WO2001035072A3 - A system for cell-based screening - Google Patents

A system for cell-based screening Download PDF

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Publication number
WO2001035072A3
WO2001035072A3 PCT/US2000/030896 US0030896W WO0135072A3 WO 2001035072 A3 WO2001035072 A3 WO 2001035072A3 US 0030896 W US0030896 W US 0030896W WO 0135072 A3 WO0135072 A3 WO 0135072A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
identification
cells
mask
state
Prior art date
Application number
PCT/US2000/030896
Other languages
French (fr)
Other versions
WO2001035072A2 (en
Inventor
Richik N Ghosh
Richard Debiasio
Yih-Tai Chen
Paolo Bellutta
Kenneth Giuliano
Jefferson W Pasley
Original Assignee
Cellomics Inc
Richik N Ghosh
Richard Debiasio
Chen Yih Tai
Paolo Bellutta
Kenneth Giuliano
Jefferson W Pasley
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc, Richik N Ghosh, Richard Debiasio, Chen Yih Tai, Paolo Bellutta, Kenneth Giuliano, Jefferson W Pasley filed Critical Cellomics Inc
Priority to AU16564/01A priority Critical patent/AU1656401A/en
Publication of WO2001035072A2 publication Critical patent/WO2001035072A2/en
Publication of WO2001035072A3 publication Critical patent/WO2001035072A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physiology (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides methods, computer readable storage medium, and kits for cell state identification in cells, where the method includes providing arrays of cells that possess luminescently labelled cell identification and cell state reporter molecules that have distinguishable luminescent emission spectra; imaging the cells to obtain luminescent signals from the cell identification and the cell state reporter molecules; converting the luminescent signals into digital data to create a mask of the cell identification reporter molecule and the cell state reporter molecules; and determining the intensity of the cell state reporter molecule mask that co-localizes with the cell identification reporter molecule mask to identify the cell as being in a particular physiological state.
PCT/US2000/030896 1999-11-09 2000-11-09 A system for cell-based screening WO2001035072A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU16564/01A AU1656401A (en) 1999-11-09 2000-11-09 A system for cell-based screening

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US16435399P 1999-11-09 1999-11-09
US60/164,353 1999-11-09
US17650400P 2000-01-18 2000-01-18
US60/176,504 2000-01-18

Publications (2)

Publication Number Publication Date
WO2001035072A2 WO2001035072A2 (en) 2001-05-17
WO2001035072A3 true WO2001035072A3 (en) 2001-11-22

Family

ID=26860473

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/030896 WO2001035072A2 (en) 1999-11-09 2000-11-09 A system for cell-based screening

Country Status (2)

