ATE352040T1 - In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen - Google Patents

In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen

Info

Publication number
ATE352040T1
ATE352040T1 AT01274137T AT01274137T ATE352040T1 AT E352040 T1 ATE352040 T1 AT E352040T1 AT 01274137 T AT01274137 T AT 01274137T AT 01274137 T AT01274137 T AT 01274137T AT E352040 T1 ATE352040 T1 AT E352040T1
Authority
AT
Austria
Prior art keywords
immunoglobulin molecules
producing
eukaryotic cells
antigen
vitro method
Prior art date
Application number
AT01274137T
Other languages
English (en)
Inventor
Maurice Zauderer
Ernest S Smith
Original Assignee
Univ Rochester
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rochester filed Critical Univ Rochester
Application granted granted Critical
Publication of ATE352040T1 publication Critical patent/ATE352040T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/023—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a poxvirus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT01274137T 2000-11-17 2001-11-14 In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen ATE352040T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US24926800P 2000-11-17 2000-11-17
US26206701P 2001-01-18 2001-01-18
US27142401P 2001-02-27 2001-02-27
US29808701P 2001-06-15 2001-06-15

Publications (1)

Publication Number Publication Date
ATE352040T1 true ATE352040T1 (de) 2007-02-15

Family

ID=27500312

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01274137T ATE352040T1 (de) 2000-11-17 2001-11-14 In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen

Country Status (9)

