CN101076537B - 标记有多个荧光团的寡核苷酸 - Google Patents

标记有多个荧光团的寡核苷酸 Download PDF

Info

Publication number
CN101076537B
CN101076537B CN200480040647.1A CN200480040647A CN101076537B CN 101076537 B CN101076537 B CN 101076537B CN 200480040647 A CN200480040647 A CN 200480040647A CN 101076537 B CN101076537 B CN 101076537B
Authority
CN
China
Prior art keywords
oligonucleotide
labeled
probes
dye
dyes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN200480040647.1A
Other languages
English (en)
Chinese (zh)
Other versions
CN101076537A (zh
Inventor
毛飞
忻星
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AlleLogic Biosciences Corp
Original Assignee
AlleLogic Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AlleLogic Biosciences Corp filed Critical AlleLogic Biosciences Corp
Publication of CN101076537A publication Critical patent/CN101076537A/zh
Application granted granted Critical
Publication of CN101076537B publication Critical patent/CN101076537B/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/975Kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200480040647.1A 2003-11-19 2004-11-19 标记有多个荧光团的寡核苷酸 Expired - Fee Related CN101076537B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US52326303P 2003-11-19 2003-11-19
US60/523,263 2003-11-19
PCT/US2004/038946 WO2005051967A2 (en) 2003-11-19 2004-11-19 Oligonucleotides labeled with a plurality of fluorophores

Publications (2)

Publication Number Publication Date
CN101076537A CN101076537A (zh) 2007-11-21
CN101076537B true CN101076537B (zh) 2012-07-04

Family

ID=34632765

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200480040647.1A Expired - Fee Related CN101076537B (zh) 2003-11-19 2004-11-19 标记有多个荧光团的寡核苷酸

Country Status (5)

