Nutrition 0.000 claims abstract description 86
- 239000001226 triphosphate Substances 0.000 claims abstract description 86
- 239000007787 solid Substances 0.000 claims abstract description 85
- -1 nucleotide triphosphates Chemical class 0.000 claims abstract description 83
- 239000002773 nucleotide Substances 0.000 claims abstract description 57
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 57
- 239000000758 substrate Substances 0.000 claims abstract description 35
- 239000002777 nucleoside Substances 0.000 claims description 82
- 230000005291 magnetic effect Effects 0.000 claims description 56
- 238000001514 detection method Methods 0.000 claims description 26
- 230000008859 change Effects 0.000 claims description 24
- 239000011159 matrix material Substances 0.000 claims description 22
- 101710137500 T7 RNA polymerase Proteins 0.000 claims description 13
- 239000012472 biological sample Substances 0.000 claims description 13
- 239000011521 glass Substances 0.000 claims description 10
- 241001515965 unidentified phage Species 0.000 claims description 9
- 241000588724 Escherichia coli Species 0.000 claims description 7
- 230000005684 electric field Effects 0.000 claims description 5
- 108020004513 Bacterial RNA Proteins 0.000 claims description 3
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 claims description 2
- 239000000126 substance Substances 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 9
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 abstract description 5
- 238000004445 quantitative analysis Methods 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 230000002103 transcriptional effect Effects 0.000 description 61
- 239000000872 buffer Substances 0.000 description 49
- 239000008187 granular material Substances 0.000 description 45
- 238000006243 chemical reaction Methods 0.000 description 36
- 239000003153 chemical reaction reagent Substances 0.000 description 25
- 239000000523 sample Substances 0.000 description 25
- 108020004414 DNA Proteins 0.000 description 24
- 239000000203 mixture Substances 0.000 description 22
- 230000027455 binding Effects 0.000 description 21
- 238000002360 preparation method Methods 0.000 description 21
- 239000000047 product Substances 0.000 description 21
- 239000002245 particle Substances 0.000 description 18
- 230000003287 optical effect Effects 0.000 description 17
- 102000004190 Enzymes Human genes 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 16
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 13
- 229940098773 bovine serum albumin Drugs 0.000 description 13
- 239000012634 fragment Substances 0.000 description 13
- 238000012545 processing Methods 0.000 description 13
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 12
- 238000004590 computer program Methods 0.000 description 12
- 238000005516 engineering process Methods 0.000 description 12
- 239000012530 fluid Substances 0.000 description 12
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 10
- 238000003860 storage Methods 0.000 description 10
- 239000011541 reaction mixture Substances 0.000 description 9
- 239000000463 material Substances 0.000 description 8
- 239000004793 Polystyrene Substances 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- 229960002685 biotin Drugs 0.000 description 7
- 239000011616 biotin Substances 0.000 description 7
- 239000000835 fiber Substances 0.000 description 7
- 229920000642 polymer Polymers 0.000 description 7
- 229920002223 polystyrene Polymers 0.000 description 7
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 239000011324 bead Substances 0.000 description 6
- 235000020958 biotin Nutrition 0.000 description 6
- 238000003384 imaging method Methods 0.000 description 6
- 238000002372 labelling Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 108010090804 Streptavidin Proteins 0.000 description 5
- 230000009471 action Effects 0.000 description 5
- 230000001186 cumulative effect Effects 0.000 description 5
- 238000005286 illumination Methods 0.000 description 5
- 238000010348 incorporation Methods 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 244000052769 pathogen Species 0.000 description 5
- 229920001223 polyethylene glycol Polymers 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 230000001360 synchronised effect Effects 0.000 description 5
- 239000011534 wash buffer Substances 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 4
- 102000003960 Ligases Human genes 0.000 description 4
- 108090000364 Ligases Proteins 0.000 description 4
