JP2008540363A - シュピーゲルマーの新規な使用 - Google Patents
シュピーゲルマーの新規な使用 Download PDFInfo
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- JP2008540363A JP2008540363A JP2008509380A JP2008509380A JP2008540363A JP 2008540363 A JP2008540363 A JP 2008540363A JP 2008509380 A JP2008509380 A JP 2008509380A JP 2008509380 A JP2008509380 A JP 2008509380A JP 2008540363 A JP2008540363 A JP 2008540363A
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| US9492400B2 (en) | 2004-11-04 | 2016-11-15 | Massachusetts Institute Of Technology | Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals |
| WO2007070682A2 (en) | 2005-12-15 | 2007-06-21 | Massachusetts Institute Of Technology | System for screening particles |
| CA2648099C (en) | 2006-03-31 | 2012-05-29 | The Brigham And Women's Hospital, Inc | System for targeted delivery of therapeutic agents |
| EP2019691B1 (en) | 2006-05-15 | 2020-08-12 | Massachusetts Institute of Technology | Polymers for functional particles |
| US9381477B2 (en) | 2006-06-23 | 2016-07-05 | Massachusetts Institute Of Technology | Microfluidic synthesis of organic nanoparticles |
| EP2134830A2 (en) | 2007-02-09 | 2009-12-23 | Massachusetts Institute of Technology | Oscillating cell culture bioreactor |
| WO2008124632A1 (en) | 2007-04-04 | 2008-10-16 | Massachusetts Institute Of Technology | Amphiphilic compound assisted nanoparticles for targeted delivery |
| CA2917512C (en) | 2007-10-12 | 2025-08-05 | President And Fellows Of Harvard College | VACCINE NANOTECHNOLOGY |
| US8277812B2 (en) | 2008-10-12 | 2012-10-02 | Massachusetts Institute Of Technology | Immunonanotherapeutics that provide IgG humoral response without T-cell antigen |
| US8343497B2 (en) | 2008-10-12 | 2013-01-01 | The Brigham And Women's Hospital, Inc. | Targeting of antigen presenting cells with immunonanotherapeutics |
| US8591905B2 (en) | 2008-10-12 | 2013-11-26 | The Brigham And Women's Hospital, Inc. | Nicotine immunonanotherapeutics |
| US8343498B2 (en) | 2008-10-12 | 2013-01-01 | Massachusetts Institute Of Technology | Adjuvant incorporation in immunonanotherapeutics |
| WO2010138192A2 (en) | 2009-05-27 | 2010-12-02 | Selecta Biosciences, Inc. | Nanocarriers possessing components with different rates of release |
| US20130149314A1 (en) * | 2010-02-09 | 2013-06-13 | Jörn Bullerdiek | p19Arf, HMGA2 and MDM2 For Use in the Diagnosis and Treatment of Aberrant Cell Growth |
| EP2561079A1 (en) | 2010-04-21 | 2013-02-27 | Noxxon Pharma AG | Lipid binding nucleic acids |
| CN102905728B (zh) | 2010-05-26 | 2015-11-25 | 西莱克塔生物科技公司 | 具有不偶合的佐剂的纳米载体组合物 |
| CN102375064A (zh) * | 2010-08-26 | 2012-03-14 | 杭州华得森生物技术有限公司 | 用酶联免疫法检测人体中hmga2含量的体外诊断试剂盒 |
| DE102010056610A1 (de) * | 2010-12-31 | 2012-07-05 | Volker A. Erdmann | Pharmazeutische Zusammensetzung enthaltend L-DNA |
