JP2010522561A5 - - Google Patents
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- JP2010522561A5 JP2010522561A5 JP2010500980A JP2010500980A JP2010522561A5 JP 2010522561 A5 JP2010522561 A5 JP 2010522561A5 JP 2010500980 A JP2010500980 A JP 2010500980A JP 2010500980 A JP2010500980 A JP 2010500980A JP 2010522561 A5 JP2010522561 A5 JP 2010522561A5
- Authority
- JP
- Japan
- Prior art keywords
- substrate
- protein
- binding partner
- host cell
- artificial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 claims description 91
- 102000004169 proteins and genes Human genes 0.000 claims description 91
- 210000004027 cell Anatomy 0.000 claims description 74
- 239000000758 substrate Substances 0.000 claims description 74
- 238000000034 method Methods 0.000 claims description 71
- 102000039446 nucleic acids Human genes 0.000 claims description 28
- 108020004707 nucleic acids Proteins 0.000 claims description 28
- 150000007523 nucleic acids Chemical class 0.000 claims description 28
- 230000004048 modification Effects 0.000 claims description 14
- 238000012986 modification Methods 0.000 claims description 14
- 230000001268 conjugating effect Effects 0.000 claims description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 12
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 101710167800 Capsid assembly scaffolding protein Proteins 0.000 claims description 4
- 101710130420 Probable capsid assembly scaffolding protein Proteins 0.000 claims description 4
- 101710204410 Scaffold protein Proteins 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 4
- 229960002685 biotin Drugs 0.000 claims description 4
- 235000020958 biotin Nutrition 0.000 claims description 4
- 239000011616 biotin Substances 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 239000012634 fragment Substances 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 claims description 4
- 150000002632 lipids Chemical class 0.000 claims description 4
- 229920001184 polypeptide Polymers 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 4
- 230000003362 replicative effect Effects 0.000 claims description 4
- 230000028327 secretion Effects 0.000 claims description 4
- 229920001059 synthetic polymer Polymers 0.000 claims description 4
- 230000021615 conjugation Effects 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 102000003960 Ligases Human genes 0.000 claims description 2
- 108090000364 Ligases Proteins 0.000 claims description 2
- 108010006519 Molecular Chaperones Proteins 0.000 claims description 2
- 102000005431 Molecular Chaperones Human genes 0.000 claims description 2
- 239000000427 antigen Substances 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims description 2
- 108091007433 antigens Proteins 0.000 claims description 2
- 108010053098 biotin receptor Proteins 0.000 claims description 2
- 210000002421 cell wall Anatomy 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims description 2
- 230000003993 interaction Effects 0.000 claims description 2
- 230000002452 interceptive effect Effects 0.000 claims description 2
- 230000020978 protein processing Effects 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 230000003248 secreting effect Effects 0.000 claims description 2
- 210000005253 yeast cell Anatomy 0.000 claims description 2
- 230000006287 biotinylation Effects 0.000 description 1
- 238000007413 biotinylation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US92037807P | 2007-03-26 | 2007-03-26 | |
| US92037507P | 2007-03-26 | 2007-03-26 | |
| US60/920,375 | 2007-03-26 | ||
| US60/920,378 | 2007-03-26 | ||
| US95971907P | 2007-07-16 | 2007-07-16 | |
| US60/959,719 | 2007-07-16 | ||
| US484107P | 2007-12-01 | 2007-12-01 | |
| US61/004,841 | 2007-12-01 | ||
| PCT/US2008/003978 WO2008118476A2 (en) | 2007-03-26 | 2008-03-26 | Cell surface display, screening and production of proteins of interest |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013170132A Division JP2013233157A (ja) | 2007-03-26 | 2013-08-20 | 関心対象のタンパク質の細胞表面ディスプレイ、スクリーニング、および産生 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010522561A JP2010522561A (ja) | 2010-07-08 |
