JP5002805B2 - 生物由来スキャフォールドの作製方法 - Google Patents
生物由来スキャフォールドの作製方法 Download PDFInfo
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- JP5002805B2 JP5002805B2 JP2005299590A JP2005299590A JP5002805B2 JP 5002805 B2 JP5002805 B2 JP 5002805B2 JP 2005299590 A JP2005299590 A JP 2005299590A JP 2005299590 A JP2005299590 A JP 2005299590A JP 5002805 B2 JP5002805 B2 JP 5002805B2
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- tissue
- glutaraldehyde
- elastin
- elastase
- treated
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
Description
(a)生態軟組織をグルタルアルデヒドで部分的に架橋固定化するステップ、および
(b)部分的に架橋固定化した組織をエラスターゼと共にインキュベートし、選択的にエラスチンを除去するステップを含む、本発明の移植用スキャフォールドの作製方法によって達成される。
と反応しなくなるまでグルタルアルデヒド水溶液中に浸漬して行われる。このようにグルタルアルデヒドと完全に反応させた組織内の構造タンパクは不溶性に変性し、生分解性でもなくタンパク分解酵素の基質にもならない。しかしながら構造タンパクに含まれるグルタルアルデヒドと反応し得る官能基の多くを未反応のまま残して反応を停止することにより、組織を部分的に架橋することができる。
GA未処理、0.01%GA,0.1%GA,1%GAおよび25%GAに常温でそれぞれ1時間浸漬し、上の一定条件でエラスチンを分解処理した。図1の下列は輪切したブタ大動脈の外観を示し、上列はヘマトキシリン−エオジン染色した後の組織の拡大光学顕微鏡写真である。見られるように、GA未処理およびGA0.01%処理のものはエラスチンの酵素分解除去により組織が過度に弛緩し、原形を保っていないが、1%および25%GA処理したものはGAがエラスチンにも作用し、酵素分解後も組織内にエラスチンが残留している様子が観察された。0.1%GAでは組織の原形が保たれたまま、エラスチンがほぼ完全に除去され、ドナー由来細胞の脱核も観察される。
実施例1と同様に、GA無処理,0.1%GA,1%GAおよび10%GA処理後エラスターゼ処理したブタ大動脈のヘマトキシリン−エオジン染色した組織を異なる倍率で光学顕微鏡写真撮影した。結果を図2に示す。見られるようにGA未処理のものは過度の弛緩が認められ、GA1%および10%の処理では暗色のヒモ状部分として見えるエラスチンおよびドナー由来細胞核の残留が多く認められる。
実施例2と同じ条件で処理したブタ大動脈組織をエラスチカ・ワンギーソン染色法により染色し、拡大光学顕微鏡写真撮影した。結果を図3に示す。この染色法はエラスチン線維を黒紫色に、コラーゼン線維を赤色に染め分ける染色法である。モノクロム写真ではエラスチン線維がより濃暗色に写るので、見られるようにGA濃度が高くなるにつれ、エラスチン線維が多く残留することが見られる。
Claims (6)
- (a)生体軟組織をグルタルアルデヒドで部分的に架橋固定するステップ、および
(b)部分的に架橋固定化した組織をエラスターゼと共にインキュベートするステップ、
を含む、移植用スキャフォールドの作製方法。 - ステップ(a)は、組織を0.1%またはそれ未満のグルタルアルデヒド水溶液中に5時間以下の期間浸漬することによって実施される請求項1の方法。
- グルタルアルデヒド濃度が0.1%であり、浸漬時間が常温において1時間である請求項2の方法。
- ステップ(b)は、緩衝液中エラスターゼの至適pHにおいて行われる請求項1ないし3のいずれかの方法。
- 緩衝液が7.5ないし8.5のpHを有するトリス緩衝液である請求項4の方法。
- ステップ(b)の後、組織を洗浄するステップをさらに含んでいる請求項1ないし5のいずれかの方法。
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005299590A JP5002805B2 (ja) | 2005-10-14 | 2005-10-14 | 生物由来スキャフォールドの作製方法 |
PCT/JP2006/320181 WO2007043513A1 (ja) | 2005-10-14 | 2006-10-10 | 生物由来スキャフォールドの作製方法 |
EP06811494A EP1935439A4 (en) | 2005-10-14 | 2006-10-10 | PROCESS FOR PRODUCING A BIO-DERIVED FRAME |
US12/090,197 US8415125B2 (en) | 2005-10-14 | 2006-10-10 | Method for preparing biological scaffold material |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005299590A JP5002805B2 (ja) | 2005-10-14 | 2005-10-14 | 生物由来スキャフォールドの作製方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007105265A JP2007105265A (ja) | 2007-04-26 |
JP5002805B2 true JP5002805B2 (ja) | 2012-08-15 |
Family
ID=37942749
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2005299590A Active JP5002805B2 (ja) | 2005-10-14 | 2005-10-14 | 生物由来スキャフォールドの作製方法 |
Country Status (4)
Country | Link |
---|---|
US (1) | US8415125B2 (ja) |
EP (1) | EP1935439A4 (ja) |
JP (1) | JP5002805B2 (ja) |
WO (1) | WO2007043513A1 (ja) |
Families Citing this family (28)
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---|---|---|---|---|
JP5050197B2 (ja) * | 2006-07-31 | 2012-10-17 | 財団法人ヒューマンサイエンス振興財団 | 生物由来スキャフォールドの作製方法 |
EP2280740B1 (en) | 2008-06-06 | 2015-04-01 | Lifecell Corporation | Elastase treatment of tissue matrices |
US8469779B1 (en) | 2009-01-02 | 2013-06-25 | Lifecell Corporation | Method for debristling animal skin |
US8637067B1 (en) | 2011-03-10 | 2014-01-28 | Lifecell Corporation | Elastic tissue matrix derived hydrogel |
EP2696908B1 (en) | 2011-04-14 | 2015-03-11 | Lifecell Corporation | Regenerative materials |
US10207025B2 (en) | 2011-04-28 | 2019-02-19 | Lifecell Corporation | Method for enzymatic treatment of tissue products |
SG10201802843WA (en) | 2011-04-28 | 2018-05-30 | Lifecell Corp | Method for enzymatic treatment of tissue products |