Country Link
AU (1) AU1656401A (en)
WO (1) WO2001035072A2 (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US20030054543A1 (en) * 1997-06-16 2003-03-20 Lafferty William Michael Device for moving a selected station of a holding plate to a predetermined location for interaction with a probe
US20020102598A1 (en) * 1997-06-16 2002-08-01 Lafferty William Michael Positioning system for moving a selected station of a holding plate to a predetermined location for interaction with a probe
US7151847B2 (en) 2001-02-20 2006-12-19 Cytokinetics, Inc. Image analysis of the golgi complex
US6986993B1 (en) 1999-08-05 2006-01-17 Cellomics, Inc. System for cell-based screening
WO2001042786A2 (en) 1999-12-09 2001-06-14 Cellomics, Inc. System for cell based screening : cell spreading
AU2002230415A1 (en) 2000-11-17 2002-05-27 Universal Imaging Corporation Rapidly changing dichroic beamsplitter
US7218764B2 (en) 2000-12-04 2007-05-15 Cytokinetics, Inc. Ploidy classification method
DE60103428T2 (en) 2000-12-22 2005-06-02 Cellomics, Inc. Identification of cells during kinetic trials
US7016787B2 (en) 2001-02-20 2006-03-21 Cytokinetics, Inc. Characterizing biological stimuli by response curves
US6956961B2 (en) 2001-02-20 2005-10-18 Cytokinetics, Inc. Extracting shape information contained in cell images
JP2003294736A (en) * 2002-04-01 2003-10-15 Japan Science & Technology Corp Screening method using fluorescent protein fusion probe
US20050214734A1 (en) * 2002-04-01 2005-09-29 Seishi Kato Cell population provided with identification codes and method of screening cell population
JP4033697B2 (en) * 2002-04-01 2008-01-16 独立行政法人科学技術振興機構 Cell chip preparation method and target protein screening method
US6658143B2 (en) * 2002-04-29 2003-12-02 Amersham Biosciences Corp. Ray-based image analysis for biological specimens
US20050014217A1 (en) * 2003-07-18 2005-01-20 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
EP1646926A2 (en) 2003-07-18 2006-04-19 Cytokinetics, Inc. Characterizing biological stimuli by response curves
US7235353B2 (en) 2003-07-18 2007-06-26 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
US7323318B2 (en) 2004-07-15 2008-01-29 Cytokinetics, Inc. Assay for distinguishing live and dead cells
CA2652562C (en) 2006-05-17 2015-05-12 Cellumen, Inc. Method for automated tissue analysis
US9001200B2 (en) 2010-01-12 2015-04-07 Bio-Rad Laboratories, Inc. Cell characterization using multiple focus planes
ES2739623T3 (en) 2011-03-17 2020-02-03 Cernostics Inc Systems and compositions for diagnosing Barrett's esophagus and methods for using them
GB201121959D0 (en) * 2011-12-21 2012-02-01 Univ Leeds Assay of functional cell viability
US11749569B2 (en) * 2020-05-06 2023-09-05 Taiwan Semiconductor Manufacturing Company, Ltd. Method for non-destructive inspection of cell etch redeposition
DE102020125929A1 (en) * 2020-05-06 2021-11-11 Taiwan Semiconductor Manufacturing Co., Ltd. PROCEDURE FOR NON-DESTRUCTIVE VERIFICATION OF PARASITAR ETCH DEPOSIT ON CELLS
WO2024097388A1 (en) * 2022-11-03 2024-05-10 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for monitoring fatty acid metabolism

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993016442A1 (en) * 1992-02-18 1993-08-19 Neopath, Inc. Method for identifying objects using data processing techniques
US5314805A (en) * 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
US5764792A (en) * 1996-01-19 1998-06-09 Oncor, Inc. Method and apparatus for processing images
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO1999045357A2 (en) * 1998-03-02 1999-09-10 Trustees Of Tufts College Biosensor array comprising cell populations confined to microcavities
WO2000050872A2 (en) * 1999-02-26 2000-08-31 Cellomics, Inc. A system for cell-based screening

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5314805A (en) * 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
WO1993016442A1 (en) * 1992-02-18 1993-08-19 Neopath, Inc. Method for identifying objects using data processing techniques
US5764792A (en) * 1996-01-19 1998-06-09 Oncor, Inc. Method and apparatus for processing images
WO1997045730A1 (en) * 1996-05-30 1997-12-04 Biodx Miniaturized cell array methods and apparatus for cell-based screening
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
WO1999045357A2 (en) * 1998-03-02 1999-09-10 Trustees Of Tufts College Biosensor array comprising cell populations confined to microcavities
WO2000050872A2 (en) * 1999-02-26 2000-08-31 Cellomics, Inc. A system for cell-based screening

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
POOT M ET AL: "FLOW CYTOMETRIC ANALYSIS OF CELL CYCLE-DEPENDENT CHANGES IN CELL THIOL LEVEL BY COMBINING A NEW LASER DYE WITH HOECHST 33342", CYTOMETRY, vol. 12, no. 2, 1991, pages 184 - 187, XP001006094, ISSN: 0196-4763 *
SCHAPER MICHELLE M ET AL: "Computer programs for calculation of median effective dose (LD-50 or ED-50) using the method of moving average interpolation.", ARCHIVES OF TOXICOLOGY, vol. 68, no. 5, 1994, pages 332 - 337, XP001006095, ISSN: 0340-5761 *

Also Published As

Publication number Publication date
WO2001035072A2 (en) 2001-05-17
AU1656401A (en) 2001-06-06

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