JP2005515755A5 - - Google Patents
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- JP2005515755A5 JP2005515755A5 JP2003502145A JP2003502145A JP2005515755A5 JP 2005515755 A5 JP2005515755 A5 JP 2005515755A5 JP 2003502145 A JP2003502145 A JP 2003502145A JP 2003502145 A JP2003502145 A JP 2003502145A JP 2005515755 A5 JP2005515755 A5 JP 2005515755A5
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- JP
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- Prior art keywords
- alphavirus
- rna
- replicon
- protein
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6783939B2 (en) * | 2000-07-07 | 2004-08-31 | Alphavax, Inc. | Alphavirus vectors and virosomes with modified HIV genes for use in vaccines |
| US20030232324A1 (en) * | 2001-05-31 | 2003-12-18 | Chiron Corporation | Chimeric alphavirus replicon particles |
| PT1857122E (pt) * | 2001-06-05 | 2011-03-07 | Curevac Gmbh | Arnm estabilizado com teor de g/c aumentado, codificando para um antigénio viral |
| EP1432791B2 (en) * | 2001-09-06 | 2013-10-23 | Alphavax, Inc. | Alphavirus replicon vector systems |
| EP1585812B1 (en) * | 2002-12-13 | 2017-01-18 | Alphavax, Inc. | Multi-antigenic alphavirus replicon particles and methods |
| EP2290054B1 (en) * | 2002-12-13 | 2017-03-29 | Alphavax, Inc. | Alphavirus particles and methods for preparation |
| JP5016305B2 (ja) * | 2003-03-20 | 2012-09-05 | アルファヴァックス,インコーポレイテッド | 改良されたアルファウイルスレプリコンおよびヘルパー構築物 |
| WO2005016961A1 (en) * | 2003-06-05 | 2005-02-24 | Wyeth Holdings Corporation | Immunogenic compositions comprising venezuelan equine encephalitis virus replicon vectors and paramyxovirus protein antigens |
| ATE432285T1 (de) * | 2003-07-11 | 2009-06-15 | Alphavax Inc | Cytomegalovirusimpfstoffe, die auf dem alphavirus basieren |
| WO2005026316A2 (en) * | 2003-09-15 | 2005-03-24 | Bioption Ab | Alphavirus vaccines |
| US7622125B2 (en) | 2004-05-05 | 2009-11-24 | Novartis Vaccines And Diagnostics, Inc. | Polycistronic HIV vector constructs |
| CA2567254C (en) | 2004-05-18 | 2012-03-13 | Alphavax, Inc. | Tc-83-derived alphavirus vectors, particles and methods |
| EP1766034B1 (en) | 2004-05-21 | 2014-03-19 | Novartis Vaccines and Diagnostics, Inc. | Alphavirus vectors for influenza virus vaccines |
| US7862829B2 (en) | 2004-07-09 | 2011-01-04 | University Of North Carolina At Chapel Hill | Viral adjuvants |
| CA2585672A1 (en) | 2004-11-01 | 2006-05-11 | Novartis Vaccines And Diagnostics Inc. | Combination approaches for generating immune responses |
| EP1856250B1 (en) * | 2005-02-15 | 2013-07-24 | The University of North Carolina At Chapel Hill | New live virus vaccines |
| EP1945247A1 (en) | 2005-10-18 | 2008-07-23 | Novartis Vaccines and Diagnostics, Inc. | Mucosal and systemic immunizations with alphavirus replicon particles |
| EP1969001A2 (en) | 2005-11-22 | 2008-09-17 | Novartis Vaccines and Diagnostics, Inc. | Norovirus and sapovirus antigens |
| ES2551113T3 (es) * | 2006-01-04 | 2015-11-16 | Glaxosmithkline Biologicals S.A. | Proteína E1E2 del VHC adyuvantada con MF59 más vector de alfavirus que codifica E1E2 del VHC para provocar linfocitos T específicos del VHC |
| JP2010500399A (ja) | 2006-08-16 | 2010-01-07 | ノバルティス アーゲー | 尿路病原性大腸菌由来の免疫原 |
| US20120121650A1 (en) * | 2006-08-18 | 2012-05-17 | Johnston Robert E | Chimeric Virus Vaccines |
| PL2947149T3 (pl) * | 2007-06-21 | 2018-09-28 | Alphavax, Inc. | Kasety bez promotora do ekspresji alfawirusowych białek strukturalnych |
| WO2009048633A2 (en) * | 2007-10-11 | 2009-04-16 | The Board Of Regents Of The University Of Texas System | Chimeric chikungunya virus and uses thereof |
| WO2009049351A1 (en) | 2007-10-15 | 2009-04-23 | The University Of Queensland | Construct system and uses therefor |
| US20110229969A1 (en) * | 2008-06-25 | 2011-09-22 | Volker Sandig | Cell Line for Propagation of Highly Attenuated AlphaViruses |
