JP2009521232A5 - - Google Patents

Download PDF

Info

Publication number
JP2009521232A5
JP2009521232A5 JP2008547733A JP2008547733A JP2009521232A5 JP 2009521232 A5 JP2009521232 A5 JP 2009521232A5 JP 2008547733 A JP2008547733 A JP 2008547733A JP 2008547733 A JP2008547733 A JP 2008547733A JP 2009521232 A5 JP2009521232 A5 JP 2009521232A5
Authority
JP
Japan
Prior art keywords
vegf
support
buffer solution
chromatography support
hydrophobic interaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2008547733A
Other languages
English (en)
Japanese (ja)
Other versions
JP5036727B2 (ja
JP2009521232A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2006/062320 external-priority patent/WO2007130154A2/en
Publication of JP2009521232A publication Critical patent/JP2009521232A/ja
Publication of JP2009521232A5 publication Critical patent/JP2009521232A5/ja
Application granted granted Critical
Publication of JP5036727B2 publication Critical patent/JP5036727B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2008547733A 2005-12-22 2006-12-19 ヘパリン結合タンパク質の組み換え体産生 Active JP5036727B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US75361505P 2005-12-22 2005-12-22
US60/753,615 2005-12-22
US80743206P 2006-07-14 2006-07-14
US60/807,432 2006-07-14
PCT/US2006/062320 WO2007130154A2 (en) 2005-12-22 2006-12-19 Recombinant production of heparin binding proteins

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2011174491A Division JP2011254831A (ja) 2005-12-22 2011-08-10 ヘパリン結合タンパク質の組み換え体産生

Publications (3)

Publication Number Publication Date
JP2009521232A JP2009521232A (ja) 2009-06-04
JP2009521232A5 true JP2009521232A5 (https=) 2011-09-29
JP5036727B2 JP5036727B2 (ja) 2012-09-26

Family

ID=38668202

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2008547733A Active JP5036727B2 (ja) 2005-12-22 2006-12-19 ヘパリン結合タンパク質の組み換え体産生
JP2011174491A Pending JP2011254831A (ja) 2005-12-22 2011-08-10 ヘパリン結合タンパク質の組み換え体産生

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2011174491A Pending JP2011254831A (ja) 2005-12-22 2011-08-10 ヘパリン結合タンパク質の組み換え体産生

Country Status (16)

Country Link
US (3) US20080032343A1 (https=)
EP (2) EP2336164A1 (https=)
JP (2) JP5036727B2 (https=)
KR (1) KR101067525B1 (https=)
AR (1) AR058625A1 (https=)
AT (1) ATE497973T1 (https=)
AU (1) AU2006343279B2 (https=)
BR (1) BRPI0621124A2 (https=)
CA (1) CA2633554C (https=)
DE (1) DE602007012412D1 (https=)
IL (1) IL191968A (https=)
MY (1) MY143274A (https=)
NZ (1) NZ568809A (https=)
RU (1) RU2385877C1 (https=)
TW (1) TWI369362B (https=)
WO (1) WO2007130154A2 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ568809A (en) 2005-12-22 2011-08-26 Genentech Inc Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents
US20080125580A1 (en) 2006-07-14 2008-05-29 Genentech, Inc. Refolding of Recombinant Proteins
CA2742817A1 (en) 2008-11-20 2010-05-27 Biogen Idec Ma Inc. Arginine inactivation of viruses
EA020621B1 (ru) 2009-06-22 2014-12-30 Амген Инк. Рефолдинг белков с использованием химически контролируемого окислительно-восстановительного состояния
LT3660032T (lt) 2009-06-25 2026-02-25 Amgen Inc. Ne žinduolių sistemoje ekspresuotų baltymų surišamojo gryninimo būdai
TWI662176B (zh) 2010-02-25 2019-06-11 約翰 克蘭曼特 普瑞斯頓 鷹架
KR102283127B1 (ko) * 2018-06-19 2021-07-29 주식회사 엠디헬스케어 류코노스톡속 균주를 포함하는 간 기능 개선용 조성물
US11180540B2 (en) * 2019-12-06 2021-11-23 Regeneron Pharmaceuticals, Inc. Anti-VEGF protein compositions and methods for producing the same
CN117069810A (zh) * 2022-05-09 2023-11-17 上海科技大学 一种膜蛋白的纯化方法

