CN105308062A - 用于基于纳米结构的核酸测序的方法和组合物 - Google Patents

用于基于纳米结构的核酸测序的方法和组合物 Download PDF

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Publication number
CN105308062A
CN105308062A CN201480022008.6A CN201480022008A CN105308062A CN 105308062 A CN105308062 A CN 105308062A CN 201480022008 A CN201480022008 A CN 201480022008A CN 105308062 A CN105308062 A CN 105308062A
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nucleic acid
target nucleic
acid molecule
nanostructure
order
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Chinese (zh)
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T.科特塞罗格洛
S.帕帕德米特里奥
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Biomedical Limited-Liability Co Of Eve
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Biomedical Limited-Liability Co Of Eve
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201480022008.6A 2013-02-20 2014-02-20 用于基于纳米结构的核酸测序的方法和组合物 Pending CN105308062A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361766925P 2013-02-20 2013-02-20
US61/766,925 2013-02-20
PCT/US2014/017419 WO2014130686A2 (en) 2013-02-20 2014-02-20 Methods and compositions for nanostructure-based nucleic acid sequencing

Publications (1)

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CN105308062A true CN105308062A (zh) 2016-02-03

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CN201480022008.6A Pending CN105308062A (zh) 2013-02-20 2014-02-20 用于基于纳米结构的核酸测序的方法和组合物

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Country Link
US (2) US9725763B2 (enExample)
EP (1) EP2958927A4 (enExample)
JP (1) JP2016507249A (enExample)
KR (1) KR20150119353A (enExample)
CN (1) CN105308062A (enExample)
AU (1) AU2014218911B2 (enExample)
CA (1) CA2900921A1 (enExample)
HK (1) HK1221227A1 (enExample)
RU (1) RU2015139609A (enExample)
WO (1) WO2014130686A2 (enExample)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110168109A (zh) * 2016-08-04 2019-08-23 致优制药株式会社 检测及鉴定结核菌和非结核菌感染的诊断方法及结核杆菌对利凡平耐药性的确认及其试剂组
CN110452817A (zh) * 2019-07-08 2019-11-15 广东工业大学 一种dna测序装置及测序方法
CN110520517A (zh) * 2017-01-19 2019-11-29 罗斯威尔生命技术公司 包括二维层材料的固态测序装置
CN111455034A (zh) * 2020-04-09 2020-07-28 南京罗岛纳米科技有限公司 一种基于固态纳米孔机构的单分子检测方法和系统
CN112673087A (zh) * 2019-06-18 2021-04-16 伊鲁米纳公司 聚合酶至导电通道的附接

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
US9708656B2 (en) 2013-05-06 2017-07-18 Pacific Biosciences Of California, Inc. Real-time electronic sequencing
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN106460061B (zh) 2014-04-04 2020-03-06 牛津纳米孔技术公司 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
CN108139345B (zh) 2015-08-06 2021-04-20 加利福尼亚太平洋生物科学股份有限公司 用于选择性寻址稀疏布置的电子测量装置的系统和方法
CN105170209B (zh) * 2015-10-15 2017-05-10 华中科技大学 一种表面图案化修饰的基片及其制备方法
JP7280590B2 (ja) * 2016-01-28 2023-05-24 ロズウェル バイオテクノロジーズ，インコーポレイテッド 大スケールの分子電子工学センサアレイを使用する被分析物を測定するための方法および装置
CN106244712B (zh) * 2016-08-31 2019-11-19 北京大学 Dna测序方法
JP6559814B2 (ja) * 2018-01-26 2019-08-14 株式会社日立ハイテクノロジーズ 生体分子測定方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020172953A1 (en) * 1996-07-29 2002-11-21 Mirkin Chad A. Movement of biomolecule-coated nanoparticles in an electric field
CN1894421A (zh) * 2003-09-12 2007-01-10 英特尔公司 通过拉曼散射增强核苷酸信号的方法
US20110003343A1 (en) * 2009-03-27 2011-01-06 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
WO2012083249A2 (en) * 2010-12-17 2012-06-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
US20120214171A1 (en) * 2011-02-23 2012-08-23 Theofilos Kotseroglou Rotation-dependent transcriptional sequencing systems and methods of using
WO2012164270A1 (en) * 2011-05-27 2012-12-06 Oxford Nanopore Technologies Limited Coupling method