| ES2902787T3 (es) | 2008-07-17 | 2022-03-29 | Medigen Inc | Vacunas de ADNi y procedimientos para utilizar las mismas |
| US9795658B2 (en) | 2010-04-20 | 2017-10-24 | Admedus Vaccines Pty Ltd | Expression system for modulating an immune response |
| EP2563909A1 (en) * | 2010-04-26 | 2013-03-06 | Novartis AG | Improved production of virus replicon particles in packaging cells |
| WO2014043518A1 (en) | 2012-09-14 | 2014-03-20 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Brachyury protein, non-poxvirus non-yeast vectors encoding brachyury protein, and their use |
| EP2971188A1 (en) * | 2013-03-15 | 2016-01-20 | The U.S.A. as represented by the Secretary, Department of Health and Human Services | Selective detection of hepatitis a, b, c, d, or e viruses or combinations thereof |
| WO2015073707A1 (en) | 2013-11-15 | 2015-05-21 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of treating heart failure with agonists of hypocretin receptor 2 |
| IL292575A (en) | 2014-09-07 | 2022-06-01 | Selecta Biosciences Inc | Methods and preparations for weakening immune responses by modulating gene expression in an antiviral transfer system |
| EP3313863B1 (en) | 2015-06-29 | 2020-12-23 | The Board of Trustees of the Leland Stanford Junior University | Degron fusion constructs and methods for controlling protein production |
| JP7048482B2 (ja) | 2015-08-03 | 2022-04-05 | アメリカ合衆国 | Brachyury欠失変異体、Brachyury欠失変異体をコードする非酵母ベクター、及びそれらの使用 |
| WO2017096432A1 (en) | 2015-12-09 | 2017-06-15 | Admedus Vaccines Pty Ltd | Immunomodulating composition for treatment |
| CN113181106A (zh) | 2016-01-11 | 2021-07-30 | 沃达瑞公司 | 微针组合物及其使用方法 |
| WO2017162265A1 (en) * | 2016-03-21 | 2017-09-28 | Biontech Rna Pharmaceuticals Gmbh | Trans-replicating rna |
| EP3472193A4 (en) | 2016-06-20 | 2020-01-08 | The Board of Trustees of the Leland Stanford Junior University | CIRCULAR RNA AND THEIR USE IN IMMUNOMODULATION |
| WO2018129268A1 (en) | 2017-01-07 | 2018-07-12 | Selecta Biosciences, Inc. | Patterned dosing of immunosuppressants coupled to synthetic nanocarriers |
| PL3601367T3 (pl) | 2017-03-30 | 2025-03-31 | The University Of Queensland | Cząsteczki chimeryczne i ich zastosowania |
| JP2020518648A (ja) | 2017-05-08 | 2020-06-25 | グリットストーン オンコロジー インコーポレイテッド | アルファウイルス新生抗原ベクター |
| EP3687561A4 (en) | 2017-09-01 | 2021-06-09 | The Australian National University | "immunoregulatory molecules and uses therefor" |
| EP3682004A4 (en) | 2017-09-15 | 2021-05-26 | The Board of Trustees of the Leland Stanford Junior University | MULTIPLEX PRODUCTION AND BARCODING OF GENETICALLY MODIFIED CELLS |
| AU2018347583A1 (en) | 2017-10-13 | 2020-05-21 | Selecta Biosciences, Inc. | Methods and compositions for attenuating anti-viral transfer vector IgM responses |
| MX2020006435A (es) | 2017-12-19 | 2021-02-09 | Akouos Inc | Administracion de anticuerpos terapeuticos mediada por vector de virus adenoasociado (aav) en el oido interno. |
| SG11202005792RA (en) | 2017-12-20 | 2020-07-29 | Vlp Therapeutics Llc | Alphavirus replicon particle |
| BR112020013679A2 (pt) | 2018-01-04 | 2020-12-01 | Iconic Therapeutics, Inc. | anticorpos de fator antitecidual, conjugados anticorpo-fármaco e métodos relacionados |
| KR20200139679A (ko) | 2018-02-26 | 2020-12-14 | 안톨알엑스, 인크. | 면역관용성 리포솜 및 그의 사용 방법 |
| BR112021006614A2 (pt) * | 2018-10-08 | 2021-07-20 | Janssen Pharmaceuticals, Inc. | replicons com base em alfavírus para administração de bioterápicos |
| CA3138525A1 (en) | 2019-04-28 | 2020-11-05 | Selecta Biosciences, Inc. | Methods for treatment of subjects with preexisting immunity to viral transfer vectors |
| AU2020284555A1 (en) | 2019-05-28 | 2021-12-23 | Selecta Biosciences, Inc. | Methods and compositions for attenuated anti-viral transfer vector immune response |
| AU2020282369A1 (en) | 2019-05-30 | 2022-01-20 | Seattle Project Corp. | Modified adenoviruses |