| US20130028857A1 (en) | 2011-07-29 | 2013-01-31 | Selecta Biosciences, Inc. | Synthetic nanocarriers comprising polymers comprising multiple immunomodulatory agents |
| KR20140083039A (ko) * | 2011-10-21 | 2014-07-03 | 녹손 파르마 아게 | 글루카곤 결합형 핵산 |
| BR112014016939A2 (pt) * | 2012-01-10 | 2019-09-24 | Noxxon Pharma Ag | ácidos nucleicos que se ligam especificamente a cgrp |
| CN103566377A (zh) | 2012-07-18 | 2014-02-12 | 上海博笛生物科技有限公司 | 癌症的靶向免疫治疗 |
| US9233119B2 (en) * | 2012-09-27 | 2016-01-12 | Miami University | Use of HGMA-targeted phosphorothioate DNA aptamers to suppress carcinogenic activity and increase sensitivity to chemotherapy agents in human cancer cells |
| US10679730B2 (en) | 2013-05-28 | 2020-06-09 | The University Of Chicago | Prognostic and predictive breast cancer signature |
| WO2014193964A2 (en) * | 2013-05-28 | 2014-12-04 | Marsha Rosner | Prognostic and predictive breast cancer signature |
| EP3092255A4 (en) | 2014-01-10 | 2017-09-20 | Birdie Biopharmaceuticals Inc. | Compounds and compositions for treating egfr expressing tumors |
| CA2954446A1 (en) | 2014-07-09 | 2016-01-14 | Shanghai Birdie Biotech, Inc. | Anti-pd-l1 combinations for treating tumors |
| CN112546230A (zh) | 2014-07-09 | 2021-03-26 | 博笛生物科技有限公司 | 用于治疗癌症的联合治疗组合物和联合治疗方法 |
| KR101755617B1 (ko) | 2014-07-23 | 2017-07-10 | 한국과학기술연구원 | 삼차원 자기조립 핵산 나노입자 구조를 갖는 약물전달체 |
| CN112546238A (zh) | 2014-09-01 | 2021-03-26 | 博笛生物科技有限公司 | 用于治疗肿瘤的抗-pd-l1结合物 |
| CN107249678B (zh) * | 2014-12-19 | 2021-02-19 | 凯敏工业公司 | 使用离子电渗法进行生物活性分子的眼内递送 |
| CN107613980A (zh) | 2015-05-31 | 2018-01-19 | 源生公司 | 用于免疫疗法的组合组合物 |
| US10221249B2 (en) | 2015-09-10 | 2019-03-05 | Affigen, Llc | Method of making patient specific anti-idiotype antibodies |
| DE17829597T1 (de) * | 2016-11-30 | 2019-12-05 | Noxxon Pharma Ag | Verfahren zur polyalkoxylierung von nukleinsäuren zur rückgewinnung und wiederverwendung einer überschüssigen polyalkoxylierungsreagenz |
| US20220033813A1 (en) * | 2018-09-11 | 2022-02-03 | The Texas A&M University System | L-oligonucleotide inhibitors of polycomb repressive complex 2 (prc2) |
| KR20230005908A (ko) * | 2020-04-30 | 2023-01-10 | 벨라니디 테크놀로지스 엘엘씨 | 생물학적 또는 화학적 독립체 또는 이벤트를 검출하기 위한 분자 와이어 |
| CN112725438B (zh) * | 2021-02-05 | 2023-08-22 | 深圳市宝安区妇幼保健院 | 子宫内膜息肉甲基化标志物组合、检测试剂盒及应用 |
| CN114807183B (zh) * | 2021-12-06 | 2023-06-02 | 西藏自治区农牧科学院农业研究所 | 一种青稞矢车菊素氧甲基转移酶基因的新用途 |
| CN118311277B (zh) * | 2024-06-11 | 2024-12-31 | 北京卫未科学技术有限公司 | 一种用于癌症早筛和辅助诊断的试剂盒及其应用 |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11504926A (ja) * | 1995-05-04 | 1999-05-11 | ネクスター ファーマスーティカルズ，インコーポレイテッド | 核酸リガンド複合体 |
| JP2001504448A (ja) * | 1996-08-30 | 2001-04-03 | フュルステ，イェンス，ペーター | 核酸の鏡面対称選択および進化 |
| JP2002506441A (ja) * | 1997-06-20 | 2002-02-26 | ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング | 核酸を高等真核生物細胞に輸送するための複合体 |
| JP2004510829A (ja) * | 2000-10-09 | 2004-04-08 | バイエル アクチェンゲゼルシャフト | 核酸を細胞内に転移させるための複合体 |
