JP2009542247A5 - - Google Patents
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- JP2009542247A5 JP2009542247A5 JP2009518958A JP2009518958A JP2009542247A5 JP 2009542247 A5 JP2009542247 A5 JP 2009542247A5 JP 2009518958 A JP2009518958 A JP 2009518958A JP 2009518958 A JP2009518958 A JP 2009518958A JP 2009542247 A5 JP2009542247 A5 JP 2009542247A5
- Authority
- JP
- Japan
- Prior art keywords
- cell culture
- embryonic stem
- culture medium
- ascorbic acid
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- DGVVWUTYPXICAM-UHFFFAOYSA-N 2-mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 14
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N Oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims 14
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 14
- 241000288906 Primates Species 0.000 claims 12
- 239000006143 cell culture media Substances 0.000 claims 12
- 210000001671 Embryonic Stem Cells Anatomy 0.000 claims 11
- 102000018233 Fibroblast growth factor family Human genes 0.000 claims 8
- 108050007372 Fibroblast growth factor family Proteins 0.000 claims 8
- ZFGMDIBRIDKWMY-PASTXAENSA-N Heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 claims 8
- 229960002897 Heparin Drugs 0.000 claims 8
- 229920000669 heparin Polymers 0.000 claims 8
- 108091003117 Bovine Serum Albumin Proteins 0.000 claims 7
- 102000004877 Insulin Human genes 0.000 claims 7
- 108090001061 Insulin Proteins 0.000 claims 7
- 239000005642 Oleic acid Substances 0.000 claims 7
- MHQOTKLEMKRJIR-UHFFFAOYSA-L Sodium selenate Chemical compound [Na+].[Na+].[O-][Se]([O-])(=O)=O MHQOTKLEMKRJIR-UHFFFAOYSA-L 0.000 claims 7
- 102000004338 Transferrin Human genes 0.000 claims 7
- 108090000901 Transferrin Proteins 0.000 claims 7
- HZAXFHJVJLSVMW-UHFFFAOYSA-N ethanolamine Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims 7
- 229960001881 sodium selenate Drugs 0.000 claims 7
- 235000018716 sodium selenate Nutrition 0.000 claims 7
- 239000011655 sodium selenate Substances 0.000 claims 7
- 239000012581 transferrin Substances 0.000 claims 7
- 150000000996 L-ascorbic acids Chemical class 0.000 claims 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Vitamin C Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 6
- 229960005070 ascorbic acid Drugs 0.000 claims 6
- 235000010323 ascorbic acid Nutrition 0.000 claims 6
- 239000011668 ascorbic acid Substances 0.000 claims 6
- 238000004113 cell culture Methods 0.000 claims 6
- 210000004027 cells Anatomy 0.000 claims 5
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 235000014113 dietary fatty acids Nutrition 0.000 claims 3
- 239000000194 fatty acid Substances 0.000 claims 3
- 150000004665 fatty acids Chemical class 0.000 claims 3
- 210000002966 Serum Anatomy 0.000 claims 2
- 230000004069 differentiation Effects 0.000 claims 2
- 210000001161 Embryo, Mammalian Anatomy 0.000 claims 1
- 239000002609 media Substances 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
Claims (8)
ｉ）タンパク質より成る基礎的な培養支持体にてコートした培養容器を用い、該培養容器の中でインシュリン、トランスフェリン、セレン酸ナトリウム、エタノールアミン、２−メルカプトエタノール、脂肪酸除去したウシアルブミンと複合させたオレイン酸より構成され、更に線維芽細胞増殖因子、ヘパリンやアスコルビン酸又はリン酸化アスコルビン酸、又はこれらの誘導体を添加した細胞培養培地により霊長類胚性幹細胞を調製する工程、及び
ｉｉ）該霊長類胚性幹細胞を未分化状態で維持する工程、
より構成される、フィーダー細胞や血清のない細胞培養条件で、霊長類の胚性幹細胞を維持するための方法。 The following steps i) and ii):
i) Using a culture vessel coated with a basic culture support made of protein, in which it is combined with insulin, transferrin, sodium selenate, ethanolamine, 2-mercaptoethanol, fatty acid-free bovine albumin. A primate embryonic stem cell prepared from a cell culture medium comprising oleic acid and further supplemented with fibroblast growth factor, heparin, ascorbic acid, phosphorylated ascorbic acid, or a derivative thereof; and ii) the primate Maintaining embryonic stem cells in an undifferentiated state;
A method for maintaining primate embryonic stem cells in cell culture conditions free of feeder cells or serum.
