JP2010207220A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2010207220A5 JP2010207220A5 JP2010052206A JP2010052206A JP2010207220A5 JP 2010207220 A5 JP2010207220 A5 JP 2010207220A5 JP 2010052206 A JP2010052206 A JP 2010052206A JP 2010052206 A JP2010052206 A JP 2010052206A JP 2010207220 A5 JP2010207220 A5 JP 2010207220A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- labeled
- oligonucleotides
- moiety
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004707 nucleic acids Proteins 0.000 claims 23
- 150000007523 nucleic acids Chemical class 0.000 claims 23
- 102000039446 nucleic acids Human genes 0.000 claims 23
- 108091034117 Oligonucleotide Proteins 0.000 claims 18
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 13
- 238000000034 method Methods 0.000 claims 8
- 238000002844 melting Methods 0.000 claims 5
- 230000008018 melting Effects 0.000 claims 5
- 239000011541 reaction mixture Substances 0.000 claims 5
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- 230000000295 complement effect Effects 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 2
- 239000007850 fluorescent dye Substances 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 238000011109 contamination Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/400,966 | 2009-03-10 | ||
| US12/400,966 US8039215B2 (en) | 2009-03-10 | 2009-03-10 | Multiplex quantitative nucleic acid amplification and melting assay |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010207220A JP2010207220A (ja) | 2010-09-24 |
| JP2010207220A5 true JP2010207220A5 (cg-RX-API-DMAC7.html) | 2013-04-25 |
| JP5745774B2 JP5745774B2 (ja) | 2015-07-08 |
Family
ID=42224157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010052206A Active JP5745774B2 (ja) | 2009-03-10 | 2010-03-09 | 多重定量的核酸増幅及び融解アッセイ |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8039215B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2228454B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP5745774B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN101831496B (cg-RX-API-DMAC7.html) |
| CA (1) | CA2696652C (cg-RX-API-DMAC7.html) |
| ES (1) | ES2589102T3 (cg-RX-API-DMAC7.html) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2513747C (en) | 2003-01-24 | 2017-03-07 | University Of Utah | Methods of predicting mortality risk by determining telomere length |
| US8951939B2 (en) * | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| AU2009329987B2 (en) * | 2008-12-22 | 2016-08-04 | University Of Utah Research Foundation | Monochrome multiplex quantitative PCR |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| CN102576389B (zh) * | 2009-10-21 | 2016-08-03 | 皇家飞利浦电子股份有限公司 | 用于扩增反应的分析工具 |
| WO2012129187A1 (en) * | 2011-03-18 | 2012-09-27 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
| EP2530166B1 (en) * | 2011-05-31 | 2014-07-16 | ARKRAY, Inc. | Method for detecting a plurality of nucleotide polymorphisms at a single wavelength using a plurality of oligonucleotides modified with fluorescent dye having the same or close detection wavelength |
| CN104024433B (zh) * | 2011-10-31 | 2016-11-16 | 爱科来株式会社 | 基因丰度的测定方法 |
| WO2013074962A1 (en) * | 2011-11-16 | 2013-05-23 | Canon U.S. Life Sciences, Inc. | Mutation detection in higly homologous genomic regions |
| WO2013113748A1 (en) * | 2012-02-02 | 2013-08-08 | Primer Design Ltd | Method for detecting and genotyping target nucleic acid |
| CN107326085B (zh) | 2012-02-03 | 2022-05-03 | 加州理工学院 | 多路生化测定中信号的编码和解码 |
| EP2880184B1 (en) | 2012-08-03 | 2021-03-31 | California Institute of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| CN102952794B (zh) * | 2012-09-05 | 2015-02-25 | 张家港蓝苏生物工程有限公司 | 靶向基因dna分子探针的制备方法 |
| EP3967773A1 (en) * | 2012-09-10 | 2022-03-16 | BioFire Diagnostics, LLC | Multiple amplification cycle detection |