| EP4007814A1 (en) | 2019-07-26 | 2022-06-08 | Akouos, Inc. | Methods of treating hearing loss using a secreted target protein |
| CA3162690A1 (en) | 2019-12-31 | 2021-07-08 | Minoru S. H. Ko | Temperature-based transient delivery of nucleic acids and proteins to cells and tissues |
| CN115867658A (zh) | 2020-04-17 | 2023-03-28 | Vlp治疗公司 | 冠状病毒疫苗 |
| AU2021264216A1 (en) | 2020-04-30 | 2022-11-10 | Vlp Therapeutics, Inc. | Cytokine immunotherapy |
| EP4165194A2 (en) | 2020-05-13 | 2023-04-19 | Akouos, Inc. | Compositions and methods for treating kcnq4-associated hearing loss |
| CA3183171A1 (en) | 2020-05-13 | 2021-11-18 | Akouos, Inc. | Compositions and methods for treating slc26a4-associated hearing loss |
| WO2022032196A2 (en) | 2020-08-06 | 2022-02-10 | Gritstone Bio, Inc. | Multiepitope vaccine cassettes |
| CR20230228A (es) | 2020-12-01 | 2023-07-18 | Akouos Inc | Construcciones de anticuerpos anti-vegf y métodos relacionados para el tratamiento de los síntomas asociados al schwannoma vestibular |
| EP4271419A1 (en) | 2020-12-29 | 2023-11-08 | Akouos, Inc. | Compositions and methods for treating clrn1-associated hearing loss and/or vision loss |
| IL319926A (en) * | 2021-03-19 | 2025-05-01 | Tiba Biotech Llc | Alphavirus-derived artificial RNA replicon expression systems |
| PE20241065A1 (es) | 2021-09-30 | 2024-05-13 | Akouos Inc | Composiciones y metodos para el tratamiento de la perdida de audicion asociada a kcnq4 |
| US20230141563A1 (en) | 2021-10-12 | 2023-05-11 | Selecta Biosciences, Inc. | Methods and compositions for attenuating anti-viral transfer vector igm responses |
| WO2023070072A1 (en) | 2021-10-21 | 2023-04-27 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Retroelement-generated transcription factor decoys |
| CA3237482A1 (en) | 2021-11-03 | 2023-05-11 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Precise genome editing using retrons |
| WO2023141602A2 (en) | 2022-01-21 | 2023-07-27 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
| WO2023150142A1 (en) | 2022-02-02 | 2023-08-10 | Akouos, Inc. | Anti-vegf antibody constructs and related methods for treating vestibular schwannoma associated symptoms |
| US20230357437A1 (en) | 2022-03-09 | 2023-11-09 | Selecta Biosciences, Inc. | Immunosuppressants in combination with anti-igm agents and related dosing |
| US20250215458A1 (en) | 2022-03-25 | 2025-07-03 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David | Production of reverse transcribed DNA (RT-DNA) using a retron reverse transcriptase from exogenous RNA |
| US20250223586A1 (en) | 2022-03-25 | 2025-07-10 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David | Methods of assessing engineered retron activity, and uses thereof |
| US20250215422A1 (en) | 2022-03-25 | 2025-07-03 | The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads | RT-DNA Fidelity and Retron Genome Editing |
| WO2024009280A1 (en) | 2022-07-08 | 2024-01-11 | Baylor College Of Medicine | Integrated stress response inhibitors and methods of using the same |
| CN120344660A (zh) | 2022-07-18 | 2025-07-18 | 雷纳嘉德医疗管理公司 | 基因编辑组分、系统和使用方法 |
| US20250243516A1 (en) | 2022-08-24 | 2025-07-31 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David | Dual Cut Retron Editors for Genomic Insertions and Deletions |
| WO2024044723A1 (en) | 2022-08-25 | 2024-02-29 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
| WO2024086549A1 (en) * | 2022-10-17 | 2024-04-25 | The University Of North Carolina At Chapel Hill | Chimeric alphaviruses for directed evolution |
| WO2024229309A2 (en) | 2023-05-03 | 2024-11-07 | Manifold Biotechnologies, Inc. | Methodsand compositions for high-throughput protein delivery, screening, and detection |
| WO2025080832A1 (en) * | 2023-10-10 | 2025-04-17 | The University Of Chicago | Compositions and methods for replicon-mediated gene therapy |