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693489A (en) 1984-03-30 1997-12-02 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
AU772281B2 (en) 1998-12-14 2004-04-22 Li-Cor Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6529240B2 (en) 1999-11-18 2003-03-04 Agilent Technologies, Inc. Random access memory integrated with CMOS sensors
US8399196B2 (en) * 2003-02-21 2013-03-19 Geneform Technologies Limited Nucleic acid sequencing methods, kits and reagents
US20070077575A1 (en) 2004-03-05 2007-04-05 Mcallister William T Use of rna polymerase as an information-dependent molecular motor
US7238485B2 (en) * 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2007111924A2 (en) * 2006-03-23 2007-10-04 The Board Of Trustees Of The Leland Stanford Junior University Motion resolved molecular sequencing
US20110005918A1 (en) * 2007-04-04 2011-01-13 Akeson Mark A Compositions, devices, systems, and methods for using a nanopore
WO2008124706A2 (en) * 2007-04-06 2008-10-16 Arizona Board Of Regents Acting For And On Behalf Of Arizona State University Devices and methods for target molecule characterization
US20090208957A1 (en) 2007-12-04 2009-08-20 Pacific Biosciences Of California, Inc. Alternate labeling strategies for single molecule sequencing
US8815576B2 (en) 2007-12-27 2014-08-26 Lawrence Livermore National Security, Llc. Chip-based sequencing nucleic acids
WO2009117522A2 (en) * 2008-03-18 2009-09-24 Reinhart, Kevin Nanopore and carbon nanotube based dna sequencer and a serial recognition sequencer
US9017937B1 (en) * 2009-04-10 2015-04-28 Pacific Biosciences Of California, Inc. Nanopore sequencing using ratiometric impedance
CN102834527B (zh) * 2010-02-23 2015-06-03 华盛顿大学 用纳米孔的分析物测序
WO2012033524A2 (en) * 2010-09-07 2012-03-15 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
WO2013014451A1 (en) * 2011-07-25 2013-01-31 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US9164053B2 (en) * 2011-09-26 2015-10-20 The Regents Of The University Of California Electronic device for monitoring single molecule dynamics
US9279154B2 (en) * 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
GB2557509A (en) * 2012-06-08 2018-06-20 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
US20140147833A1 (en) * 2012-11-28 2014-05-29 International Business Machines Corporation Base recognition based on the conformation change of a motor molecule

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020172953A1 (en) * 1996-07-29 2002-11-21 Mirkin Chad A. Movement of biomolecule-coated nanoparticles in an electric field
CN1894421A (zh) * 2003-09-12 2007-01-10 英特尔公司 通过拉曼散射增强核苷酸信号的方法
US20110003343A1 (en) * 2009-03-27 2011-01-06 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
WO2012083249A2 (en) * 2010-12-17 2012-06-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
US20120214171A1 (en) * 2011-02-23 2012-08-23 Theofilos Kotseroglou Rotation-dependent transcriptional sequencing systems and methods of using
WO2012164270A1 (en) * 2011-05-27 2012-12-06 Oxford Nanopore Technologies Limited Coupling method

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110168109A (zh) * 2016-08-04 2019-08-23 致优制药株式会社 检测及鉴定结核菌和非结核菌感染的诊断方法及结核杆菌对利凡平耐药性的确认及其试剂组
CN110168109B (zh) * 2016-08-04 2022-09-20 致优制药株式会社 检测及鉴定结核菌和非结核菌感染的诊断方法及结核杆菌对利凡平耐药性的确认及其试剂组
CN110520517A (zh) * 2017-01-19 2019-11-29 罗斯威尔生命技术公司 包括二维层材料的固态测序装置
CN112673087A (zh) * 2019-06-18 2021-04-16 伊鲁米纳公司 聚合酶至导电通道的附接
CN110452817A (zh) * 2019-07-08 2019-11-15 广东工业大学 一种dna测序装置及测序方法
CN111455034A (zh) * 2020-04-09 2020-07-28 南京罗岛纳米科技有限公司 一种基于固态纳米孔机构的单分子检测方法和系统
CN111455034B (zh) * 2020-04-09 2024-04-09 苏州罗岛纳米科技有限公司 一种基于固态纳米孔机构的单分子检测方法和系统

Also Published As

Publication number Publication date
CA2900921A1 (en) 2014-08-28
RU2015139609A (ru) 2017-03-24
AU2014218911A1 (en) 2015-08-27
JP2016507249A (ja) 2016-03-10
EP2958927A2 (en) 2015-12-30
US20140235462A1 (en) 2014-08-21
WO2014130686A2 (en) 2014-08-28
EP2958927A4 (en) 2016-09-14
AU2014218911B2 (en) 2018-07-12
KR20150119353A (ko) 2015-10-23
WO2014130686A3 (en) 2014-10-09
HK1221227A1 (zh) 2017-05-26
US9725763B2 (en) 2017-08-08
US20180010181A1 (en) 2018-01-11

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