| WO2004061456A2 (de) * | 2003-01-03 | 2004-07-22 | Alcedo Biotech Gmbh | Verwendungen von hmgb, hmgn, hmga proteinen |
| WO2004092191A2 (en) * | 2003-04-13 | 2004-10-28 | Enzon Pharmaceuticals, Inc. | Polymeric oligonucleotide prodrugs |
| WO2004100928A1 (de) * | 2003-05-09 | 2004-11-25 | Novosom Ag | Injizierbare liposomale depots zum wirkstoffdelivery |
| JP2005511770A (ja) * | 2001-12-19 | 2005-04-28 | アルケド バイオテック ゲーエムベーハー | Ｈｍｇｂタンパク質およびｈｍｇｂタンパク質をコードする核酸の使用方法 |
| JP2008525514A (ja) * | 2004-12-27 | 2008-07-17 | サイレンス・セラピューティクス・アーゲー | コーティングされた脂質複合体およびそれらの使用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5622828A (en) * | 1990-06-11 | 1997-04-22 | Nexstar Pharmaceuticals, Inc. | High-affinity oligonucleotide ligands to secretory phospholipase A2 (sPLA2) |
| EP1793006A3 (en) * | 1993-09-08 | 2007-08-22 | Gilead Sciences, Inc. | Nucleic acid ligands and improved methods for producing the same |
| US5780611A (en) * | 1995-09-15 | 1998-07-14 | Ramareddy Venkata Guntaka | Oligomers which inhibit expression of collagen genes |
| US6171779B1 (en) | 1996-07-12 | 2001-01-09 | University Of Medicine & Dentistry Of New Jersey | HMGI proteins in cancer |
| DE10118452A1 (de) * | 2001-04-12 | 2002-10-31 | Joern Bullerdiek | Nukleinsäuresequenzen von Hyperplasien und Tumoren der Schilddrüse |
| DK2431377T3 (en) * | 2001-10-26 | 2017-08-14 | Noxxon Pharma Ag | Modified L-nucleic acids |
| DE10211558A1 (de) | 2002-03-15 | 2003-10-09 | Noxxon Pharma Ag | Neue Formen RNAi |
| WO2003094973A1 (de) | 2002-05-08 | 2003-11-20 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Mittel und verfahren zum transport von biologisch aktiven molekülen in zellen |
| US7250496B2 (en) * | 2002-11-14 | 2007-07-31 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel regulatory genes and uses thereof |
| EP1667729A4 (en) | 2003-05-23 | 2007-10-17 | Univ Texas | STRUCTURAL BASED AND COMBINATORY SELECTED OLIGONUCLEOSIDE PHOSPHOROTHIOAT AND PHOSPHORODITHIOAT APTAMIC TARGETING AP-1 TRANSCRIPTION FACTORS |
| US7727969B2 (en) * | 2003-06-06 | 2010-06-01 | Massachusetts Institute Of Technology | Controlled release nanoparticle having bound oligonucleotide for targeted delivery |
| CN101018541A (zh) * | 2004-01-26 | 2007-08-15 | 普西维达公司 | 核酸基治疗药剂的受控和持续传输 |
-
2006
- 2006-05-04 EP EP06742799A patent/EP1877069A2/de not_active Ceased
- 2006-05-04 AU AU2006243334A patent/AU2006243334A1/en not_active Abandoned
- 2006-05-04 MX MX2007013760A patent/MX2007013760A/es unknown
- 2006-05-04 BR BRPI0610367-7A patent/BRPI0610367A2/pt not_active IP Right Cessation
- 2006-05-04 WO PCT/EP2006/004180 patent/WO2006117217A2/de not_active Ceased
- 2006-05-04 KR KR1020077025457A patent/KR101418369B1/ko not_active Expired - Fee Related
- 2006-05-04 JP JP2008509380A patent/JP2008540363A/ja active Pending
- 2006-05-04 CA CA002607185A patent/CA2607185A1/en not_active Abandoned
