JP2004110017A - 走査型レーザ顕微鏡 - Google Patents
走査型レーザ顕微鏡 Download PDFInfo
- Publication number
- JP2004110017A JP2004110017A JP2003304711A JP2003304711A JP2004110017A JP 2004110017 A JP2004110017 A JP 2004110017A JP 2003304711 A JP2003304711 A JP 2003304711A JP 2003304711 A JP2003304711 A JP 2003304711A JP 2004110017 A JP2004110017 A JP 2004110017A
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- Prior art keywords
- laser
- light
- optical system
- sample
- scanning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000003287 optical effect Effects 0.000 claims abstract description 210
- 238000010521 absorption reaction Methods 0.000 claims abstract description 41
- 230000005540 biological transmission Effects 0.000 claims abstract description 18
- 238000001228 spectrum Methods 0.000 claims abstract description 9
- 230000001678 irradiating effect Effects 0.000 claims description 4
- 230000004936 stimulating effect Effects 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 21
- 239000003153 chemical reaction reagent Substances 0.000 description 17
- 238000003384 imaging method Methods 0.000 description 16
- 238000010586 diagram Methods 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 12
- 238000002834 transmittance Methods 0.000 description 12
- 210000001747 pupil Anatomy 0.000 description 9
- 230000005284 excitation Effects 0.000 description 7
- 238000005286 illumination Methods 0.000 description 7
- 230000000903 blocking effect Effects 0.000 description 5
- 239000003269 fluorescent indicator Substances 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 4
- 238000003776 cleavage reaction Methods 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 239000000470 constituent Substances 0.000 description 2
- 238000002073 fluorescence micrograph Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- OZLGRUXZXMRXGP-UHFFFAOYSA-N Fluo-3 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=C(C=2)C2=C3C=C(Cl)C(=O)C=C3OC3=CC(O)=C(Cl)C=C32)N(CC(O)=O)CC(O)=O)=C1 OZLGRUXZXMRXGP-UHFFFAOYSA-N 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
Images
Landscapes
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003304711A JP2004110017A (ja) | 2002-08-29 | 2003-08-28 | 走査型レーザ顕微鏡 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002250824 | 2002-08-29 | ||
| JP2003304711A JP2004110017A (ja) | 2002-08-29 | 2003-08-28 | 走査型レーザ顕微鏡 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010065909A Division JP5525880B2 (ja) | 2002-08-29 | 2010-03-23 | 走査型レーザ顕微鏡 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004110017A true JP2004110017A (ja) | 2004-04-08 |
| JP2004110017A5 JP2004110017A5 (enExample) | 2006-10-12 |
Family
ID=32301283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003304711A Pending JP2004110017A (ja) | 2002-08-29 | 2003-08-28 | 走査型レーザ顕微鏡 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2004110017A (enExample) |
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005308985A (ja) * | 2004-04-20 | 2005-11-04 | Olympus Corp | 走査型レーザ顕微鏡 |
| JP2006003521A (ja) * | 2004-06-16 | 2006-01-05 | Olympus Corp | 走査型光学観察装置 |
| JP2006003747A (ja) * | 2004-06-18 | 2006-01-05 | Olympus Corp | 光走査型観察装置 |
| JP2006133747A (ja) * | 2004-10-06 | 2006-05-25 | Nikon Corp | 顕微鏡装置及びレーザユニット |
| WO2006123641A1 (ja) * | 2005-05-16 | 2006-11-23 | Olympus Corporation | 走査型観察装置 |
| JP2006343606A (ja) * | 2005-06-10 | 2006-12-21 | Yokogawa Electric Corp | 共焦点顕微鏡 |
| JP2007024943A (ja) * | 2005-07-12 | 2007-02-01 | Olympus Corp | 走査型観察装置 |
| JP2007139870A (ja) * | 2005-11-15 | 2007-06-07 | Olympus Corp | 顕微鏡装置 |
| JP2007187945A (ja) * | 2006-01-16 | 2007-07-26 | Yokogawa Electric Corp | 共焦点顕微鏡 |
| JP2007233241A (ja) * | 2006-03-03 | 2007-09-13 | Olympus Corp | 走査型レーザ顕微鏡 |
| JP2008009143A (ja) * | 2006-06-29 | 2008-01-17 | Olympus Corp | 照明装置およびレーザ走査型顕微鏡 |
| JP2008534993A (ja) * | 2005-03-26 | 2008-08-28 | カール ツァイス メディテック アクチエンゲゼルシャフト | 走査装置 |
| JP2008203417A (ja) * | 2007-02-19 | 2008-09-04 | Olympus Corp | レーザ顕微鏡 |
