JP2007534308A - 複数のフルオロフォアで標識したオリゴヌクレオチド - Google Patents

複数のフルオロフォアで標識したオリゴヌクレオチド Download PDF

Info

Publication number
JP2007534308A
JP2007534308A JP2006541541A JP2006541541A JP2007534308A JP 2007534308 A JP2007534308 A JP 2007534308A JP 2006541541 A JP2006541541 A JP 2006541541A JP 2006541541 A JP2006541541 A JP 2006541541A JP 2007534308 A JP2007534308 A JP 2007534308A
Authority
JP
Japan
Prior art keywords
oligonucleotide
labeling
photometric
labeled
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP2006541541A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007534308A5 (https=
Inventor
マオ，フェイ
シン，シング
Original Assignee
アレロジック・バイオサイエンシズ・コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アレロジック・バイオサイエンシズ・コーポレーション filed Critical アレロジック・バイオサイエンシズ・コーポレーション
Publication of JP2007534308A publication Critical patent/JP2007534308A/ja
Publication of JP2007534308A5 publication Critical patent/JP2007534308A5/ja
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2006541541A 2003-11-19 2004-11-19 複数のフルオロフォアで標識したオリゴヌクレオチド Withdrawn JP2007534308A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52326303P 2003-11-19 2003-11-19
PCT/US2004/038946 WO2005051967A2 (en) 2003-11-19 2004-11-19 Oligonucleotides labeled with a plurality of fluorophores

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2011177659A Division JP5307206B2 (ja) 2003-11-19 2011-08-15 複数のフルオロフォアで標識したオリゴヌクレオチド

Publications (2)

Publication Number Publication Date
JP2007534308A true JP2007534308A (ja) 2007-11-29
JP2007534308A5 JP2007534308A5 (https=) 2011-10-06

Family

ID=34632765

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2006541541A Withdrawn JP2007534308A (ja) 2003-11-19 2004-11-19 複数のフルオロフォアで標識したオリゴヌクレオチド
JP2011177659A Expired - Fee Related JP5307206B2 (ja) 2003-11-19 2011-08-15 複数のフルオロフォアで標識したオリゴヌクレオチド

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2011177659A Expired - Fee Related JP5307206B2 (ja) 2003-11-19 2011-08-15 複数のフルオロフォアで標識したオリゴヌクレオチド

Country Status (5)

Country Link
US (2) US7667024B2 (https=)
EP (1) EP1689764B1 (https=)
JP (2) JP2007534308A (https=)
CN (1) CN101076537B (https=)
WO (1) WO2005051967A2 (https=)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013515476A (ja) * 2009-12-24 2013-05-09 シージーン アイエヌシー 偽シグナルが排除されるターゲット核酸配列のリアルタイムマルチプレッキシング検出
JP2017516482A (ja) * 2014-06-02 2017-06-22 ベース４ イノベーション リミテッド ヌクレオチド多型の検出方法
JP2020062032A (ja) * 2013-10-25 2020-04-23 バイオマジソン・インコーポレイテッドＢｉｏｍａｄｉｓｏｎ， Ｉｎｃ． 二重蛍光体を用いた酵素アッセイに使用するための細胞
JP2023103945A (ja) * 2022-01-14 2023-07-27 株式会社日立製作所 構造多型変異検出法

