JP5050197B2 - 生物由来スキャフォールドの作製方法 - Google Patents
生物由来スキャフォールドの作製方法 Download PDFInfo
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- JP5050197B2 JP5050197B2 JP2006207384A JP2006207384A JP5050197B2 JP 5050197 B2 JP5050197 B2 JP 5050197B2 JP 2006207384 A JP2006207384 A JP 2006207384A JP 2006207384 A JP2006207384 A JP 2006207384A JP 5050197 B2 JP5050197 B2 JP 5050197B2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
- A61L27/3645—Connective tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
Description
(a)生体軟組織を凍結乾燥後、含まれるタンパク質を部分的に架橋固定化するため真空下100℃以上200℃以下の温度において加熱するステップ、
(b)部分的に架橋固定化した組織をエラスターゼと共にインキュベートし、選択的にエラスチンを除去するステップを含む移植用スキャフォールドの作製方法を提供する。
(a)生体軟組織を媒体中超高静水圧を印加することによって脱細胞するステップ、
(b)脱細胞した生体軟組織を凍結乾燥後、含まれるタンパク質を部分的に架橋固定化するため100℃以上200℃以下の温度で加熱するステップ、
(c)部分的に架橋固定化した組織をエラスターゼと共にインキュベートし、選択的にエラスチンを除去するステップを含む移植用スキャフォールドの作成方法を提供する。
ブタ大動脈血管を直接凍結乾燥し、これをそれぞれ100℃、120℃、160℃および180℃で真空オーブン中24時間加熱し、凍結乾燥後の絶乾重量と真空熱架橋後の重量との差から重量損失率(%)を算出した。結果を図1のグラフに示す。
実施例で用いたものと同様なブタ大動脈血管を無菌水と共に超高静水圧印加装置(神戸製鋼所製ドクターシェフ)へ入れ、圧力10000気圧で10分間超高静水圧を印加し、取出したブタ大動脈血管を無菌水で充分に洗浄し、脱細胞処理した。
Claims (13)
- (a)生体軟組織を凍結乾燥後、含まれるタンパク質を部分的に架橋固定化するため真空下100℃以上200℃以下の温度において加熱するステップ、
(b)部分的に架橋固定化した組織をエラスターゼと共にインキュベートし、選択的にエラスチンを除去するステップ、
を含む移植用スキャフォールドの作成方法。 - 前記軟組織は主としてコラーゲンとエラスチンよりなる結合組織である請求項1の方法。
- ステップ(a)の凍結乾燥後の軟組織の加熱は、少なくとも温度120℃において24時間行われる請求項1の方法。
- ステップ(b)は、エラスターゼの至適pHを有する緩衝液中で実施される請求項1の方法。
- 緩衝液はpH7.5ないし8.5のトリス緩衝液である請求項4の方法。
- ステップ(b)の後組織を洗浄するステップをさらに含む請求項1の方法。
- (a)生体軟組織を媒体中超高静水圧を印加することによって脱細胞するステップ、
(b)脱細胞した組織を凍結乾燥後、含まれるタンパク質を部分的に架橋固定化するため真空下100℃以上200℃の温度において加熱するステップ、
(c)部分的に架橋固定化した組織をエラスターゼと共にインキュべートし、選択的にエラスチンを除去するステップ、
を含む移植用スキャフォールドの作成方法。 - 前記軟組織は主としてコラーゲンおよびエラスチンよりなる結合組織を含み、前記スキャフォールドは主としてコラーゲンよりなる請求項7の方法。
- ステップ(a)は、5000ないし15000気圧の圧力において行われ、超高静水圧を印加して処理した組織から脱離した細胞を洗浄除去することによって行われる請求項7の方法。
- ステップ(b)の凍結乾燥後の軟組織の加熱は、少なくとも温度120℃において24時間行われる請求項7の方法。
- ステップ(c)、エラスターゼの至適pHを有する緩衝液中で実施される請求項7の方法。
- 緩衝液はpH7.5ないし8.5のトリス緩衝液である請求項11の方法。
- ステップ(c)の後組織を洗浄するステップをさらに含んでいる請求項7の方法。
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006207384A JP5050197B2 (ja) | 2006-07-31 | 2006-07-31 | 生物由来スキャフォールドの作製方法 |
US11/798,491 US7745105B2 (en) | 2006-07-31 | 2007-05-14 | Method of preparing soft tissue for a biological scaffold by lyophilizing, heating and elastase treatment |
Applications Claiming Priority (1)
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JP2006207384A JP5050197B2 (ja) | 2006-07-31 | 2006-07-31 | 生物由来スキャフォールドの作製方法 |
Publications (2)
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JP2008029653A JP2008029653A (ja) | 2008-02-14 |
JP5050197B2 true JP5050197B2 (ja) | 2012-10-17 |
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Families Citing this family (30)
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JP5675595B2 (ja) * | 2008-06-06 | 2015-02-25 | ライフセル コーポレーションLifeCell Corporation | 組織マトリックスのエラスターゼ処理 |
US8469779B1 (en) | 2009-01-02 | 2013-06-25 | Lifecell Corporation | Method for debristling animal skin |
