JP2016507249A5 - - Google Patents
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- Publication number
- JP2016507249A5 JP2016507249A5 JP2015558959A JP2015558959A JP2016507249A5 JP 2016507249 A5 JP2016507249 A5 JP 2016507249A5 JP 2015558959 A JP2015558959 A JP 2015558959A JP 2015558959 A JP2015558959 A JP 2015558959A JP 2016507249 A5 JP2016507249 A5 JP 2016507249A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- acid molecule
- target nucleic
- polymerase
- sequencing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims description 84
- 108020004707 nucleic acids Proteins 0.000 claims description 77
- 150000007523 nucleic acids Chemical class 0.000 claims description 77
- 102000039446 nucleic acids Human genes 0.000 claims description 77
- 238000012163 sequencing technique Methods 0.000 claims description 65
- 239000002777 nucleoside Substances 0.000 claims description 62
- -1 nucleoside triphosphate Chemical class 0.000 claims description 62
- 235000011178 triphosphate Nutrition 0.000 claims description 62
- 239000001226 triphosphate Substances 0.000 claims description 62
- 239000002086 nanomaterial Substances 0.000 claims description 37
- 239000007787 solid Substances 0.000 claims description 28
- 239000000758 substrate Substances 0.000 claims description 21
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 15
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 11
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 9
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 9
- 108020004513 Bacterial RNA Proteins 0.000 claims description 4
- 241001515965 unidentified phage Species 0.000 claims description 4
- 239000012472 biological sample Substances 0.000 claims description 3
- 230000005012 migration Effects 0.000 claims description 3
- 238000013508 migration Methods 0.000 claims description 3
- 239000002071 nanotube Substances 0.000 claims description 3
- 239000002070 nanowire Substances 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 claims description 2
- 101710137500 T7 RNA polymerase Proteins 0.000 claims description 2
- 108010006785 Taq Polymerase Proteins 0.000 claims description 2
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 description 10
- 239000000872 buffer Substances 0.000 description 8
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000012148 binding buffer Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361766925P | 2013-02-20 | 2013-02-20 | |
| US61/766,925 | 2013-02-20 | ||
| PCT/US2014/017419 WO2014130686A2 (en) | 2013-02-20 | 2014-02-20 | Methods and compositions for nanostructure-based nucleic acid sequencing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016507249A JP2016507249A (ja) | 2016-03-10 |
| JP2016507249A5 true JP2016507249A5 (enExample) | 2017-03-16 |
Family
ID=51351616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015558959A Pending JP2016507249A (ja) | 2013-02-20 | 2014-02-20 | ナノ構造ベースの核酸配列決定のための方法および組成物 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US9725763B2 (enExample) |
| EP (1) | EP2958927A4 (enExample) |
| JP (1) | JP2016507249A (enExample) |
| KR (1) | KR20150119353A (enExample) |
| CN (1) | CN105308062A (enExample) |
| AU (1) | AU2014218911B2 (enExample) |
| CA (1) | CA2900921A1 (enExample) |
| HK (1) | HK1221227A1 (enExample) |
| RU (1) | RU2015139609A (enExample) |
| WO (1) | WO2014130686A2 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012264497B2 (en) | 2011-05-27 | 2017-06-15 | Oxford Nanopore Technologies Limited | Coupling method |
| CN111187811B (zh) | 2013-05-06 | 2024-03-08 | 加利福尼亚太平洋生物科学股份有限公司 | 实时电子测序 |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| EP3126515B1 (en) | 2014-04-04 | 2018-08-29 | Oxford Nanopore Technologies Limited | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| WO2017024049A1 (en) | 2015-08-06 | 2017-02-09 | Pacific Biosciences Of California, Inc. | Single-molecule nanofet sequencing systems and methods |
| CN105170209B (zh) * | 2015-10-15 | 2017-05-10 | 华中科技大学 | 一种表面图案化修饰的基片及其制备方法 |
| CN109071212A (zh) * | 2016-01-28 | 2018-12-21 | 罗斯韦尔生物技术股份有限公司 | 使用大规模分子电子传感器阵列测量分析物的方法和装置 |
| CN110168109B (zh) * | 2016-08-04 | 2022-09-20 | 致优制药株式会社 | 检测及鉴定结核菌和非结核菌感染的诊断方法及结核杆菌对利凡平耐药性的确认及其试剂组 |
| CN106244712B (zh) * | 2016-08-31 | 2019-11-19 | 北京大学 | Dna测序方法 |
| EP3571286A4 (en) * | 2017-01-19 | 2020-10-28 | Roswell Biotechnologies, Inc | SEMICONDUCTOR SEQUENCING DEVICES INCLUDING TWO-DIMENSIONAL LAYER MATERIALS |
| JP6559814B2 (ja) * | 2018-01-26 | 2019-08-14 | 株式会社日立ハイテクノロジーズ | 生体分子測定方法 |
| CA3104272A1 (en) * | 2019-06-18 | 2020-12-24 | Illumina, Inc. | Polymerase attachment to a conductive channel |
