AR058625A1 - Produccion recombinante de proteinas de union a heparina - Google Patents

Produccion recombinante de proteinas de union a heparina

Info

Publication number
AR058625A1
AR058625A1 ARP060105732A ARP060105732A AR058625A1 AR 058625 A1 AR058625 A1 AR 058625A1 AR P060105732 A ARP060105732 A AR P060105732A AR P060105732 A ARP060105732 A AR P060105732A AR 058625 A1 AR058625 A1 AR 058625A1
Authority
AR
Argentina
Prior art keywords
heparin binding
binding protein
heparin
agent
renatured
Prior art date
Application number
ARP060105732A
Other languages
English (en)
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Priority to TW095148610A priority Critical patent/TWI369362B/zh
Publication of AR058625A1 publication Critical patent/AR058625A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un proceso para recuperar y purificar las proteínas de union a heparina renaturalizadas producidas en células hospedantes heterologas que incluye el paso de incubacion de la proteína solubilizada con una especie polianionica tal como sulfato de dextrano. Reivindicacion 1: Un proceso para recuperar una proteína de union a heparina a partir de un cultivo de células procariotas, el proceso que comprende los pasos de: (a) aislar dicha proteína de union a heparina del periplasma de dicho cultivo de células procariotas; (b) desnaturalizar dicha proteína de union a heparina en una primera solucion buferizada que comprende un agente caotropico y un agente reductor; (c) incubar dicha proteína de union a heparina desnaturalizada en una segunda solucion buferizada que comprende un agente caotropico y un agente polianionico sulfatado durante tal período de tiempo y en tales condiciones que ocurra la renaturalizacion de la proteína de union a heparina, y (d) recuperar dicha proteína de union a heparina renaturalizada, donde hay aproximadamente un aumento de 2 a 5 veces de la proteína de union a heparina renaturalizada recuperada en comparacion con la incubacion sin un agente polianionico no sulfatado. Reivindicacion 2: El proceso de la reivindicacion 1, donde la proteína de union a heparina es un factor de crecimiento de union a heparina. Reivindicacion 3: El proceso de la reivindicacion 2, donde el factor de crecimiento de union a heparina es el factor de crecimiento endotelial vascular (VEGF).
ARP060105732A 2005-12-22 2006-12-21 Produccion recombinante de proteinas de union a heparina AR058625A1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW095148610A TWI369362B (en) 2005-12-22 2006-12-22 Recombinant production of heparin binding proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75361505P 2005-12-22 2005-12-22
US80743206P 2006-07-14 2006-07-14

Publications (1)

Publication Number Publication Date
AR058625A1 true AR058625A1 (es) 2008-02-13

Family

ID=38668202

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP060105732A AR058625A1 (es) 2005-12-22 2006-12-21 Produccion recombinante de proteinas de union a heparina

Country Status (17)

Country Link
US (3) US20080032343A1 (es)
EP (2) EP1973942B1 (es)
JP (2) JP5036727B2 (es)
KR (1) KR101067525B1 (es)
AR (1) AR058625A1 (es)
AT (1) ATE497973T1 (es)
AU (1) AU2006343279B2 (es)
BR (1) BRPI0621124A2 (es)
CA (1) CA2633554C (es)
DE (1) DE602007012412D1 (es)
HK (1) HK1119188A1 (es)
IL (1) IL191968A (es)
MY (1) MY143274A (es)
NZ (1) NZ568809A (es)
RU (1) RU2385877C1 (es)
TW (1) TWI369362B (es)
WO (1) WO2007130154A2 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9994612B2 (en) 2006-07-14 2018-06-12 Genentech, Inc. Refolding of recombinant proteins

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NZ568809A (en) 2005-12-22 2011-08-26 Genentech Inc Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents
DK2350271T3 (en) 2008-11-20 2016-04-04 Biogen Ma Inc ARGININ INACTIVATION OF ENVIRONMENT VIRA
BRPI1011940B8 (pt) 2009-06-22 2021-08-03 Amgen Inc método de redobramento de uma proteína expressa em um sistema de expressão de não mamífero
EP2445924B2 (en) 2009-06-25 2023-12-13 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9994612B2 (en) 2006-07-14 2018-06-12 Genentech, Inc. Refolding of recombinant proteins

Also Published As

Publication number Publication date
US8906648B2 (en) 2014-12-09
IL191968A0 (en) 2008-12-29
US20100261888A1 (en) 2010-10-14
RU2385877C1 (ru) 2010-04-10
EP2336164A1 (en) 2011-06-22
WO2007130154A3 (en) 2008-06-26
NZ568809A (en) 2011-08-26
JP2011254831A (ja) 2011-12-22
ATE497973T1 (de) 2011-02-15
EP1973942A2 (en) 2008-10-01
US20080032343A1 (en) 2008-02-07
CA2633554C (en) 2012-11-13
BRPI0621124A2 (pt) 2011-11-29
KR20080087128A (ko) 2008-09-30
JP2009521232A (ja) 2009-06-04
IL191968A (en) 2013-06-27
US20130217867A1 (en) 2013-08-22
HK1119188A1 (en) 2009-07-17
WO2007130154A8 (en) 2008-04-17
TW200734351A (en) 2007-09-16
AU2006343279A1 (en) 2007-11-15
RU2008130072A (ru) 2010-01-27
CA2633554A1 (en) 2007-11-15
WO2007130154A2 (en) 2007-11-15
DE602007012412D1 (de) 2011-03-24
AU2006343279B2 (en) 2012-03-29
TWI369362B (en) 2012-08-01
JP5036727B2 (ja) 2012-09-26
MY143274A (en) 2011-04-15
KR101067525B1 (ko) 2011-09-27
EP1973942B1 (en) 2011-02-09

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