- 239000004820 Pressure-sensitive adhesive Substances 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000012148 binding buffer Substances 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000003776 cleavage reaction Methods 0.000 description 4
- 238000004891 communication Methods 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 4
- 229910052759 nickel Inorganic materials 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 4
- 230000007017 scission Effects 0.000 description 4
- 239000010703 silicon Substances 0.000 description 4
- 229910052710 silicon Inorganic materials 0.000 description 4
- 238000012800 visualization Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000005653 Brownian motion process Effects 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 239000012901 Milli-Q water Substances 0.000 description 3
- 102400000267 Rhomboid-related protein 2, N-terminal fragment Human genes 0.000 description 3
- 101800000645 Rhomboid-related protein 2, N-terminal fragment Proteins 0.000 description 3
- 101800000716 Tumor necrosis factor, membrane form Proteins 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 238000005537 brownian motion Methods 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 238000012165 high-throughput sequencing Methods 0.000 description 3
- 229920001519 homopolymer Polymers 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000000813 microcontact printing Methods 0.000 description 3
- 238000010295 mobile communication Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000001902 propagating effect Effects 0.000 description 3
- 239000013545 self-assembled monolayer Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- VHYFNPMBLIVWCW-UHFFFAOYSA-N 4-Dimethylaminopyridine Chemical compound CN(C)C1=CC=NC=C1 VHYFNPMBLIVWCW-UHFFFAOYSA-N 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 2
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000004422 calculation algorithm Methods 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 229910017052 cobalt Inorganic materials 0.000 description 2
- 239000010941 cobalt Substances 0.000 description 2
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical group NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 239000005350 fused silica glass Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000000976 ink Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 238000002493 microarray Methods 0.000 description 2
- 150000003833 nucleoside derivatives Chemical class 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000013515 script Methods 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- SYFQYGMJENQVQT-ZETCQYMHSA-N (2s)-6-amino-2-[bis(carboxymethyl)amino]hexanoic acid Chemical compound NCCCC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O SYFQYGMJENQVQT-ZETCQYMHSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 229960000549 4-dimethylaminophenol Drugs 0.000 description 1
- 108091006112 ATPases Proteins 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001137853 Crenarchaeota Species 0.000 description 1
- 241000270722 Crocodylidae Species 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 102000003915 DNA Topoisomerases Human genes 0.000 description 1
- 108090000323 DNA Topoisomerases Proteins 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 241000701867 Enterobacteria phage T7 Species 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241001137858 Euryarchaeota Species 0.000 description 1
- 229910005335 FePt Inorganic materials 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 102000013446 GTP Phosphohydrolases Human genes 0.000 description 1
- 108091006109 GTPases Proteins 0.000 description 1
- 241000272496 Galliformes Species 0.000 description 1
- 108091006054 His-tagged proteins Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101001042038 Homo sapiens Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial Proteins 0.000 description 1
- 101000901226 Homo sapiens S-arrestin Proteins 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 102100021311 Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial Human genes 0.000 description 1
- 241000270322 Lepidosauria Species 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 102000003505 Myosin Human genes 0.000 description 1
- 108060008487 Myosin Proteins 0.000 description 1
