JP2009082138A5 - - Google Patents
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- JP2009082138A5 JP2009082138A5 JP2008301520A JP2008301520A JP2009082138A5 JP 2009082138 A5 JP2009082138 A5 JP 2009082138A5 JP 2008301520 A JP2008301520 A JP 2008301520A JP 2008301520 A JP2008301520 A JP 2008301520A JP 2009082138 A5 JP2009082138 A5 JP 2009082138A5
- Authority
- JP
- Japan
- Prior art keywords
- temperature
- medium
- hmba
- cell line
- days
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 16
- BNQSTAOJRULKNX-UHFFFAOYSA-N N-(6-acetamidohexyl)acetamide Chemical compound CC(=O)NCCCCCCNC(C)=O BNQSTAOJRULKNX-UHFFFAOYSA-N 0.000 claims 10
- 229920001184 polypeptide Polymers 0.000 claims 8
- 102000004196 processed proteins & peptides Human genes 0.000 claims 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims 8
- 239000002609 medium Substances 0.000 claims 6
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical group CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 claims 4
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 claims 3
- 150000004652 butanoic acids Chemical class 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 3
- 210000004962 mammalian cell Anatomy 0.000 claims 3
- 108010029697 CD40 Ligand Proteins 0.000 claims 2
- 102100032937 CD40 ligand Human genes 0.000 claims 2
- 102000003951 Erythropoietin Human genes 0.000 claims 2
- 108010075944 Erythropoietin Receptors Proteins 0.000 claims 2
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 claims 2
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 claims 2
- 101100369992 Homo sapiens TNFSF10 gene Proteins 0.000 claims 2
- 102000019223 Interleukin-1 receptor Human genes 0.000 claims 2
- 108050006617 Interleukin-1 receptor Proteins 0.000 claims 2
- 102000010787 Interleukin-4 Receptors Human genes 0.000 claims 2
- 108010038486 Interleukin-4 Receptors Proteins 0.000 claims 2
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 claims 2
- 108700012411 TNFSF10 Proteins 0.000 claims 2
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 claims 2
- 229960001948 caffeine Drugs 0.000 claims 2
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 2
- 229940105423 erythropoietin Drugs 0.000 claims 2
- 108700014844 flt3 ligand Proteins 0.000 claims 2
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 claims 2
- 102000003298 tumor necrosis factor receptor Human genes 0.000 claims 2
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 claims 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims 1
- 239000004020 conductor Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 210000004408 hybridoma Anatomy 0.000 claims 1
- 239000013587 production medium Substances 0.000 claims 1
- 239000012679 serum free medium Substances 0.000 claims 1
- 229940075420 xanthine Drugs 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36824802P | 2002-03-27 | 2002-03-27 | |
| US36824602P | 2002-03-27 | 2002-03-27 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003580502A Division JP2005521401A (ja) | 2002-03-27 | 2003-03-27 | ポリペプチド産生を増加させる方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009082138A JP2009082138A (ja) | 2009-04-23 |
| JP2009082138A5 true JP2009082138A5 (enExample) | 2009-06-04 |
| JP4414472B2 JP4414472B2 (ja) | 2010-02-10 |
Family
ID=28678222
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003580502A Pending JP2005521401A (ja) | 2002-03-27 | 2003-03-27 | ポリペプチド産生を増加させる方法 |
| JP2008024395A Expired - Lifetime JP4414466B2 (ja) | 2002-03-27 | 2008-02-04 | ポリペプチド産生を増加させる方法 |
| JP2008301520A Expired - Lifetime JP4414472B2 (ja) | 2002-03-27 | 2008-11-26 | ポリペプチド産生を増加させる方法 |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003580502A Pending JP2005521401A (ja) | 2002-03-27 | 2003-03-27 | ポリペプチド産生を増加させる方法 |
| JP2008024395A Expired - Lifetime JP4414466B2 (ja) | 2002-03-27 | 2008-02-04 | ポリペプチド産生を増加させる方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US6872549B2 (enExample) |
| EP (1) | EP1497444B1 (enExample) |
| JP (3) | JP2005521401A (enExample) |
| AU (1) | AU2003220529B2 (enExample) |
| CA (1) | CA2480121C (enExample) |
| ES (1) | ES2557741T3 (enExample) |
| MX (1) | MXPA04009381A (enExample) |
| PL (1) | PL376821A1 (enExample) |
| WO (1) | WO2003083066A2 (enExample) |
Families Citing this family (46)
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| US20050005310A1 (en) * | 1999-07-12 | 2005-01-06 | Genentech, Inc. | Expression vectors and methods |
| WO2003083066A2 (en) * | 2002-03-27 | 2003-10-09 | Immunex Corporation | Methods for increasing polypeptide production |
| AU2003298674A1 (en) * | 2002-11-14 | 2004-06-15 | Genentech Inc | Intron fusion construct and method of using for selecting high-expressing production cell lines |