Country Link
US (2) US7858559B2 (de)
EP (1) EP1340088B1 (de)
JP (1) JP4368196B2 (de)
CN (1) CN1306272C (de)
AT (1) ATE352040T1 (de)
AU (1) AU2001297872B2 (de)
CA (1) CA2429544C (de)
DE (1) DE60126130T2 (de)
WO (1) WO2002102855A2 (de)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696251B1 (en) * 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6759243B2 (en) * 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
EP1268763A2 (de) * 2000-03-28 2003-01-02 University Of Rochester Verfahren zur herstellung einer bibliothek und zur selektion von polynukleotiden
CN101643732A (zh) * 2000-04-04 2010-02-10 罗切斯特大学 在乳腺癌和膀胱癌中差异表达的基因及编码的多肽
US20050196755A1 (en) * 2000-11-17 2005-09-08 Maurice Zauderer In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells
JP4368196B2 (ja) * 2000-11-17 2009-11-18 ユニバーシティー オブ ロチェスター 真核細胞において免疫グロブリン分子を製造および同定するインビトロにおける方法
EP1830190A3 (de) * 2000-11-17 2008-07-09 University Of Rochester In-vitro-Verfahren zur Herstellung und Identifizierung von Immunoglobulin-Molekülen in eukaryontischen Zellen
WO2003104428A2 (en) 2002-01-21 2003-12-18 Vaccinex, Inc. Gene differentially expressed in breast and bladder cancer and encoded polypeptides
WO2003089614A2 (en) * 2002-04-18 2003-10-30 Genencor International, Inc. Production of functional antibodies in filamentous fungi
GB0221940D0 (en) * 2002-09-20 2002-10-30 Athena Biodetection Aps Antibody and screening method
JP4477579B2 (ja) * 2003-01-21 2010-06-09 中外製薬株式会社 抗体の軽鎖スクリーニング方法
EP2902491A1 (de) 2003-03-19 2015-08-05 Biogen MA Inc. NOGO Rezeptor bindendes Protein
CN1542132A (zh) * 2003-04-30 2004-11-03 上海新霁生物科技有限公司 高效表达治疗肿瘤并含有人恒定区全抗体基因的重组病毒及其用途
WO2005055936A2 (en) * 2003-12-04 2005-06-23 Vaccinex, Inc. Methods of killing tumor cells by targeting internal antigens exposed on apoptotic tumor cells
AU2004309373A1 (en) * 2003-12-22 2005-07-14 Amgen Inc. Methods for identifying functional antibodies
US20050266425A1 (en) * 2003-12-31 2005-12-01 Vaccinex, Inc. Methods for producing and identifying multispecific antibodies
EP1776136B1 (de) 2004-06-24 2012-10-03 Biogen Idec MA Inc. Behandlung von entmyelinisierenden erkrankungen
EP1789070B1 (de) 2004-08-03 2012-10-24 Biogen Idec MA Inc. Taj in der neuronalen funktion
EP1885186B1 (de) * 2005-06-01 2015-09-02 California Institute Of Technology Verfahren zur gezielten genabgabe über virale vektoren
AU2006269458B2 (en) 2005-07-08 2012-11-08 Biogen Ma Inc. Sp35 antibodies and uses thereof
JP2009511067A (ja) * 2005-10-14 2009-03-19 メディミューン，エルエルシー 抗体ライブラリーの細胞提示
AU2006311828B2 (en) 2005-11-04 2013-07-11 Biogen Ma Inc. Methods for promoting neurite outgrowth and survival of dopaminergic neurons
NZ569428A (en) 2005-12-02 2012-11-30 Biogen Idec Inc Treatment of conditions involving demyelination with a Sp35 antagonist
JP5829373B2 (ja) 2006-01-27 2015-12-09 バイオジェン・エムエイ・インコーポレイテッドＢｉｏｇｅｎ ＭＡ Ｉｎｃ． Ｎｏｇｏレセプターアンタゴニスト
EA200802061A1 (ru) * 2006-03-28 2009-04-28 Байоджен Айдек Эмэй Инк. Антитело или его фрагмент, специфично связывающееся с рецептором 1 инсулиноподобного фактора роста (igf-r1) (варианты), композиция на его основе, полинуклеотид, кодирующий вариабельную область антитела (варианты), содержащие полинуклеотид композиция (варианты) и вектор, содержащая вектор клетка-хозяин (варианты), способ продуцирования антитела или его фрагмента (варианты) и способ лечения гиперпролиферативного заболевания у животного организма
US20080305111A1 (en) * 2006-06-22 2008-12-11 Vaccinex, Inc. Anti-C35 antibodies for treating cancer
