CA2657539A1 - Milieu de croissance cellulaire - Google Patents

Milieu de croissance cellulaire Download PDF

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Publication number
CA2657539A1
CA2657539A1 CA002657539A CA2657539A CA2657539A1 CA 2657539 A1 CA2657539 A1 CA 2657539A1 CA 002657539 A CA002657539 A CA 002657539A CA 2657539 A CA2657539 A CA 2657539A CA 2657539 A1 CA2657539 A1 CA 2657539A1
Authority
CA
Canada
Prior art keywords
cell culture
stem cells
embryonic stem
cell
primate embryonic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002657539A
Other languages
English (en)
Inventor
Miho Furue
Peter Andrews
Tetsuji Okamoto
Denry J Sato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Sheffield
Original Assignee
University of Sheffield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Sheffield filed Critical University of Sheffield
Publication of CA2657539A1 publication Critical patent/CA2657539A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA002657539A 2006-07-12 2007-07-10 Milieu de croissance cellulaire Abandoned CA2657539A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0613756.6A GB0613756D0 (en) 2006-07-12 2006-07-12 Cell culture medium
GB0613756.6 2006-07-12
PCT/GB2007/002584 WO2008007082A2 (fr) 2006-07-12 2007-07-10 Milieu de croissance cellulaire

Publications (1)

Publication Number Publication Date
CA2657539A1 true CA2657539A1 (fr) 2008-01-17

Family

ID=36955441

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002657539A Abandoned CA2657539A1 (fr) 2006-07-12 2007-07-10 Milieu de croissance cellulaire

Country Status (7)