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8652851B2 (en) * 2004-06-17 2014-02-18 University Of Florida Research Foundation, Inc. Multi-acceptor molecular probes and applications thereof
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US9040305B2 (en) 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
WO2007100392A2 (en) 2005-11-30 2007-09-07 Biotium, Inc. Enzyme substrate comprising a functional dye and associated technology and methods
JP5308328B2 (ja) * 2006-04-04 2013-10-09 シングレックス，インコーポレイテッド トロポニンの分析のための高感度のシステムおよび方法
US7914734B2 (en) 2007-12-19 2011-03-29 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
EP2321429B1 (en) 2008-09-03 2016-08-17 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
WO2011068569A1 (en) 2009-12-04 2011-06-09 Biotium, Inc. Heterocycle-substituted xanthene dyes
CN101709335B (zh) * 2009-12-10 2011-12-21 浙江大学 一种人乳头瘤病毒常见型四重荧光定量pcr分型检测方法
JP5822843B2 (ja) * 2009-12-21 2015-11-24 シージーン アイエヌシー Ｔｓｇプライマーターゲット検出
DE102010003781B4 (de) 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
US9335289B2 (en) 2011-02-07 2016-05-10 The Governing Council Of The University Of Toronto Bioprobes and methods of use thereof
EP2705160B1 (en) * 2011-05-06 2018-08-22 Qiagen GmbH Nucleotides and oligonucleotides comprising a label associated through a linker
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
CN114717296B (zh) 2012-02-03 2025-04-04 加州理工学院 多路生化测定中信号的编码和解码
EP3901243A1 (en) 2012-08-03 2021-10-27 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
EP2943586B1 (en) 2012-12-27 2017-09-20 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
EP4570924A3 (en) * 2013-01-24 2025-09-03 California Institute of Technology Chromophore-based characterization and detection methods
HK1218096A1 (zh) 2013-03-15 2017-02-03 Nanobiosym, Inc. 用於移动设备分析核酸和蛋白质的系统和方法
EP3019559A4 (en) 2013-08-22 2017-04-05 Sony Corporation Water soluble fluorescent or colored dyes and methods for their use
WO2016081585A1 (en) * 2014-11-20 2016-05-26 Ampliwise Inc. Compositions and methods for nucleic acid amplification
KR102646956B1 (ko) 2015-02-26 2024-03-14 소니그룹주식회사 페닐에티닐나프탈렌 염료 및 이의 사용 방법
US11827661B2 (en) 2015-02-26 2023-11-28 Sony Group Corporation Water soluble fluorescent or colored dyes comprising conjugating groups
EP3400444A4 (en) 2016-01-04 2019-10-16 QuantuMDx Group Limited DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES WITH LOADING MASS MARKINGS
CN109415574B (zh) 2016-04-01 2021-05-28 索尼公司 具有刚性间隔基团的超亮二聚体或聚合物染料
AU2017240154B2 (en) 2016-04-01 2021-08-12 Sony Group Corporation Ultra bright dimeric or polymeric dyes
WO2017177065A2 (en) 2016-04-06 2017-10-12 Sony Corporation Ultra bright dimeric or polymeric dyes with spacing linker groups
EP3455299B1 (en) 2016-05-10 2024-01-17 Sony Group Corporation Compositions comprising a polymeric dye and a cyclodextrin and uses thereof
JP7527537B2 (ja) 2016-05-10 2024-08-05 ソニーグループ株式会社 ペプチド骨格を有する超明色ポリマー染料
EP3455300A1 (en) 2016-05-11 2019-03-20 Sony Corporation Ultra bright dimeric or polymeric dyes
EP3464477A1 (en) 2016-06-06 2019-04-10 Sony Corporation Ionic polymers comprising fluorescent or colored reporter groups
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
JP7312929B2 (ja) 2016-07-29 2023-07-24 ソニーグループ株式会社 超明色二量体またはポリマー色素およびその調製のための方法
KR20260011205A (ko) 2017-10-05 2026-01-22 소니그룹주식회사 프로그램가능한 중합체성 약물
WO2019071153A1 (en) 2017-10-05 2019-04-11 Sony Corporation PROGRAMMABLE DENDRITIC MEDICINES
CN111836645A (zh) 2017-11-16 2020-10-27 索尼公司 可编程的聚合药物
EP3737417B1 (en) 2018-01-12 2025-08-27 Sony Group Corporation Phosphoalkyl ribose polymers comprising biologically active compounds
US12539334B2 (en) 2018-01-12 2026-02-03 Sony Group Corporation Phosphoalkyl polymers comprising biologically active agents
CN111565756A (zh) 2018-01-12 2020-08-21 索尼公司 包含生物活性化合物的具有刚性间隔基团的聚合物
KR102742386B1 (ko) 2018-03-19 2024-12-16 소니그룹주식회사 형광 신호 향상을 위한 2가 금속의 사용
CN118480073A (zh) 2018-03-21 2024-08-13 索尼公司 具有连接体基团的聚合串联染料
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
JP7580689B2 (ja) 2018-06-27 2024-11-12 ソニーグループ株式会社 デオキシリボースを含むリンカー基を有するポリマー色素
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
KR20210032434A (ko) 2018-07-13 2021-03-24 소니 주식회사 유기인산염 단위를 포함하는 백본을 갖는 중합체성 염료
CA3105715A1 (en) 2018-07-13 2020-01-16 Quantum-Si Incorporated Biconjugatable labels and methods of use
WO2020154546A1 (en) * 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
WO2021004379A1 (zh) * 2019-07-05 2021-01-14 南京金斯瑞生物科技有限公司 一种荧光标记核酸及其合成方法
WO2021062176A2 (en) 2019-09-26 2021-04-01 Sony Corporation Polymeric tandem dyes with linker groups
EP4038081A1 (en) * 2019-09-30 2022-08-10 Sony Group Corporation Nucleotide probes
EP4256078A1 (en) 2020-12-07 2023-10-11 Sony Group Corporation Spacing linker group design for brightness enhancement in dimeric or polymeric dyes
WO2023129887A1 (en) * 2021-12-29 2023-07-06 Co-Diagnostics, Inc. Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores
US20230366038A1 (en) 2022-05-16 2023-11-16 Bioo Scientific Corporation Compositions and methods relating to loop mediated isothermal amplification (lamp)
US12347527B2 (en) * 2022-08-23 2025-07-01 Leica Microsystems Cms Gmbh Data storage device and method for storing data using an oligonucleotide nanostructure backbone and labels with dyes
WO2025207979A1 (en) * 2024-03-27 2025-10-02 3D Medicines Biomedical Technologies, Inc. Energy-transfer probes for multiplexed nucleic acid amplification assays

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5688648A (en) * 1994-02-01 1997-11-18 The Regents Of The University Of California Probes labelled with energy transfer coupled dyes