| CN110452817A (zh) * | 2019-07-08 | 2019-11-15 | 广东工业大学 | 一种dna测序装置及测序方法 |
| CN111455034B (zh) * | 2020-04-09 | 2024-04-09 | 苏州罗岛纳米科技有限公司 | 一种基于固态纳米孔机构的单分子检测方法和系统 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5693489A (en) | 1984-03-30 | 1997-12-02 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
| US5795782A (en) * | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US20020172953A1 (en) * | 1996-07-29 | 2002-11-21 | Mirkin Chad A. | Movement of biomolecule-coated nanoparticles in an electric field |
| US6232075B1 (en) | 1998-12-14 | 2001-05-15 | Li-Cor, Inc. | Heterogeneous assay for pyrophosphate detection |
| US6529240B2 (en) | 1999-11-18 | 2003-03-04 | Agilent Technologies, Inc. | Random access memory integrated with CMOS sensors |
| US7238477B2 (en) | 2001-09-24 | 2007-07-03 | Intel Corporation | Methods to increase nucleotide signals by Raman scattering |
| ATE432365T1 (de) * | 2003-02-21 | 2009-06-15 | Geneform Technologies Ltd | Verfahren, kits und reagenzien zur nukleinsäuresequenzierung |
| US20070077575A1 (en) | 2004-03-05 | 2007-04-05 | Mcallister William T | Use of rna polymerase as an information-dependent molecular motor |
| WO2006028508A2 (en) * | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2007111924A2 (en) * | 2006-03-23 | 2007-10-04 | The Board Of Trustees Of The Leland Stanford Junior University | Motion resolved molecular sequencing |
| AU2008236694B2 (en) * | 2007-04-04 | 2014-01-23 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| US9395352B2 (en) * | 2007-04-06 | 2016-07-19 | Arizona Board Of Regents On Behalf Of Arizona State University | Devices and methods for target molecule characterization |
| AU2008331824B2 (en) | 2007-12-04 | 2014-07-24 | Pacific Biosciences Of California, Inc. | Alternate labeling strategies for single molecule sequencing |
| US8815576B2 (en) | 2007-12-27 | 2014-08-26 | Lawrence Livermore National Security, Llc. | Chip-based sequencing nucleic acids |
| WO2009117522A2 (en) * | 2008-03-18 | 2009-09-24 | Reinhart, Kevin | Nanopore and carbon nanotube based dna sequencer and a serial recognition sequencer |
| US20100261185A1 (en) * | 2009-03-27 | 2010-10-14 | Life Technologies Corporation | Labeled enzyme compositions, methods and systems |
| US9017937B1 (en) * | 2009-04-10 | 2015-04-28 | Pacific Biosciences Of California, Inc. | Nanopore sequencing using ratiometric impedance |
| CA2790672C (en) * | 2010-02-23 | 2018-05-29 | University Of Washington | Artificial mycolic acid membranes |
| WO2012033524A2 (en) * | 2010-09-07 | 2012-03-15 | The Regents Of The University Of California | Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme |
| US10443096B2 (en) * | 2010-12-17 | 2019-10-15 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by synthesis using modified nucleotides and nanopore detection |
| JP6018092B2 (ja) * | 2011-02-23 | 2016-11-02 | イブ バイオメディカル インコーポレイテッド | 回転依存転写配列決定システムおよび使用方法 |
| AU2012264497B2 (en) | 2011-05-27 | 2017-06-15 | Oxford Nanopore Technologies Limited | Coupling method |
| JP6298404B2 (ja) * | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| US9164053B2 (en) * | 2011-09-26 | 2015-10-20 | The Regents Of The University Of California | Electronic device for monitoring single molecule dynamics |
| US9279154B2 (en) * | 2011-12-21 | 2016-03-08 | Illumina, Inc. | Apparatus and methods for kinetic analysis and determination of nucleic acid sequences |
| WO2013185137A1 (en) * | 2012-06-08 | 2013-12-12 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| US20140147833A1 (en) * | 2012-11-28 | 2014-05-29 | International Business Machines Corporation | Base recognition based on the conformation change of a motor molecule |
-
2014
- 2014-02-20 AU AU2014218911A patent/AU2014218911B2/en not_active Ceased
- 2014-02-20 RU RU2015139609A patent/RU2015139609A/ru not_active Application Discontinuation
- 2014-02-20 EP EP14754640.2A patent/EP2958927A4/en not_active Withdrawn
- 2014-02-20 JP JP2015558959A patent/JP2016507249A/ja active Pending
- 2014-02-20 CA CA2900921A patent/CA2900921A1/en not_active Abandoned
- 2014-02-20 HK HK16109275.9A patent/HK1221227A1/zh unknown
- 2014-02-20 CN CN201480022008.6A patent/CN105308062A/zh active Pending
- 2014-02-20 WO PCT/US2014/017419 patent/WO2014130686A2/en not_active Ceased
- 2014-02-20 US US14/185,469 patent/US9725763B2/en active Active
- 2014-02-20 KR KR1020157025411A patent/KR20150119353A/ko not_active Withdrawn
-
2017
- 2017-08-02 US US15/666,671 patent/US20180010181A1/en not_active Abandoned
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