BRPI0715115A2 (pt) * 2006-08-03 2013-06-04 Vaccinex Inc anticorpo monoclonal isolado, molÉcula de Ácido nucleico isolada, vetor de expressço, cÉlula hospedeira, mÉtodos para tratar uma doenÇa, e para produzir um anticorpo monoclonal isolado, uso do anticorpo monoclonal isolado, e, composiÇço farmacÊutica
US9382327B2 (en) 2006-10-10 2016-07-05 Vaccinex, Inc. Anti-CD20 antibodies and methods of use
EP1921142A1 (de) 2006-11-07 2008-05-14 Cytos Biotechnology AG Auswahl von menschlichen monoklonalen Antikörpern unter Verwendung von eukaryontischem Zell-Display
EP2099826B1 (de) 2007-01-05 2013-10-16 University Of Zurich Anti-beta-amyloid-antikörper und verwendung davon
US8128926B2 (en) 2007-01-09 2012-03-06 Biogen Idec Ma Inc. Sp35 antibodies and uses thereof
ES2464815T3 (es) 2007-01-09 2014-06-04 Biogen Idec Ma Inc. Anticuerpos SP35 y usos de éstos
EP2137308B1 (de) 2007-03-26 2016-08-03 Agenus Inc. Zelloberflächenpräsentation, screening und produktion von interessierenden proteinen
US7807168B2 (en) * 2007-04-10 2010-10-05 Vaccinex, Inc. Selection of human TNFα specific antibodies
WO2009032145A1 (en) * 2007-08-28 2009-03-12 Biogen Idec Ma Inc. Anti-igf-1r antibodies and uses thereof
US20090130105A1 (en) * 2007-08-28 2009-05-21 Biogen Idec Ma Inc. Compositions that bind multiple epitopes of igf-1r
US7947495B2 (en) 2007-11-01 2011-05-24 Abbott Biotherapeutics Corp. Immunoglobulin display vectors
ES2566963T3 (es) 2007-11-21 2016-04-18 Oregon Health & Science University Anticuerpos monoclonales anti-factor XI y métodos de uso de los mismos
KR101642846B1 (ko) 2007-12-26 2016-07-26 백시넥스 인코포레이티드 항-ｃ35 항체 병용 치료 및 방법
EP2098536A1 (de) * 2008-03-05 2009-09-09 4-Antibody AG Isolation und Identifikation antigen- oder ligandenspezifischer Bindungsproteine
CN102065895A (zh) * 2008-04-11 2011-05-18 比奥根艾迪克Ma公司 抗-igf-1r抗体和其它化合物的治疗联合
DK2982695T3 (da) * 2008-07-09 2019-05-13 Biogen Ma Inc Sammensætninger, der omfatter antistoffer mod lingo eller fragmenter deraf
EP3521309A1 (de) 2008-12-19 2019-08-07 Biogen International Neuroscience GmbH Menschliche anti-alpha-synuclein-antikörper
US20120003235A1 (en) 2008-12-31 2012-01-05 Biogen Idec Ma Inc. Anti-lymphotoxin antibodies
JP2012521216A (ja) 2009-03-24 2012-09-13 テバ バイオファーマスーティカルズ ユーエスエー，インコーポレーテッド Ｌｉｇｈｔに対するヒト化抗体およびその使用
EP3320920A1 (de) 2009-05-08 2018-05-16 Vaccinex, Inc. Anti-cd100-antikörper und verfahren zur verwendung davon
NZ601743A (en) * 2010-02-12 2014-11-28 Oncomed Pharm Inc Methods for identifying and isolating cells expressing a polypeptide
EP2558587A4 (de) * 2010-04-12 2015-05-06 Sorrento Therapeutics Inc Verfahren zur anzeige von antikörpern
US20130236905A1 (en) * 2010-05-18 2013-09-12 Christopher Marshall Assay for identifying antigens that activate b cell receptors comprising neutralizing antibodies
JP6097690B2 (ja) 2010-09-02 2017-03-15 ヴァクシネックス， インコーポレイテッド 抗ｃｘｃｌ１３抗体およびそれを用いる方法
US8940272B2 (en) 2010-10-11 2015-01-27 University Of Zurich Human anti-tau antibodies
US9365846B2 (en) * 2010-12-01 2016-06-14 Merck Sharp & Dohme Corp. Surface, anchored Fc-bait antibody display system
US10106598B2 (en) 2010-12-01 2018-10-23 Merck Sharp & Dohme Corp. Surface, anchored Fc-bait antibody display system
EP2651979B1 (de) 2010-12-17 2019-08-28 Neurimmune Holding AG Humane anti-sod-1 antikörper
CN103620405B (zh) * 2010-12-31 2016-05-25 生物蛋白有限公司 全面单克隆抗体产生
CA2838211C (en) 2011-06-10 2023-08-01 Medimmmune Limited Anti-pseudomonas psl binding molecules and uses thereof
MX357193B (es) 2011-06-23 2018-06-29 Univ Zuerich Moleculas de union anti-alfa sinucleina.
HUE050985T2 (hu) 2011-11-07 2021-01-28 Medimmune Ltd Pseudomonas PSL és PCRV elleni kötõmolekulát alkalmazó kombinációs terápiák
HK1200849A1 (en) 2011-12-22 2015-08-14 F. Hoffmann-La Roche Ag Full length antibody display system for eukaryotic cells and its use