- 2006-05-04 US US11/913,526 patent/US8497250B2/en not_active Expired - Fee Related
- 2006-05-04 CN CN201410395203.5A patent/CN104293780A/zh active Pending
- 2006-05-04 KR KR1020147008135A patent/KR101418367B1/ko not_active Expired - Fee Related
- 2006-05-04 CN CN200680024585.4A patent/CN101217967B/zh not_active Expired - Fee Related
- 2006-05-04 SG SG201002837-1A patent/SG161311A1/en unknown
- 2006-05-04 EP EP09016125A patent/EP2206501A3/de not_active Withdrawn
-
2013
- 2013-06-20 JP JP2013129800A patent/JP5766750B2/ja not_active Expired - Fee Related
- 2013-07-30 US US13/953,797 patent/US9074214B2/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11504926A (ja) * | 1995-05-04 | 1999-05-11 | ネクスター ファーマスーティカルズ，インコーポレイテッド | 核酸リガンド複合体 |
| JP2001504448A (ja) * | 1996-08-30 | 2001-04-03 | フュルステ，イェンス，ペーター | 核酸の鏡面対称選択および進化 |
| JP2002506441A (ja) * | 1997-06-20 | 2002-02-26 | ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング | 核酸を高等真核生物細胞に輸送するための複合体 |
| JP2004510829A (ja) * | 2000-10-09 | 2004-04-08 | バイエル アクチェンゲゼルシャフト | 核酸を細胞内に転移させるための複合体 |
| JP2005511770A (ja) * | 2001-12-19 | 2005-04-28 | アルケド バイオテック ゲーエムベーハー | Ｈｍｇｂタンパク質およびｈｍｇｂタンパク質をコードする核酸の使用方法 |
| WO2004061456A2 (de) * | 2003-01-03 | 2004-07-22 | Alcedo Biotech Gmbh | Verwendungen von hmgb, hmgn, hmga proteinen |
| WO2004092191A2 (en) * | 2003-04-13 | 2004-10-28 | Enzon Pharmaceuticals, Inc. | Polymeric oligonucleotide prodrugs |
| WO2004100928A1 (de) * | 2003-05-09 | 2004-11-25 | Novosom Ag | Injizierbare liposomale depots zum wirkstoffdelivery |
| JP2008525514A (ja) * | 2004-12-27 | 2008-07-17 | サイレンス・セラピューティクス・アーゲー | コーティングされた脂質複合体およびそれらの使用 |
Non-Patent Citations (2)
| Title |
|---|
| JPN5008005774; ＭＩＳＨＲＡ Ｓ: ＥＵＲＯＰＥＡＮ ＪＯＵＲＮＡＬ ＯＦ ＣＥＬＬ ＢＩＯＬＯＧＹ Ｖ８３ Ｎ３, 2004, Ｐ９７-１１１, ＷＩＳＳＥＮＳＣＨＡＦＬＩＣＨＥ ＶＥＲＬＡＧＳＧＥＳＥＬＬＳＣＨＡＦＴ * |
| JPN5008005778; ＥＵＬＢＥＲＧ Ｄ: ＣＨＥＭＢＩＯＣＨＥＭ - Ａ ＥＵＲＯＰＥＡＮ ＪＯＵＲＮＡＬ ＯＦ ＣＨＥＭＩＣＡＬ ＢＩＯＬＯＧＹ Ｖ４ Ｎ１０, 20031006, Ｐ９７９-９８３, ＷＩＬＥＹ ＶＣＨ * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101217967A (zh) | 2008-07-09 |
| EP2206501A3 (de) | 2010-09-29 |
| US20090192100A1 (en) | 2009-07-30 |
| MX2007013760A (es) | 2008-01-28 |
| SG161311A1 (en) | 2010-05-27 |
| EP1877069A2 (de) | 2008-01-16 |
| JP2013224319A (ja) | 2013-10-31 |
| US9074214B2 (en) | 2015-07-07 |
| US20130337049A1 (en) | 2013-12-19 |
| CA2607185A1 (en) | 2006-11-09 |
| CN101217967B (zh) | 2014-09-10 |
| WO2006117217A3 (de) | 2007-08-09 |
| BRPI0610367A2 (pt) | 2010-06-15 |
| CN104293780A (zh) | 2015-01-21 |
| JP5766750B2 (ja) | 2015-08-19 |
| US8497250B2 (en) | 2013-07-30 |
| KR101418367B1 (ko) | 2014-07-25 |
| EP2206501A2 (de) | 2010-07-14 |
| KR20080009276A (ko) | 2008-01-28 |
| KR101418369B1 (ko) | 2014-07-24 |
| WO2006117217A2 (de) | 2006-11-09 |
| KR20140042941A (ko) | 2014-04-07 |
| AU2006243334A1 (en) | 2006-11-09 |
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