| JP2007525176A (ja) * | 2003-04-25 | 2007-09-06 | イミュネックス・コーポレーション | 組換えタンパク質発現の誘導剤 |
| US20050112194A1 (en) * | 2003-08-21 | 2005-05-26 | Duchesnay Inc. | Micronutrient supplement dispensing package |
| JP5349752B2 (ja) * | 2003-08-21 | 2013-11-20 | デュシェネ インク | 微量栄養素サプリメント |
| EP1697414A2 (en) * | 2003-12-23 | 2006-09-06 | Applied Research Systems ARS Holding N.V. | Process for the production of tumor necrosis factor-binding proteins |
| EP1909831A4 (en) | 2005-06-14 | 2013-02-20 | Amgen Inc | SELF-BUFFING PROTEIN FORMULATIONS |
| WO2007049567A1 (ja) * | 2005-10-24 | 2007-05-03 | Kyowa Hakko Kogyo Co., Ltd. | 物質の製造方法 |
| AR058568A1 (es) | 2005-12-20 | 2008-02-13 | Bristol Myers Squibb Co | Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo |
| TWI423986B (zh) | 2005-12-20 | 2014-01-21 | 必治妥美雅史谷比公司 | 組合物及製造組合物之方法 |
| US20070249014A1 (en) | 2006-02-10 | 2007-10-25 | Invitrogen Corporation | Labeling and detection of post translationally modified proteins |
| AU2007339423B2 (en) * | 2006-08-14 | 2012-11-15 | Biocontrol Systems, Inc. | Method for detecting pathogens |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| EP2500413A1 (en) | 2006-09-13 | 2012-09-19 | Abbott Laboratories | Cell culture improvements |
| EP2129787A4 (en) * | 2007-03-28 | 2012-05-02 | Medarex Inc | Methods of gene amplification and expression |
| TW200902708A (en) * | 2007-04-23 | 2009-01-16 | Wyeth Corp | Methods of protein production using anti-senescence compounds |
| US20090023186A1 (en) * | 2007-07-22 | 2009-01-22 | Excellgene Sa | Use of valproic acid for enhancing production of recombinant proteins in mammalian cells |
| US20110014624A1 (en) * | 2008-03-12 | 2011-01-20 | Wyeth Llc | Methods For Identifying Cells Suitable For Large-Scale Production of Recombinant Proteins |
| RU2012127383A (ru) * | 2009-12-02 | 2014-01-10 | Акселерон Фарма Инк. | Композиции и способы для увеличения времени полужизни fc-слитых белков в сыворотке |
| EP2623556A4 (en) | 2010-09-30 | 2015-03-11 | Daikin Ind Ltd | DRIP AND RESIN CONNECTION |
| US20130295613A1 (en) * | 2010-12-28 | 2013-11-07 | Chugai Seiyaku Kabushiki Kaisha | Animal cell culturing method |
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| EP2718328A4 (en) | 2011-06-08 | 2014-12-24 | Acceleron Pharma Inc | COMPOSITIONS AND METHODS FOR INCREASING THE HALF TIME OF SERUM |
| HUE033279T2 (en) | 2011-07-01 | 2017-11-28 | Amgen Inc | Mammalian cell culture |
| EP2653548A1 (de) | 2012-04-17 | 2013-10-23 | Greenovation Biotech GmbH | Verfahren zur Erhöhung der Sekretion rekombinanter Proteine |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| BR112015004467A2 (pt) | 2012-09-02 | 2017-03-21 | Abbvie Inc | método para controlar a heterogeneidade de proteínas |
| CA2905010A1 (en) | 2013-03-12 | 2014-09-18 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
| US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| CN105189736A (zh) * | 2013-03-15 | 2015-12-23 | 斯欧考普控股公司 | 适用于提高胎儿体重增加并增强骨特性的可可碱组合物 |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| AU2017345490B2 (en) | 2016-10-21 | 2022-07-07 | Amgen Inc. | Pharmaceutical formulations and methods of making the same |
| KR102190790B1 (ko) * | 2018-10-19 | 2020-12-15 | 주식회사 프로젠 | 변형된 egf 단백질의 생산 방법 |
| JP6963336B2 (ja) * | 2017-10-23 | 2021-11-05 | プロゲン・シーオー., エルティーディー.Progen Co., Ltd. | 改変ｅｇｆタンパク質、その製造方法及びその使用 |
| EP4267717A1 (en) | 2020-12-22 | 2023-11-01 | Amgen Inc. | Cell culture method |
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| EP0005476B1 (de) | 1978-05-17 | 1981-12-30 | Dr. Karl Thomae GmbH | Verfahren zur Herstellung von Humaninterferon |
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-
2003
- 2003-03-27 WO PCT/US2003/009266 patent/WO2003083066A2/en not_active Ceased
- 2003-03-27 EP EP03716840.8A patent/EP1497444B1/en not_active Expired - Lifetime
- 2003-03-27 ES ES03716840.8T patent/ES2557741T3/es not_active Expired - Lifetime
- 2003-03-27 JP JP2003580502A patent/JP2005521401A/ja active Pending
- 2003-03-27 AU AU2003220529A patent/AU2003220529B2/en not_active Expired
- 2003-03-27 MX MXPA04009381A patent/MXPA04009381A/es active IP Right Grant
- 2003-03-27 CA CA2480121A patent/CA2480121C/en not_active Expired - Fee Related
- 2003-03-27 US US10/400,334 patent/US6872549B2/en not_active Expired - Lifetime
- 2003-03-27 PL PL376821A patent/PL376821A1/pl unknown
-
2005
- 2005-02-04 US US11/051,149 patent/US7452695B2/en not_active Expired - Lifetime
-
2008
- 2008-02-04 JP JP2008024395A patent/JP4414466B2/ja not_active Expired - Lifetime
- 2008-11-26 JP JP2008301520A patent/JP4414472B2/ja not_active Expired - Lifetime
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