GB0118337D0 - Method for selecting antibody expressing cells - Google Patents
Method for selecting antibody expressing cellsInfo
- Publication number
- GB0118337D0 GB0118337D0 GBGB0118337.5A GB0118337A GB0118337D0 GB 0118337 D0 GB0118337 D0 GB 0118337D0 GB 0118337 A GB0118337 A GB 0118337A GB 0118337 D0 GB0118337 D0 GB 0118337D0
- Authority
- GB
- United Kingdom
- Prior art keywords
- expressing cells
- antibody expressing
- antibody
- selecting antibody
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
- A61K39/44—Antibodies bound to carriers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
A method for selecting high level antibody producing cells, comprising the step of attaching protein A or protein G to a cell surface, thus capturing secreted, soluble antibody. Fluorescence staining of the captured antibody by conventional methods and subsequent FACS analysis allows to select low abundance high producer single cells.
Priority Applications (12)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0118337.5A GB0118337D0 (en) | 2001-07-27 | 2001-07-27 | Method for selecting antibody expressing cells |
JP2003517592A JP4202251B2 (en) | 2001-07-27 | 2002-07-19 | Method for selecting antibody-expressing cells |
ES02762376T ES2243757T3 (en) | 2001-07-27 | 2002-07-19 | METHOD FOR SELECTING CELLS THAT EXPRESS ANTIBODIES. |
US10/484,928 US20040219611A1 (en) | 2001-07-27 | 2002-07-19 | Method for selecting antibody expressing cells |
DE60204699T DE60204699T2 (en) | 2001-07-27 | 2002-07-19 | METHOD FOR SELECTION OF CELLS EXPRESSING ANTIBODIES |
CNB028147545A CN1296710C (en) | 2001-07-27 | 2002-07-19 | Method for selecting antibody expressing cells |
PCT/EP2002/008054 WO2003012449A2 (en) | 2001-07-27 | 2002-07-19 | Method for selecting antibody expressing cells |
CA002454088A CA2454088A1 (en) | 2001-07-27 | 2002-07-19 | Method for selecting antibody expressing cells |
KR1020047001203A KR100663687B1 (en) | 2001-07-27 | 2002-07-19 | Method for selecting antibody expressing cells |
PT02762376T PT1415158E (en) | 2001-07-27 | 2002-07-19 | METHOD FOR SELECTING CELLS EXPRESSING ANTIBODIES |
AT02762376T ATE298090T1 (en) | 2001-07-27 | 2002-07-19 | METHOD FOR SELECTING CELLS THAT EXPRESS ANTIBODIES |
EP02762376A EP1415158B1 (en) | 2001-07-27 | 2002-07-19 | Method for selecting antibody expressing cells |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0118337.5A GB0118337D0 (en) | 2001-07-27 | 2001-07-27 | Method for selecting antibody expressing cells |
Publications (1)
Publication Number | Publication Date |
---|---|
GB0118337D0 true GB0118337D0 (en) | 2001-09-19 |
Family
ID=9919307
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GBGB0118337.5A Ceased GB0118337D0 (en) | 2001-07-27 | 2001-07-27 | Method for selecting antibody expressing cells |
Country Status (12)
Country | Link |
---|---|
US (1) | US20040219611A1 (en) |
EP (1) | EP1415158B1 (en) |
JP (1) | JP4202251B2 (en) |
KR (1) | KR100663687B1 (en) |
CN (1) | CN1296710C (en) |
AT (1) | ATE298090T1 (en) |
CA (1) | CA2454088A1 (en) |
DE (1) | DE60204699T2 (en) |
ES (1) | ES2243757T3 (en) |
GB (1) | GB0118337D0 (en) |
PT (1) | PT1415158E (en) |
WO (1) | WO2003012449A2 (en) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1668634A (en) * | 2002-05-22 | 2005-09-14 | 比奥根艾迪克Ma公司 | Detection of secreted polypeptides |
US20040067532A1 (en) | 2002-08-12 | 2004-04-08 | Genetastix Corporation | High throughput generation and affinity maturation of humanized antibody |
US7993864B2 (en) * | 2002-12-03 | 2011-08-09 | Ucb Pharma S.A. | Assay for identifying antibody producing cells |
WO2005019824A1 (en) * | 2003-08-20 | 2005-03-03 | Celltech R & D Limited | Methods for obtaining antibodies |
EP1797436A2 (en) * | 2004-10-08 | 2007-06-20 | Cedars-Sinai Medical Center | Biological microbeads for various flow cytometric applications |
US8945857B2 (en) * | 2005-07-01 | 2015-02-03 | John Schrader | Methods of isolating cells and generating monoclonal antibodies |
EP2137308B1 (en) * | 2007-03-26 | 2016-08-03 | Agenus Inc. | Cell surface display, screening and production of proteins of interest |
US8877688B2 (en) | 2007-09-14 | 2014-11-04 | Adimab, Llc | Rationally designed, synthetic antibody libraries and uses therefor |
EP3124497B1 (en) | 2007-09-14 | 2020-04-15 | Adimab, LLC | Rationally designed, synthetic antibody libraries and uses therefor |
EP2196804A4 (en) * | 2007-10-05 | 2010-09-15 | Kaneka Corp | Labeled peptide having activity of binding to immunoglobulin and/or immunoglobulin complex and method of detecting or assaying immunoglobulin by using the peptide |
