JP5227318B2 - 細胞増殖培地 - Google Patents
細胞増殖培地 Download PDFInfo
- Publication number
- JP5227318B2 JP5227318B2 JP2009518958A JP2009518958A JP5227318B2 JP 5227318 B2 JP5227318 B2 JP 5227318B2 JP 2009518958 A JP2009518958 A JP 2009518958A JP 2009518958 A JP2009518958 A JP 2009518958A JP 5227318 B2 JP5227318 B2 JP 5227318B2
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- JP
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- Prior art keywords
- cell culture
- embryonic stem
- cells
- heparin
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/44—Thiols, e.g. mercaptoethanol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/46—Amines, e.g. putrescine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/90—Polysaccharides
- C12N2501/91—Heparin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Gynecology & Obstetrics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Reproductive Health (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0613756.6 | 2006-07-12 | ||
| GBGB0613756.6A GB0613756D0 (en) | 2006-07-12 | 2006-07-12 | Cell culture medium |
| PCT/GB2007/002584 WO2008007082A2 (en) | 2006-07-12 | 2007-07-10 | Cell growth medium |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009542247A JP2009542247A (ja) | 2009-12-03 |
| JP2009542247A5 JP2009542247A5 (zh) | 2010-10-07 |
| JP5227318B2 true JP5227318B2 (ja) | 2013-07-03 |
Family
ID=36955441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009518958A Active JP5227318B2 (ja) | 2006-07-12 | 2007-07-10 | 細胞増殖培地 |
Country Status (7)
| Country | Link |
|---|---|
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| JP (1) | JP5227318B2 (zh) |
| CN (1) | CN101490243A (zh) |
| AU (1) | AU2007274065A1 (zh) |
| CA (1) | CA2657539A1 (zh) |
| GB (2) | GB0613756D0 (zh) |
| WO (1) | WO2008007082A2 (zh) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP3354723B1 (en) | 2005-08-29 | 2023-12-13 | Technion Research & Development Foundation Ltd. | Media for culturing stem cells |
| EP2733203B1 (en) | 2006-08-02 | 2018-10-10 | Technion Research & Development Foundation Ltd. | Methods of expanding embryonic stem cells in a suspension culture |
| US9688956B2 (en) | 2008-06-05 | 2017-06-27 | National Cheng Kung University | Method for preserving proliferation and differentiation potential of mesenchymal stem cells |
| CA2765670C (en) | 2008-06-24 | 2018-05-15 | Bioactive Surgical, Inc. | Surgical sutures incorporated with stem cells or other bioactive materials |
| CN102176882A (zh) | 2008-08-07 | 2011-09-07 | 生物活性外科公司 | 医疗器械和植入物的干细胞捕获和固定涂层 |
| CN102317442B (zh) * | 2008-12-17 | 2014-08-13 | 斯克里普斯研究所 | 干细胞的产生和保持 |
| US20120190100A1 (en) * | 2009-07-21 | 2012-07-26 | Transgene AS | Enzymatic composition for the digestion of chicken embryos |
| ES2779048T3 (es) * | 2009-11-12 | 2020-08-13 | Technion Res & Dev Foundation | Medios de cultivo, cultivos celulares y métodos de cultivo de células madre pluripotentes en un estado indiferenciado |
| CN101812480B (zh) * | 2009-11-16 | 2013-04-10 | 西北农林科技大学 | 利用转录因子转染牛体细胞成为诱导性多能干细胞的方法 |
| JP5714267B2 (ja) * | 2010-08-26 | 2015-05-07 | 日本メナード化粧品株式会社 | 幹細胞の未分化維持剤及び増殖促進剤 |
| JP6148429B2 (ja) | 2011-01-31 | 2017-06-14 | 協和発酵バイオ株式会社 | ヒト多能性幹細胞の培養方法 |
| CN102586176B (zh) * | 2012-01-11 | 2013-05-08 | 中国科学院生物物理研究所 | 一种新型的无动物源、无饲养层的人多能干细胞培养系统 |
| GB201211873D0 (en) * | 2012-07-04 | 2012-08-15 | Univ Edinburgh | Cell culture |
| CN104955939B (zh) * | 2013-01-31 | 2018-07-27 | 味之素株式会社 | 用于进行多能性干细胞的稳定的未分化维持增殖的培养方法 |
| EP3006558B1 (en) * | 2013-05-30 | 2020-05-13 | Ajinomoto Co., Inc. | Medium for culturing stem cells |
| US10066211B2 (en) * | 2013-06-28 | 2018-09-04 | Kaneka Corporation | Method for screening for pluripotent stem cell growth-promoting factor |