US9708601B2 (en) 2012-04-26 2017-07-18 Vaccinex, Inc. Fusion proteins to facilitate selection of cells infected with specific immunoglobulin gene recombinant vaccinia virus
NZ702178A (en) 2012-05-14 2017-01-27 Biogen Ma Inc Lingo-2 antagonists for treatment of conditions involving motor neurons
BR112015014751A8 (pt) 2012-12-21 2018-01-16 Biogen Int Neuroscience Gmbh anti-corpos anti-tau humanos e fragmento de ligação a tau dos mesmo, seu uso na preparação de um medicamento, bem como polipeptídeos codificando os mesmos.
WO2014102399A1 (en) 2012-12-31 2014-07-03 Neurimmune Holding Ag Recombinant human antibodies for therapy and prevention of polyomavirus-related diseases
NZ710422A (en) 2013-01-24 2016-11-25 Scripps Korea Antibody Inst Protein combination-based fv library, and preparation method therefor
CN103214580B (zh) * 2013-03-07 2014-12-17 中国人民解放军军事医学科学院基础医学研究所 一种抗Her2免疫细胞因子及其应用
GB201407852D0 (en) 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
EA201790165A1 (ru) 2014-07-29 2017-07-31 Нейриммьюн Холдинг Аг Происходящие от человека антитела к гентингтину (htt) и их применение
AU2015326911C1 (en) 2014-09-30 2025-12-18 Neurimmune Holding Ag Human-derived anti-dipeptide repeats (DPRs) antibody
WO2016112270A1 (en) 2015-01-08 2016-07-14 Biogen Ma Inc. Lingo-1 antagonists and uses for treatment of demyelinating disorders
MY190209A (en) 2015-03-31 2022-04-05 Medimmune Ltd A novel il33 form, mutated forms of il33, antibodies, assays and methods of using the same
WO2017048902A1 (en) 2015-09-15 2017-03-23 Board Of Regents, The University Of Texas System T-cell receptor (tcr)-binding antibodies and uses thereof
US11072652B2 (en) 2016-03-10 2021-07-27 Viela Bio, Inc. ILT7 binding molecules and methods of using the same
EP3445397B1 (de) 2016-04-22 2022-11-09 Vaccinex, Inc. Integrale membranproteinanzeige auf extrazellulären umhüllten virionen des pockenvirus
JP2018035137A (ja) 2016-07-13 2018-03-08 マブイミューン ダイアグノスティックス エイジーＭａｂｉｍｍｕｎｅ Ｄｉａｇｎｏｓｔｉｃｓ Ａｇ 新規な抗線維芽細胞活性化タンパク質（ｆａｐ）結合薬剤およびその使用
CA3032512C (en) 2016-08-02 2025-05-06 Vaccinex, Inc. IMPROVED PROCESSES FOR PRODUCING POLYNUCLEOTIDE LIBRARIES IN VACCINE VIRUS/EUKARYOTIC CELLS
CN106905415B (zh) * 2017-02-23 2021-04-20 新疆军区总医院 与耐药宫颈癌癌细胞系Hela细胞膜表面分子结合的多肽
US11427634B2 (en) 2017-05-05 2022-08-30 Vaccinex, Inc. Human anti-semaphorin 4D antibody
CN107418913B (zh) * 2017-06-14 2020-12-22 湖南省农业生物技术研究中心 一种转化污染土壤中重金属镉的微生物菌剂的应用
US20220023342A1 (en) 2018-11-30 2022-01-27 Institut Gustave Roussy Anti-neuropilin-1 and anti-programmed cell death-1 combination therapy for treating cancer
KR20210126078A (ko) 2019-02-13 2021-10-19 더 브리검 앤드 우먼즈 하스피털, 인크. 항-말초 림프절 어드레신 항체 및 그의 용도
WO2020232165A1 (en) * 2019-05-14 2020-11-19 Qlb Biotherapeutics Bispecific anti-cd3 x cd20 antibodies and uses thereof
EP4545566A3 (de) 2020-02-28 2025-10-08 The Brigham and Women's Hospital Inc. Selektive modulation der signalisierung der transformierenden wachstumsfaktor-beta-superfamilie über multispezifische antikörper
IL297578A (en) 2020-05-06 2022-12-01 Vaccinex Inc Integral membrane protein display on extracellular enveloped poxviruses
CR20230533A (es) 2021-04-19 2024-04-05 Medimmune Ltd Un fab anti-tslp con estabilidad mejorada
IL317110A (en) * 2022-06-10 2025-01-01 Vaccinex Inc Methods for selecting antibodies specific for complex membrane antigens
WO2024038187A1 (en) 2022-08-19 2024-02-22 Medimmune Limited Assay for detection of il-33
WO2025104243A1 (en) 2023-11-15 2025-05-22 Neurimmune Ag Anti-transthyretin antibody, compositions comprising said antibody and methods for treating or preventing transthyretin-mediated amyloidosis

