CN101490243A - 细胞生长培养基 - Google Patents

细胞生长培养基 Download PDF

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Publication number
CN101490243A
CN101490243A CNA2007800249934A CN200780024993A CN101490243A CN 101490243 A CN101490243 A CN 101490243A CN A2007800249934 A CNA2007800249934 A CN A2007800249934A CN 200780024993 A CN200780024993 A CN 200780024993A CN 101490243 A CN101490243 A CN 101490243A
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cell
cell culture
embryonic stem
stem cells
culture medium
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Chinese (zh)
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古江美保
彼得·安德鲁斯
冈本哲治
丹瑞·J·佐藤
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University of Sheffield
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University of Sheffield
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CNA2007800249934A 2006-07-12 2007-07-10 细胞生长培养基 Pending CN101490243A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0613756.6 2006-07-12
GBGB0613756.6A GB0613756D0 (en) 2006-07-12 2006-07-12 Cell culture medium

Publications (1)

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CN101490243A true CN101490243A (zh) 2009-07-22

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CNA2007800249934A Pending CN101490243A (zh) 2006-07-12 2007-07-10 细胞生长培养基

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EP (1) EP2038404A2 (fr)
JP (1) JP5227318B2 (fr)
CN (1) CN101490243A (fr)
AU (1) AU2007274065A1 (fr)
CA (1) CA2657539A1 (fr)
GB (2) GB0613756D0 (fr)
WO (1) WO2008007082A2 (fr)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101812480A (zh) * 2009-11-16 2010-08-25 西北农林科技大学 利用转录因子转染牛体细胞成为诱导性多能干细胞的方法
CN102586176A (zh) * 2012-01-11 2012-07-18 中国科学院生物物理研究所 一种新型的无动物源、无饲养层的人多能干细胞培养系统
CN102939100A (zh) * 2009-07-21 2013-02-20 特兰斯吉恩股份有限公司 用于消化鸡胚胎的酶组合物
CN104357379A (zh) * 2014-09-30 2015-02-18 刘兴宇 干细胞培养基
CN104955939A (zh) * 2013-01-31 2015-09-30 味之素株式会社 用于进行多能性干细胞的稳定的未分化维持增殖的培养方法
CN105907705A (zh) * 2016-06-28 2016-08-31 广州市搏克生物技术有限公司 一种多能干细胞培养基
CN106032527A (zh) * 2015-03-17 2016-10-19 广州市搏克肿瘤研究所 一种耐受低密度的无饲养层人多能干细胞培养基
CN106754652A (zh) * 2017-03-06 2017-05-31 广州润虹医药科技有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106754715A (zh) * 2017-02-13 2017-05-31 四川新生命干细胞科技股份有限公司 一种用于造血干细胞培养的滋养层的制备方法
CN106754657A (zh) * 2017-03-28 2017-05-31 北京赛斯达生物技术有限公司 一种猴胚胎干细胞的无血清培养基
CN109486766A (zh) * 2018-11-26 2019-03-19 中山大学 一种泪腺干细胞、泪腺干细胞的培养体系和培养方法
CN110157662A (zh) * 2018-02-15 2019-08-23 郭永珍 一种细胞添加剂及其制备方法
CN110684716A (zh) * 2018-07-08 2020-01-14 郭永珍 一种细胞添加剂的制备方法及其产品
CN111484970A (zh) * 2020-04-30 2020-08-04 广州再生医学与健康广东省实验室 一种低蛋白含量的无血清无饲养层胚胎与多能干细胞培养基
CN112313329A (zh) * 2018-06-27 2021-02-02 味之素株式会社 干细胞培养用添加物和培养用培养基、以及培养方法

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006286149B2 (en) 2005-08-29 2012-09-13 Technion Research And Development Foundation Ltd. Media for culturing stem cells
DK3441459T3 (da) 2006-08-02 2021-06-07 Technion Res & Dev Foundation Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur
US9688956B2 (en) 2008-06-05 2017-06-27 National Cheng Kung University Method for preserving proliferation and differentiation potential of mesenchymal stem cells
CN102076280B (zh) 2008-06-24 2014-08-27 生物活性外科公司 掺入有干细胞或其它生物活性物质的外科缝合线
WO2010017459A1 (fr) 2008-08-07 2010-02-11 Bioactive Surgical, Inc> Revêtements de capture et d’immobilisation de cellules souches pour dispositifs et implants médicaux
CN102317442B (zh) * 2008-12-17 2014-08-13 斯克里普斯研究所 干细胞的产生和保持
DK3633025T3 (da) * 2009-11-12 2022-12-12 Technion Res & Dev Foundation Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand
JP5714267B2 (ja) * 2010-08-26 2015-05-07 日本メナード化粧品株式会社 幹細胞の未分化維持剤及び増殖促進剤
JP6148429B2 (ja) 2011-01-31 2017-06-14 協和発酵バイオ株式会社 ヒト多能性幹細胞の培養方法
GB201211873D0 (en) * 2012-07-04 2012-08-15 Univ Edinburgh Cell culture
KR102229266B1 (ko) 2013-05-30 2021-03-19 아지노모토 가부시키가이샤 줄기 세포의 배양용 배지
CN105358707B (zh) * 2013-06-28 2019-06-14 株式会社钟化 筛选多能干细胞生长促进因子的方法
US20160230143A1 (en) * 2013-09-19 2016-08-11 The U.S.A., As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
WO2018132926A1 (fr) * 2017-01-23 2018-07-26 Stemcell Technologies Canada Inc. Milieux et procédés pour améliorer la survie et la prolifération de cellules souches
PL3436568T3 (pl) * 2017-05-31 2023-11-20 Promocell Gmbh Podłoże hodowlane do pluripotencjalnych komórek macierzystych
WO2019177420A1 (fr) * 2018-03-16 2019-09-19 사회복지법인 삼성생명공익재단 Composition de milieu comprenant du fgf -17 en tant qu'ingrédient efficace pour favoriser la prolifération de cellules souches et procédé de promotion de la prolifération de cellules souches à l'aide de celle-ci
WO2023150293A2 (fr) * 2022-02-03 2023-08-10 Steakholder Foods Ltd. Formation accélérée de myotube