| DE102004044626B4 (de) * | 2004-09-13 | 2008-11-20 | Leica Microsystems Cms Gmbh | Verfahren zur Untersuchung von Transportprozessen |
| US7560709B2 (en) | 2007-02-05 | 2009-07-14 | Olympus Corporation | Scanning laser microscope |
| JP2011257599A (ja) * | 2010-06-09 | 2011-12-22 | Olympus Corp | 観察装置および観察方法 |
| JP5287252B2 (ja) * | 2006-12-22 | 2013-09-11 | 株式会社ニコン | レーザ走査共焦点顕微鏡 |
| CN103901009A (zh) * | 2014-04-22 | 2014-07-02 | 许昌学院 | 一种光纤扫描式的激光诱导荧光检测系统 |
| JP2017003863A (ja) * | 2015-06-12 | 2017-01-05 | オリンパス株式会社 | 顕微鏡システム |
| CN108427207A (zh) * | 2017-12-31 | 2018-08-21 | 浙江红谱科技有限公司 | 具有指示功能的红外图像融合系统及方法 |
| CN109724954A (zh) * | 2018-12-07 | 2019-05-07 | 北京超维景生物科技有限公司 | 荧光收集装置、微型双光子显微镜及双光子成像方法 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06160724A (ja) * | 1992-11-17 | 1994-06-07 | Nikon Corp | フラッシュフォトリシス用顕微鏡 |
| JPH07199076A (ja) * | 1993-12-27 | 1995-08-04 | Nikon Corp | コンフォーカル顕微鏡 |
| JPH09329750A (ja) * | 1996-06-11 | 1997-12-22 | Nikon Corp | 光走査型顕微鏡 |
| JPH10206742A (ja) * | 1996-11-21 | 1998-08-07 | Olympus Optical Co Ltd | レーザ走査顕微鏡 |
| JP2000275529A (ja) * | 1999-03-24 | 2000-10-06 | Olympus Optical Co Ltd | 走査型レーザ顕微鏡 |
-
2003
- 2003-08-28 JP JP2003304711A patent/JP2004110017A/ja active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06160724A (ja) * | 1992-11-17 | 1994-06-07 | Nikon Corp | フラッシュフォトリシス用顕微鏡 |
| JPH07199076A (ja) * | 1993-12-27 | 1995-08-04 | Nikon Corp | コンフォーカル顕微鏡 |
| JPH09329750A (ja) * | 1996-06-11 | 1997-12-22 | Nikon Corp | 光走査型顕微鏡 |
| JPH10206742A (ja) * | 1996-11-21 | 1998-08-07 | Olympus Optical Co Ltd | レーザ走査顕微鏡 |
| JP2000275529A (ja) * | 1999-03-24 | 2000-10-06 | Olympus Optical Co Ltd | 走査型レーザ顕微鏡 |
Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005308985A (ja) * | 2004-04-20 | 2005-11-04 | Olympus Corp | 走査型レーザ顕微鏡 |
| JP2006003521A (ja) * | 2004-06-16 | 2006-01-05 | Olympus Corp | 走査型光学観察装置 |
| JP2006003747A (ja) * | 2004-06-18 | 2006-01-05 | Olympus Corp | 光走査型観察装置 |
| US7482600B2 (en) | 2004-09-13 | 2009-01-27 | Leica Microsystems Cms Gmbh | Method for investigating transport processes |
| DE102004044626B4 (de) * | 2004-09-13 | 2008-11-20 | Leica Microsystems Cms Gmbh | Verfahren zur Untersuchung von Transportprozessen |
| JP2006133747A (ja) * | 2004-10-06 | 2006-05-25 | Nikon Corp | 顕微鏡装置及びレーザユニット |
| JP2008534993A (ja) * | 2005-03-26 | 2008-08-28 | カール ツァイス メディテック アクチエンゲゼルシャフト | 走査装置 |
| WO2006123641A1 (ja) * | 2005-05-16 | 2006-11-23 | Olympus Corporation | 走査型観察装置 |
| US7804642B2 (en) | 2005-05-16 | 2010-09-28 | Olympus Corporation | Scanning examination apparatus |
| JP2006343606A (ja) * | 2005-06-10 | 2006-12-21 | Yokogawa Electric Corp | 共焦点顕微鏡 |
| JP2007024943A (ja) * | 2005-07-12 | 2007-02-01 | Olympus Corp | 走査型観察装置 |
| US8154796B2 (en) | 2005-11-15 | 2012-04-10 | Olympus Corporation | Microscope apparatus |
| JP2007139870A (ja) * | 2005-11-15 | 2007-06-07 | Olympus Corp | 顕微鏡装置 |
| JP2007187945A (ja) * | 2006-01-16 | 2007-07-26 | Yokogawa Electric Corp | 共焦点顕微鏡 |
| JP2007233241A (ja) * | 2006-03-03 | 2007-09-13 | Olympus Corp | 走査型レーザ顕微鏡 |
| JP2008009143A (ja) * | 2006-06-29 | 2008-01-17 | Olympus Corp | 照明装置およびレーザ走査型顕微鏡 |
| JP5287252B2 (ja) * | 2006-12-22 | 2013-09-11 | 株式会社ニコン | レーザ走査共焦点顕微鏡 |
| US7560709B2 (en) | 2007-02-05 | 2009-07-14 | Olympus Corporation | Scanning laser microscope |
| JP2008203417A (ja) * | 2007-02-19 | 2008-09-04 | Olympus Corp | レーザ顕微鏡 |
| JP2011257599A (ja) * | 2010-06-09 | 2011-12-22 | Olympus Corp | 観察装置および観察方法 |
| US10001440B2 (en) | 2010-06-09 | 2018-06-19 | Olympus Corporation | Observation apparatus and observation method |
| CN103901009A (zh) * | 2014-04-22 | 2014-07-02 | 许昌学院 | 一种光纤扫描式的激光诱导荧光检测系统 |
| JP2017003863A (ja) * | 2015-06-12 | 2017-01-05 | オリンパス株式会社 | 顕微鏡システム |
| US10466459B2 (en) | 2015-06-12 | 2019-11-05 | Olympus Corporation | Microscope system |
| CN108427207A (zh) * | 2017-12-31 | 2018-08-21 | 浙江红谱科技有限公司 | 具有指示功能的红外图像融合系统及方法 |
| CN109724954A (zh) * | 2018-12-07 | 2019-05-07 | 北京超维景生物科技有限公司 | 荧光收集装置、微型双光子显微镜及双光子成像方法 |
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