| JP2010522561A5 true JP2010522561A5 (enExample) | 2012-05-10 |
| JP5754135B2 JP5754135B2 (ja) | 2015-07-29 |
Family
ID=39709282
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010500980A Expired - Fee Related JP5754135B2 (ja) | 2007-03-26 | 2008-03-26 | 関心対象のタンパク質の細胞表面ディスプレイ、スクリーニング、および産生 |
| JP2013170132A Withdrawn JP2013233157A (ja) | 2007-03-26 | 2013-08-20 | 関心対象のタンパク質の細胞表面ディスプレイ、スクリーニング、および産生 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013170132A Withdrawn JP2013233157A (ja) | 2007-03-26 | 2013-08-20 | 関心対象のタンパク質の細胞表面ディスプレイ、スクリーニング、および産生 |
Country Status (4)
| Country | Link |
|---|---|
| US (4) | US8709980B2 (enExample) |
| EP (2) | EP2617827A1 (enExample) |
| JP (2) | JP5754135B2 (enExample) |
| WO (1) | WO2008118476A2 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2617827A1 (en) | 2007-03-26 | 2013-07-24 | Celexion, LLC | Method for displaying engineered proteins on a cell surface |
| AU2009261966B2 (en) * | 2008-06-27 | 2015-04-23 | Sapphire Energy, Inc. | Induction of flocculation in photosynthetic organisms |
| US8067339B2 (en) | 2008-07-09 | 2011-11-29 | Merck Sharp & Dohme Corp. | Surface display of whole antibodies in eukaryotes |
| US20110275535A1 (en) † | 2008-12-16 | 2011-11-10 | Novartis Ag | Yeast Display Systems |
| WO2010129541A1 (en) * | 2009-05-04 | 2010-11-11 | Affomix Corporation | Emulsion selection of antibodies |
| WO2010129485A2 (en) * | 2009-05-04 | 2010-11-11 | San Diego State University Foundation | Compositions and methods for identifying enzyme and transport protein inhibitors |
| DK3636759T3 (da) * | 2009-07-17 | 2023-10-23 | Bioatla Inc | Samtidig, integreret udvælgelse og udvikling af antistof/protein-ydelse og ekspression hos produktionsværter |
| EP2516702B1 (en) | 2009-12-23 | 2018-03-21 | Affinity Biosciences Pty Ltd | Protein display |
| US9169312B2 (en) | 2010-09-21 | 2015-10-27 | San Diego State University Research Foundation | Compositions and methods for identifying enzyme and transport protein inhibitors |
| IL210093A0 (en) | 2010-12-19 | 2011-06-30 | David Helman | Membrane bound reporter molecules and their use in cell sorting |
| DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
| CA2840650C (en) | 2011-06-29 | 2019-08-20 | Affinity Biosciences Pty Ltd | Method of protein display |
| WO2013020087A2 (en) * | 2011-08-03 | 2013-02-07 | Texas Biomedical Research Insitute | Nucleic acid compositions, methods and kits for rapid pairing of affinity agents |
| US20140342932A1 (en) * | 2011-09-23 | 2014-11-20 | Merck Sharp & Dohme Corp. | Functional cell surface display of ligands for the insulin and/or insulin growth factor 1 receptor and applications thereof |
| RU2746407C1 (ru) | 2012-02-23 | 2021-04-13 | Стейдж Селл Терапеутикс Гмбх | Хроматографическое выделение клеток и других сложных биологических материалов |
| US20150322479A1 (en) * | 2012-06-20 | 2015-11-12 | Cytospan Technologies Corporation | Apparatus and method for quantification of replicative lifespan and observation of senescene |
| CN105308073B (zh) * | 2013-06-14 | 2019-08-13 | 加利福尼亚太平洋生物科学股份有限公司 | 双生物素化标签 |
| US10544449B2 (en) | 2013-06-14 | 2020-01-28 | Pacific Biosciences Of California, Inc. | Bis-biotinylation tags |
| BR112016008275A2 (pt) | 2013-10-17 | 2017-10-03 | Arsanis Biosciences Gmbh | Anticorpo com reatividade cruzada ao staphylococcus aureus, preparações farmacêuticas ou para diagnóstico compreendendo o anticorpo, anticorpo para uso médico e para diagnóstico, um ácido nucleico isolado que codifica o anticorpo, plasmideo ou cassete de expressao, e célula hospedeira compreendendo tal plasmideo ou cassete de expressao, método para produzir o anticorpo usando tal célula hospedeira, método para produzir variantes de anticorpos, um cristal formado por um monômero da hla ou lukd com dito anticorpo, um paratopo isolado do anticorpo, o epítopo isolado e uma molécula de ligação ligando especificamente ao dito epítopo, um método de triagem para identificar um ligante que se liga ao dito epítopo, um imunógeno compreendendo dito epítopo, e dito imunógeno usado para induzir uma resposta imune protetora |