| WO2025081042A1 (en) | 2023-10-12 | 2025-04-17 | Renagade Therapeutics Management Inc. | Nickase-retron template-based precision editing system and methods of use |
| WO2025155753A2 (en) | 2024-01-17 | 2025-07-24 | Renagade Therapeutics Management Inc. | Improved gene editing system, guides, and methods |
Family Cites Families (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4702909A (en) | 1982-05-05 | 1987-10-27 | Louisiana State University A & M | Non-A, non-B hepatitis antigen, antigen compositions, vaccine and diagnostic reagent |
| US4708871A (en) | 1983-03-08 | 1987-11-24 | Commonwealth Serum Laboratories Commission | Antigenically active amino acid sequences |
| US5091309A (en) | 1986-01-16 | 1992-02-25 | Washington University | Sindbis virus vectors |
| US5011915A (en) | 1987-10-26 | 1991-04-30 | Merck & Co., Inc. | Process for purifying recombinant hepatitis antigens |
| WO1989004669A1 (en) | 1987-11-18 | 1989-06-01 | Chiron Corporation | Nanbv diagnostics and vaccines |
| US5698390A (en) | 1987-11-18 | 1997-12-16 | Chiron Corporation | Hepatitis C immunoassays |
| US6184024B1 (en) * | 1988-07-14 | 2001-02-06 | The United States Of America As Represented By The Department Of Health And Human Services | Chimeric and/or growth-restricted flaviviruses |
| WO1990011089A1 (en) | 1989-03-17 | 1990-10-04 | Chiron Corporation | Nanbv diagnostics and vaccines |
| US6027729A (en) | 1989-04-20 | 2000-02-22 | Chiron Corporation | NANBV Diagnostics and vaccines |
| ATE211772T1 (de) | 1989-05-18 | 2002-01-15 | Chiron Corp | Nanbv-diagnostika: polynukleotide, geeignet für reihenuntersuchungen auf hepatitis c-virus |
| WO1990014357A1 (en) | 1989-05-19 | 1990-11-29 | Genentech, Inc. | Her2 extracellular domain |
| US5185440A (en) * | 1989-06-20 | 1993-02-09 | North Carolina State University | cDNA clone coding for Venezuelan equine encephalitis virus and attenuating mutations thereof |
| DK0444181T3 (da) | 1989-08-04 | 2002-02-25 | Schering Ag | C-erbB-2 eksternt domæne: gp75 |
| ATE152915T1 (de) | 1991-11-29 | 1997-05-15 | Viagene Inc | Immuntherapeutische vektorkonstrukte gegen krebs |
| US6297048B1 (en) | 1992-02-04 | 2001-10-02 | Chiron Corporation | Hepatitis therapeutics |
| AU3630093A (en) | 1992-03-02 | 1993-10-05 | Biocine S.P.A. | Helicobacter pylori proteins useful for vaccines and diagnostics |
| US5801005A (en) | 1993-03-17 | 1998-09-01 | University Of Washington | Immune reactivity to HER-2/neu protein for diagnosis of malignancies in which the HER-2/neu oncogene is associated |
| US5869445A (en) | 1993-03-17 | 1999-02-09 | University Of Washington | Methods for eliciting or enhancing reactivity to HER-2/neu protein |
| US6015686A (en) | 1993-09-15 | 2000-01-18 | Chiron Viagene, Inc. | Eukaryotic layered vector initiation systems |
| CA2158937C (en) * | 1993-09-15 | 2006-01-03 | Thomas W. Dubensky, Jr. | Recombinant alphavirus vectors |
| US5778245A (en) * | 1994-03-01 | 1998-07-07 | Intel Corporation | Method and apparatus for dynamic allocation of multiple buffers in a processor |
| WO1997038087A2 (en) | 1996-04-05 | 1997-10-16 | Chiron Corporation | Alphaviral vector with reduced inhibition of cellular macromolecular synthesis |
| US6451592B1 (en) | 1996-04-05 | 2002-09-17 | Chiron Corporation | Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis |
| DE19630390A1 (de) | 1996-07-26 | 1998-01-29 | Chiron Behring Gmbh & Co | Proteine, insbesondere Membranproteine von Helicobacter pylori, ihre Herstellung und Verwendung |
| US5842723A (en) | 1996-11-25 | 1998-12-01 | Burex Automotive America, Inc. | Flexible connection arrangement for connecting a pipe of an exhaust system to an engine, especially in a motor vehicle |
| PT977587E (pt) | 1997-02-28 | 2005-10-31 | Univ St Louis | Vacinas quimericas de flavivirus |