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4169950A (en) * 1977-12-08 1979-10-02 Research Organics Amino-hydroxy-alkyl sulfonic acid zwitterions
US4263428A (en) 1978-03-24 1981-04-21 The Regents Of The University Of California Bis-anthracycline nucleic acid function inhibitors and improved method for administering the same
US4565785A (en) 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
ZA811368B (en) 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
EP0052322B1 (de) 1980-11-10 1985-03-27 Gersonde, Klaus, Prof. Dr. Verfahren zur Herstellung von Lipid-Vesikeln durch Ultraschallbehandlung, Anwendung des Verfahrens und Vorrichtung zur Durchführung des Verfahrens
US4485045A (en) 1981-07-06 1984-11-27 Research Corporation Synthetic phosphatidyl cholines useful in forming liposomes
JPS58118008A (ja) 1982-01-06 1983-07-13 Nec Corp デ−タ処理装置
DE3374837D1 (en) 1982-02-17 1988-01-21 Ciba Geigy Ag Lipids in the aqueous phase
DE3218121A1 (de) 1982-05-14 1983-11-17 Leskovar, Peter, Dr.-Ing., 8000 München Arzneimittel zur tumorbehandlung
EP0102324A3 (de) 1982-07-29 1984-11-07 Ciba-Geigy Ag Lipide und Tenside in wässriger Phase
US4673641A (en) 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4544545A (en) 1983-06-20 1985-10-01 Trustees University Of Massachusetts Liposomes containing modified cholesterol for organ targeting
US4710473A (en) 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
DE3483949D1 (de) 1983-09-26 1991-02-21 Udo Dr Med Ehrenfeld Mittel und erzeugnis fuer die diagnose und therapie von tumoren sowie zur behandlung von schwaechen der zelligen und humoralen immunabwehr.
EP0143949B1 (en) 1983-11-01 1988-10-12 TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION Pharmaceutical composition containing urokinase
US4795706A (en) * 1985-01-31 1989-01-03 Eli Lilly And Company Novel expression control sequences
ES8800957A1 (es) 1985-02-22 1987-12-01 Monsanto Co Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
AU614137B2 (en) 1988-06-06 1991-08-22 Takeda Chemical Industries Ltd. Stabilized fgf composition and production thereof
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5332671A (en) * 1989-05-12 1994-07-26 Genetech, Inc. Production of vascular endothelial cell growth factor and DNA encoding same
US5194596A (en) 1989-07-27 1993-03-16 California Biotechnology Inc. Production of vascular endothelial cell growth factor
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5304472A (en) 1992-11-20 1994-04-19 Genentech, Inc. Method of controlling polypeptide production in bacterial cells
US5342763A (en) 1992-11-23 1994-08-30 Genentech, Inc. Method for producing polypeptide via bacterial fermentation
WO1994012158A1 (en) 1992-12-02 1994-06-09 Alkermes Controlled Therapeutics, Inc. Controlled release growth hormone containing microspheres
US5331095A (en) 1993-04-12 1994-07-19 Scios Nova Inc. Process for purification of basic fibroblast growth factor
JP4098372B2 (ja) * 1993-07-28 2008-06-11 三菱化学株式会社 ヘパリン結合性増殖因子の生産方法
US5663304A (en) 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
US5464815A (en) 1993-09-08 1995-11-07 Genentech, Inc. Inhibition of heparin-binding
ES2249762T3 (es) * 1994-03-08 2006-04-01 Human Genome Sciences, Inc. Factor de crecimiento del endotelio vascular 2.
RU2200573C2 (ru) * 1994-04-21 2003-03-20 Леукотех А/С Связывающий гепарин белок для лечения сепсиса
DK0779806T3 (da) 1994-09-09 2000-11-27 Takeda Chemical Industries Ltd Præparat til forsinket frigivelse indeholdende et metalsalt af et peptid
FR2729972B1 (fr) * 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
NZ310644A (en) 1995-06-07 1999-08-30 Alkermes Inc Composition for sustained release of human growth hormone
ZA965368B (en) 1995-07-14 1997-01-14 Novo Nordisk As A pharmaceutical formulation
US6750044B1 (en) 1996-10-17 2004-06-15 Genentech, Inc. Variants of vascular endothelial cell growth factor having antagonistic properties, nucleic acids encoding the same and host cells comprising those nucleic acids
US6632425B1 (en) * 1997-03-20 2003-10-14 Human Genome Sciences, Inc. Chemokine compositions
DE19735711C2 (de) 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
US6653098B1 (en) 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE69840035D1 (de) 1998-03-31 2008-10-30 Tonghua Gantech Biotechnology Chimäres protein welches eine intramolekulare chap zur insulinproduktion
EP1500661A1 (en) 1998-06-01 2005-01-26 Genetech, Inc. Separation of protein monomers from aggregates by use of ion-exchange chromatography
US6180367B1 (en) 1998-10-28 2001-01-30 Genentech, Inc. Process for bacterial production of polypeptides
US6783953B1 (en) * 1998-12-22 2004-08-31 Janssen Pharmaceutica N.V. Vascular endothelial growth factor-X
CA2374050A1 (en) * 1999-05-20 2000-11-30 Scios Inc. Vascular endothelial growth factor dimers
US6583268B2 (en) 2000-01-25 2003-06-24 Oklahoma Medical Research Foundation Universal procedure for refolding recombinant proteins
AUPQ568100A0 (en) * 2000-02-16 2000-03-09 Amrad Operations Pty. Limited A method for producing recombinant molecules
AU2001244647A1 (en) * 2000-03-30 2001-10-15 Takeda Chemical Industries Ltd. Process for producing recombinant protein
IL152804A0 (en) 2000-05-16 2003-06-24 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
US7611711B2 (en) * 2001-01-17 2009-11-03 Vegenics Limited VEGFR-3 inhibitor materials and methods
JP2005505494A (ja) 2001-02-23 2005-02-24 イミュネックス・コーポレーション 活性タンパク質の回収増加
WO2003094617A2 (en) 2002-05-06 2003-11-20 Genentech, Inc. Use of vegf for treating bone defects
JP5111729B2 (ja) 2002-06-05 2013-01-09 ジェネンテック, インコーポレイテッド 肝成長及び肝保護のための組成物と方法
JP2006517415A (ja) 2003-01-09 2006-07-27 ジェネンテック・インコーポレーテッド ポリペプチドの精製
EP1449848A1 (en) 2003-02-20 2004-08-25 GBF German Research Centre for Biotechnology Method for the production of cystine-knot proteins
CN1890371A (zh) 2003-12-10 2007-01-03 伊莱利利公司 成纤维细胞生长因子的21突变蛋白
DE102004015965A1 (de) 2004-04-01 2005-10-20 Aventis Pharma Gmbh Verfahren zur Gewinnung von Insulinen durch verbesserte Luftbegasung der Faltung
JP4940136B2 (ja) 2004-06-28 2012-05-30 イエダ リサーチ アンド ディベロップメント カンパニー リミテッド キメラタンパク質およびその使用
AU2005306168A1 (en) 2004-11-22 2006-05-26 Anaphore, Inc. TNF antagonists
DE602006003108D1 (de) * 2005-03-03 2008-11-27 Ajinomoto Kk Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
NZ568809A (en) 2005-12-22 2011-08-26 Genentech Inc Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents

Similar Documents

Publication Publication Date Title
Clark Protein refolding for industrial processes
US5728804A (en) Use of cyclodextrins for protein renaturation
Qoronfleh et al. Confronting high-throughput protein refolding using high pressure and solution screens
De Bernardez Clark et al. Oxidative renaturation of hen egg‐white lysozyme. Folding vs aggregation
Batas et al. Protein refolding at high concentration using size‐exclusion chromatography
JP3646195B2 (ja) ポリペプチドの水性多相単離
JP3648242B2 (ja) 組み換えインスリン様成長因子igf−i様ポリペプチドの再折りたたみ
Jungbauer et al. Current status of technical protein refolding
US8710197B2 (en) High pressure refolding of protein aggregates and inclusion bodies
IE61756B1 (en) Purification and activation of proteins from insoluble inclusion bodies
US4656255A (en) Protein recovery
JP2009521232A5 (https=)
Hagen et al. Protein refolding in reversed micelles: Interactions of the protein with micelle components
Gautam et al. Non-chromatographic strategies for protein refolding
CN105732820B (zh) 一种重组人胰岛素原融合蛋白的复性方法
Khodarahmi et al. Refolding of chemically denatured α-amylase in dilution additive mode
JP5036727B2 (ja) ヘパリン結合タンパク質の組み換え体産生
JP3930051B2 (ja) 正しく折畳まれた生物学的に活性の組換えタンパク質の製造方法
MATSUBARA et al. " Loose folding" and" delayed oxidation" procedures successfully applied for refolding of fully reduced hen egg white lysozyme
US20180037604A1 (en) Process for the purification of recombinant proteins
CN1220703C (zh) 在水性溶剂中贮存蛋白质期间稳定复杂混合物中的蛋白质的方法
Nian et al. Chaperone-assisted column refolding of gloshedobin with the use of refolding cocktail
Luo et al. On-column refolding of denatured lysozyme by the conjoint chromatography composed of SEC and immobilized recombinant DsbA
Jamaluddin et al. On-column refolding of recombinant fungal endoglucanase
Chien et al. Refolding of recombinant N-acetyl-d-glucosamine 2-epimerase by a fed-batch process