| WO2014142575A1 (en) | 2013-03-13 | 2014-09-18 | Seegene, Inc. | Quantification of target nucleic acid using melting peak analysis |
| JP6546158B2 (ja) | 2013-05-22 | 2019-07-17 | テロメア ダイアグノスティクス インコーポレイテッド | 短いテロメアの存在量の測定 |
| WO2015147370A1 (en) * | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| EP3034617A1 (en) | 2014-12-16 | 2016-06-22 | Marcos Isamat Riviere | Method for the simultaneous identification of multiple same-genome fragments or multiple fragments from different genomes using a PCR-technique |
| CN107208145A (zh) | 2014-12-30 | 2017-09-26 | 端粒诊断公司 | 多重定量pcr |
| KR102077577B1 (ko) * | 2015-06-04 | 2020-02-17 | 가부시키가이샤 미즈호 메디 | 복수의 표적 핵산의 검출 키트 및 그것을 사용하는 검출 방법 |
| CN116083543A (zh) | 2016-04-01 | 2023-05-09 | 克罗玛科德公司 | 用于工程化信号产生的竞争性探针 |
| CN109642252A (zh) | 2016-06-17 | 2019-04-16 | 加州理工学院 | 核酸反应及相关方法和组合物 |
| WO2018027185A1 (en) | 2016-08-04 | 2018-02-08 | Duke University | Compositions and methods for measuring bacterial growth |
| JP7221199B2 (ja) | 2016-09-15 | 2023-02-20 | エフ． ホフマン－ラ ロシュ エージー． | マルチプレックスｐｃｒの実施方法 |
| CN106868140B (zh) * | 2017-03-02 | 2022-10-18 | 北京酷搏科技有限公司 | 多重荧光定量pcr的方法 |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| EP3697927B1 (en) * | 2017-10-19 | 2022-12-14 | Bio-Rad Laboratories, Inc. | Digital amplification assays with unconventional and/or inverse changes in photoluminescence |
| AU2019209444A1 (en) * | 2018-01-22 | 2020-07-30 | Luminex Corporation | Methods and compositions for discrete melt analysis |
| CN112424377A (zh) | 2018-04-17 | 2021-02-26 | 克罗玛科德公司 | 用于多重分析的方法和系统 |
| EP3781708B1 (en) | 2018-04-20 | 2025-01-15 | Seegene, Inc. | Method for detecting a plurality of target nucleic acid sequences in sample |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| US20230068047A1 (en) * | 2021-08-19 | 2023-03-02 | Luminex Corporation | Digital amplification assay analysis method |
| WO2023125552A1 (zh) * | 2021-12-27 | 2023-07-06 | 迈克生物股份有限公司 | 对靶标核酸进行检测的方法 |
| EP4547861A1 (en) * | 2022-06-29 | 2025-05-07 | Life Technologies Corporation | Compositions, kits, and methods for detecting nucleic acids using intra-channel multiplexing |
| CN117701689A (zh) * | 2022-09-14 | 2024-03-15 | 迈克生物股份有限公司 | 多重pcr反应体系 |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| DE3529478A1 (de) | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung |
| US5795762A (en) | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5405774A (en) | 1986-08-22 | 1995-04-11 | Hoffmann-La Roche Inc. | DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 |
| US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| DK0550696T3 (da) | 1990-09-28 | 2001-03-19 | Hoffmann La Roche | Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus |
| JPH04234422A (ja) | 1990-10-31 | 1992-08-24 | Internatl Business Mach Corp <Ibm> | 二重硬化エポキシバックシール処方物 |
| DE4234086A1 (de) | 1992-02-05 | 1993-08-12 | Diagen Inst Molekularbio | Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| GB9513271D0 (en) | 1995-06-29 | 1995-09-06 | Johnson James G | Stress free fish harvester |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| ATE318327T1 (de) | 1996-06-04 | 2006-03-15 | Univ Utah Res Found | Fluoreszenz-donor-akzeptor paar |
| US6427156B1 (en) | 1997-01-21 | 2002-07-30 | Xilinx, Inc. | Configurable logic block with AND gate for efficient multiplication in FPGAS |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US6140054A (en) * | 1998-09-30 | 2000-10-31 | University Of Utah Research Foundation | Multiplex genotyping using fluorescent hybridization probes |
| US6472156B1 (en) | 1999-08-30 | 2002-10-29 | The University Of Utah | Homogeneous multiplex hybridization analysis by color and Tm |
| US20040053230A1 (en) | 2001-03-07 | 2004-03-18 | Schaffer Michael Edward | Real time quantitative pcr with intercalating dye for single and multiplex target dna |
| US7019129B1 (en) * | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