US8637435B2 (en) * | 2007-11-16 | 2014-01-28 | Merck Sharp & Dohme Corp. | Eukaryotic cell display systems |
CA2715212A1 (en) | 2008-03-03 | 2009-09-11 | Glycofi, Inc. | Surface display of recombinant proteins in lower eukaryotes |
US8067339B2 (en) | 2008-07-09 | 2011-11-29 | Merck Sharp & Dohme Corp. | Surface display of whole antibodies in eukaryotes |
EP2390661B1 (en) * | 2010-05-02 | 2015-06-24 | Miltenyi Biotec GmbH | An anchoring/capturing means for selecting or analyzing a CHO cell according to a product secreted by the CHO cell |
AU2011279073B2 (en) | 2010-07-16 | 2016-06-09 | Adimab, Llc | Antibody libraries |
CN103185803A (en) | 2011-12-31 | 2013-07-03 | 深圳迈瑞生物医疗电子股份有限公司 | Method and kit for identifying sensitivity of antibody and clone cell strain |
US8921055B2 (en) * | 2012-10-30 | 2014-12-30 | Berkeley Lights, Inc. | Detecting cells secreting a protein of interest |
US9910038B2 (en) | 2012-11-30 | 2018-03-06 | Larix Bioscience, Llc | Cell line screening method |
WO2015161835A1 (en) * | 2014-04-20 | 2015-10-29 | New / Era / Mabs Gmbh | Biomolecule-releasing cell and selection thereof by means of a surface protein |
WO2015184626A1 (en) * | 2014-06-05 | 2015-12-10 | Wuhan Yzy Biopharma Co., Ltd | Preparation and selection of cells for producing bispecific antibodies |
CN104833625B (en) * | 2015-06-04 | 2018-02-23 | 武汉友芝友生物制药有限公司 | The early stage authentication method of expressing recombinant antibody Chinese hamster ovary celI system stability |
US9395367B1 (en) | 2016-02-29 | 2016-07-19 | Rarecyte, Inc. | Method to identify antigen-specific B cells for antibody development |
US9442113B1 (en) | 2016-02-29 | 2016-09-13 | Rarecyte, Inc. | Method to identify antigen-specific immune cells for therapeutic development |
US10222373B2 (en) | 2016-02-29 | 2019-03-05 | Rarecyte, Inc. | Method to identify antigen-specific immune cells |
CN112710833B (en) * | 2021-01-13 | 2023-01-31 | 上海交通大学 | Cell capture method based on microtubule fluidic chip |
JPWO2022190733A1 (en) * | 2021-03-10 | 2022-09-15 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5028524A (en) * | 1987-04-24 | 1991-07-02 | Takeda Chemical Industries, Ltd. | Assay for anti-pre-S antibody |
EP0805982A1 (en) * | 1995-01-25 | 1997-11-12 | Murex Diagnostics Corporation | Improvements to immunoassays |
WO1999058977A1 (en) * | 1998-05-11 | 1999-11-18 | Miltenyi Biotec Gmbh | Method of direct selection of antigen-specific t cells |
JP4022005B2 (en) * | 1998-10-22 | 2007-12-12 | わかもと製薬株式会社 | Simple antibody test method and test kit |
JP2001059845A (en) * | 1999-08-24 | 2001-03-06 | Fuji Photo Film Co Ltd | Method and element for dry analysis |
GB0002539D0 (en) * | 2000-02-03 | 2000-03-29 | Lonza Biologics Plc | Process for the discrimination of immunoglobulin G-producing cells from non-immunoglobulin G-producing cells |
US6919183B2 (en) * | 2001-01-16 | 2005-07-19 | Regeneron Pharmaceuticals, Inc. | Isolating cells expressing secreted proteins |
-
2001
- 2001-07-27 GB GBGB0118337.5A patent/GB0118337D0/en not_active Ceased
-
2002
- 2002-07-19 ES ES02762376T patent/ES2243757T3/en not_active Expired - Lifetime
- 2002-07-19 CN CNB028147545A patent/CN1296710C/en not_active Expired - Fee Related
- 2002-07-19 CA CA002454088A patent/CA2454088A1/en not_active Abandoned
- 2002-07-19 WO PCT/EP2002/008054 patent/WO2003012449A2/en active IP Right Grant
- 2002-07-19 KR KR1020047001203A patent/KR100663687B1/en not_active IP Right Cessation
- 2002-07-19 US US10/484,928 patent/US20040219611A1/en not_active Abandoned
- 2002-07-19 AT AT02762376T patent/ATE298090T1/en not_active IP Right Cessation
- 2002-07-19 JP JP2003517592A patent/JP4202251B2/en not_active Expired - Fee Related
- 2002-07-19 PT PT02762376T patent/PT1415158E/en unknown
- 2002-07-19 DE DE60204699T patent/DE60204699T2/en not_active Expired - Fee Related
- 2002-07-19 EP EP02762376A patent/EP1415158B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
KR20040021660A (en) | 2004-03-10 |
EP1415158A2 (en) | 2004-05-06 |
KR100663687B1 (en) | 2007-01-02 |
WO2003012449A3 (en) | 2003-10-23 |
EP1415158B1 (en) | 2005-06-15 |
ATE298090T1 (en) | 2005-07-15 |
ES2243757T3 (en) | 2005-12-01 |
CA2454088A1 (en) | 2003-02-13 |
PT1415158E (en) | 2005-10-31 |
CN1296710C (en) | 2007-01-24 |
DE60204699D1 (en) | 2005-07-21 |
DE60204699T2 (en) | 2006-05-11 |
WO2003012449A2 (en) | 2003-02-13 |
JP4202251B2 (en) | 2008-12-24 |
US20040219611A1 (en) | 2004-11-04 |
CN1545621A (en) | 2004-11-10 |
JP2005526950A (en) | 2005-09-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
AT | Applications terminated before publication under section 16(1) |