CA2657539A1 - Cell growth medium - Google Patents

Cell growth medium Download PDF

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Publication number
CA2657539A1
CA2657539A1 CA002657539A CA2657539A CA2657539A1 CA 2657539 A1 CA2657539 A1 CA 2657539A1 CA 002657539 A CA002657539 A CA 002657539A CA 2657539 A CA2657539 A CA 2657539A CA 2657539 A1 CA2657539 A1 CA 2657539A1
Authority
CA
Canada
Prior art keywords
cell culture
stem cells
embryonic stem
cell
primate embryonic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002657539A
Other languages
English (en)
French (fr)
Inventor
Miho Furue
Peter Andrews
Tetsuji Okamoto
Denry J Sato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Sheffield
Original Assignee
University of Sheffield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Sheffield filed Critical University of Sheffield
Publication of CA2657539A1 publication Critical patent/CA2657539A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/44Thiols, e.g. mercaptoethanol
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/46Amines, e.g. putrescine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90Polysaccharides
    • C12N2501/91Heparin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA002657539A 2006-07-12 2007-07-10 Cell growth medium Abandoned CA2657539A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0613756.6 2006-07-12
GBGB0613756.6A GB0613756D0 (en) 2006-07-12 2006-07-12 Cell culture medium
PCT/GB2007/002584 WO2008007082A2 (en) 2006-07-12 2007-07-10 Cell growth medium

Publications (1)

Publication Number Publication Date
CA2657539A1 true CA2657539A1 (en) 2008-01-17

Family

ID=36955441

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002657539A Abandoned CA2657539A1 (en) 2006-07-12 2007-07-10 Cell growth medium

Country Status (7)