Country Link
US (2) US7667024B2 (https=)
EP (1) EP1689764B1 (https=)
JP (2) JP2007534308A (https=)
CN (1) CN101076537B (https=)
WO (1) WO2005051967A2 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9410196B2 (en) 2008-09-03 2016-08-09 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8652851B2 (en) * 2004-06-17 2014-02-18 University Of Florida Research Foundation, Inc. Multi-acceptor molecular probes and applications thereof
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US9040305B2 (en) 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
WO2007100392A2 (en) 2005-11-30 2007-09-07 Biotium, Inc. Enzyme substrate comprising a functional dye and associated technology and methods
JP5308328B2 (ja) * 2006-04-04 2013-10-09 シングレックス,インコーポレイテッド トロポニンの分析のための高感度のシステムおよび方法
US7914734B2 (en) 2007-12-19 2011-03-29 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
WO2011068569A1 (en) 2009-12-04 2011-06-09 Biotium, Inc. Heterocycle-substituted xanthene dyes
CN101709335B (zh) * 2009-12-10 2011-12-21 浙江大学 一种人乳头瘤病毒常见型四重荧光定量pcr分型检测方法
JP5822843B2 (ja) * 2009-12-21 2015-11-24 シージーン アイエヌシー Tsgプライマーターゲット検出
WO2011078439A1 (en) * 2009-12-24 2011-06-30 Seegene, Inc. Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals
DE102010003781B4 (de) 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
US9335289B2 (en) 2011-02-07 2016-05-10 The Governing Council Of The University Of Toronto Bioprobes and methods of use thereof
EP2705160B1 (en) * 2011-05-06 2018-08-22 Qiagen GmbH Nucleotides and oligonucleotides comprising a label associated through a linker
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
CN114717296B (zh) 2012-02-03 2025-04-04 加州理工学院 多路生化测定中信号的编码和解码
EP3901243A1 (en) 2012-08-03 2021-10-27 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
EP2943586B1 (en) 2012-12-27 2017-09-20 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
EP4570924A3 (en) * 2013-01-24 2025-09-03 California Institute of Technology Chromophore-based characterization and detection methods
HK1218096A1 (zh) 2013-03-15 2017-02-03 Nanobiosym, Inc. 用於移动设备分析核酸和蛋白质的系统和方法
EP3019559A4 (en) 2013-08-22 2017-04-05 Sony Corporation Water soluble fluorescent or colored dyes and methods for their use
WO2015061738A1 (en) * 2013-10-25 2015-04-30 Biomadison, Inc. Enzyme assay with duplicate fluorophores
WO2015185564A1 (en) 2014-06-02 2015-12-10 Base4 Innovation Ltd Nucleotide polymorphism detection method
WO2016081585A1 (en) * 2014-11-20 2016-05-26 Ampliwise Inc. Compositions and methods for nucleic acid amplification
KR102646956B1 (ko) 2015-02-26 2024-03-14 소니그룹주식회사 페닐에티닐나프탈렌 염료 및 이의 사용 방법
US11827661B2 (en) 2015-02-26 2023-11-28 Sony Group Corporation Water soluble fluorescent or colored dyes comprising conjugating groups
EP3400444A4 (en) 2016-01-04 2019-10-16 QuantuMDx Group Limited DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES WITH LOADING MASS MARKINGS
CN109415574B (zh) 2016-04-01 2021-05-28 索尼公司 具有刚性间隔基团的超亮二聚体或聚合物染料
AU2017240154B2 (en) 2016-04-01 2021-08-12 Sony Group Corporation Ultra bright dimeric or polymeric dyes
WO2017177065A2 (en) 2016-04-06 2017-10-12 Sony Corporation Ultra bright dimeric or polymeric dyes with spacing linker groups
EP3455299B1 (en) 2016-05-10 2024-01-17 Sony Group Corporation Compositions comprising a polymeric dye and a cyclodextrin and uses thereof
JP7527537B2 (ja) 2016-05-10 2024-08-05 ソニーグループ株式会社 ペプチド骨格を有する超明色ポリマー染料
EP3455300A1 (en) 2016-05-11 2019-03-20 Sony Corporation Ultra bright dimeric or polymeric dyes
EP3464477A1 (en) 2016-06-06 2019-04-10 Sony Corporation Ionic polymers comprising fluorescent or colored reporter groups
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
JP7312929B2 (ja) 2016-07-29 2023-07-24 ソニーグループ株式会社 超明色二量体またはポリマー色素およびその調製のための方法
KR20260011205A (ko) 2017-10-05 2026-01-22 소니그룹주식회사 프로그램가능한 중합체성 약물
WO2019071153A1 (en) 2017-10-05 2019-04-11 Sony Corporation PROGRAMMABLE DENDRITIC MEDICINES
CN111836645A (zh) 2017-11-16 2020-10-27 索尼公司 可编程的聚合药物
EP3737417B1 (en) 2018-01-12 2025-08-27 Sony Group Corporation Phosphoalkyl ribose polymers comprising biologically active compounds
US12539334B2 (en) 2018-01-12 2026-02-03 Sony Group Corporation Phosphoalkyl polymers comprising biologically active agents
CN111565756A (zh) 2018-01-12 2020-08-21 索尼公司 包含生物活性化合物的具有刚性间隔基团的聚合物
KR102742386B1 (ko) 2018-03-19 2024-12-16 소니그룹주식회사 형광 신호 향상을 위한 2가 금속의 사용
CN118480073A (zh) 2018-03-21 2024-08-13 索尼公司 具有连接体基团的聚合串联染料
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
JP7580689B2 (ja) 2018-06-27 2024-11-12 ソニーグループ株式会社 デオキシリボースを含むリンカー基を有するポリマー色素
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
KR20210032434A (ko) 2018-07-13 2021-03-24 소니 주식회사 유기인산염 단위를 포함하는 백본을 갖는 중합체성 염료
CA3105715A1 (en) 2018-07-13 2020-01-16 Quantum-Si Incorporated Biconjugatable labels and methods of use
WO2020154546A1 (en) * 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
WO2021004379A1 (zh) * 2019-07-05 2021-01-14 南京金斯瑞生物科技有限公司 一种荧光标记核酸及其合成方法
WO2021062176A2 (en) 2019-09-26 2021-04-01 Sony Corporation Polymeric tandem dyes with linker groups
EP4038081A1 (en) * 2019-09-30 2022-08-10 Sony Group Corporation Nucleotide probes
EP4256078A1 (en) 2020-12-07 2023-10-11 Sony Group Corporation Spacing linker group design for brightness enhancement in dimeric or polymeric dyes
WO2023129887A1 (en) * 2021-12-29 2023-07-06 Co-Diagnostics, Inc. Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores
JP2023103945A (ja) * 2022-01-14 2023-07-27 株式会社日立製作所 構造多型変異検出法
US20230366038A1 (en) 2022-05-16 2023-11-16 Bioo Scientific Corporation Compositions and methods relating to loop mediated isothermal amplification (lamp)
US12347527B2 (en) * 2022-08-23 2025-07-01 Leica Microsystems Cms Gmbh Data storage device and method for storing data using an oligonucleotide nanostructure backbone and labels with dyes
WO2025207979A1 (en) * 2024-03-27 2025-10-02 3D Medicines Biomedical Technologies, Inc. Energy-transfer probes for multiplexed nucleic acid amplification assays