| US20160230143A1 (en) * | 2013-09-19 | 2016-08-11 | The U.S.A., As Represented By The Secretary, Department Of Health & Human Services | Chemically defined culture medium for stem cell maintenance and differentiation |
| CN104357379B (zh) * | 2014-09-30 | 2017-08-08 | 刘兴宇 | 干细胞培养基 |
| CN106032527B (zh) * | 2015-03-17 | 2019-08-13 | 广州市搏克肿瘤研究所 | 一种耐受低密度的无饲养层人多能干细胞培养基 |
| CN105907705B (zh) * | 2016-06-28 | 2020-01-24 | 广州市搏克生物技术有限公司 | 一种多能干细胞培养基 |
| EP4361256A3 (en) | 2017-01-23 | 2024-07-31 | Stemcell Technologies Canada Inc. | Media and methods for enhancing the survival and proliferation of stem cells |
| CN106754715A (zh) * | 2017-02-13 | 2017-05-31 | 四川新生命干细胞科技股份有限公司 | 一种用于造血干细胞培养的滋养层的制备方法 |
| CN106754652B (zh) * | 2017-03-06 | 2019-04-02 | 广州润虹医药科技股份有限公司 | iPS细胞分化成外胚层祖细胞的无血清诱导培养基及诱导方法 |
| CN106754657B (zh) * | 2017-03-28 | 2022-07-22 | 北京赛斯达生物技术有限公司 | 一种猴胚胎干细胞的无血清培养基 |
| US20200332263A1 (en) * | 2017-05-31 | 2020-10-22 | Promocell Gmbh | Culture medium for pluripotent stem cells |
| CN110157662A (zh) * | 2018-02-15 | 2019-08-23 | 郭永珍 | 一种细胞添加剂及其制备方法 |
| KR102147780B1 (ko) * | 2018-03-16 | 2020-08-25 | 사회복지법인 삼성생명공익재단 | Fgf-17을 유효 성분으로 포함하는 줄기세포 증식 촉진용 배지 조성물 및 이를 이용한 줄기세포 증식 촉진 방법 |
| EP3816279A4 (en) * | 2018-06-27 | 2022-04-20 | Ajinomoto Co., Inc. | ADDITIVE FOR CULTIVATION OF STEM CELLS, CULTURE MEDIUM AND CULTIVATION PROCEDURE |
| CN110684716A (zh) * | 2018-07-08 | 2020-01-14 | 郭永珍 | 一种细胞添加剂的制备方法及其产品 |
| CN109486766B (zh) * | 2018-11-26 | 2021-05-07 | 中山大学 | 一种泪腺干细胞、泪腺干细胞的培养体系和培养方法 |
| CN111484970B (zh) * | 2020-04-30 | 2022-09-16 | 广州再生医学与健康广东省实验室 | 一种低蛋白含量的无血清无饲养层胚胎与多能干细胞培养基 |
| WO2023150293A2 (en) * | 2022-02-03 | 2023-08-10 | Steakholder Foods Ltd. | Accelerated myotube formation |
| CN116210647A (zh) * | 2023-02-03 | 2023-06-06 | 威奥福生物科技(宁波)有限公司 | 一种使用原始生殖细胞干细胞系扩繁白羽肉鸡的方法 |
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| JP2001508302A (ja) * | 1997-01-10 | 2001-06-26 | ライフ テクノロジーズ，インコーポレイテッド | 胚性幹細胞血清置換 |
| US7439064B2 (en) * | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| WO2003029443A1 (en) * | 2001-09-28 | 2003-04-10 | Es Cell International Pte Ltd | Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers |
| WO2004111210A1 (en) * | 2003-06-12 | 2004-12-23 | Yeda Research & Development Co.Ltd | Enhancement of oligodendrocyte differentiation |
| CA2552229C (en) * | 2003-12-26 | 2013-03-12 | Miho Furue | Basal medium for es cell culturing |
| EP1809739B1 (en) * | 2004-07-13 | 2014-10-15 | Asterias Biotherapeutics, Inc. | Medium for growing human embryonic stem cells |
| JP5420837B2 (ja) * | 2004-09-08 | 2014-02-19 | ウィスコンシン アラムニ リサーチ ファンデーション | 胚幹細胞の培地及び培養 |
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- 2007-07-10 JP JP2009518958A patent/JP5227318B2/ja active Active
- 2007-07-10 CA CA002657539A patent/CA2657539A1/en not_active Abandoned
- 2007-07-10 EP EP07766180A patent/EP2038404A2/en not_active Withdrawn
- 2007-07-10 CN CNA2007800249934A patent/CN101490243A/zh active Pending
- 2007-07-10 WO PCT/GB2007/002584 patent/WO2008007082A2/en active Application Filing
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Also Published As
| Publication number | Publication date |
|---|---|
| WO2008007082A2 (en) | 2008-01-17 |
| WO2008007082A3 (en) | 2008-03-06 |
| GB2452456A (en) | 2009-03-04 |
| JP2009542247A (ja) | 2009-12-03 |
| AU2007274065A1 (en) | 2008-01-17 |
| GB0613756D0 (en) | 2006-08-23 |
| GB0823575D0 (en) | 2009-01-28 |
| EP2038404A2 (en) | 2009-03-25 |
| CN101490243A (zh) | 2009-07-22 |
| CA2657539A1 (en) | 2008-01-17 |
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