- 229910017855 NH 4 F Inorganic materials 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 241000270285 Pituophis Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 238000012181 QIAquick gel extraction kit Methods 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 101100273253 Rhizopus niveus RNAP gene Proteins 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 102100022135 S-arrestin Human genes 0.000 description 1
- 101000677220 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 60S ribosomal protein L33-A Proteins 0.000 description 1
- 101000677235 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 60S ribosomal protein L33-B Proteins 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 229910052581 Si3N4 Inorganic materials 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000270666 Testudines Species 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 150000001615 biotins Chemical class 0.000 description 1
- 125000004057 biotinyl group Chemical group [H]N1C(=O)N([H])[C@]2([H])[C@@]([H])(SC([H])([H])[C@]12[H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C(*)=O 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 239000003990 capacitor Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000007931 coated granule Substances 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000005672 electromagnetic field Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000003709 image segmentation Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 229920001427 mPEG Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 238000005329 nanolithography Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 239000006199 nebulizer Substances 0.000 description 1
- 238000005312 nonlinear dynamic Methods 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 229910052763 palladium Inorganic materials 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920005594 polymer fiber Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000003906 pulsed field gel electrophoresis Methods 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000000682 scanning probe acoustic microscopy Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- BPSIOYPQMFLKFR-UHFFFAOYSA-N trimethoxy-[3-(oxiran-2-ylmethoxy)propyl]silane Chemical compound CO[Si](OC)(OC)CCCOCC1CO1 BPSIOYPQMFLKFR-UHFFFAOYSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 244000052613 viral pathogen Species 0.000 description 1
- 238000007794 visualization technique Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Saccharide Compounds (AREA)
- Pyrane Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161463850P | 2011-02-23 | 2011-02-23 | |
| US61/463,850 | 2011-02-23 | ||
| US201161574270P | 2011-07-30 | 2011-07-30 | |
| US61/574,270 | 2011-07-30 | ||
| PCT/US2012/026339 WO2012116191A1 (en) | 2011-02-23 | 2012-02-23 | Rotation-dependent transcriptional sequencing systems and methods of using |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2013142965A RU2013142965A (ru) | 2015-03-27 |
| RU2636460C2 true RU2636460C2 (ru) | 2017-11-23 |
Family
ID=45852700
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2013142965A RU2636460C2 (ru) | 2011-02-23 | 2012-02-23 | Системы и способы применения зависящего от вращения транскрипционного секвенирования |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US8574840B2 (enExample) |
| EP (1) | EP2678444B1 (enExample) |
| JP (1) | JP6018092B2 (enExample) |
| KR (1) | KR101924665B1 (enExample) |
| CN (1) | CN103534357B (enExample) |
| AU (1) | AU2012220546B2 (enExample) |
| CA (1) | CA2827880A1 (enExample) |
| RU (1) | RU2636460C2 (enExample) |
| WO (1) | WO2012116191A1 (enExample) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8775454B2 (en) | 2008-07-29 | 2014-07-08 | James L. Geer | Phone assisted ‘photographic memory’ |
| US9128981B1 (en) | 2008-07-29 | 2015-09-08 | James L. Geer | Phone assisted ‘photographic memory’ |
| JP6018092B2 (ja) | 2011-02-23 | 2016-11-02 | イブ バイオメディカル インコーポレイテッド | 回転依存転写配列決定システムおよび使用方法 |
| AU2014218911B2 (en) * | 2013-02-20 | 2018-07-12 | Eve Biomedical, Inc. | Methods and compositions for nanostructure-based nucleic acid sequencing |
| US20160309068A1 (en) * | 2014-01-06 | 2016-10-20 | The Regents Of The University Of California | Spatial frequency domain imaging using custom patterns |
| DK3460075T3 (da) | 2014-07-15 | 2021-02-01 | Illumina Inc | Biokemisk aktiveret elektronisk anordning |
| SG11201708867UA (en) * | 2015-04-30 | 2017-11-29 | Curevac Ag | Immobilized poly(n)polymerase |
| CN106991224A (zh) * | 2017-03-27 | 2017-07-28 | 蔡彤� | 一种芯片引线键合定位的建模方法及系统 |
| US10344328B2 (en) | 2017-11-17 | 2019-07-09 | Ultima Genomics, Inc. | Methods for biological sample processing and analysis |