US9238793B2 (en) | 2011-04-28 | 2016-01-19 | Lifecell Corporation | Method for enzymatic treatment of tissue products |
US9089523B2 (en) | 2011-07-28 | 2015-07-28 | Lifecell Corporation | Natural tissue scaffolds as tissue fillers |
DK3501558T3 (da) | 2011-12-20 | 2021-03-01 | Lifecell Corp | Flydbare vævsprodukter |
EP2793964B1 (en) | 2011-12-20 | 2019-03-20 | LifeCell Corporation | Sheet tissue products |
US9271821B2 (en) | 2012-01-24 | 2016-03-01 | Lifecell Corporation | Elongated tissue matrices |
WO2013134009A1 (en) | 2012-03-08 | 2013-09-12 | Lifecell Corporation | Enzyme-activated collagen and tissue matrices |
WO2013162997A1 (en) | 2012-04-24 | 2013-10-31 | Lifecell Corporation | Functionalized tissue matrices |
BR112014026090A8 (pt) | 2012-04-24 | 2019-08-27 | Lifecell Corp | composição de matriz tecidual apta a fluir |
WO2014011402A1 (en) | 2012-07-13 | 2014-01-16 | Lifecell Corporation | Methods for improved treatment of adipose tissue |
AU2013323747B2 (en) | 2012-09-26 | 2017-02-02 | Lifecell Corporation | Processed adipose tissue |
EP3659633A1 (en) | 2013-02-06 | 2020-06-03 | LifeCell Corporation | Methods for localized modification of tissue products |
WO2015066668A1 (en) | 2013-11-04 | 2015-05-07 | Lifecell Corporation | Methods of removing alpha-galactose |
WO2017210109A1 (en) | 2016-06-03 | 2017-12-07 | Lifecell Corporation | Methods for localized modification of tissue products |
US11045583B2 (en) | 2016-12-22 | 2021-06-29 | Lifecell Corporation | Devices and methods for tissue cryomilling |
US10413634B2 (en) | 2017-01-30 | 2019-09-17 | Lifecell Corporation | Transglutaminase treated products |
WO2018140855A1 (en) | 2017-01-30 | 2018-08-02 | Lifecell Corporation | Devices including muscle matrix and methods of production and use |
US10821205B2 (en) | 2017-10-18 | 2020-11-03 | Lifecell Corporation | Adipose tissue products and methods of production |
US11123375B2 (en) | 2017-10-18 | 2021-09-21 | Lifecell Corporation | Methods of treating tissue voids following removal of implantable infusion ports using adipose tissue products |
US11246994B2 (en) | 2017-10-19 | 2022-02-15 | Lifecell Corporation | Methods for introduction of flowable acellular tissue matrix products into a hand |
EP3697462B1 (en) | 2017-10-19 | 2023-07-12 | LifeCell Corporation | Flowable acellular tissue matrix products and methods of production |
WO2020243497A1 (en) | 2019-05-30 | 2020-12-03 | Lifecell Corporation | Biologic breast implant |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5230097A (en) | 1975-09-02 | 1977-03-07 | Kaneyasu Miyata | Method of mounting different substitute blood vessel |
US4399123A (en) * | 1980-04-01 | 1983-08-16 | Oliver Roy F | Fibrous tissue dressing or implant |
US4378224A (en) | 1980-09-19 | 1983-03-29 | Nimni Marcel E | Coating for bioprosthetic device and method of making same |
US5782931A (en) * | 1996-07-30 | 1998-07-21 | Baxter International Inc. | Methods for mitigating calcification and improving durability in glutaraldehyde-fixed bioprostheses and articles manufactured by such methods |
JP4214051B2 (ja) * | 2001-05-30 | 2009-01-28 | 啓一 宮本 | エラスチン架橋体およびその製造方法 |
CN100358588C (zh) * | 2003-03-31 | 2008-01-02 | 帝人株式会社 | 弹性蛋白成型体及其制备方法 |
-
2005
- 2005-10-14 JP JP2005299590A patent/JP5002805B2/ja active Active
-
2006
- 2006-10-10 US US12/090,197 patent/US8415125B2/en not_active Expired - Fee Related
- 2006-10-10 EP EP06811494A patent/EP1935439A4/en not_active Withdrawn
- 2006-10-10 WO PCT/JP2006/320181 patent/WO2007043513A1/ja active Application Filing
Also Published As
Publication number | Publication date |
---|---|
EP1935439A4 (en) | 2012-03-14 |
WO2007043513A1 (ja) | 2007-04-19 |
US8415125B2 (en) | 2013-04-09 |
JP2007105265A (ja) | 2007-04-26 |
EP1935439A1 (en) | 2008-06-25 |
US20100021961A1 (en) | 2010-01-28 |
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