| CA2400601A1 (en) | 2001-08-31 | 2003-02-28 | University Of Utah Research Foundation | Real-time gene quantification with internal standards |
| US20040229253A1 (en) * | 2003-02-18 | 2004-11-18 | Hyldig-Nielsen Jens J. | Compositions and methods for multiplex analysis of polynucleotides |
| US20050053950A1 (en) * | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
| AU2005293703B2 (en) * | 2004-10-11 | 2010-02-18 | Epigenomics Ag | A method for the carry-over protection in DNA amplification systems targeting methylation analysis achieved by a modified pre-treatment of nucleic acids |
| JP5543065B2 (ja) | 2004-10-18 | 2014-07-09 | ブランデイズ ユニバーシティー | 核酸増幅のためのプライマー、プローブおよび方法 |
| US8067208B2 (en) | 2005-06-30 | 2011-11-29 | Roche Molecular Systems, Inc. | Probes and methods for hepatitis C virus typing using multidimensional probe analysis |
| JP4815980B2 (ja) * | 2005-10-05 | 2011-11-16 | 株式会社島津製作所 | 融解曲線測定による遺伝子増幅産物の解析方法 |
| WO2009042728A1 (en) | 2007-09-24 | 2009-04-02 | Allelogic Biosciences Corporation | Detection and /or quantification of nucleic acids |
| EP2116614A1 (en) | 2008-05-06 | 2009-11-11 | Qiagen GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
-
2009
- 2009-03-10 US US12/400,966 patent/US8039215B2/en active Active
-
2010
- 2010-03-08 ES ES10002350.6T patent/ES2589102T3/es active Active
- 2010-03-08 EP EP10002350.6A patent/EP2228454B1/en active Active
- 2010-03-09 CA CA2696652A patent/CA2696652C/en active Active
- 2010-03-09 JP JP2010052206A patent/JP5745774B2/ja active Active
- 2010-03-10 CN CN201010163977.7A patent/CN101831496B/zh active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2010207220A5 (cg-RX-API-DMAC7.html) | ||
| Kim et al. | Loop-mediated isothermal amplification-based nucleic acid lateral flow assay for the specific and multiplex detection of genetic markers | |
| CN104271767B (zh) | 多路生化测定中信号的编码和解码 | |
| TWI655419B (zh) | 分析試驗裝置、套組及使用方法 | |
| JP7477183B2 (ja) | 新規な等温単一反応用プローブセット及びその用途 | |
| CA2696652A1 (en) | Multiplex quantitative nucleic acid amplification and melting assay | |
| Yue et al. | Programmable strand displacement-based magnetic separation for simultaneous amplified detection of multiplex microRNAs by chemiluminescence imaging array | |
| JP2019522463A5 (cg-RX-API-DMAC7.html) | ||
| JP2014513557A5 (cg-RX-API-DMAC7.html) | ||
| Sapountzi et al. | Lateral flow devices for nucleic acid analysis exploiting quantum dots as reporters | |
| Bi et al. | Label-free chemiluminescent aptasensor for platelet-derived growth factor detection based on exonuclease-assisted cascade autocatalytic recycling amplification | |
| JP6431076B2 (ja) | リアルタイムｐｃｒ信号におけるジャンプの検出及び補正 | |
| WO2007115242A3 (en) | Binary probes for fluorescent analysis of nucleic acids | |
| Zhu et al. | Label‐Free Detection of MicroRNA: Two‐Step Signal Enhancement with a Hairpin‐Probe‐Based Graphene Fluorescence Switch and Isothermal Amplification | |
| Dong et al. | A signal-flexible gene diagnostic strategy coupling loop-mediated isothermal amplification with hybridization chain reaction | |
| JP2011530296A5 (cg-RX-API-DMAC7.html) | ||
| CN116083543A (zh) | 用于工程化信号产生的竞争性探针 | |
| CN104419765A (zh) | 核酸序列测定方法及装置、核酸序列测定用元件及其制造方法 | |
| WO2007087336A3 (en) | Methods for detecting nucleic acids using multiple signals | |
| Miao et al. | Terminal deoxynucleotidyl transferase-induced DNAzyme nanowire sensor for colorimetric detection of lipopolysaccharides | |
| JP2019536441A5 (cg-RX-API-DMAC7.html) | ||
| JP2021003050A5 (cg-RX-API-DMAC7.html) | ||
| Choi et al. | Point-of-care COVID-19 testing: colorimetric diagnosis using rapid and ultra-sensitive ramified rolling circle amplification | |
| Wang et al. | Colorimetric biosensor based on a DNAzyme primer and its application in logic gate operations for DNA screening | |
| JP2014533502A5 (cg-RX-API-DMAC7.html) |