| US5935804A (en) | 1997-03-21 | 1999-08-10 | Laine; Roger A. | Method for detecting eubacteria in biological samples with catalytically inactive murein binding enzymes |
| US6261570B1 (en) * | 1997-05-20 | 2001-07-17 | The United States Of America As Represented By The Secretary Of The Army | Live attenuated virus vaccines for western equine encephalitis virus, eastern equine encephalitis virus, and venezuelan equine encephalitis virus IE and IIIA variants |
| DE69841807D1 (de) | 1997-11-06 | 2010-09-16 | Novartis Vaccines & Diagnostic | Neisseriale antigene |
| DE69941567D1 (de) | 1998-01-14 | 2009-12-03 | Novartis Vaccines & Diagnostic | Antigene aus neisseria meningitidis |
| GB9807721D0 (en) | 1998-04-08 | 1998-06-10 | Chiron Spa | Antigen |
| DE69937419T2 (de) | 1998-05-01 | 2008-07-24 | Novartis Vaccines and Diagnostics, Inc., Emeryville | Neisseria Meningitidis Antigene und Zusammenstellungen |
| WO2000039318A1 (en) | 1998-12-31 | 2000-07-06 | Chiron Corporation | Compositions and methods for packaging of alphavirus vectors |
| US6329201B1 (en) | 1998-12-31 | 2001-12-11 | Chiron Corporation | Compositions and methods for packaging of alphavirus vectors |
| DK1175497T3 (da) | 1999-04-14 | 2010-05-31 | Novartis Vaccines & Diagnostic | Præparater og fremgangsmåder til generering af et immunrespons ved udnyttelse af alfavirusbaserede vektorsystemer |
| EP1246597B1 (en) | 1999-08-03 | 2015-01-14 | The Ohio State University | Polypeptides and polynucleotides for enhancing immune reactivity to her-2 protein |
| WO2001081609A2 (en) | 2000-03-22 | 2001-11-01 | Chiron Corporation | Compositions and methods for generating an immune response utilizing alphavirus-based vector systems |
| US6865858B1 (en) | 2000-04-27 | 2005-03-15 | Marc T. Pollard | Structural protection device and method of installing the same |
| CA2410948C (en) | 2000-05-31 | 2012-07-17 | Chiron Corporation | Method for the purification of alphavirus replicon particles |
| SI1296715T2 (sl) | 2000-06-29 | 2016-03-31 | Smithkline Beecham Biologicals S.A. | Multivalentni sestavek cepiva |
| US20030232324A1 (en) | 2001-05-31 | 2003-12-18 | Chiron Corporation | Chimeric alphavirus replicon particles |
| EP2092916A1 (en) | 2008-02-19 | 2009-08-26 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | A method of treating an ocular pathology by applying high intensity focused ultrasound and device thereof |
| US9931272B2 (en) | 2010-05-21 | 2018-04-03 | Gregory Sadkhin | Method for reducing a person's weight through hunger control |
| GB2530110A (en) | 2014-11-03 | 2016-03-16 | Multi Packaging Solutions Uk Ltd | Packaging |
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2002
- 2002-04-11 JP JP2003502145A patent/JP4608210B2/ja not_active Expired - Fee Related
- 2002-04-11 AT AT02731350T patent/ATE472335T1/de active
- 2002-04-11 DE DE60236864T patent/DE60236864D1/de not_active Expired - Lifetime
- 2002-04-11 ES ES10006727.1T patent/ES2624854T3/es not_active Expired - Lifetime
- 2002-04-11 CA CA002448066A patent/CA2448066A1/en not_active Abandoned
- 2002-04-11 AU AU2002303330A patent/AU2002303330B2/en not_active Ceased
- 2002-04-11 EP EP20100179805 patent/EP2332573A1/en not_active Withdrawn
- 2002-04-11 ES ES02731350T patent/ES2345876T3/es not_active Expired - Lifetime
- 2002-04-11 EP EP02731350A patent/EP1399183B1/en not_active Expired - Lifetime
- 2002-04-11 US US10/123,101 patent/US7531180B2/en not_active Expired - Fee Related
- 2002-04-11 EP EP10006727.1A patent/EP2305299B1/en not_active Expired - Lifetime
- 2002-04-11 WO PCT/US2002/011585 patent/WO2002099035A2/en not_active Ceased
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2009
- 2009-02-17 US US12/372,295 patent/US8158418B2/en not_active Expired - Fee Related
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2010
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