JP2009542247A5 - - Google Patents

Info

Publication number

JP2009542247A5

JP2009542247A5 JP2009518958A JP2009518958A JP2009542247A5 JP 2009542247 A5 JP2009542247 A5 JP 2009542247A5 JP 2009518958 A JP2009518958 A JP 2009518958A JP 2009518958 A JP2009518958 A JP 2009518958A JP 2009542247 A5 JP2009542247 A5 JP 2009542247A5

Authority

JP

Japan

Prior art keywords

cell culture

embryonic stem

culture medium

ascorbic acid

cell

Prior art date

2007-07-10

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• DGVVWUTYPXICAM-UHFFFAOYSA-N 2-mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 14
• ZQPPMHVWECSIRJ-KTKRTIGZSA-N Oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims 14
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 14
• 241000288906 Primates Species 0.000 claims 12
• 239000006143 cell culture media Substances 0.000 claims 12
• 210000001671 Embryonic Stem Cells Anatomy 0.000 claims 11
• 102000018233 Fibroblast growth factor family Human genes 0.000 claims 8
• 108050007372 Fibroblast growth factor family Proteins 0.000 claims 8
• ZFGMDIBRIDKWMY-PASTXAENSA-N Heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 claims 8
• 229960002897 Heparin Drugs 0.000 claims 8
• 229920000669 heparin Polymers 0.000 claims 8
• 108091003117 Bovine Serum Albumin Proteins 0.000 claims 7
• 102000004877 Insulin Human genes 0.000 claims 7
• 108090001061 Insulin Proteins 0.000 claims 7
• 239000005642 Oleic acid Substances 0.000 claims 7
• MHQOTKLEMKRJIR-UHFFFAOYSA-L Sodium selenate Chemical compound [Na+].[Na+].[O-][Se]([O-])(=O)=O MHQOTKLEMKRJIR-UHFFFAOYSA-L 0.000 claims 7
• 102000004338 Transferrin Human genes 0.000 claims 7
• 108090000901 Transferrin Proteins 0.000 claims 7
• HZAXFHJVJLSVMW-UHFFFAOYSA-N ethanolamine Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims 7
• 229960001881 sodium selenate Drugs 0.000 claims 7
• 235000018716 sodium selenate Nutrition 0.000 claims 7
• 239000011655 sodium selenate Substances 0.000 claims 7
• 239000012581 transferrin Substances 0.000 claims 7
• 150000000996 L-ascorbic acids Chemical class 0.000 claims 6
• CIWBSHSKHKDKBQ-JLAZNSOCSA-N Vitamin C Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 6
• 229960005070 ascorbic acid Drugs 0.000 claims 6
• 235000010323 ascorbic acid Nutrition 0.000 claims 6
• 239000011668 ascorbic acid Substances 0.000 claims 6
• 238000004113 cell culture Methods 0.000 claims 6
• 210000004027 cells Anatomy 0.000 claims 5
• 102000004169 proteins and genes Human genes 0.000 claims 4
• 108090000623 proteins and genes Proteins 0.000 claims 4
• 235000014113 dietary fatty acids Nutrition 0.000 claims 3
• 239000000194 fatty acid Substances 0.000 claims 3
• 150000004665 fatty acids Chemical class 0.000 claims 3
• 210000002966 Serum Anatomy 0.000 claims 2
• 230000004069 differentiation Effects 0.000 claims 2
• 210000001161 Embryo, Mammalian Anatomy 0.000 claims 1
• 239000002609 media Substances 0.000 claims 1
• 238000000034 method Methods 0.000 claims 1
• 241000894007 species Species 0.000 claims 1
• 210000000130 stem cell Anatomy 0.000 claims 1