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766062A (en) 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5268486A (en) 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
WO1997027317A1 (en) * 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6312894B1 (en) * 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
AU693023B2 (en) * 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
AU713667B2 (en) * 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5696157A (en) 1996-11-15 1997-12-09 Molecular Probes, Inc. Sulfonated derivatives of 7-aminocoumarin
US6037137A (en) 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US6893868B2 (en) * 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
US5846726A (en) * 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US6130101A (en) 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US6485901B1 (en) * 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6133445A (en) 1997-12-17 2000-10-17 Carnegie Mellon University Rigidized trimethine cyanine dyes
WO1999045146A1 (en) 1998-03-05 1999-09-10 Johnson & Johnson Research Pty. Ltd. Zymogenic nucleic acid detection methods, and related molecules and kits
GB2341189B (en) 1998-08-31 2001-06-13 Amersham Pharm Biotech Inc Energy transfer dyes
US6573047B1 (en) * 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
US6472153B1 (en) 1999-10-26 2002-10-29 Epoch Biosciences, Inc. Hybridization-triggered fluorescent detection of nucleic acids
GB2359809B (en) 1999-12-24 2004-01-14 Sumitomo Chemical Co Phenylacetylene compound liquid crystal composition, polymer optically anisotropic product, and liquid crystal or optical element
WO2002014555A2 (en) * 2000-08-11 2002-02-21 University Of Utah Research Foundation Single-labeled oligonucleotide probes
CA2423806C (en) 2000-09-29 2009-12-22 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5688648A (en) * 1994-02-01 1997-11-18 The Regents Of The University Of California Probes labelled with energy transfer coupled dyes

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013515476A (ja) * 2009-12-24 2013-05-09 シージーン アイエヌシー 偽シグナルが排除されるターゲット核酸配列のリアルタイムマルチプレッキシング検出
JP2020062032A (ja) * 2013-10-25 2020-04-23 バイオマジソン・インコーポレイテッドＢｉｏｍａｄｉｓｏｎ， Ｉｎｃ． 二重蛍光体を用いた酵素アッセイに使用するための細胞
JP2017516482A (ja) * 2014-06-02 2017-06-22 ベース４ イノベーション リミテッド ヌクレオチド多型の検出方法
JP2023103945A (ja) * 2022-01-14 2023-07-27 株式会社日立製作所 構造多型変異検出法

Also Published As

Publication number Publication date
CN101076537A (zh) 2007-11-21
CN101076537B (zh) 2012-07-04
JP5307206B2 (ja) 2013-10-02
WO2005051967A2 (en) 2005-06-09
JP2011254832A (ja) 2011-12-22
US20100240103A1 (en) 2010-09-23
EP1689764B1 (en) 2013-01-02
EP1689764A2 (en) 2006-08-16
US7667024B2 (en) 2010-02-23
US20050272053A1 (en) 2005-12-08
EP1689764A4 (en) 2010-08-18
WO2005051967A3 (en) 2007-08-16

Similar Documents

Publication Publication Date Title
JP5307206B2 (ja) 複数のフルオロフォアで標識したオリゴヌクレオチド
JP3851088B2 (ja) 波長シフト性のプローブ及びプライマー
Okamoto ECHO probes: a concept of fluorescence control for practical nucleic acid sensing
US5716784A (en) Fluorescence detection assay for homogeneous PCR hybridization systems
JP4457001B2 (ja) ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法
US20230407376A1 (en) Two-part mediator probe
JP4942484B2 (ja) 改良された感度および低バックグラウンドを備えるハイブリダイゼーションによるｄｎａ検出のための蛍光プローブ
CN101831496B (zh) 多重定量核酸扩增及解链测定法
JP3045085B2 (ja) 核酸の蛍光偏光検出法
JP2006525027A (ja) 分子スイッチを含むオリゴヌクレオチド
JP2012513191A (ja) 試料中の標的核酸を検出するための方法
HUE032212T2 (en) Polymer chain reaction detection system using oligonucleotides containing phosphorothioate group
Astakhova et al. Perylene attached to 2′-amino-LNA: synthesis, incorporation into oligonucleotides, and remarkable fluorescence properties in vitro and in cell culture
EP2971147A1 (en) Multiplex allele detection
KR20040024556A (ko) 검출 시스템
US20100086916A1 (en) Oligonucleotide probes and uses thereof
ES2980651T3 (es) Método de detección de una secuencia de ácido nucleico
US20060194222A1 (en) Triplex probe compositions and methods for polynucleotide detection
JP2004049241A (ja) バックグラウンドを下げる蛍光ハイブリダイゼーションプローブ
ES2205841T3 (es) Oligonucleotidos en horquilla de transferencia de energia multifluorescente.
Whitman et al. Real-time polymerase chain reaction detection methods
Fenati et al. Single nucleotide polymorphism discrimination with and without an ethidium bromide intercalator
ES2365737T3 (es) Métodos de amplificación.
Johansson Choosing Reporter–Quencher Pairs for Efficient Quenching Through Formation of Intramolecular

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20071105

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100929

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20101228

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110111

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110131

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20110413

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20110804

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20110815

A761 Written withdrawal of application

Free format text: JAPANESE INTERMEDIATE CODE: A761

Effective date: 20110906