ｉ）タンパク質より成る基礎的な培養支持体にてコートした培養容器を用い、インシュリン、トランスフェリン、セレン酸ナトリウム、エタノールアミン、２−メルカプトエタノール、脂肪酸除去したウシアルブミンと複合させたオレイン酸より構成され、更に線維芽細胞増殖因子及びヘパリンを添加した細胞培養培地により霊長類胚性幹細胞を調製する工程、及び
ｉｉ）少なくとも１細胞種への該霊長類胚性幹細胞の分化を誘導する因子を添加する工程、
より構成される、フィーダー細胞や血清のない細胞培養条件で、霊長類の胚性幹細胞を少なくとも１細胞種に分化させるための方法。 The following steps i) and ii):
i) It consists of oleic acid complexed with insulin, transferrin, sodium selenate, ethanolamine, 2-mercaptoethanol, bovine albumin from which fatty acids have been removed, using a culture vessel coated with a basic culture support consisting of protein. And ii) adding a factor that induces differentiation of the primate embryonic stem cell into at least one cell type, further comprising the step of preparing a primate embryonic stem cell with a cell culture medium supplemented with fibroblast growth factor and heparin. Process,
A method for differentiating primate embryonic stem cells into at least one cell type under feeder cell or serum-free cell culture conditions.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0613756.6A GB0613756D0 (en) | 2006-07-12 | 2006-07-12 | Cell culture medium |
| GB0613756.6 | 2006-07-12 | ||
| PCT/GB2007/002584 WO2008007082A2 (en) | 2006-07-12 | 2007-07-10 | Cell growth medium |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009542247A JP2009542247A (en) | 2009-12-03 |
| JP2009542247A5 true JP2009542247A5 (en) | 2010-10-07 |
| JP5227318B2 JP5227318B2 (en) | 2013-07-03 |
Family
ID=36955441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009518958A Active JP5227318B2 (en) | 2006-07-12 | 2007-07-10 | Cell growth medium |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP2038404A2 (en) |
| JP (1) | JP5227318B2 (en) |
| CN (1) | CN101490243A (en) |
| AU (1) | AU2007274065A1 (en) |
| CA (1) | CA2657539A1 (en) |
| GB (2) | GB0613756D0 (en) |
| WO (1) | WO2008007082A2 (en) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1962719A4 (en) | 2005-08-29 | 2011-05-04 | Technion Res And Dev Of Foundation Ltd | Media for culturing stem cells |
| DK3441459T3 (en) | 2006-08-02 | 2021-06-07 | Technion Res & Dev Foundation | PROCEDURES FOR EXPANSION OF EMBRYONAL STEM CELLS IN A SUSPENSION CULTURE |
| US9688956B2 (en) | 2008-06-05 | 2017-06-27 | National Cheng Kung University | Method for preserving proliferation and differentiation potential of mesenchymal stem cells |
| CN102076280B (en) | 2008-06-24 | 2014-08-27 | 生物活性外科公司 | Surgical sutures incorporated with stem cells or other bioactive materials |
| KR20110042107A (en) | 2008-08-07 | 2011-04-22 | 바이오엑티브 써지컬, 아이엔씨. | Stem cell capture and immobilization coatings for medical devices and implants |
| CN102317442B (en) * | 2008-12-17 | 2014-08-13 | 斯克里普斯研究所 | Generation and maintenance of stem cells |
| TW201109440A (en) * | 2009-07-21 | 2011-03-16 | Transgene Sa | Enzymatic composition for the digestion of chicken embryos |
| JP6276918B2 (en) | 2009-11-12 | 2018-02-07 | テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド | Medium, cell culture and method for culturing pluripotent stem cells in undifferentiated state |
| CN101812480B (en) * | 2009-11-16 | 2013-04-10 | 西北农林科技大学 | Method utilizing transcription factor to transfect bovine body cell into induced pluripotent stem cell |
| JP5714267B2 (en) * | 2010-08-26 | 2015-05-07 | 日本メナード化粧品株式会社 | Stem cell undifferentiation maintenance agent and proliferation promoter |
| JP6148429B2 (en) * | 2011-01-31 | 2017-06-14 | 協和発酵バイオ株式会社 | Method for culturing human pluripotent stem cells |
| CN102586176B (en) * | 2012-01-11 | 2013-05-08 | 中国科学院生物物理研究所 | Novel animal source-free and feed layer-free human pluripotent stem cell culture system |
| GB201211873D0 (en) * | 2012-07-04 | 2012-08-15 | Univ Edinburgh | Cell culture |
| SG11201505965TA (en) * | 2013-01-31 | 2015-09-29 | Ajinomoto Kk | Culture method for stable undifferentiated proliferation of pluripotent stem cells |
| SG11201509767UA (en) * | 2013-05-30 | 2015-12-30 | Ajinomoto Kk | Medium for culturing stem cells |
