JP2007105265A - 生物由来スキャフォールドの作製方法 - Google Patents
生物由来スキャフォールドの作製方法 Download PDFInfo
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- JP2007105265A JP2007105265A JP2005299590A JP2005299590A JP2007105265A JP 2007105265 A JP2007105265 A JP 2007105265A JP 2005299590 A JP2005299590 A JP 2005299590A JP 2005299590 A JP2005299590 A JP 2005299590A JP 2007105265 A JP2007105265 A JP 2007105265A
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- glutaraldehyde
- elastase
- elastin
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 7
- 210000001519 tissue Anatomy 0.000 claims abstract description 32
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims abstract description 27
- 102000016387 Pancreatic elastase Human genes 0.000 claims abstract description 17
- 108010067372 Pancreatic elastase Proteins 0.000 claims abstract description 17
- 238000002054 transplantation Methods 0.000 claims abstract description 8
- 210000004872 soft tissue Anatomy 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 9
- 238000004132 cross linking Methods 0.000 claims description 8
- 239000007983 Tris buffer Substances 0.000 claims description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical group OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 230000002308 calcification Effects 0.000 abstract description 5
- 238000001338 self-assembly Methods 0.000 abstract 1
- 102000016942 Elastin Human genes 0.000 description 21
- 108010014258 Elastin Proteins 0.000 description 21
- 229920002549 elastin Polymers 0.000 description 21
- 210000004204 blood vessel Anatomy 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- 210000000709 aorta Anatomy 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 230000007515 enzymatic degradation Effects 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 230000008595 infiltration Effects 0.000 description 4
- 238000001764 infiltration Methods 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 101710172711 Structural protein Proteins 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 238000000879 optical micrograph Methods 0.000 description 3
- 230000001172 regenerating effect Effects 0.000 description 3
- 238000007447 staining method Methods 0.000 description 3
- 239000003146 anticoagulant agent Substances 0.000 description 2
- -1 aromatic amino acids Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000003709 heart valve Anatomy 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000031481 Pathologic Constriction Diseases 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 229960004676 antithrombotic agent Drugs 0.000 description 1
- 210000001765 aortic valve Anatomy 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 239000002473 artificial blood Substances 0.000 description 1
- 229920002988 biodegradable polymer Polymers 0.000 description 1
- 239000004621 biodegradable polymer Substances 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 150000004985 diamines Chemical class 0.000 description 1
- 230000007159 enucleation Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000001631 haemodialysis Methods 0.000 description 1
- 230000000322 hemodialysis Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 239000004626 polylactic acid Substances 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000036262 stenosis Effects 0.000 description 1
- 208000037804 stenosis Diseases 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Dispersion Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Materials For Medical Uses (AREA)
Abstract
【解決手段】(a)生体軟組織をグルタルアルデヒドで部分的に架橋固定するステップ、および(b)部分的に架橋固定化した組織をエラスターゼと共にインキュベートするステップ、
を含む、移植用スキャフォールドの作製方法。
【選択図】なし
Description
(a)生態軟組織をグルタルアルデヒドで部分的に架橋固定化するステップ、および
(b)部分的に架橋固定化した組織をエラスターゼと共にインキュベートし、選択的にエラスチンを除去するステップを含む、本発明の移植用スキャフォールドの作製方法によって達成される。
と反応しなくなるまでグルタルアルデヒド水溶液中に浸漬して行われる。このようにグルタルアルデヒドと完全に反応させた組織内の構造タンパクは不溶性に変性し、生分解性でもなくタンパク分解酵素の基質にもならない。しかしながら構造タンパクに含まれるグルタルアルデヒドと反応し得る官能基の多くを未反応のまま残して反応を停止することにより、組織を部分的に架橋することができる。
GA未処理、0.01%GA,0.1%GA,1%GAおよび25%GAに常温でそれぞれ1時間浸漬し、上の一定条件でエラスチンを分解処理した。図1の下列は輪切したブタ大動脈の外観を示し、上列はヘマトキシリン−エオジン染色した後の組織の拡大光学顕微鏡写真である。見られるように、GA未処理およびGA0.01%処理のものはエラスチンの酵素分解除去により組織が過度に弛緩し、原形を保っていないが、1%および25%GA処理したものはGAがエラスチンにも作用し、酵素分解後も組織内にエラスチンが残留している様子が観察された。0.1%GAでは組織の原形が保たれたまま、エラスチンがほぼ完全に除去され、ドナー由来細胞の脱核も観察される。
実施例1と同様に、GA無処理,0.1%GA,1%GAおよび10%GA処理後エラスターゼ処理したブタ大動脈のヘマトキシリン−エオジン染色した組織を異なる倍率で光学顕微鏡写真撮影した。結果を図2に示す。見られるようにGA未処理のものは過度の弛緩が認められ、GA1%および10%の処理では暗色のヒモ状部分として見えるエラスチンおよびドナー由来細胞核の残留が多く認められる。
実施例2と同じ条件で処理したブタ大動脈組織をエラスチカ・ワンギーソン染色法により染色し、拡大光学顕微鏡写真撮影した。結果を図3に示す。この染色法はエラスチン線維を黒紫色に、コラーゼン線維を赤色に染め分ける染色法である。モノクロム写真ではエラスチン線維がより濃暗色に写るので、見られるようにGA濃度が高くなるにつれ、エラスチン線維が多く残留することが見られる。
Claims (6)
- (a)生体軟組織をグルタルアルデヒドで部分的に架橋固定するステップ、および
(b)部分的に架橋固定化した組織をエラスターゼと共にインキュベートするステップ、
を含む、移植用スキャフォールドの作製方法。 - ステップ(a)は、組織を0.1%またはそれ未満のグルタルアルデヒド水溶液中に5時間以下の期間浸漬することによって実施される請求項1の方法。
- グルタルアルデヒド濃度が0.1%であり、浸漬時間が常温において1時間である請求項2の方法。
- ステップ(b)は、緩衝液中エラスターゼの至適pHにおいて行われる請求項1ないし3のいずれかの方法。
- 緩衝液が7.5ないし8.5のpHを有するトリス緩衝液である請求項4の方法。
- ステップ(b)の後、組織を洗浄するステップをさらに含んでいる請求項1ないし5のいずれかの方法。
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005299590A JP5002805B2 (ja) | 2005-10-14 | 2005-10-14 | 生物由来スキャフォールドの作製方法 |
PCT/JP2006/320181 WO2007043513A1 (ja) | 2005-10-14 | 2006-10-10 | 生物由来スキャフォールドの作製方法 |
EP06811494A EP1935439A4 (en) | 2005-10-14 | 2006-10-10 | PROCESS FOR PRODUCING A BIO-DERIVED FRAME |
US12/090,197 US8415125B2 (en) | 2005-10-14 | 2006-10-10 | Method for preparing biological scaffold material |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005299590A JP5002805B2 (ja) | 2005-10-14 | 2005-10-14 | 生物由来スキャフォールドの作製方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007105265A true JP2007105265A (ja) | 2007-04-26 |
JP5002805B2 JP5002805B2 (ja) | 2012-08-15 |
Family
ID=37942749
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2005299590A Active JP5002805B2 (ja) | 2005-10-14 | 2005-10-14 | 生物由来スキャフォールドの作製方法 |
Country Status (4)
Country | Link |
---|---|
US (1) | US8415125B2 (ja) |
EP (1) | EP1935439A4 (ja) |
JP (1) | JP5002805B2 (ja) |
WO (1) | WO2007043513A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008029653A (ja) * | 2006-07-31 | 2008-02-14 | Japan Health Science Foundation | 生物由来スキャフォールドの作製方法 |
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US9259511B2 (en) * | 2008-06-06 | 2016-02-16 | Lifecell Corporation | Elastase treatment of tissue matrices |
US8469779B1 (en) | 2009-01-02 | 2013-06-25 | Lifecell Corporation | Method for debristling animal skin |
US8637067B1 (en) | 2011-03-10 | 2014-01-28 | Lifecell Corporation | Elastic tissue matrix derived hydrogel |
EP2696908B1 (en) | 2011-04-14 | 2015-03-11 | Lifecell Corporation | Regenerative materials |
KR102068071B1 (ko) | 2011-04-28 | 2020-01-20 | 라이프셀 코포레이션 | 조직 생성물의 효소처리방법 |
US10207025B2 (en) | 2011-04-28 | 2019-02-19 | Lifecell Corporation | Method for enzymatic treatment of tissue products |
US9238793B2 (en) | 2011-04-28 | 2016-01-19 | Lifecell Corporation | Method for enzymatic treatment of tissue products |
US9089523B2 (en) | 2011-07-28 | 2015-07-28 | Lifecell Corporation | Natural tissue scaffolds as tissue fillers |
BR112014014975B1 (pt) | 2011-12-20 | 2019-06-25 | Lifecell Corporation | Produto de tecido, e método para produzir uma composição de tecido |
ES2864104T3 (es) | 2011-12-20 | 2021-10-13 | Lifecell Corp | Productos tisulares laminados |
AU2013212592B2 (en) | 2012-01-24 | 2016-06-30 | Lifecell Corporation | Elongated tissue matrices |
ES2716993T3 (es) | 2012-03-08 | 2019-06-18 | Lifecell Corp | Matrices de colágeno y tejido activadas por enzimas |