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998030679A1 (fr) * 1997-01-10 1998-07-16 Life Technologies, Inc. Substitut de serum pour cellules souches embryonnaires
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
JP2005503822A (ja) * 2001-09-28 2005-02-10 イーエス・セル・インターナショナル・プライヴェート・リミテッド 無フィーダーマトリックス上での未分化ヒト胚性幹（ｈｅｓ）細胞の導出および増殖方法ならびにヒトフィーダー層
CA2527847C (fr) * 2003-06-12 2015-09-29 Yeda Research & Development Co. Ltd Amelioration de la differenciation d'oligodendrocytes
ATE466073T1 (de) * 2003-12-26 2010-05-15 Makoto Asashima Basalmedium zur kultivierung von es-zellen
AU2005271723B2 (en) * 2004-07-13 2010-12-16 Asterias Biotherapeutics, Inc. Medium for growing human embryonic stem cells
AU2005282510B2 (en) * 2004-09-08 2010-12-02 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102939100A (zh) * 2009-07-21 2013-02-20 特兰斯吉恩股份有限公司 用于消化鸡胚胎的酶组合物
CN101812480A (zh) * 2009-11-16 2010-08-25 西北农林科技大学 利用转录因子转染牛体细胞成为诱导性多能干细胞的方法
CN101812480B (zh) * 2009-11-16 2013-04-10 西北农林科技大学 利用转录因子转染牛体细胞成为诱导性多能干细胞的方法
CN102586176A (zh) * 2012-01-11 2012-07-18 中国科学院生物物理研究所 一种新型的无动物源、无饲养层的人多能干细胞培养系统
CN102586176B (zh) * 2012-01-11 2013-05-08 中国科学院生物物理研究所 一种新型的无动物源、无饲养层的人多能干细胞培养系统
CN104955939A (zh) * 2013-01-31 2015-09-30 味之素株式会社 用于进行多能性干细胞的稳定的未分化维持增殖的培养方法
CN104955939B (zh) * 2013-01-31 2018-07-27 味之素株式会社 用于进行多能性干细胞的稳定的未分化维持增殖的培养方法
CN104357379A (zh) * 2014-09-30 2015-02-18 刘兴宇 干细胞培养基
CN104357379B (zh) * 2014-09-30 2017-08-08 刘兴宇 干细胞培养基
CN106032527A (zh) * 2015-03-17 2016-10-19 广州市搏克肿瘤研究所 一种耐受低密度的无饲养层人多能干细胞培养基
CN106032527B (zh) * 2015-03-17 2019-08-13 广州市搏克肿瘤研究所 一种耐受低密度的无饲养层人多能干细胞培养基
CN105907705A (zh) * 2016-06-28 2016-08-31 广州市搏克生物技术有限公司 一种多能干细胞培养基
CN105907705B (zh) * 2016-06-28 2020-01-24 广州市搏克生物技术有限公司 一种多能干细胞培养基
CN106754715A (zh) * 2017-02-13 2017-05-31 四川新生命干细胞科技股份有限公司 一种用于造血干细胞培养的滋养层的制备方法
CN106754652B (zh) * 2017-03-06 2019-04-02 广州润虹医药科技股份有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106754652A (zh) * 2017-03-06 2017-05-31 广州润虹医药科技有限公司 iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法
CN106754657A (zh) * 2017-03-28 2017-05-31 北京赛斯达生物技术有限公司 一种猴胚胎干细胞的无血清培养基
CN110157662A (zh) * 2018-02-15 2019-08-23 郭永珍 一种细胞添加剂及其制备方法
CN112313329A (zh) * 2018-06-27 2021-02-02 味之素株式会社 干细胞培养用添加物和培养用培养基、以及培养方法
CN110684716A (zh) * 2018-07-08 2020-01-14 郭永珍 一种细胞添加剂的制备方法及其产品
CN109486766A (zh) * 2018-11-26 2019-03-19 中山大学 一种泪腺干细胞、泪腺干细胞的培养体系和培养方法
CN111484970A (zh) * 2020-04-30 2020-08-04 广州再生医学与健康广东省实验室 一种低蛋白含量的无血清无饲养层胚胎与多能干细胞培养基

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Publication number Publication date
AU2007274065A1 (en) 2008-01-17
WO2008007082A2 (fr) 2008-01-17
JP2009542247A (ja) 2009-12-03
CA2657539A1 (fr) 2008-01-17
JP5227318B2 (ja) 2013-07-03
WO2008007082A3 (fr) 2008-03-06
GB0823575D0 (en) 2009-01-28
EP2038404A2 (fr) 2009-03-25
GB2452456A (en) 2009-03-04
GB0613756D0 (en) 2006-08-23

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