DE69840035D1 - Chimäres protein welches eine intramolekulare chap zur insulinproduktion - Google Patents
Chimäres protein welches eine intramolekulare chap zur insulinproduktionInfo
- Publication number
- DE69840035D1 DE69840035D1 DE69840035T DE69840035T DE69840035D1 DE 69840035 D1 DE69840035 D1 DE 69840035D1 DE 69840035 T DE69840035 T DE 69840035T DE 69840035 T DE69840035 T DE 69840035T DE 69840035 D1 DE69840035 D1 DE 69840035D1
- Authority
- DE
- Germany
- Prior art keywords
- chimeric protein
- insulin
- intramolecular
- imc
- chap
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 10
- 102000004877 Insulin Human genes 0.000 title abstract 5
- 108090001061 Insulin Proteins 0.000 title abstract 5
- 102000037865 fusion proteins Human genes 0.000 title abstract 5
- 108020001507 fusion proteins Proteins 0.000 title abstract 5
- 229940125396 insulin Drugs 0.000 title abstract 5
- 239000002243 precursor Substances 0.000 abstract 4
- 108010006519 Molecular Chaperones Proteins 0.000 abstract 1
- 150000001413 amino acids Chemical group 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 239000012752 auxiliary agent Substances 0.000 abstract 1
- 230000003196 chaotropic effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Diabetes (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN1998/000052 WO1999050302A1 (en) | 1998-03-31 | 1998-03-31 | Chimeric protein containing an intramolecular chaperone-like sequence and its application to insulin production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE69840035D1 true DE69840035D1 (de) | 2008-10-30 |
Family
ID=4575041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69840035T Expired - Lifetime DE69840035D1 (de) | 1998-03-31 | 1998-03-31 | Chimäres protein welches eine intramolekulare chap zur insulinproduktion |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US6924120B1 (de) |
| EP (1) | EP1066328B1 (de) |
| JP (1) | JP4472870B2 (de) |
| KR (1) | KR100554490B1 (de) |
| CN (1) | CN1197876C (de) |
| AT (1) | ATE408631T1 (de) |
| AU (1) | AU765574B2 (de) |
| BR (1) | BR9815788B1 (de) |
| CA (1) | CA2324513C (de) |
| DE (1) | DE69840035D1 (de) |
| DK (1) | DK1066328T3 (de) |
| ES (1) | ES2317665T3 (de) |
| IL (1) | IL138658A0 (de) |
| MX (1) | MXPA00009564A (de) |
| WO (1) | WO1999050302A1 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2372199A1 (en) * | 1999-05-14 | 2000-11-23 | Medical Research Council | Oligomeric chaperone proteins |
| EP1179013A1 (de) * | 1999-05-14 | 2002-02-13 | Medical Research Council | Proteingerüst und seine verwendung zur multimerisierung von monomeren peptiden |
| GB9913437D0 (en) * | 1999-06-09 | 1999-08-11 | Medical Res Council | Fusion proteins |
| US20020164712A1 (en) * | 2000-12-11 | 2002-11-07 | Tonghua Gantech Biotechnology Ltd. | Chimeric protein containing an intramolecular chaperone-like sequence |
| DE602007012412D1 (de) | 2005-12-22 | 2011-03-24 | Genentech Inc | Rekombinante produktion heparinbindender proteine |
| WO2008008975A2 (en) * | 2006-07-14 | 2008-01-17 | Genentech, Inc. | Refolding of recombinant proteins |
| US7951559B2 (en) * | 2007-07-25 | 2011-05-31 | E.I. Du Pont De Nemours And Company | Recombinant peptide production using a cross-linkable solubility tag |
| RU2447149C1 (ru) * | 2011-03-24 | 2012-04-10 | Винсорт Менеджемент Инк | РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pMSIN4, КОДИРУЮЩАЯ ГИБРИДНЫЙ ПОЛИПЕПТИД - ПРЕДШЕСТВЕННИК ИНСУЛИНА ЧЕЛОВЕКА, ШТАММ BL21(DE3)/pMSIN4-ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ИНСУЛИНА ЧЕЛОВЕКА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОГО ИНСУЛИНА ЧЕЛОВЕКА |
| CN102628072B (zh) * | 2012-03-20 | 2013-12-04 | 甘李药业股份有限公司 | 一种胰岛素的制备方法 |
| CN103439512B (zh) * | 2013-07-30 | 2014-05-07 | 通化东宝药业股份有限公司 | 一种检测特异性重组人胰岛素大肠杆菌残留宿主蛋白的方法 |
| CN112142848A (zh) * | 2019-06-26 | 2020-12-29 | 中国科学院大连化学物理研究所 | 一种重组人胰岛素及其纯化制备方法 |
| CN114805544B (zh) * | 2022-06-23 | 2022-09-09 | 北京惠之衡生物科技有限公司 | 一种赖脯胰岛素前体、其重组基因工程菌及其构建方法 |
| CN114805610B (zh) * | 2022-06-23 | 2022-10-04 | 北京惠之衡生物科技有限公司 | 一种高表达甘精胰岛素前体的重组基因工程菌及其构建方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4342832A (en) | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| NZ199391A (en) | 1981-01-02 | 1985-12-13 | Genentech Inc | Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin |
