CA2696652C - Multiplex quantitative nucleic acid amplification and melting assay - Google Patents

Multiplex quantitative nucleic acid amplification and melting assay Download PDF

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Publication number
CA2696652C
CA2696652C CA2696652A CA2696652A CA2696652C CA 2696652 C CA2696652 C CA 2696652C CA 2696652 A CA2696652 A CA 2696652A CA 2696652 A CA2696652 A CA 2696652A CA 2696652 C CA2696652 C CA 2696652C
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Prior art keywords
nucleic acid
target nucleic
melting
labeled
amplification
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CA2696652A
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English (en)
French (fr)
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CA2696652A1 (en
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Russell Higuchi
Cherie Holcomb
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2696652A 2009-03-10 2010-03-09 Multiplex quantitative nucleic acid amplification and melting assay Active CA2696652C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/400,966 2009-03-10
US12/400,966 US8039215B2 (en) 2009-03-10 2009-03-10 Multiplex quantitative nucleic acid amplification and melting assay

Publications (2)

Publication Number Publication Date
CA2696652A1 CA2696652A1 (en) 2010-09-10
CA2696652C true CA2696652C (en) 2015-09-08

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Family Applications (1)

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CA2696652A Active CA2696652C (en) 2009-03-10 2010-03-09 Multiplex quantitative nucleic acid amplification and melting assay

Country Status (6)

Country Link
US (1) US8039215B2 (cg-RX-API-DMAC7.html)
EP (1) EP2228454B1 (cg-RX-API-DMAC7.html)
JP (1) JP5745774B2 (cg-RX-API-DMAC7.html)
CN (1) CN101831496B (cg-RX-API-DMAC7.html)
CA (1) CA2696652C (cg-RX-API-DMAC7.html)
ES (1) ES2589102T3 (cg-RX-API-DMAC7.html)

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CN107208145A (zh) 2014-12-30 2017-09-26 端粒诊断公司 多重定量pcr
KR102077577B1 (ko) * 2015-06-04 2020-02-17 가부시키가이샤 미즈호 메디 복수의 표적 핵산의 검출 키트 및 그것을 사용하는 검출 방법
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JP7221199B2 (ja) 2016-09-15 2023-02-20 エフ． ホフマン－ラ ロシュ エージー． マルチプレックスｐｃｒの実施方法
CN106868140B (zh) * 2017-03-02 2022-10-18 北京酷搏科技有限公司 多重荧光定量pcr的方法
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
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AU2019209444A1 (en) * 2018-01-22 2020-07-30 Luminex Corporation Methods and compositions for discrete melt analysis
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
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US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
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WO2023125552A1 (zh) * 2021-12-27 2023-07-06 迈克生物股份有限公司 对靶标核酸进行检测的方法
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Also Published As

Publication number Publication date
ES2589102T3 (es) 2016-11-10
CN101831496A (zh) 2010-09-15
US8039215B2 (en) 2011-10-18
EP2228454B1 (en) 2016-06-22
US20100233686A1 (en) 2010-09-16
CN101831496B (zh) 2017-04-12
CA2696652A1 (en) 2010-09-10
JP2010207220A (ja) 2010-09-24
EP2228454A1 (en) 2010-09-15
JP5745774B2 (ja) 2015-07-08

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