| US11499962B2 (en) | 2017-11-17 | 2022-11-15 | Ultima Genomics, Inc. | Methods and systems for analyte detection and analysis |
| US10957187B2 (en) * | 2017-12-21 | 2021-03-23 | Lumileds Llc | Road lighting |
| US11798406B2 (en) * | 2018-03-21 | 2023-10-24 | Lumileds Llc | Road lighting |
| US10704094B1 (en) | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
| US10512911B1 (en) | 2018-12-07 | 2019-12-24 | Ultima Genomics, Inc. | Implementing barriers for controlled environments during sample processing and detection |
| US12239980B2 (en) | 2018-12-07 | 2025-03-04 | Ultima Genomics, Inc. | Implementing barriers for controlled environments during sample processing and detection |
| US10852518B1 (en) | 2019-03-14 | 2020-12-01 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US10900078B2 (en) | 2019-03-14 | 2021-01-26 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US10830703B1 (en) | 2019-03-14 | 2020-11-10 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US11118223B2 (en) | 2019-03-14 | 2021-09-14 | Ultima Genomics, Inc. | Methods, devices, and systems for analyte detection and analysis |
| US11609208B2 (en) | 2019-04-12 | 2023-03-21 | Western Digital Technologies, Inc. | Devices and methods for molecule detection based on thermal stabilities of magnetic nanoparticles |
| CN113905822B (zh) * | 2019-04-12 | 2023-09-22 | 西部数据技术公司 | 使用磁传感器阵列边合成边核酸测序 |
| US11327073B2 (en) | 2019-04-12 | 2022-05-10 | Western Digital Technologies, Inc. | Thermal sensor array for molecule detection and related detection schemes |
| US11112468B2 (en) | 2019-04-12 | 2021-09-07 | Western Digital Technologies, Inc. | Magnetoresistive sensor array for molecule detection and related detection schemes |
| CN110376171B (zh) * | 2019-07-15 | 2021-11-19 | 上海理工大学 | 应用于dPCR检测仪的透射式荧光检测成像系统 |
| US11208682B2 (en) | 2019-09-13 | 2021-12-28 | Western Digital Technologies, Inc. | Enhanced optical detection for nucleic acid sequencing using thermally-dependent fluorophore tags |
| US11060138B1 (en) | 2020-01-17 | 2021-07-13 | Element Biosciences, Inc. | Nucleic acid sequencing systems |
| CN116802267A (zh) * | 2020-10-23 | 2023-09-22 | 斯宾德尔生物科技公司 | 用于rna合成的组合物和方法 |
| IL310281A (en) | 2021-07-21 | 2024-03-01 | Element Biosciences Inc | Optical systems for nucleic acid sequencing and methods thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2143004C1 (ru) * | 1993-09-27 | 1999-12-20 | АРЧ Девелопмент Корпорейшн | Способ определения последовательности нуклеиновой кислоты (варианты) и набор для использования при определении последовательности нуклеиновой кислоты |
| US6872816B1 (en) * | 1996-01-24 | 2005-03-29 | Third Wave Technologies, Inc. | Nucleic acid detection kits |
| US20070077575A1 (en) * | 2004-03-05 | 2007-04-05 | Mcallister William T | Use of rna polymerase as an information-dependent molecular motor |
| US20110008794A1 (en) * | 1999-08-30 | 2011-01-13 | The Government of USA represented by the Secretary of the Dept. of Health and Human Services | High speed parallel molecular nucleic acid sequencing |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5693489A (en) | 1984-03-30 | 1997-12-02 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
| US6232075B1 (en) * | 1998-12-14 | 2001-05-15 | Li-Cor, Inc. | Heterogeneous assay for pyrophosphate detection |
| US6529240B2 (en) * | 1999-11-18 | 2003-03-04 | Agilent Technologies, Inc. | Random access memory integrated with CMOS sensors |
| US6548264B1 (en) * | 2000-05-17 | 2003-04-15 | University Of Florida | Coated nanoparticles |
| JP2008507952A (ja) * | 2004-03-05 | 2008-03-21 | リサーチ ファウンデーション オブ ステイト ユニヴァーシティー オブ ニューヨーク | 情報依存性分子モーターとしてのrnaポリメラーゼの使用 |
| WO2007111924A2 (en) * | 2006-03-23 | 2007-10-04 | The Board Of Trustees Of The Leland Stanford Junior University | Motion resolved molecular sequencing |
| AU2008331824B2 (en) * | 2007-12-04 | 2014-07-24 | Pacific Biosciences Of California, Inc. | Alternate labeling strategies for single molecule sequencing |
| US8815576B2 (en) | 2007-12-27 | 2014-08-26 | Lawrence Livermore National Security, Llc. | Chip-based sequencing nucleic acids |
| JP6018092B2 (ja) | 2011-02-23 | 2016-11-02 | イブ バイオメディカル インコーポレイテッド | 回転依存転写配列決定システムおよび使用方法 |
-
2012
- 2012-02-23 JP JP2013555562A patent/JP6018092B2/ja not_active Expired - Fee Related
- 2012-02-23 CA CA2827880A patent/CA2827880A1/en not_active Abandoned
- 2012-02-23 EP EP12709419.1A patent/EP2678444B1/en not_active Not-in-force
