JP2012530782A5 - - Google Patents
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- Publication number
- JP2012530782A5 JP2012530782A5 JP2012517634A JP2012517634A JP2012530782A5 JP 2012530782 A5 JP2012530782 A5 JP 2012530782A5 JP 2012517634 A JP2012517634 A JP 2012517634A JP 2012517634 A JP2012517634 A JP 2012517634A JP 2012530782 A5 JP2012530782 A5 JP 2012530782A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- group
- thiol
- refolding
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 17
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 6
- 235000018102 proteins Nutrition 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 150000003573 thiols Chemical class 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 5
- 239000011159 matrix material Substances 0.000 claims 4
- 238000000926 separation method Methods 0.000 claims 4
- 239000000872 buffer Substances 0.000 claims 3
- 238000002955 isolation Methods 0.000 claims 3
- 239000004475 Arginine Substances 0.000 claims 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 2
- 239000002202 Polyethylene glycol Substances 0.000 claims 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 2
- 229930006000 Sucrose Natural products 0.000 claims 2
- 239000007983 Tris buffer Substances 0.000 claims 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 2
- 238000004220 aggregation Methods 0.000 claims 2
- 230000002776 aggregation Effects 0.000 claims 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 238000001914 filtration Methods 0.000 claims 2
- 239000008103 glucose Substances 0.000 claims 2
- 239000003112 inhibitor Substances 0.000 claims 2
- 239000002563 ionic surfactant Substances 0.000 claims 2
- 239000002736 nonionic surfactant Substances 0.000 claims 2
- 230000000065 osmolyte Effects 0.000 claims 2
- 229920001223 polyethylene glycol Polymers 0.000 claims 2
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims 2
- 229910052939 potassium sulfate Inorganic materials 0.000 claims 2
- 235000011151 potassium sulphates Nutrition 0.000 claims 2
- 229940124272 protein stabilizer Drugs 0.000 claims 2
- 229910052938 sodium sulfate Inorganic materials 0.000 claims 2
- 235000011152 sodium sulphate Nutrition 0.000 claims 2
- 239000000600 sorbitol Substances 0.000 claims 2
- 239000005720 sucrose Substances 0.000 claims 2
- 150000005846 sugar alcohols Polymers 0.000 claims 2
- RYECOJGRJDOGPP-UHFFFAOYSA-N Ethylurea Chemical compound CCNC(N)=O RYECOJGRJDOGPP-UHFFFAOYSA-N 0.000 claims 1
- 108010024636 Glutathione Proteins 0.000 claims 1
- 108010053070 Glutathione Disulfide Proteins 0.000 claims 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical class NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 claims 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims 1
- MGJKQDOBUOMPEZ-UHFFFAOYSA-N N,N'-dimethylurea Chemical compound CNC(=O)NC MGJKQDOBUOMPEZ-UHFFFAOYSA-N 0.000 claims 1
- XGEGHDBEHXKFPX-UHFFFAOYSA-N N-methylthiourea Natural products CNC(N)=O XGEGHDBEHXKFPX-UHFFFAOYSA-N 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- OOTFVKOQINZBBF-UHFFFAOYSA-N cystamine Chemical compound CCSSCCN OOTFVKOQINZBBF-UHFFFAOYSA-N 0.000 claims 1
- 229940099500 cystamine Drugs 0.000 claims 1
- UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 1
- 235000018417 cysteine Nutrition 0.000 claims 1
- 229960002433 cysteine Drugs 0.000 claims 1
- 229960003067 cystine Drugs 0.000 claims 1
- 238000011118 depth filtration Methods 0.000 claims 1
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 1
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 claims 1
- 210000003000 inclusion body Anatomy 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 238000005342 ion exchange Methods 0.000 claims 1
- 229960003151 mercaptamine Drugs 0.000 claims 1
- XGEGHDBEHXKFPX-NJFSPNSNSA-N methylurea Chemical compound [14CH3]NC(N)=O XGEGHDBEHXKFPX-NJFSPNSNSA-N 0.000 claims 1
- YPZRWBKMTBYPTK-UHFFFAOYSA-N oxidized gamma-L-glutamyl-L-cysteinylglycine Natural products OC(=O)C(N)CCC(=O)NC(C(=O)NCC(O)=O)CSSCC(C(=O)NCC(O)=O)NC(=O)CCC(N)C(O)=O YPZRWBKMTBYPTK-UHFFFAOYSA-N 0.000 claims 1
- 239000012562 protein A resin Substances 0.000 claims 1
- 239000006176 redox buffer Substances 0.000 claims 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21925709P | 2009-06-22 | 2009-06-22 | |
| US61/219,257 | 2009-06-22 | ||
| PCT/US2010/039390 WO2011005488A1 (en) | 2009-06-22 | 2010-06-21 | Refolding proteins using a chemically controlled redox state |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015175422A Division JP6026608B2 (ja) | 2009-06-22 | 2015-09-07 | 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012530782A JP2012530782A (ja) | 2012-12-06 |
| JP2012530782A5 true JP2012530782A5 (enExample) | 2013-07-18 |
| JP5808323B2 JP5808323B2 (ja) | 2015-11-10 |
Family
ID=42710673
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012517634A Active JP5808323B2 (ja) | 2009-06-22 | 2010-06-21 | 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング |
| JP2015175422A Active JP6026608B2 (ja) | 2009-06-22 | 2015-09-07 | 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015175422A Active JP6026608B2 (ja) | 2009-06-22 | 2015-09-07 | 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング |
Country Status (15)
| Country | Link |
|---|---|
| US (5) | US8952138B2 (enExample) |
| EP (2) | EP3366692A1 (enExample) |
| JP (2) | JP5808323B2 (enExample) |
| KR (1) | KR101741859B1 (enExample) |
| CN (1) | CN102482321B (enExample) |
| AU (1) | AU2010270986B2 (enExample) |
| BR (1) | BRPI1011940B8 (enExample) |
| CA (1) | CA2765881C (enExample) |
| CL (1) | CL2011003278A1 (enExample) |
| EA (1) | EA020621B1 (enExample) |
| IL (1) | IL216954A (enExample) |
| MX (1) | MX2011013898A (enExample) |
| SG (1) | SG176963A1 (enExample) |
| WO (1) | WO2011005488A1 (enExample) |
| ZA (1) | ZA201200512B (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5808323B2 (ja) * | 2009-06-22 | 2015-11-10 | アムジエン・インコーポレーテツド | 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング |
| EP3660032B1 (en) | 2009-06-25 | 2025-10-22 | Amgen Inc. | Capture purification processes for proteins expressed in a non-mammalian system |
| EP2417982A1 (en) * | 2010-07-30 | 2012-02-15 | Ferring B.V. | Stabilization of gonadotropins |
| US20150376228A1 (en) * | 2013-02-22 | 2015-12-31 | Biogenomics Limited | Process for high efficiency refolding of recombinant proteins |
| WO2017019776A1 (en) * | 2015-07-27 | 2017-02-02 | Purdue Research Foundation | Tandem folding methods to improve protein folding yield |
| EP3344651B1 (en) | 2015-09-02 | 2022-03-02 | Merck Sharp & Dohme Corp. | A process for obtaining insulin with correctly formed disulfide bonds |
| IL285146B (en) * | 2016-07-22 | 2022-09-01 | Amgen Inc | Methods for purifying proteins involving fc |
| CN108276470A (zh) * | 2017-01-06 | 2018-07-13 | 深圳市新产业生物医学工程股份有限公司 | 细胞裂解液、提取胞内蛋白的方法、制备弓形虫抗原的方法和试剂盒 |
| US11406576B2 (en) * | 2017-04-10 | 2022-08-09 | Kao Corporation | Method for removing keratotic plugs |
| CN119881293A (zh) | 2018-03-21 | 2025-04-25 | 沃特世科技公司 | 基于非抗体高亲和力的样品制备、吸附剂、装置和方法 |
| KR20210110294A (ko) * | 2018-11-07 | 2021-09-07 | 어플라이드 몰레큘라 트랜스포트 인크. | 통과세포외배출용 전달 구조체 및 관련 방법 |
| AU2021399935A1 (en) | 2020-12-18 | 2023-06-29 | Richter Gedeon Nyrt. | Methods for the purification of refolded fc-peptide fusion protein |
| CN115894604B (zh) * | 2022-12-16 | 2024-01-23 | 康日百奥生物科技(苏州)有限公司 | 重组蛋白澄清纯化方法 |
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| CN105530949A (zh) | 2013-03-15 | 2016-04-27 | 安姆根有限公司 | 人类受试者中的肌抑素拮抗作用 |
| US9632095B2 (en) | 2014-12-01 | 2017-04-25 | University Of Delaware | Device and method for determining reaction kinetics |
| US9704239B1 (en) | 2016-09-02 | 2017-07-11 | Amgen Inc. | Video trigger synchronization for improved particle detection in a vessel |
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