| US10421958B2 (en) | 2014-02-05 | 2019-09-24 | Molecular Templates, Inc. | Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity |
| MY184699A (en) * | 2014-04-16 | 2021-04-18 | Juno Therapeutics Gmbh | Methods, kits and apparatus for expanding a population of cells |
| GB201410262D0 (en) * | 2014-06-10 | 2014-07-23 | Cambridge Entpr Ltd | Novel method |
| TWI695011B (zh) | 2014-06-18 | 2020-06-01 | 美商梅爾莎納醫療公司 | 抗ｈｅｒ２表位之單株抗體及其使用之方法 |
| ES2749716T3 (es) | 2014-10-28 | 2020-03-23 | Merck Patent Gmbh | Métodos de despliegue de proteínas que contienen dominios Fc no covalentes sobre la superficie de células y métodos de selección de las mismas |
| KR20170136637A (ko) | 2015-04-17 | 2017-12-11 | 알사니스 바이오사이언시스 게엠베하 | 항-스타필로코커스 아우레우스 항체 배합 제제 |
| MA45488A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés, kits et appareil de culture de cellules |
| CN108474002B (zh) | 2015-10-22 | 2023-05-23 | 朱诺治疗学有限公司 | 用于转导的方法、反应剂盒、反应剂和设备 |
| MA45489A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés de culture de cellules, kits et appareil associés |
| AU2016347051A1 (en) * | 2015-10-26 | 2018-05-24 | Buckman Laboratories International, Inc. | Lignocellulose polymer probes and methods |
| BR112018068461A2 (pt) | 2016-03-15 | 2019-01-22 | Mersana Therapeutics Inc | conjugado, composição farmacêutica, métodos para preparação de um conjugado e para alívio de um sintoma de um câncer. |
| WO2018041740A1 (en) | 2016-09-01 | 2018-03-08 | Bayer Pharma Aktiengesellschaft | Non-covalent display system using fimgt/dsf |
| GB201704115D0 (en) * | 2017-03-15 | 2017-04-26 | Oxford Genetics Ltd | Method of selecting for antibodies |
| SG11201909931PA (en) | 2017-04-27 | 2019-11-28 | Juno Therapeutics Gmbh | Oligomeric particle reagents and methods of use thereof |
| US11744884B2 (en) | 2017-05-15 | 2023-09-05 | University Of Maryland, Baltimore | Live Salmonella typhi vectors engineered to express heterologous outer membrane protein antigens and methods of use thereof |
| JPWO2022190733A1 (enExample) * | 2021-03-10 | 2022-09-15 | ||
| WO2023051972A1 (en) | 2021-09-29 | 2023-04-06 | Miltenyi Biotec B.V. & Co. KG | Method for the generation and selection of a producer cell |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5223409A (en) * | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| US5252466A (en) | 1989-05-19 | 1993-10-12 | Biotechnology Research And Development Corporation | Fusion proteins having a site for in vivo post-translation modification and methods of making and purifying them |
| US5747334A (en) | 1990-02-15 | 1998-05-05 | The University Of North Carolina At Chapel Hill | Random peptide library |
| WO1992006191A1 (en) * | 1990-09-28 | 1992-04-16 | Protein Engineering Corporation | Proteinaceous anti-dental plaque agents |
| US5866344A (en) | 1991-11-15 | 1999-02-02 | Board Of Regents, The University Of Texas System | Antibody selection methods using cell surface expressed libraries |
| US20030036092A1 (en) | 1991-11-15 | 2003-02-20 | Board Of Regents, The University Of Texas System | Directed evolution of enzymes and antibodies |
| US5348867A (en) | 1991-11-15 | 1994-09-20 | George Georgiou | Expression of proteins on bacterial surface |
| DK0673427T3 (da) | 1992-07-08 | 2003-07-21 | Unilever Nv | Enzymatisk proces under anvendelse af enzymer immobiliseret på cellevæggen af en eukaryotisk mikrobiel celle ved fremstilling af et fusionsprotein |
| US7166423B1 (en) * | 1992-10-21 | 2007-01-23 | Miltenyi Biotec Gmbh | Direct selection of cells by secretion product |
| DE69332884T2 (de) * | 1992-10-21 | 2003-11-20 | Stefan Miltenyi | Direkte auswahl von zellen durch ein aussheidungsprodukt |
| JP3691055B2 (ja) | 1993-02-10 | 2005-08-31 | ユニリーバー・ナームローゼ・ベンノートシャープ | 特異的結合力をもつ固定化タンパク質並びに各種プロセス・物品におけるそれらの使用 |
| WO1995004069A1 (en) * | 1993-07-30 | 1995-02-09 | Affymax Technologies N.V. | Biotinylation of proteins |
| US6300065B1 (en) * | 1996-05-31 | 2001-10-09 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US6699658B1 (en) * | 1996-05-31 | 2004-03-02 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US6696251B1 (en) | 1996-05-31 | 2004-02-24 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| DK0934526T3 (da) | 1996-10-08 | 2003-05-05 | Bisys B V U | Fremgangsmåder og midler til udvælgelse af peptider og proteiner, der har specifik affinitet til et mål |
