JP4660046B2 - 組換えタンパク質発現のためのベクターおよび方法 - Google Patents

組換えタンパク質発現のためのベクターおよび方法 Download PDF

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Publication number
JP4660046B2
JP4660046B2 JP2001530502A JP2001530502A JP4660046B2 JP 4660046 B2 JP4660046 B2 JP 4660046B2 JP 2001530502 A JP2001530502 A JP 2001530502A JP 2001530502 A JP2001530502 A JP 2001530502A JP 4660046 B2 JP4660046 B2 JP 4660046B2
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protein
expression vector
coding sequence
cells
expression
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Japanese (ja)
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JP2003511079A5 (https=
JP2003511079A (ja
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マクグルー，ジェフリー・ティー
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Immunex Corp
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Immunex Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2001530502A 1999-10-13 2000-10-12 組換えタンパク質発現のためのベクターおよび方法 Expired - Lifetime JP4660046B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15917799P 1999-10-13 1999-10-13
US60/159,177 1999-10-13
PCT/US2000/028431 WO2001027299A1 (en) 1999-10-13 2000-10-12 Vectors and methods for recombinant protein expression

Publications (3)

Publication Number Publication Date
JP2003511079A JP2003511079A (ja) 2003-03-25
JP2003511079A5 JP2003511079A5 (https=) 2007-11-29
JP4660046B2 true JP4660046B2 (ja) 2011-03-30

Family

ID=22571409

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2001530502A Expired - Lifetime JP4660046B2 (ja) 1999-10-13 2000-10-12 組換えタンパク質発現のためのベクターおよび方法

Country Status (13)

Country Link
US (2) US6632637B1 (https=)
EP (1) EP1222298B1 (https=)
JP (1) JP4660046B2 (https=)
AT (1) ATE312186T1 (https=)
AU (1) AU774068B2 (https=)
CA (1) CA2386180C (https=)
CY (1) CY1105336T1 (https=)
DE (1) DE60024659T2 (https=)
DK (1) DK1222298T3 (https=)
ES (1) ES2252070T3 (https=)
IL (2) IL148785A0 (https=)
NZ (1) NZ518388A (https=)
WO (1) WO2001027299A1 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050005310A1 (en) * 1999-07-12 2005-01-06 Genentech, Inc. Expression vectors and methods
US6632637B1 (en) * 1999-10-13 2003-10-14 Immunex Corporation Vectors and methods for recombinant protein expression
WO2003035887A1 (en) * 2001-09-20 2003-05-01 Immunex Corporation Selection of cells expressing heteromeric polypeptides
US20030157641A1 (en) * 2001-11-16 2003-08-21 Idec Pharmaceuticals Corporation Polycistronic expression of antibodies
CA2480121C (en) 2002-03-27 2012-02-28 Immunex Corporation Methods for increasing polypeptide production
WO2004046340A2 (en) * 2002-11-14 2004-06-03 Genentech, Inc. Intron fusion construct and method of using for selecting high-expressing production cell lines
KR100533794B1 (ko) * 2003-10-02 2005-12-07 주식회사 프로젠 인간 난포자극 호르몬을 대량으로 생산하는 방법
WO2005123915A1 (en) * 2004-06-21 2005-12-29 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
WO2006012414A2 (en) * 2004-07-20 2006-02-02 Critical Therapeutics, Inc. Novel polyadenylation signal for use in expression vectors
JPWO2006093053A1 (ja) * 2005-03-02 2008-08-07 アステラス製薬株式会社 ヒストンデアセチラーゼ阻害剤の新規ｐｄマーカー
MX2008000985A (es) 2005-07-21 2008-04-07 Abbott Lab Expresion de gen multiple que incluye construcciones y metodos sorf (cuadro de lectura abierta simple) con poliproteinas, pro-proteinas y proteolisis.
AU2007294731B2 (en) 2006-09-13 2014-04-17 Abbvie Inc. Cell culture improvements
EP2500416A1 (en) 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
CA2716801A1 (en) * 2008-03-12 2009-09-17 Wyeth Llc Methods for identifying cells suitable for large-scale production of recombinant proteins
WO2010114270A2 (ko) 2009-03-31 2010-10-07 (주)셀트리온 고발현 재조합 세포주의 선별 방법
WO2011053699A1 (en) * 2009-10-30 2011-05-05 Abbott Laboratories Sorf constructs and multiple gene expression
WO2011113841A1 (en) * 2010-03-16 2011-09-22 Robert Steinfeld Eukaryotic vector
WO2012018607A2 (en) * 2010-07-26 2012-02-09 Adv Biologics, Inc. Vectors and methods for recombinant protein expression
CN103201382A (zh) * 2010-11-08 2013-07-10 日本化学研究株式会社 新表达载体
IL210093A0 (en) * 2010-12-19 2011-06-30 David Helman Membrane bound reporter molecules and their use in cell sorting
JP6463724B2 (ja) 2013-03-15 2019-02-06 バイエル クロップサイエンス エルピーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ｌｐ 構成ダイズプロモーター
AU2015243535A1 (en) 2014-04-08 2016-11-03 University Of Southern California Polypeptide compositions with Type VII collagen fibronectin Type Ill- like repeats and treatment methods for wound closure and healing
CN107043773B (zh) * 2017-02-08 2018-09-18 四川丰讯科技发展有限公司 一种提高哺乳动物细胞中外源基因表达水平的基因片段、重组载体及其用途
CA3117961A1 (en) 2018-10-29 2020-05-07 Spin Therapeutics, Llc Compositions and methods for alpha-1-antitrypsin disorders
KR102490457B1 (ko) * 2019-11-26 2023-01-19 한국생명공학연구원 단백질 발현용 mRNA 구조체 및 이의 용도
KR20230124578A (ko) 2020-12-22 2023-08-25 암젠 인크 세포 배양 방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965196A (en) * 1983-01-19 1990-10-23 Genentech, Inc. Polycistronic expression vector construction
US4937190A (en) * 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
EP0555203B1 (en) * 1988-12-08 1995-10-18 Abbott Laboratories Expression induction method employing dhfr gene
EP0662128B1 (en) * 1992-09-28 2005-11-23 Chiron Corporation Methods and compositions for controlling translation of hcv proteins
US5698446A (en) * 1994-09-07 1997-12-16 Chiron Corporation Methods and compositions for inhibiting production of replication competent virus
DE19514310A1 (de) * 1995-04-18 1996-10-24 Univ Ludwigs Albert Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen
GB9517263D0 (en) 1995-08-23 1995-10-25 Cancer Res Campaign Tech Expression systems
ES2227669T3 (es) 1996-01-11 2005-04-01 Immunex Corporation Elementos de secuencia que aumentan la expresion (ease) para sistemas de expresion de eurocariotas.
EP0956301A2 (en) * 1996-10-18 1999-11-17 Valentis Inc. Il-12 gene expression and delivery systems and uses
US6632637B1 (en) * 1999-10-13 2003-10-14 Immunex Corporation Vectors and methods for recombinant protein expression

Also Published As

Publication number Publication date
US6632637B1 (en) 2003-10-14
IL148785A0 (en) 2002-09-12
IL148785A (en) 2007-09-20
CA2386180A1 (en) 2001-04-19
AU774068B2 (en) 2004-06-17
AU8021600A (en) 2001-04-23
EP1222298B1 (en) 2005-12-07
WO2001027299A1 (en) 2001-04-19
CA2386180C (en) 2010-03-09
DK1222298T3 (da) 2006-04-10
DE60024659T2 (de) 2006-08-17
EP1222298A1 (en) 2002-07-17
JP2003511079A (ja) 2003-03-25
NZ518388A (en) 2003-10-31
US20040063186A1 (en) 2004-04-01
CY1105336T1 (el) 2010-03-03
ATE312186T1 (de) 2005-12-15
DE60024659D1 (de) 2006-01-12
ES2252070T3 (es) 2006-05-16

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