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4603112A (en) * 1981-12-24 1986-07-29 Health Research, Incorporated Modified vaccinia virus
US5110587A (en) * 1981-12-24 1992-05-05 Health Research, Incorporated Immunogenic composition comprising synthetically modified vaccinia virus
US4769330A (en) * 1981-12-24 1988-09-06 Health Research, Incorporated Modified vaccinia virus and methods for making and using the same
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
DE3382317D1 (de) * 1982-03-15 1991-07-25 Schering Corp Hybride dns, damit hergestellte bindungszusammensetzung und verfahren dafuer.
US7045313B1 (en) * 1982-11-30 2006-05-16 The United States Of America As Represented By The Department Of Health And Human Services Recombinant vaccinia virus containing a chimeric gene having foreign DNA flanked by vaccinia regulatory DNA
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) * 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
JPS6147500A (ja) 1984-08-15 1986-03-07 Res Dev Corp Of Japan キメラモノクロ−ナル抗体及びその製造法
US5807715A (en) * 1984-08-27 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods and transformed mammalian lymphocyte cells for producing functional antigen-binding protein including chimeric immunoglobulin
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
DK171161B1 (da) 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
JPS63501765A (ja) 1985-11-01 1988-07-21 インタ−ナショナル、ジェネティック、エンジニアリング　インコ−ポレ−テッド 抗体遺伝子のモジュ−ル組立体、それにより産生された抗体及び用途
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5225539A (en) * 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
JPS63152984A (ja) 1986-08-18 1988-06-25 Wakunaga Pharmaceut Co Ltd 抗緑膿菌ヒト型抗体のｌ鎖をコ−ドするｄｎａ
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
US4946786A (en) * 1987-01-14 1990-08-07 President And Fellows Of Harvard College T7 DNA polymerase
WO1988006630A1 (en) 1987-03-02 1988-09-07 Genex Corporation Method for the preparation of binding molecules
CA1341235C (en) 1987-07-24 2001-05-22 Randy R. Robinson Modular assembly of antibody genes, antibodies prepared thereby and use
AU2318288A (en) 1987-08-07 1989-03-09 Genelabs Incorporated Coincidence cloning method and library
US5965405A (en) * 1988-04-16 1999-10-12 Celltech Limited Method for producing Fv fragments in eukaryotic cells
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
FI903489A0 (fi) 1988-11-11 1990-07-10 Medical Res Council Ligander med en enda sektion, receptorer innehaollande naemnda ligander, foerfaranden foer deras framstaellning samt anvaendning av liganderna och receptorerna.
US6291159B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
CA2016841C (en) 1989-05-16 1999-09-21 William D. Huse A method for producing polymers having a preselected activity
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US6291158B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertoire
US6291160B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
FI915411A0 (fi) 1989-05-16 1991-11-15 Scripps Research Inst Samexpression av heteromeriska receptorer.
US6291161B1 (en) * 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5959177A (en) * 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US5780225A (en) * 1990-01-12 1998-07-14 Stratagene Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
WO1991010737A1 (en) * 1990-01-11 1991-07-25 Molecular Affinities Corporation Production of antibodies using gene libraries
JPH06508511A (ja) 1990-07-10 1994-09-29 ケンブリッジ　アンティボディー　テクノロジー　リミティド 特異的な結合ペアーの構成員の製造方法
US5650150A (en) * 1990-11-09 1997-07-22 Gillies; Stephen D. Recombinant antibody cytokine fusion proteins
US5962255A (en) * 1992-03-24 1999-10-05 Cambridge Antibody Technology Limited Methods for producing recombinant vectors
US5871907A (en) * 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
GB9114468D0 (en) * 1991-07-04 1991-08-21 Wellcome Found Antibody production
WO1993003151A1 (en) * 1991-08-10 1993-02-18 Medical Research Council Treatment of cell populations
US5445953A (en) * 1991-08-26 1995-08-29 Immuno Aktiengesellschaft Direct molecular cloning of a modified poxvirus genome
ES2136092T3 (es) * 1991-09-23 1999-11-16 Medical Res Council Procedimientos para la produccion de anticuerpos humanizados.
US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
ATE463573T1 (de) * 1991-12-02 2010-04-15 Medimmune Ltd Herstellung von autoantikörpern auf phagenoberflächen ausgehend von antikörpersegmentbibliotheken
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
EP1498427B1 (de) * 1992-08-21 2009-12-16 Vrije Universiteit Brussel Immunoglobuline ohne leichte Ketten