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US6150097A (en) * 1996-04-12 2000-11-21 The Public Health Research Institute Of The City Of New York, Inc. Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes
US6573047B1 (en) * 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766062A (en) 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5268486A (en) 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
WO1997027317A1 (en) * 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6312894B1 (en) * 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
AU693023B2 (en) * 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Pcr中のハイブリダイゼーションのモニタリング
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5696157A (en) 1996-11-15 1997-12-09 Molecular Probes, Inc. Sulfonated derivatives of 7-aminocoumarin
US6037137A (en) 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US6893868B2 (en) * 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
US5846726A (en) * 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US6130101A (en) 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US6485901B1 (en) * 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6133445A (en) 1997-12-17 2000-10-17 Carnegie Mellon University Rigidized trimethine cyanine dyes
WO1999045146A1 (en) 1998-03-05 1999-09-10 Johnson & Johnson Research Pty. Ltd. Zymogenic nucleic acid detection methods, and related molecules and kits
GB2341189B (en) 1998-08-31 2001-06-13 Amersham Pharm Biotech Inc Energy transfer dyes
US6472153B1 (en) 1999-10-26 2002-10-29 Epoch Biosciences, Inc. Hybridization-triggered fluorescent detection of nucleic acids
GB2359809B (en) 1999-12-24 2004-01-14 Sumitomo Chemical Co Phenylacetylene compound liquid crystal composition, polymer optically anisotropic product, and liquid crystal or optical element
WO2002014555A2 (en) * 2000-08-11 2002-02-21 University Of Utah Research Foundation Single-labeled oligonucleotide probes
CA2423806C (en) 2000-09-29 2009-12-22 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6150097A (en) * 1996-04-12 2000-11-21 The Public Health Research Institute Of The City Of New York, Inc. Nucleic acid detection probes having non-FRET fluorescence quenching and kits and assays including such probes
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US6573047B1 (en) * 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9410196B2 (en) 2008-09-03 2016-08-09 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US9938573B2 (en) 2008-09-03 2018-04-10 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags

Also Published As

Publication number Publication date
CN101076537A (zh) 2007-11-21
JP5307206B2 (ja) 2013-10-02
WO2005051967A2 (en) 2005-06-09
JP2011254832A (ja) 2011-12-22
US20100240103A1 (en) 2010-09-23
EP1689764B1 (en) 2013-01-02
EP1689764A2 (en) 2006-08-16
US7667024B2 (en) 2010-02-23
JP2007534308A (ja) 2007-11-29
US20050272053A1 (en) 2005-12-08
EP1689764A4 (en) 2010-08-18
WO2005051967A3 (en) 2007-08-16

Similar Documents

Publication Publication Date Title
CN101076537B (zh) 标记有多个荧光团的寡核苷酸
JP4942484B2 (ja) 改良された感度および低バックグラウンドを備えるハイブリダイゼーションによるdna検出のための蛍光プローブ
US20230407376A1 (en) Two-part mediator probe
JP4040087B2 (ja) Pcr増幅とハイブリダイゼーションプロービングアッセイとを組み合わせた方法およびそのための試薬
US6902900B2 (en) Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
JP4131749B2 (ja) 自己消光性蛍光プローブと方法
JP2002521069A (ja) 波長シフト性のプローブ及びプライマー
JP2004509613A (ja) 非同調的刺激pcr
US6803201B2 (en) Compositions and methods for polynucleotide sequence determination
Astakhova et al. Perylene attached to 2′-amino-LNA: synthesis, incorporation into oligonucleotides, and remarkable fluorescence properties in vitro and in cell culture
CN101285094A (zh) 引物、成套引物、使用其的核酸扩增方法和变异检测方法
US8354523B2 (en) Oligonucleotide probes and uses thereof
JP3970816B2 (ja) バックグラウンドを下げる蛍光ハイブリダイゼーションプローブ
US20060194222A1 (en) Triplex probe compositions and methods for polynucleotide detection
Bai et al. A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes
EP1585832B1 (en) Compositions and methods for polynucleotide detection
ES2365737T3 (es) Métodos de amplificación.

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120704

Termination date: 20131119