CN107115567A (zh) * | 2009-08-18 | 2017-09-01 | 生命细胞公司 | 用于处理组织的方法 |
US8637067B1 (en) | 2011-03-10 | 2014-01-28 | Lifecell Corporation | Elastic tissue matrix derived hydrogel |
US9375513B2 (en) | 2011-04-14 | 2016-06-28 | Lifecell Corporation | Regenerative materials |
US10207025B2 (en) | 2011-04-28 | 2019-02-19 | Lifecell Corporation | Method for enzymatic treatment of tissue products |
KR102109309B1 (ko) | 2011-04-28 | 2020-05-12 | 라이프셀 코포레이션 | 조직 생성물의 효소처리방법 |
US9238793B2 (en) | 2011-04-28 | 2016-01-19 | Lifecell Corporation | Method for enzymatic treatment of tissue products |
US9089523B2 (en) | 2011-07-28 | 2015-07-28 | Lifecell Corporation | Natural tissue scaffolds as tissue fillers |
CA2859657C (en) | 2011-12-20 | 2021-03-02 | Lifecell Corporation | Acellular tissue matrix particles and flowable products comprising them |
EP2793964B1 (en) | 2011-12-20 | 2019-03-20 | LifeCell Corporation | Sheet tissue products |
US9271821B2 (en) | 2012-01-24 | 2016-03-01 | Lifecell Corporation | Elongated tissue matrices |
US9592278B2 (en) | 2012-03-08 | 2017-03-14 | Lifecell Corporation | Enzyme-activated collagen and tissue matrices |
EP2841116A1 (en) | 2012-04-24 | 2015-03-04 | Lifecell Corporation | Flowable tissue matrices |
EP2869858B1 (en) | 2012-07-06 | 2019-02-27 | LifeCell Corporation | Decellularized muscle matrix |
AU2013289045B2 (en) | 2012-07-13 | 2017-02-16 | Lifecell Corporation | Methods for improved treatment of adipose tissue |
CA2885327A1 (en) | 2012-09-26 | 2014-04-03 | Lifecell Corporation | Processed adipose tissue |
CN108042849B (zh) | 2013-02-06 | 2021-04-20 | 生命细胞公司 | 用于组织产物的局部改性的方法 |
US10307510B2 (en) | 2013-11-04 | 2019-06-04 | Lifecell Corporation | Methods of removing alpha-galactose |
AU2017274190A1 (en) | 2016-06-03 | 2018-12-13 | Lifecell Corporation | Methods for localized modification of tissue products |
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JP7463273B2 (ja) | 2017-10-19 | 2024-04-08 | ライフセル コーポレーション | 流動性無細胞組織マトリックス製品および製造方法 |
US11633521B2 (en) | 2019-05-30 | 2023-04-25 | Lifecell Corporation | Biologic breast implant |
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US110720A (en) * | 1871-01-03 | Improvement in seeding-machines | ||
JP3747412B2 (ja) * | 2000-05-26 | 2006-02-22 | エンゲルハード・リヨン | 組織工学用にデザインされた担体を製造するための水生動物起源のコラーゲンの使用並びにそれにより得られる担体及び生体材料 |
JP4092397B2 (ja) | 2002-09-10 | 2008-05-28 | 国立循環器病センター総長 | 超高静水圧印加による移植用生体組織の処理方法 |
US20060171930A1 (en) * | 2004-12-21 | 2006-08-03 | Agnieszka Seyda | Postpartum cells derived from umbilical cord tissue, and methods of making, culturing, and using the same |
JP5002805B2 (ja) * | 2005-10-14 | 2012-08-15 | 財団法人ヒューマンサイエンス振興財団 | 生物由来スキャフォールドの作製方法 |
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JP2008029653A (ja) | 2008-02-14 |
US7745105B2 (en) | 2010-06-29 |
US20080027562A1 (en) | 2008-01-31 |
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