Country Link
EP (1) EP2038404A2 (fr)
JP (1) JP5227318B2 (fr)
CN (1) CN101490243A (fr)
AU (1) AU2007274065A1 (fr)
CA (1) CA2657539A1 (fr)
GB (2) GB0613756D0 (fr)
WO (1) WO2008007082A2 (fr)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1962719A4 (fr) 2005-08-29 2011-05-04 Technion Res And Dev Of Foundation Ltd Milieux de culture de cellules souches
DK2733203T3 (en) 2006-08-02 2019-02-04 Technion Res & Dev Foundation PROCEDURES FOR EXPANSION OF EMBRYONAL STEM CELLS IN A SUSPENSION CULTURE
US9688956B2 (en) 2008-06-05 2017-06-27 National Cheng Kung University Method for preserving proliferation and differentiation potential of mesenchymal stem cells
CN104274221B (zh) 2008-06-24 2017-04-12 生物活性外科公司 掺入有干细胞或其它生物活性物质的外科缝合线
CA2769878A1 (fr) 2008-08-07 2010-02-11 Bioactive Surgical, Inc. Revetements de capture et d'immobilisation de cellules souches pour dispositifs et implants medicaux
US8906677B2 (en) * 2008-12-17 2014-12-09 The Scripps Research Institute Generation and maintenance of stem cells
SG178090A1 (en) * 2009-07-21 2012-03-29 Transgene Sa Enzymatic composition for the digestion of chicken embryos
WO2011058558A2 (fr) 2009-11-12 2011-05-19 Technion Research & Development Foundation Ltd. Milieu de culture, cultures de cellules et procédés de culture de cellules souches pluripotentes dans un état indifférencié
CN101812480B (zh) * 2009-11-16 2013-04-10 西北农林科技大学 利用转录因子转染牛体细胞成为诱导性多能干细胞的方法
JP5714267B2 (ja) * 2010-08-26 2015-05-07 日本メナード化粧品株式会社 幹細胞の未分化維持剤及び増殖促進剤
JP6148429B2 (ja) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 ヒト多能性幹細胞の培養方法
CN102586176B (zh) * 2012-01-11 2013-05-08 中国科学院生物物理研究所 一种新型的无动物源、无饲养层的人多能干细胞培养系统
GB201211873D0 (en) * 2012-07-04 2012-08-15 Univ Edinburgh Cell culture
SG11201505965TA (en) * 2013-01-31 2015-09-29 Ajinomoto Kk Culture method for stable undifferentiated proliferation of pluripotent stem cells
EP3006558B1 (fr) * 2013-05-30 2020-05-13 Ajinomoto Co., Inc. Milieu de culture de cellules souches
US10066211B2 (en) * 2013-06-28 2018-09-04 Kaneka Corporation Method for screening for pluripotent stem cell growth-promoting factor
US20160230143A1 (en) * 2013-09-19 2016-08-11 The U.S.A., As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
CN104357379B (zh) * 2014-09-30 2017-08-08 刘兴宇 干细胞培养基
CN106032527B (zh) * 2015-03-17 2019-08-13 广州市搏克肿瘤研究所 一种耐受低密度的无饲养层人多能干细胞培养基
CN105907705B (zh) * 2016-06-28 2020-01-24 广州市搏克生物技术有限公司 一种多能干细胞培养基
EP4361256A2 (fr) * 2017-01-23 2024-05-01 Stemcell Technologies Canada Inc. Milieux et procédés pour améliorer la survie et la prolifération de cellules souches
CN106754715A (zh) * 2017-02-13 2017-05-31 四川新生命干细胞科技股份有限公司 一种用于造血干细胞培养的滋养层的制备方法
CN106754652B (zh) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106754657B (zh) * 2017-03-28 2022-07-22 北京赛斯达生物技术有限公司 一种猴胚胎干细胞的无血清培养基
PL3436568T3 (pl) * 2017-05-31 2023-11-20 Promocell Gmbh Podłoże hodowlane do pluripotencjalnych komórek macierzystych
CN110157662A (zh) * 2018-02-15 2019-08-23 郭永珍 一种细胞添加剂及其制备方法
KR102147780B1 (ko) * 2018-03-16 2020-08-25 사회복지법인 삼성생명공익재단 Fgf-17을 유효 성분으로 포함하는 줄기세포 증식 촉진용 배지 조성물 및 이를 이용한 줄기세포 증식 촉진 방법
CA3104838A1 (fr) * 2018-06-27 2020-01-02 Ajinomoto Co., Inc. Additif pour culture de cellules souches, milieu de culture, et methode de culture
CN110684716A (zh) * 2018-07-08 2020-01-14 郭永珍 一种细胞添加剂的制备方法及其产品
CN109486766B (zh) * 2018-11-26 2021-05-07 中山大学 一种泪腺干细胞、泪腺干细胞的培养体系和培养方法
CN111484970B (zh) * 2020-04-30 2022-09-16 广州再生医学与健康广东省实验室 一种低蛋白含量的无血清无饲养层胚胎与多能干细胞培养基
WO2023150293A2 (fr) * 2022-02-03 2023-08-10 Steakholder Foods Ltd. Formation accélérée de myotube

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001508302A (ja) * 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
AU2002333022C1 (en) * 2001-09-28 2011-06-16 Es Cell International Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (HES) cells on feeder-free matrices and human feeder layers
WO2004111210A1 (fr) * 2003-06-12 2004-12-23 Yeda Research & Development Co.Ltd Amelioration de la differenciation d'oligodendrocytes
ATE466073T1 (de) * 2003-12-26 2010-05-15 Makoto Asashima Basalmedium zur kultivierung von es-zellen
GB2431165B (en) * 2004-07-13 2009-04-01 Geron Corp Medium for growing human embryonic stem cells
AU2005282510B2 (en) * 2004-09-08 2010-12-02 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells

Also Published As

Publication number Publication date
AU2007274065A1 (en) 2008-01-17
EP2038404A2 (fr) 2009-03-25
GB2452456A (en) 2009-03-04
CN101490243A (zh) 2009-07-22
GB0613756D0 (en) 2006-08-23
JP5227318B2 (ja) 2013-07-03
WO2008007082A3 (fr) 2008-03-06
WO2008007082A2 (fr) 2008-01-17
GB0823575D0 (en) 2009-01-28
JP2009542247A (ja) 2009-12-03

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Legal Events

Date Code Title Description
FZDE Discontinued