US6005079A (en) * 1992-08-21 1999-12-21 Vrije Universiteit Brussels Immunoglobulins devoid of light chains
AU689755B2 (en) * 1992-09-22 1998-04-09 Biofocus Discovery Limited Recombinant viruses displaying a nonviral polypeptide on their external surface
DK0698097T3 (da) 1993-04-29 2001-10-08 Unilever Nv Produktion af antistoffer eller (funktionaliserede) fragmenter deraf afledt af Camelidae-immunoglobuliner med tung kæde
CN1045820C (zh) * 1993-11-18 1999-10-20 重庆市肿瘤研究所 一种用于测定人ⅲ型前胶原的试剂盒及其测定方法
DE69531148T2 (de) * 1994-01-31 2004-04-29 Trustees Of Boston University, Boston Bibliotheken aus polyklonalen antikörpern
FR2715940B1 (fr) * 1994-02-10 1996-04-26 Pasteur Merieux Serums Vacc Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus.
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
ATE298370T1 (de) * 1994-04-29 2005-07-15 Baxter Healthcare Sa Rekombinante poxviren mit fremdenpolynukleotiden in wichtigen regionen
US6010861A (en) * 1994-08-03 2000-01-04 Dgi Biotechnologies, Llc Target specific screens and their use for discovering small organic molecular pharmacophores
US6130364A (en) * 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
EP0739981A1 (de) 1995-04-25 1996-10-30 Vrije Universiteit Brussel Variable Fragmente von Immunglobulinen-Verwendung zur therapeutischen oder veterinären Zwecken
WO1997008320A1 (en) 1995-08-18 1997-03-06 Morphosys Gesellschaft Für Proteinoptimierung Mbh Protein/(poly)peptide libraries
US5916771A (en) * 1996-10-11 1999-06-29 Abgenix, Inc. Production of a multimeric protein by cell fusion method
WO1998047343A2 (en) * 1997-04-04 1998-10-29 Biosite Diagnostics, Inc. Antibodies or binding protein libraries displayed on phage, cells, or other replicatable genetic packages
US6057098A (en) * 1997-04-04 2000-05-02 Biosite Diagnostics, Inc. Polyvalent display libraries
US6872518B2 (en) * 1997-09-22 2005-03-29 University Of Rochester Methods for selecting polynucleotides encoding T cell epitopes
GB9722131D0 (en) * 1997-10-20 1997-12-17 Medical Res Council Method
AU749042B2 (en) 1997-12-05 2002-06-20 Intercell Biomedizinische Forschungs- Und Entwicklungs Gmbh Method and cells to screen combinatorial libraries
BR9907241A (pt) 1998-01-26 2000-10-17 Unilever Nv Biblioteca de expressão, processo para preparar a mesma, uso de uma fonte não imunizada de sequências de ácido nucleico, e, processos para preparar fragmentos de anticorpos e, para preparar um anticorpo
EP0954978B1 (de) 1998-03-12 2011-11-30 VHsquared Limited Produkten die inaktivierte Hefen oder Schimmel enthalten, die auf ihrer Aussenoberfläche aktive Antikörper haben
WO2000028016A1 (en) * 1998-11-10 2000-05-18 University Of Rochester T cells specific for target antigens and methods and vaccines based thereon
WO2000043507A1 (en) 1999-01-19 2000-07-27 Unilever Plc Method for producing antibody fragments
JP2003502024A (ja) 1999-05-14 2003-01-21 メディカル リサーチ カウンシル タンパク質骨格および単量体ポリペプチドを多量体化するためのその使用
WO2001018058A2 (en) 1999-09-07 2001-03-15 Viventia Biotech Inc. Enhanced phage display library of human vh fragments and methods for producing same
CN1301772A (zh) * 1999-12-30 2001-07-04 中国科学院生物物理研究所 特异性识别发育调控蛋白qBrn－2的抗体及其制备方法和用途
US20030194696A1 (en) * 2000-03-28 2003-10-16 University Of Rochester Methods of producing a library and methods of selecting polynucleotides of interest
EP1268763A2 (de) * 2000-03-28 2003-01-02 University Of Rochester Verfahren zur herstellung einer bibliothek und zur selektion von polynukleotiden
US6410246B1 (en) * 2000-06-23 2002-06-25 Genetastix Corporation Highly diverse library of yeast expression vectors
AU2001278872A1 (en) * 2000-07-06 2002-01-21 Genvec, Inc. Method of identifying a binding partner of a gene product
US20050196755A1 (en) * 2000-11-17 2005-09-08 Maurice Zauderer In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells
JP4368196B2 (ja) * 2000-11-17 2009-11-18 ユニバーシティー オブ ロチェスター 真核細胞において免疫グロブリン分子を製造および同定するインビトロにおける方法
AU2002338446A1 (en) * 2001-01-23 2002-11-05 University Of Rochester Medical Center Methods of producing or identifying intrabodies in eukaryotic cells
WO2002062822A2 (en) * 2001-02-02 2002-08-15 University Of Rochester Methods of identifying regulator molecules
DE60232672D1 (de) * 2001-10-01 2009-07-30 Dyax Corp Mehrkettige eukaryontische display-vektoren und deren verwendungen
WO2004037993A2 (en) 2002-10-22 2004-05-06 University Of Rochester Methods of producing a library and methods of selecting polynucleotides of interest
US20050266425A1 (en) * 2003-12-31 2005-12-01 Vaccinex, Inc. Methods for producing and identifying multispecific antibodies

