JP2003511079A5 - - Google Patents
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- Publication number
- JP2003511079A5 JP2003511079A5 JP2001530502A JP2001530502A JP2003511079A5 JP 2003511079 A5 JP2003511079 A5 JP 2003511079A5 JP 2001530502 A JP2001530502 A JP 2001530502A JP 2001530502 A JP2001530502 A JP 2001530502A JP 2003511079 A5 JP2003511079 A5 JP 2003511079A5
- Authority
- JP
- Japan
- Prior art keywords
- expression vector
- protein
- polyadenylation site
- coding sequence
- vector according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000013604 expression vector Substances 0.000 description 16
- 230000008488 polyadenylation Effects 0.000 description 11
- 108091026890 Coding region Proteins 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 108020004684 Internal Ribosome Entry Sites Proteins 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 108010022394 Threonine synthase Proteins 0.000 description 1
- 102000004419 dihydrofolate reductase Human genes 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15917799P | 1999-10-13 | 1999-10-13 | |
| US60/159,177 | 1999-10-13 | ||
| PCT/US2000/028431 WO2001027299A1 (en) | 1999-10-13 | 2000-10-12 | Vectors and methods for recombinant protein expression |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003511079A JP2003511079A (ja) | 2003-03-25 |
| JP2003511079A5 true JP2003511079A5 (https=) | 2007-11-29 |
| JP4660046B2 JP4660046B2 (ja) | 2011-03-30 |
Family
ID=22571409
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001530502A Expired - Lifetime JP4660046B2 (ja) | 1999-10-13 | 2000-10-12 | 組換えタンパク質発現のためのベクターおよび方法 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US6632637B1 (https=) |
| EP (1) | EP1222298B1 (https=) |
| JP (1) | JP4660046B2 (https=) |
| AT (1) | ATE312186T1 (https=) |
| AU (1) | AU774068B2 (https=) |
| CA (1) | CA2386180C (https=) |
| CY (1) | CY1105336T1 (https=) |
| DE (1) | DE60024659T2 (https=) |
| DK (1) | DK1222298T3 (https=) |
| ES (1) | ES2252070T3 (https=) |
| IL (2) | IL148785A0 (https=) |
| NZ (1) | NZ518388A (https=) |
| WO (1) | WO2001027299A1 (https=) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050005310A1 (en) * | 1999-07-12 | 2005-01-06 | Genentech, Inc. | Expression vectors and methods |
| US6632637B1 (en) * | 1999-10-13 | 2003-10-14 | Immunex Corporation | Vectors and methods for recombinant protein expression |
| WO2003035887A1 (en) * | 2001-09-20 | 2003-05-01 | Immunex Corporation | Selection of cells expressing heteromeric polypeptides |
| US20030157641A1 (en) * | 2001-11-16 | 2003-08-21 | Idec Pharmaceuticals Corporation | Polycistronic expression of antibodies |
| CA2480121C (en) | 2002-03-27 | 2012-02-28 | Immunex Corporation | Methods for increasing polypeptide production |
| WO2004046340A2 (en) * | 2002-11-14 | 2004-06-03 | Genentech, Inc. | Intron fusion construct and method of using for selecting high-expressing production cell lines |
| KR100533794B1 (ko) * | 2003-10-02 | 2005-12-07 | 주식회사 프로젠 | 인간 난포자극 호르몬을 대량으로 생산하는 방법 |
| WO2005123915A1 (en) * | 2004-06-21 | 2005-12-29 | Novozymes A/S | Stably maintained multiple copies of at least two orf in the same orientation |
| WO2006012414A2 (en) * | 2004-07-20 | 2006-02-02 | Critical Therapeutics, Inc. | Novel polyadenylation signal for use in expression vectors |
| JPWO2006093053A1 (ja) * | 2005-03-02 | 2008-08-07 | アステラス製薬株式会社 | ヒストンデアセチラーゼ阻害剤の新規ｐｄマーカー |
| MX2008000985A (es) | 2005-07-21 | 2008-04-07 | Abbott Lab | Expresion de gen multiple que incluye construcciones y metodos sorf (cuadro de lectura abierta simple) con poliproteinas, pro-proteinas y proteolisis. |
| AU2007294731B2 (en) | 2006-09-13 | 2014-04-17 | Abbvie Inc. | Cell culture improvements |
| EP2500416A1 (en) | 2006-09-13 | 2012-09-19 | Abbott Laboratories | Cell culture improvements |
| CA2716801A1 (en) * | 2008-03-12 | 2009-09-17 | Wyeth Llc | Methods for identifying cells suitable for large-scale production of recombinant proteins |
| WO2010114270A2 (ko) | 2009-03-31 | 2010-10-07 | (주)셀트리온 | 고발현 재조합 세포주의 선별 방법 |
| WO2011053699A1 (en) * | 2009-10-30 | 2011-05-05 | Abbott Laboratories | Sorf constructs and multiple gene expression |
| WO2011113841A1 (en) * | 2010-03-16 | 2011-09-22 | Robert Steinfeld | Eukaryotic vector |
| WO2012018607A2 (en) * | 2010-07-26 | 2012-02-09 | Adv Biologics, Inc. | Vectors and methods for recombinant protein expression |
| CN103201382A (zh) * | 2010-11-08 | 2013-07-10 | 日本化学研究株式会社 | 新表达载体 |
| IL210093A0 (en) * | 2010-12-19 | 2011-06-30 | David Helman | Membrane bound reporter molecules and their use in cell sorting |
| JP6463724B2 (ja) | 2013-03-15 | 2019-02-06 | バイエル クロップサイエンス エルピーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ｌｐ | 構成ダイズプロモーター |
| AU2015243535A1 (en) | 2014-04-08 | 2016-11-03 | University Of Southern California | Polypeptide compositions with Type VII collagen fibronectin Type Ill- like repeats and treatment methods for wound closure and healing |
| CN107043773B (zh) * | 2017-02-08 | 2018-09-18 | 四川丰讯科技发展有限公司 | 一种提高哺乳动物细胞中外源基因表达水平的基因片段、重组载体及其用途 |
| CA3117961A1 (en) | 2018-10-29 | 2020-05-07 | Spin Therapeutics, Llc | Compositions and methods for alpha-1-antitrypsin disorders |
| KR102490457B1 (ko) * | 2019-11-26 | 2023-01-19 | 한국생명공학연구원 | 단백질 발현용 mRNA 구조체 및 이의 용도 |
| KR20230124578A (ko) | 2020-12-22 | 2023-08-25 | 암젠 인크 | 세포 배양 방법 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965196A (en) * | 1983-01-19 | 1990-10-23 | Genentech, Inc. | Polycistronic expression vector construction |
| US4937190A (en) * | 1987-10-15 | 1990-06-26 | Wisconsin Alumni Research Foundation | Translation enhancer |
| EP0555203B1 (en) * | 1988-12-08 | 1995-10-18 | Abbott Laboratories | Expression induction method employing dhfr gene |
| EP0662128B1 (en) * | 1992-09-28 | 2005-11-23 | Chiron Corporation | Methods and compositions for controlling translation of hcv proteins |
| US5698446A (en) * | 1994-09-07 | 1997-12-16 | Chiron Corporation | Methods and compositions for inhibiting production of replication competent virus |
| DE19514310A1 (de) * | 1995-04-18 | 1996-10-24 | Univ Ludwigs Albert | Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen |
| GB9517263D0 (en) | 1995-08-23 | 1995-10-25 | Cancer Res Campaign Tech | Expression systems |
| ES2227669T3 (es) | 1996-01-11 | 2005-04-01 | Immunex Corporation | Elementos de secuencia que aumentan la expresion (ease) para sistemas de expresion de eurocariotas. |
| EP0956301A2 (en) * | 1996-10-18 | 1999-11-17 | Valentis Inc. | Il-12 gene expression and delivery systems and uses |
| US6632637B1 (en) * | 1999-10-13 | 2003-10-14 | Immunex Corporation | Vectors and methods for recombinant protein expression |
-
2000
- 2000-10-12 US US09/687,050 patent/US6632637B1/en not_active Expired - Lifetime
- 2000-10-12 WO PCT/US2000/028431 patent/WO2001027299A1/en not_active Ceased
- 2000-10-12 ES ES00970900T patent/ES2252070T3/es not_active Expired - Lifetime
- 2000-10-12 NZ NZ518388A patent/NZ518388A/en unknown
- 2000-10-12 JP JP2001530502A patent/JP4660046B2/ja not_active Expired - Lifetime
- 2000-10-12 DE DE60024659T patent/DE60024659T2/de not_active Expired - Lifetime
- 2000-10-12 CA CA002386180A patent/CA2386180C/en not_active Expired - Fee Related
- 2000-10-12 EP EP00970900A patent/EP1222298B1/en not_active Expired - Lifetime
- 2000-10-12 IL IL14878500A patent/IL148785A0/xx active IP Right Grant
- 2000-10-12 AT AT00970900T patent/ATE312186T1/de not_active IP Right Cessation
- 2000-10-12 AU AU80216/00A patent/AU774068B2/en not_active Ceased
- 2000-10-12 DK DK00970900T patent/DK1222298T3/da active
-
2002
- 2002-03-20 IL IL148785A patent/IL148785A/en not_active IP Right Cessation
-
2003
- 2003-10-10 US US10/683,775 patent/US20040063186A1/en not_active Abandoned
-
2006
- 2006-02-22 CY CY20061100258T patent/CY1105336T1/el unknown
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