| AU8755798A (en) * | 1997-04-04 | 1998-11-13 | Biosite Diagnostics Incorporated | Polyvalent and polyclonal libraries |
| US6057098A (en) * | 1997-04-04 | 2000-05-02 | Biosite Diagnostics, Inc. | Polyvalent display libraries |
| US6555310B1 (en) * | 1997-04-04 | 2003-04-29 | Biosite Diagnostics, Inc. | Polyclonal libraries |
| US6759243B2 (en) | 1998-01-20 | 2004-07-06 | Board Of Trustees Of The University Of Illinois | High affinity TCR proteins and methods |
| US6255075B1 (en) | 1998-10-20 | 2001-07-03 | Smithkline Beecham Corporation | Bira |
| US7223533B2 (en) * | 1999-09-10 | 2007-05-29 | Cadus Technologies, Inc. | Cell surface proteins and use thereof as indicators of activation of cellular signal transduction pathways |
| US6686168B1 (en) * | 1999-11-04 | 2004-02-03 | Zymogenetics, Inc. | Cell surface display of proteins by recombinant host cells |
| CN100365125C (zh) * | 2000-07-26 | 2008-01-30 | 韩国生命工学研究院 | 来源于酵母的新型细胞壁锚定蛋白及其基因和使用其的细胞表面表达系统 |
| US20020142355A1 (en) * | 2000-11-14 | 2002-10-03 | Baylor College Of Medicine | Methods for the in vivo biotin labeling of polypeptides |
| ATE352040T1 (de) | 2000-11-17 | 2007-02-15 | Univ Rochester | In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen |
| DE60211329T2 (de) * | 2001-01-16 | 2007-05-24 | Regeneron Pharmaceuticals, Inc. | Isolierung von sezernierte proteine exprimierenden zellen |
| ATE416192T1 (de) | 2001-04-19 | 2008-12-15 | Kansai Chem Eng | Protein, das sich an die zelloberflächenschichten bindet, und dessen verwendung |
| GB0118337D0 (en) * | 2001-07-27 | 2001-09-19 | Lonza Biologics Plc | Method for selecting antibody expressing cells |
| ES2327905T3 (es) | 2001-10-01 | 2009-11-05 | Dyax Corp. | Vectores de presentacion eucariotas multi-cadena y usos de los mismos. |
| GB0202018D0 (en) | 2002-01-29 | 2002-03-13 | Sense Proteomic Ltd | Tag and method |
| EP1367125A1 (en) | 2002-05-30 | 2003-12-03 | Centre National De La Recherche Scientifique (Cnrs) | Vectors for expression of biotinylated proteins in mammalian cells, and their use for identification of protein-nucleic acid interactions in vivo |
| US20040132133A1 (en) * | 2002-07-08 | 2004-07-08 | Invitrogen Corporation | Methods and compositions for the production, identification and purification of fusion proteins |
| US20040033603A1 (en) * | 2002-08-19 | 2004-02-19 | Lin Zhang | Biotinylation of proteins |
| FI20031663A0 (fi) | 2003-11-14 | 2003-11-14 | Jyvaeskylaen Yliopisto | Avidiinin mutantteja |
| US7795411B2 (en) * | 2006-10-05 | 2010-09-14 | Fred Hutchinson Cancer Research Center | Vectors for expressing in vivo biotinylated recombinant proteins |
| CA2666336C (en) | 2006-10-13 | 2014-01-28 | Archer Daniels Midland Company | Use of surface display proteins to display inorganic catalysts on the surface of a yeast, bacterium or virion |
| WO2008067547A2 (en) | 2006-11-30 | 2008-06-05 | Research Development Foundation | Improved immunoglobulin libraries |
| EP2617827A1 (en) | 2007-03-26 | 2013-07-24 | Celexion, LLC | Method for displaying engineered proteins on a cell surface |
| US8043830B2 (en) | 2008-02-01 | 2011-10-25 | The Regents Of The University Of California | Biotin-ligase system for secretion of biotinylated protein |
-
2008
- 2008-03-26 EP EP12154795.4A patent/EP2617827A1/en not_active Withdrawn
- 2008-03-26 US US12/056,264 patent/US8709980B2/en not_active Expired - Fee Related
- 2008-03-26 JP JP2010500980A patent/JP5754135B2/ja not_active Expired - Fee Related
- 2008-03-26 EP EP08727167.2A patent/EP2137308B1/en not_active Not-in-force
- 2008-03-26 WO PCT/US2008/003978 patent/WO2008118476A2/en not_active Ceased
-
2009
- 2009-08-24 US US12/546,583 patent/US8722586B2/en not_active Expired - Fee Related
-
2013
- 2013-08-20 JP JP2013170132A patent/JP2013233157A/ja not_active Withdrawn
-
2014
- 2014-03-26 US US14/226,114 patent/US9645146B2/en not_active Expired - Fee Related
-
2017
- 2017-03-28 US US15/471,604 patent/US20170261506A1/en not_active Abandoned
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