Country Link
EP (1) EP2038404A2 (zh)
JP (1) JP5227318B2 (zh)
CN (1) CN101490243A (zh)
AU (1) AU2007274065A1 (zh)
CA (1) CA2657539A1 (zh)
GB (2) GB0613756D0 (zh)
WO (1) WO2008007082A2 (zh)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3354723B1 (en) 2005-08-29 2023-12-13 Technion Research & Development Foundation Ltd. Media for culturing stem cells
EP2733203B1 (en) 2006-08-02 2018-10-10 Technion Research & Development Foundation Ltd. Methods of expanding embryonic stem cells in a suspension culture
US9688956B2 (en) 2008-06-05 2017-06-27 National Cheng Kung University Method for preserving proliferation and differentiation potential of mesenchymal stem cells
CA2765670C (en) 2008-06-24 2018-05-15 Bioactive Surgical, Inc. Surgical sutures incorporated with stem cells or other bioactive materials
CN102176882A (zh) 2008-08-07 2011-09-07 生物活性外科公司 医疗器械和植入物的干细胞捕获和固定涂层
CN102317442B (zh) * 2008-12-17 2014-08-13 斯克里普斯研究所 干细胞的产生和保持
US20120190100A1 (en) * 2009-07-21 2012-07-26 Transgene AS Enzymatic composition for the digestion of chicken embryos
ES2779048T3 (es) * 2009-11-12 2020-08-13 Technion Res & Dev Foundation Medios de cultivo, cultivos celulares y métodos de cultivo de células madre pluripotentes en un estado indiferenciado
CN101812480B (zh) * 2009-11-16 2013-04-10 西北农林科技大学 利用转录因子转染牛体细胞成为诱导性多能干细胞的方法
JP5714267B2 (ja) * 2010-08-26 2015-05-07 日本メナード化粧品株式会社 幹細胞の未分化維持剤及び増殖促進剤
JP6148429B2 (ja) 2011-01-31 2017-06-14 協和発酵バイオ株式会社 ヒト多能性幹細胞の培養方法
CN102586176B (zh) * 2012-01-11 2013-05-08 中国科学院生物物理研究所 一种新型的无动物源、无饲养层的人多能干细胞培养系统
GB201211873D0 (en) * 2012-07-04 2012-08-15 Univ Edinburgh Cell culture
CN104955939B (zh) * 2013-01-31 2018-07-27 味之素株式会社 用于进行多能性干细胞的稳定的未分化维持增殖的培养方法
EP3006558B1 (en) * 2013-05-30 2020-05-13 Ajinomoto Co., Inc. Medium for culturing stem cells
US10066211B2 (en) * 2013-06-28 2018-09-04 Kaneka Corporation Method for screening for pluripotent stem cell growth-promoting factor
US20160230143A1 (en) * 2013-09-19 2016-08-11 The U.S.A., As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
CN104357379B (zh) * 2014-09-30 2017-08-08 刘兴宇 干细胞培养基
CN106032527B (zh) * 2015-03-17 2019-08-13 广州市搏克肿瘤研究所 一种耐受低密度的无饲养层人多能干细胞培养基
CN105907705B (zh) * 2016-06-28 2020-01-24 广州市搏克生物技术有限公司 一种多能干细胞培养基
EP4361256A3 (en) 2017-01-23 2024-07-31 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells
CN106754715A (zh) * 2017-02-13 2017-05-31 四川新生命干细胞科技股份有限公司 一种用于造血干细胞培养的滋养层的制备方法
CN106754652B (zh) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106754657B (zh) * 2017-03-28 2022-07-22 北京赛斯达生物技术有限公司 一种猴胚胎干细胞的无血清培养基
US20200332263A1 (en) * 2017-05-31 2020-10-22 Promocell Gmbh Culture medium for pluripotent stem cells
CN110157662A (zh) * 2018-02-15 2019-08-23 郭永珍 一种细胞添加剂及其制备方法
KR102147780B1 (ko) * 2018-03-16 2020-08-25 사회복지법인 삼성생명공익재단 Fgf-17을 유효 성분으로 포함하는 줄기세포 증식 촉진용 배지 조성물 및 이를 이용한 줄기세포 증식 촉진 방법
EP3816279A4 (en) * 2018-06-27 2022-04-20 Ajinomoto Co., Inc. ADDITIVE FOR CULTIVATION OF STEM CELLS, CULTURE MEDIUM AND CULTIVATION PROCEDURE
CN110684716A (zh) * 2018-07-08 2020-01-14 郭永珍 一种细胞添加剂的制备方法及其产品
CN109486766B (zh) * 2018-11-26 2021-05-07 中山大学 一种泪腺干细胞、泪腺干细胞的培养体系和培养方法
CN111484970B (zh) * 2020-04-30 2022-09-16 广州再生医学与健康广东省实验室 一种低蛋白含量的无血清无饲养层胚胎与多能干细胞培养基
WO2023150293A2 (en) * 2022-02-03 2023-08-10 Steakholder Foods Ltd. Accelerated myotube formation
CN116210647A (zh) * 2023-02-03 2023-06-06 威奥福生物科技(宁波)有限公司 一种使用原始生殖细胞干细胞系扩繁白羽肉鸡的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001508302A (ja) * 1997-01-10 2001-06-26 ライフ テクノロジーズ,インコーポレイテッド 胚性幹細胞血清置換
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
WO2003029443A1 (en) * 2001-09-28 2003-04-10 Es Cell International Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
WO2004111210A1 (en) * 2003-06-12 2004-12-23 Yeda Research & Development Co.Ltd Enhancement of oligodendrocyte differentiation
CA2552229C (en) * 2003-12-26 2013-03-12 Miho Furue Basal medium for es cell culturing
EP1809739B1 (en) * 2004-07-13 2014-10-15 Asterias Biotherapeutics, Inc. Medium for growing human embryonic stem cells
JP5420837B2 (ja) * 2004-09-08 2014-02-19 ウィスコンシン アラムニ リサーチ ファンデーション 胚幹細胞の培地及び培養

Also Published As

Publication number Publication date
JP5227318B2 (ja) 2013-07-03
WO2008007082A2 (en) 2008-01-17
WO2008007082A3 (en) 2008-03-06
GB2452456A (en) 2009-03-04
JP2009542247A (ja) 2009-12-03
AU2007274065A1 (en) 2008-01-17
GB0613756D0 (en) 2006-08-23
GB0823575D0 (en) 2009-01-28
EP2038404A2 (en) 2009-03-25
CN101490243A (zh) 2009-07-22

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Legal Events

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FZDE Discontinued