| US5254463A (en) | 1981-09-18 | 1993-10-19 | Genentech, Inc. | Method for expression of bovine growth hormone |
| US4665160A (en) * | 1982-03-22 | 1987-05-12 | Genentech, Inc. | Novel human growth hormone like protein HGH-V encoded in the human genome |
| DK58285D0 (da) * | 1984-05-30 | 1985-02-08 | Novo Industri As | Peptider samt fremstilling og anvendelse deraf |
| US5191063A (en) | 1989-05-02 | 1993-03-02 | University Of Medicine And Dentistry Of New Jersey | Production of biologically active polypeptides by treatment with an exogenous peptide sequence |
| US5358857A (en) | 1989-08-29 | 1994-10-25 | The General Hospital Corp. | Method of preparing fusion proteins |
| US5422110A (en) | 1991-10-16 | 1995-06-06 | University Of Saskatchewan | Enhanced immunogenicity using leukotoxin chimeras |
| ES2097426T3 (es) | 1992-12-02 | 1997-04-01 | Hoechst Ag | Procedimiento para la obtencion de proinsulina con puentes de cistina correctamente unidos. |
| US6316004B1 (en) | 1993-06-22 | 2001-11-13 | T. Tikhonenko | Chimeric somatostatin containing protein and encoding DNA, plasmids of expression, method for preparing chimeric protein, strain-producers, immunogenic composition, method for increasing the productivity of farm animals |
| US6001604A (en) | 1993-12-29 | 1999-12-14 | Bio-Technology General Corp. | Refolding of proinsulins without addition of reducing agents |
| DE69434909T3 (de) * | 1994-12-29 | 2012-02-09 | Ferring International Center S.A. | Herstellung von humaninsulin |
| AU4658596A (en) * | 1995-02-03 | 1996-08-21 | G.D. Searle & Co. | Novel c-mpl ligands |
| US5559128A (en) | 1995-04-18 | 1996-09-24 | Merck & Co., Inc. | 3-substituted piperidines promote release of growth hormone |
| SE9504019D0 (sv) | 1995-11-13 | 1995-11-13 | Pharmacia Ab | Method for production of proteins |
| AUPO394896A0 (en) | 1996-11-29 | 1997-01-02 | May, Peter Andrew John | Hose mounting bracket |
| US20020164712A1 (en) * | 2000-12-11 | 2002-11-07 | Tonghua Gantech Biotechnology Ltd. | Chimeric protein containing an intramolecular chaperone-like sequence |
| WO2002079251A2 (en) * | 2001-04-02 | 2002-10-10 | Novo Nordisk A/S | Method for making human insulin precursors |
-
1998
- 1998-03-31 WO PCT/CN1998/000052 patent/WO1999050302A1/en active IP Right Grant
- 1998-03-31 CN CNB988139413A patent/CN1197876C/zh not_active Expired - Lifetime
- 1998-03-31 BR BRPI9815788-4A patent/BR9815788B1/pt not_active IP Right Cessation
- 1998-03-31 US US09/423,100 patent/US6924120B1/en not_active Expired - Fee Related
- 1998-03-31 KR KR1020007010951A patent/KR100554490B1/ko not_active IP Right Cessation
- 1998-03-31 IL IL13865898A patent/IL138658A0/xx unknown
- 1998-03-31 ES ES98912192T patent/ES2317665T3/es not_active Expired - Lifetime
- 1998-03-31 MX MXPA00009564A patent/MXPA00009564A/es unknown
- 1998-03-31 CA CA2324513A patent/CA2324513C/en not_active Expired - Fee Related
- 1998-03-31 EP EP98912192A patent/EP1066328B1/de not_active Expired - Lifetime
- 1998-03-31 AT AT98912192T patent/ATE408631T1/de active
- 1998-03-31 JP JP2000541204A patent/JP4472870B2/ja not_active Expired - Fee Related
- 1998-03-31 AU AU67164/98A patent/AU765574B2/en not_active Ceased
- 1998-03-31 DE DE69840035T patent/DE69840035D1/de not_active Expired - Lifetime
- 1998-03-31 DK DK98912192T patent/DK1066328T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999050302A1 (en) | 1999-10-07 |
| CN1197876C (zh) | 2005-04-20 |
| ATE408631T1 (de) | 2008-10-15 |
| EP1066328A1 (de) | 2001-01-10 |
| JP4472870B2 (ja) | 2010-06-02 |
| AU765574B2 (en) | 2003-09-25 |
| CA2324513C (en) | 2013-05-28 |
| EP1066328B1 (de) | 2008-09-17 |
| CA2324513A1 (en) | 1999-10-07 |
| JP2004505601A (ja) | 2004-02-26 |
| DK1066328T3 (da) | 2009-02-09 |
| BR9815788A (pt) | 2000-11-28 |
| KR20010042383A (ko) | 2001-05-25 |
| US6924120B1 (en) | 2005-08-02 |
| KR100554490B1 (ko) | 2006-03-03 |
| CN1291199A (zh) | 2001-04-11 |
| ES2317665T3 (es) | 2009-04-16 |
| AU6716498A (en) | 1999-10-18 |
| MXPA00009564A (es) | 2004-03-10 |
| BR9815788B1 (pt) | 2013-06-04 |
| EP1066328A4 (de) | 2002-06-05 |
| IL138658A0 (en) | 2001-10-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |