JP5808323B2 - 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング - Google Patents

化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング Download PDF

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JP5808323B2
JP5808323B2 JP2012517634A JP2012517634A JP5808323B2 JP 5808323 B2 JP5808323 B2 JP 5808323B2 JP 2012517634 A JP2012517634 A JP 2012517634A JP 2012517634 A JP2012517634 A JP 2012517634A JP 5808323 B2 JP5808323 B2 JP 5808323B2
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protein
thiol
refolding
buffer
contrast
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JP2012530782A (ja
JP2012530782A5 (enExample
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シユルツ，ジヨゼフ・エドワード
ハート，ロジヤー
キーナー・ザ・サード，ロナルド・ニクソン
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アムジエン・インコーポレーテツド
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2012517634A 2009-06-22 2010-06-21 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング Active JP5808323B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21925709P 2009-06-22 2009-06-22
US61/219,257 2009-06-22
PCT/US2010/039390 WO2011005488A1 (en) 2009-06-22 2010-06-21 Refolding proteins using a chemically controlled redox state

Related Child Applications (1)

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JP2015175422A Division JP6026608B2 (ja) 2009-06-22 2015-09-07 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング

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JP2012530782A JP2012530782A (ja) 2012-12-06
JP2012530782A5 JP2012530782A5 (enExample) 2013-07-18
JP5808323B2 true JP5808323B2 (ja) 2015-11-10

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JP2012517634A Active JP5808323B2 (ja) 2009-06-22 2010-06-21 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング
JP2015175422A Active JP6026608B2 (ja) 2009-06-22 2015-09-07 化学的に制御されたレドックス状態を用いたタンパク質のリフォールディング

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US (5) US8952138B2 (enExample)
EP (2) EP3366692A1 (enExample)
JP (2) JP5808323B2 (enExample)
KR (1) KR101741859B1 (enExample)
CN (1) CN102482321B (enExample)
AU (1) AU2010270986B2 (enExample)
BR (1) BRPI1011940B8 (enExample)
CA (1) CA2765881C (enExample)
CL (1) CL2011003278A1 (enExample)
EA (1) EA020621B1 (enExample)
IL (1) IL216954A (enExample)
MX (1) MX2011013898A (enExample)
SG (1) SG176963A1 (enExample)
WO (1) WO2011005488A1 (enExample)
ZA (1) ZA201200512B (enExample)

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WO2017019776A1 (en) * 2015-07-27 2017-02-02 Purdue Research Foundation Tandem folding methods to improve protein folding yield
EP3344651B1 (en) 2015-09-02 2022-03-02 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds
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CN108276470A (zh) * 2017-01-06 2018-07-13 深圳市新产业生物医学工程股份有限公司 细胞裂解液、提取胞内蛋白的方法、制备弓形虫抗原的方法和试剂盒
US11406576B2 (en) * 2017-04-10 2022-08-09 Kao Corporation Method for removing keratotic plugs
CN119881293A (zh) 2018-03-21 2025-04-25 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
KR20210110294A (ko) * 2018-11-07 2021-09-07 어플라이드 몰레큘라 트랜스포트 인크. 통과세포외배출용 전달 구조체 및 관련 방법
AU2021399935A1 (en) 2020-12-18 2023-06-29 Richter Gedeon Nyrt. Methods for the purification of refolded fc-peptide fusion protein
CN115894604B (zh) * 2022-12-16 2024-01-23 康日百奥生物科技(苏州)有限公司 重组蛋白澄清纯化方法

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Also Published As

Publication number Publication date
KR101741859B1 (ko) 2017-06-15
BRPI1011940B8 (pt) 2021-08-03
CA2765881A1 (en) 2011-01-13
AU2010270986B2 (en) 2014-05-22
CN102482321B (zh) 2015-06-17
US8952138B2 (en) 2015-02-10
JP2016028065A (ja) 2016-02-25
MX2011013898A (es) 2012-05-22
EP3366692A1 (en) 2018-08-29
JP6026608B2 (ja) 2016-11-16
EA020621B1 (ru) 2014-12-30
JP2012530782A (ja) 2012-12-06
CL2011003278A1 (es) 2012-11-09
BRPI1011940B1 (pt) 2021-07-20
CN102482321A (zh) 2012-05-30
EP2445923A1 (en) 2012-05-02
CA2765881C (en) 2016-08-02
IL216954A0 (en) 2012-02-29
SG176963A1 (en) 2012-02-28
WO2011005488A1 (en) 2011-01-13
IL216954A (en) 2016-09-29
BRPI1011940A2 (pt) 2016-11-22
AU2010270986A1 (en) 2012-01-19
EA201270015A1 (ru) 2012-05-30
US12269843B2 (en) 2025-04-08
US20190055281A1 (en) 2019-02-21
US20150315232A1 (en) 2015-11-05
KR20120052939A (ko) 2012-05-24
US20170145049A1 (en) 2017-05-25
US20150329586A1 (en) 2015-11-19
US9856287B2 (en) 2018-01-02
ZA201200512B (en) 2012-11-28
EP2445923B1 (en) 2018-09-05
US20100324269A1 (en) 2010-12-23

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