Also Published As

Publication number Publication date
CN1306272C (zh) 2007-03-21
CA2429544A1 (en) 2002-12-27
JP4368196B2 (ja) 2009-11-18
EP1340088A2 (de) 2003-09-03
US8741810B2 (en) 2014-06-03
WO2002102855A2 (en) 2002-12-27
AU2001297872B2 (en) 2006-11-09
US20020123057A1 (en) 2002-09-05
WO2002102855A3 (en) 2003-06-26
EP1340088B1 (de) 2007-01-17
JP2004532645A (ja) 2004-10-28
CA2429544C (en) 2010-10-19
US20080167193A1 (en) 2008-07-10
DE60126130D1 (de) 2007-03-08
CN1527944A (zh) 2004-09-08
US7858559B2 (en) 2010-12-28
DE60126130T2 (de) 2007-10-18

Similar Documents

Publication Publication Date Title
ATE352040T1 (de) In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen
ATE407138T1 (de) Verfahren zur herstellung interferierender rna- moleküle in säugetierzellen und therapeutische anwendungen solcher moleküle
EA200400526A1 (ru) Агенты, специфически связывающиеся с человеческим ангиопоэтином-2
MXPA03009785A (es) Anticuerpo de una sola cadena pesada de vhh y metodo para su preparacion en un mamifero.
IL183370A0 (en) Monoclonal antibody production by ebv transformation of b cells
DE602004025805D1 (de) Verfahren zur herstellung von antikörpern
ATE506374T1 (de) Exprimierungskontrolle unter verwendung von variabelen intergenischen sequenzen
DE60331741D1 (de) Verfahren und zusammensetzungen zur herstellung und steuerung des effektorprofils von t-zellen durch gleichzeitiges beladen und aktivieren ausgewählter untergruppen von antigen-präsentierenden zellen
DE68921364D1 (de) Verfahren zur Herstellung von Proteinen mittels rekombinanter DNA.
CY1106821T1 (el) Μεθοδος για την καλλιεργεια υποκαταστατου χονδρου και βιολογικη μητρα, παραγομενη συμφωνα με τη μεθοδο αυτη
DE69534595D1 (de) Methode zur proliferation und differenzierung von b-zellen und verwendungen dafür
ATE468390T1 (de) System und verfahren zur produktion von antikörpern in der pflanzenzellkultur
DE60335191D1 (de) Verfahren zur förderung der homologen rekombination somatischer zellen und verfahren zur konstruktion eines spezifischen antikörpers
BRPI0411471A (pt) método para produzir um não-trançado fibroso e não-trançado fibroso
DE69718332D1 (de) ZnS-PHOTOKATALYSATOR, SEINE HERSTELLUNG UND VERFAHREN ZUR PRODUKTION VON WASSERSTOFF DURCH VERWENDUNG DESSELBEN
ATE270319T1 (de) Benzol-umwandlung in einem verbesserten verfahren zur ausreichung von kohlenwasserstoffen
DE60139993D1 (de) Chondroitin synthasegen und verfahren zur dessen herstellung und anwendung
ATE437906T1 (de) Verfahren zur herstellung von pfropfpolymerisaten
UY28558A1 (es) Proteína
AU2003226127A1 (en) Antibody libraries
EP1830190A3 (de) In-vitro-Verfahren zur Herstellung und Identifizierung von Immunoglobulin-Molekülen in eukaryontischen Zellen
ATE363490T1 (de) Methode zur produktion monoklonaler antikörper
ATE334954T1 (de) Verwendung von molekularsieben zur umsetzung von sauerstoffverbindungen in leichte olefine
ATE272669T1 (de) Neue thioether-derivate, verfahren zu ihrer herstellung und ihre verwendung
DE50110561D1 (de) Non-metallocene, verfahren zur herstellung von diesen und deren verwendung zur polymerisation von olefinen

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1340088

Country of ref document: EP