- 2012-02-23 KR KR1020137024640A patent/KR101924665B1/ko not_active Expired - Fee Related
- 2012-02-23 CN CN201280019968.8A patent/CN103534357B/zh not_active Expired - Fee Related
- 2012-02-23 US US13/403,645 patent/US8574840B2/en not_active Expired - Fee Related
- 2012-02-23 RU RU2013142965A patent/RU2636460C2/ru not_active IP Right Cessation
- 2012-02-23 AU AU2012220546A patent/AU2012220546B2/en not_active Ceased
- 2012-02-23 WO PCT/US2012/026339 patent/WO2012116191A1/en not_active Ceased
-
2013
- 2013-11-04 US US14/070,904 patent/US9657343B2/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2143004C1 (ru) * | 1993-09-27 | 1999-12-20 | АРЧ Девелопмент Корпорейшн | Способ определения последовательности нуклеиновой кислоты (варианты) и набор для использования при определении последовательности нуклеиновой кислоты |
| US6872816B1 (en) * | 1996-01-24 | 2005-03-29 | Third Wave Technologies, Inc. | Nucleic acid detection kits |
| US20110008794A1 (en) * | 1999-08-30 | 2011-01-13 | The Government of USA represented by the Secretary of the Dept. of Health and Human Services | High speed parallel molecular nucleic acid sequencing |
| US20070077575A1 (en) * | 2004-03-05 | 2007-04-05 | Mcallister William T | Use of rna polymerase as an information-dependent molecular motor |
Non-Patent Citations (1)
| Title |
|---|
| WESTOVER KD, et al., Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center, Cell, 2004, 119 (4), pp.481-489. ДМИТРИЕНКО Е.В., и др., Гибридизационный анализ нуклеиновых кислот с помощью олигонуклеотидных производных. II. Изотермическая амплификация сигнала при анализе ДНК методоом мини-секвенирования, Биоорганическая химия, 2010, том 36, No.6, с.802-814. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2827880A1 (en) | 2012-08-30 |
| US9657343B2 (en) | 2017-05-23 |
| CN103534357B (zh) | 2016-05-18 |
| CN103534357A (zh) | 2014-01-22 |
| EP2678444B1 (en) | 2016-02-17 |
| AU2012220546B2 (en) | 2017-02-23 |
| KR101924665B1 (ko) | 2018-12-03 |
| RU2013142965A (ru) | 2015-03-27 |
| EP2678444A1 (en) | 2014-01-01 |
| JP6018092B2 (ja) | 2016-11-02 |
| US8574840B2 (en) | 2013-11-05 |
| WO2012116191A1 (en) | 2012-08-30 |
| HK1187959A1 (zh) | 2014-04-17 |
| KR20140089308A (ko) | 2014-07-14 |
| US20140162275A1 (en) | 2014-06-12 |
| AU2012220546A1 (en) | 2013-09-12 |
| US20120214171A1 (en) | 2012-08-23 |
| JP2014506485A (ja) | 2014-03-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2636460C2 (ru) | Системы и способы применения зависящего от вращения транскрипционного секвенирования | |
| Pyrak et al. | Surface enhanced Raman spectroscopy for DNA biosensors—How far are we? | |
| Soares et al. | Rolling circle amplification in integrated microsystems: An uncut gem toward massively multiplexed pathogen diagnostics and genotyping | |
| Dubus et al. | PCR-free DNA detection using a magnetic bead-supported polymeric transducer and microelectromagnetic traps | |
| AU2014218911B2 (en) | Methods and compositions for nanostructure-based nucleic acid sequencing | |
| JP6620160B2 (ja) | 単一細胞の迅速かつ正確な分注、視覚化及び解析のための方法 | |
| CN116323974A (zh) | 多路复用covid-19锁式测定 | |
| JP2017533696A (ja) | ナノ構造のアレイを使用するポリマーの効率的光学分析 | |
| CN105264088A (zh) | 提高鉴定细胞中的多个表位的动态范围 | |
| CN107109472B (zh) | 利用互相猝灭的荧光标记物的基于纳米孔的聚合物分析 | |
| Chun et al. | Salmonella Typhimurium sensing strategy based on the loop-mediated isothermal amplification using retroreflective janus particle as a nonspectroscopic signaling probe | |
| Alamri et al. | Era of molecular diagnostics techniques before and after the COVID-19 pandemic | |
| Qu et al. | Real-time continuous identification of greenhouse plant pathogens based on recyclable microfluidic bioassay system | |
| Azimzadeh et al. | CRISPR-powered microfluidics in diagnostics: a review of main applications | |
| JP2019054805A (ja) | 核酸を配列決定する方法および装置 | |
| Yu et al. | CRISPR-Cas-and aptamer-based systems for diagnosing pathogens: A Review | |
| Presnova et al. | Multiplex digital quantification of β-lactamase genes in antibiotic-resistant bacteria by counting gold nanoparticle labels on silicon microchips | |
| Cui et al. | Simultaneous and sensitive detection of dual DNA targets via quantum dot‐assembled amplification labels | |
| HK1187959B (en) | Rotation-dependent transcriptional sequencing systems and methods of using | |
| Masoudi-Nejad et al. | Emergence of Next-Generation Sequencing | |
| Saunders | Application of nanomaterials to arrays for infectious disease diagnosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20180224 |