| CN105358707B (en) * | 2013-06-28 | 2019-06-14 | 株式会社钟化 | The method for screening multipotential stem cell positive growth factor |
| WO2015042356A1 (en) * | 2013-09-19 | 2015-03-26 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Chemically defined culture medium for stem cell maintenance and differentiation |
| CN104357379B (en) * | 2014-09-30 | 2017-08-08 | 刘兴宇 | Stem cell media |
| CN106032527B (en) * | 2015-03-17 | 2019-08-13 | 广州市搏克肿瘤研究所 | It is a kind of tolerance low-density without feeder layer human pluripotent stem cells culture medium |
| CN105907705B (en) * | 2016-06-28 | 2020-01-24 | 广州市搏克生物技术有限公司 | Pluripotent stem cell culture medium |
| JP7049347B2 (en) * | 2017-01-23 | 2022-04-06 | ステムセル テクノロジーズ カナダ インコーポレイテッド | Mediums and Methods to Enhance Stem Cell Survival and Proliferation |
| CN106754715A (en) * | 2017-02-13 | 2017-05-31 | 四川新生命干细胞科技股份有限公司 | A kind of trophoblastic preparation method for candidate stem cell culture |
| CN106754652B (en) * | 2017-03-06 | 2019-04-02 | 广州润虹医药科技股份有限公司 | IPS cell differentiation at ectoderm progenitor cells serum-free induced medium and abductive approach |
| CN106754657B (en) * | 2017-03-28 | 2022-07-22 | 北京赛斯达生物技术有限公司 | Serum-free medium for monkey embryonic stem cells |
| ES2955861T3 (en) * | 2017-05-31 | 2023-12-07 | Promocell Gmbh | Culture medium for pluripotent stem cells |
| CN110157662A (en) * | 2018-02-15 | 2019-08-23 | 郭永珍 | A kind of cell additive and preparation method thereof |
| KR102147780B1 (en) * | 2018-03-16 | 2020-08-25 | 사회복지법인 삼성생명공익재단 | Culture medium composition for promoting the proliferation of stem cell comprising FGF-17 as an effective ingredient and method of culturing stem cells using the same |
| CA3104838A1 (en) * | 2018-06-27 | 2020-01-02 | Ajinomoto Co., Inc. | Additive for culturing stem cells, culturing medium, and culturing method |
| CN110684716A (en) * | 2018-07-08 | 2020-01-14 | 郭永珍 | Preparation method of cell additive and product thereof |
| CN109486766B (en) * | 2018-11-26 | 2021-05-07 | 中山大学 | Lacrimal gland stem cell, culture system and culture method of lacrimal gland stem cell |
| CN111484970B (en) * | 2020-04-30 | 2022-09-16 | 广州再生医学与健康广东省实验室 | Serum-free and feeder-layer-free embryo and pluripotent stem cell culture medium with low protein content |
| WO2023150293A2 (en) * | 2022-02-03 | 2023-08-10 | Steakholder Foods Ltd. | Accelerated myotube formation |
| CN116210647A (en) * | 2023-02-03 | 2023-06-06 | 威奥福生物科技(宁波)有限公司 | Method for propagating white-feather broiler chickens by using primordial germ cell stem cell line |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5734998A (en) * | 1997-01-10 | 1998-08-03 | Life Technologies, Inc. | Embryonic stem cell serum replacement |
| US7439064B2 (en) * | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| WO2003029443A1 (en) * | 2001-09-28 | 2003-04-10 | Es Cell International Pte Ltd | Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers |
| CA2527847C (en) * | 2003-06-12 | 2015-09-29 | Yeda Research & Development Co. Ltd | Enhancement of oligodendrocyte differentiation |
| EP1698690B1 (en) * | 2003-12-26 | 2010-04-28 | Makoto Asashima | Basal medium for es cell culturing |
| GB2431165B (en) * | 2004-07-13 | 2009-04-01 | Geron Corp | Medium for growing human embryonic stem cells |
| GB2432846B (en) * | 2004-09-08 | 2009-12-30 | Wisconsin Alumni Res Found | Medium and culture of embryonic stem cells |
-
2006
- 2006-07-12 GB GBGB0613756.6A patent/GB0613756D0/en not_active Ceased
-
2007
- 2007-07-10 WO PCT/GB2007/002584 patent/WO2008007082A2/en active Application Filing
- 2007-07-10 CA CA002657539A patent/CA2657539A1/en not_active Abandoned
- 2007-07-10 JP JP2009518958A patent/JP5227318B2/en active Active
- 2007-07-10 EP EP07766180A patent/EP2038404A2/en not_active Withdrawn
- 2007-07-10 AU AU2007274065A patent/AU2007274065A1/en not_active Abandoned
- 2007-07-10 CN CNA2007800249934A patent/CN101490243A/en active Pending
-
2008
- 2008-12-29 GB GB0823575A patent/GB2452456A/en not_active Withdrawn
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