EP2841116A1 (en) | 2012-04-24 | 2015-03-04 | Lifecell Corporation | Flowable tissue matrices |
BR112014026088B1 (pt) | 2012-04-24 | 2019-11-05 | Lifecell Corp | produto de tratamento de tecidos |
BR112015000547B1 (pt) | 2012-07-13 | 2020-11-10 | Lifecell Corporation | método para tratar tecido |
AU2013323747B2 (en) | 2012-09-26 | 2017-02-02 | Lifecell Corporation | Processed adipose tissue |
EP3659633A1 (en) | 2013-02-06 | 2020-06-03 | LifeCell Corporation | Methods for localized modification of tissue products |
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US10792394B2 (en) | 2016-06-03 | 2020-10-06 | Lifecell Corporation | Methods for localized modification of tissue products |
EP3558405A1 (en) | 2016-12-22 | 2019-10-30 | LifeCell Corporation | Devices and methods for tissue cryomilling |
WO2018140855A1 (en) | 2017-01-30 | 2018-08-02 | Lifecell Corporation | Devices including muscle matrix and methods of production and use |
JP6942191B2 (ja) | 2017-01-30 | 2021-09-29 | ライフセル コーポレーションLifeCell Corporation | トランスグルタミナーゼ処理製品 |
US11123375B2 (en) | 2017-10-18 | 2021-09-21 | Lifecell Corporation | Methods of treating tissue voids following removal of implantable infusion ports using adipose tissue products |
CN111201046B (zh) | 2017-10-18 | 2022-06-28 | 生命细胞公司 | 脂肪组织产品以及产生方法 |
ES2960617T3 (es) | 2017-10-19 | 2024-03-05 | Lifecell Corp | Productos de matriz de tejido acelular fluida y métodos de producción |
US11246994B2 (en) | 2017-10-19 | 2022-02-15 | Lifecell Corporation | Methods for introduction of flowable acellular tissue matrix products into a hand |
US11633521B2 (en) | 2019-05-30 | 2023-04-25 | Lifecell Corporation | Biologic breast implant |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000516489A (ja) * | 1996-07-30 | 2000-12-12 | バクスター インターナショナル インコーポレイテッド | グルタルアルデヒド固定化した生体補綴物におけるカルシウム沈着を軽減しそして耐久性を改善するための方法、およびこの方法によって製造される人工物 |
WO2002096978A1 (fr) * | 2001-05-30 | 2002-12-05 | Keiichi Miyamoto | Elastine reticulee et son procede de production |
WO2004087232A1 (ja) * | 2003-03-31 | 2004-10-14 | Teijin Limited | エラスチン成形体およびその製造法 |
Family Cites Families (3)
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JPS5230097A (en) | 1975-09-02 | 1977-03-07 | Kaneyasu Miyata | Method of mounting different substitute blood vessel |
US4399123A (en) * | 1980-04-01 | 1983-08-16 | Oliver Roy F | Fibrous tissue dressing or implant |
US4378224A (en) * | 1980-09-19 | 1983-03-29 | Nimni Marcel E | Coating for bioprosthetic device and method of making same |
-
2005
- 2005-10-14 JP JP2005299590A patent/JP5002805B2/ja active Active
-
2006
- 2006-10-10 US US12/090,197 patent/US8415125B2/en not_active Expired - Fee Related
- 2006-10-10 WO PCT/JP2006/320181 patent/WO2007043513A1/ja active Application Filing
- 2006-10-10 EP EP06811494A patent/EP1935439A4/en not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2000516489A (ja) * | 1996-07-30 | 2000-12-12 | バクスター インターナショナル インコーポレイテッド | グルタルアルデヒド固定化した生体補綴物におけるカルシウム沈着を軽減しそして耐久性を改善するための方法、およびこの方法によって製造される人工物 |
WO2002096978A1 (fr) * | 2001-05-30 | 2002-12-05 | Keiichi Miyamoto | Elastine reticulee et son procede de production |
WO2004087232A1 (ja) * | 2003-03-31 | 2004-10-14 | Teijin Limited | エラスチン成形体およびその製造法 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008029653A (ja) * | 2006-07-31 | 2008-02-14 | Japan Health Science Foundation | 生物由来スキャフォールドの作製方法 |
Also Published As
Publication number | Publication date |
---|---|
WO2007043513A1 (ja) | 2007-04-19 |
EP1935439A1 (en) | 2008-06-25 |
US8415125B2 (en) | 2013-04-09 |
JP5002805B2 (ja) | 2012-08-15 |
EP1935439A4 (en) | 2012-03-14 |
US20100021961A1 (en) | 2010-01-28 |
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