KR20150119353A - 나노구조-기반 핵산 서열분석을 위한 방법 및 조성물 - Google Patents

나노구조-기반 핵산 서열분석을 위한 방법 및 조성물 Download PDF

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KR20150119353A
KR20150119353A KR1020157025411A KR20157025411A KR20150119353A KR 20150119353 A KR20150119353 A KR 20150119353A KR 1020157025411 A KR1020157025411 A KR 1020157025411A KR 20157025411 A KR20157025411 A KR 20157025411A KR 20150119353 A KR20150119353 A KR 20150119353A
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South Korea
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nucleic acid
acid molecule
target nucleic
nanostructure
polymerase
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Korean (ko)
Inventor
테로필로스 코트세로글루
스테파노스 파파디미트리우
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이브 바이오메디컬, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/119—RNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/543—Immobilised enzyme(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143—Magnetism, e.g. magnetic label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020157025411A 2013-02-20 2014-02-20 나노구조-기반 핵산 서열분석을 위한 방법 및 조성물 Withdrawn KR20150119353A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361766925P 2013-02-20 2013-02-20
US61/766,925 2013-02-20
PCT/US2014/017419 WO2014130686A2 (en) 2013-02-20 2014-02-20 Methods and compositions for nanostructure-based nucleic acid sequencing

Publications (1)

Publication Number Publication Date
KR20150119353A true KR20150119353A (ko) 2015-10-23

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KR1020157025411A Withdrawn KR20150119353A (ko) 2013-02-20 2014-02-20 나노구조-기반 핵산 서열분석을 위한 방법 및 조성물

Country Status (10)

Country Link
US (2) US9725763B2 (enExample)
EP (1) EP2958927A4 (enExample)
JP (1) JP2016507249A (enExample)
KR (1) KR20150119353A (enExample)
CN (1) CN105308062A (enExample)
AU (1) AU2014218911B2 (enExample)
CA (1) CA2900921A1 (enExample)
HK (1) HK1221227A1 (enExample)
RU (1) RU2015139609A (enExample)
WO (1) WO2014130686A2 (enExample)

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EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
WO2017024049A1 (en) 2015-08-06 2017-02-09 Pacific Biosciences Of California, Inc. Single-molecule nanofet sequencing systems and methods
CN105170209B (zh) * 2015-10-15 2017-05-10 华中科技大学 一种表面图案化修饰的基片及其制备方法
CN109071212A (zh) * 2016-01-28 2018-12-21 罗斯韦尔生物技术股份有限公司 使用大规模分子电子传感器阵列测量分析物的方法和装置
CN110168109B (zh) * 2016-08-04 2022-09-20 致优制药株式会社 检测及鉴定结核菌和非结核菌感染的诊断方法及结核杆菌对利凡平耐药性的确认及其试剂组
CN106244712B (zh) * 2016-08-31 2019-11-19 北京大学 Dna测序方法
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JP6559814B2 (ja) * 2018-01-26 2019-08-14 株式会社日立ハイテクノロジーズ 生体分子測定方法
CA3104272A1 (en) * 2019-06-18 2020-12-24 Illumina, Inc. Polymerase attachment to a conductive channel
CN110452817A (zh) * 2019-07-08 2019-11-15 广东工业大学 一种dna测序装置及测序方法
CN111455034B (zh) * 2020-04-09 2024-04-09 苏州罗岛纳米科技有限公司 一种基于固态纳米孔机构的单分子检测方法和系统

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US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20020172953A1 (en) * 1996-07-29 2002-11-21 Mirkin Chad A. Movement of biomolecule-coated nanoparticles in an electric field
US6232075B1 (en) 1998-12-14 2001-05-15 Li-Cor, Inc. Heterogeneous assay for pyrophosphate detection
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ATE432365T1 (de) * 2003-02-21 2009-06-15 Geneform Technologies Ltd Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
US20070077575A1 (en) 2004-03-05 2007-04-05 Mcallister William T Use of rna polymerase as an information-dependent molecular motor
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Also Published As

Publication number Publication date
CN105308062A (zh) 2016-02-03
RU2015139609A (ru) 2017-03-24
US9725763B2 (en) 2017-08-08
AU2014218911A1 (en) 2015-08-27
EP2958927A2 (en) 2015-12-30
AU2014218911B2 (en) 2018-07-12
JP2016507249A (ja) 2016-03-10
HK1221227A1 (zh) 2017-05-26
US20180010181A1 (en) 2018-01-11
WO2014130686A2 (en) 2014-08-28
EP2958927A4 (en) 2016-09-14
WO2014130686A3 (en) 2014-10-09
CA2900921A1 (en) 2014-08-28
US20140235462A1 (en) 2014-08-21

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PA0105 International application

Patent event date: 20150916

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
PC1203 Withdrawal of no request for examination
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid