DE60024659T2 - Vektoren und verfahren für rekombinante proteinexpression - Google Patents

Vektoren und verfahren für rekombinante proteinexpression Download PDF

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Publication number
DE60024659T2
DE60024659T2 DE60024659T DE60024659T DE60024659T2 DE 60024659 T2 DE60024659 T2 DE 60024659T2 DE 60024659 T DE60024659 T DE 60024659T DE 60024659 T DE60024659 T DE 60024659T DE 60024659 T2 DE60024659 T2 DE 60024659T2
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DE
Germany
Prior art keywords
coding sequence
expression vector
expression
protein
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60024659T
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German (de)
English (en)
Other versions
DE60024659D1 (de
Inventor
T. Jeffrey MCGREW
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immunex Corp
Original Assignee
Immunex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22571409&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60024659(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Immunex Corp filed Critical Immunex Corp
Application granted granted Critical
Publication of DE60024659D1 publication Critical patent/DE60024659D1/de
Publication of DE60024659T2 publication Critical patent/DE60024659T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE60024659T 1999-10-13 2000-10-12 Vektoren und verfahren für rekombinante proteinexpression Expired - Lifetime DE60024659T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15917799P 1999-10-13 1999-10-13
US159177P 1999-10-13
PCT/US2000/028431 WO2001027299A1 (en) 1999-10-13 2000-10-12 Vectors and methods for recombinant protein expression

Publications (2)

Publication Number Publication Date
DE60024659D1 DE60024659D1 (de) 2006-01-12
DE60024659T2 true DE60024659T2 (de) 2006-08-17

Family

ID=22571409

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60024659T Expired - Lifetime DE60024659T2 (de) 1999-10-13 2000-10-12 Vektoren und verfahren für rekombinante proteinexpression

Country Status (13)

Country Link
US (2) US6632637B1 (https=)
EP (1) EP1222298B1 (https=)
JP (1) JP4660046B2 (https=)
AT (1) ATE312186T1 (https=)
AU (1) AU774068B2 (https=)
CA (1) CA2386180C (https=)
CY (1) CY1105336T1 (https=)
DE (1) DE60024659T2 (https=)
DK (1) DK1222298T3 (https=)
ES (1) ES2252070T3 (https=)
IL (2) IL148785A0 (https=)
NZ (1) NZ518388A (https=)
WO (1) WO2001027299A1 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050005310A1 (en) * 1999-07-12 2005-01-06 Genentech, Inc. Expression vectors and methods
US6632637B1 (en) * 1999-10-13 2003-10-14 Immunex Corporation Vectors and methods for recombinant protein expression
WO2003035887A1 (en) * 2001-09-20 2003-05-01 Immunex Corporation Selection of cells expressing heteromeric polypeptides
US20030157641A1 (en) * 2001-11-16 2003-08-21 Idec Pharmaceuticals Corporation Polycistronic expression of antibodies
CA2480121C (en) 2002-03-27 2012-02-28 Immunex Corporation Methods for increasing polypeptide production
WO2004046340A2 (en) * 2002-11-14 2004-06-03 Genentech, Inc. Intron fusion construct and method of using for selecting high-expressing production cell lines
KR100533794B1 (ko) * 2003-10-02 2005-12-07 주식회사 프로젠 인간 난포자극 호르몬을 대량으로 생산하는 방법
WO2005123915A1 (en) * 2004-06-21 2005-12-29 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
WO2006012414A2 (en) * 2004-07-20 2006-02-02 Critical Therapeutics, Inc. Novel polyadenylation signal for use in expression vectors
JPWO2006093053A1 (ja) * 2005-03-02 2008-08-07 アステラス製薬株式会社 ヒストンデアセチラーゼ阻害剤の新規ｐｄマーカー
MX2008000985A (es) 2005-07-21 2008-04-07 Abbott Lab Expresion de gen multiple que incluye construcciones y metodos sorf (cuadro de lectura abierta simple) con poliproteinas, pro-proteinas y proteolisis.
AU2007294731B2 (en) 2006-09-13 2014-04-17 Abbvie Inc. Cell culture improvements
EP2500416A1 (en) 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
CA2716801A1 (en) * 2008-03-12 2009-09-17 Wyeth Llc Methods for identifying cells suitable for large-scale production of recombinant proteins
WO2010114270A2 (ko) 2009-03-31 2010-10-07 (주)셀트리온 고발현 재조합 세포주의 선별 방법
WO2011053699A1 (en) * 2009-10-30 2011-05-05 Abbott Laboratories Sorf constructs and multiple gene expression
WO2011113841A1 (en) * 2010-03-16 2011-09-22 Robert Steinfeld Eukaryotic vector
WO2012018607A2 (en) * 2010-07-26 2012-02-09 Adv Biologics, Inc. Vectors and methods for recombinant protein expression
CN103201382A (zh) * 2010-11-08 2013-07-10 日本化学研究株式会社 新表达载体
IL210093A0 (en) * 2010-12-19 2011-06-30 David Helman Membrane bound reporter molecules and their use in cell sorting
JP6463724B2 (ja) 2013-03-15 2019-02-06 バイエル クロップサイエンス エルピーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ｌｐ 構成ダイズプロモーター
AU2015243535A1 (en) 2014-04-08 2016-11-03 University Of Southern California Polypeptide compositions with Type VII collagen fibronectin Type Ill- like repeats and treatment methods for wound closure and healing
CN107043773B (zh) * 2017-02-08 2018-09-18 四川丰讯科技发展有限公司 一种提高哺乳动物细胞中外源基因表达水平的基因片段、重组载体及其用途
CA3117961A1 (en) 2018-10-29 2020-05-07 Spin Therapeutics, Llc Compositions and methods for alpha-1-antitrypsin disorders
KR102490457B1 (ko) * 2019-11-26 2023-01-19 한국생명공학연구원 단백질 발현용 mRNA 구조체 및 이의 용도
KR20230124578A (ko) 2020-12-22 2023-08-25 암젠 인크 세포 배양 방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965196A (en) * 1983-01-19 1990-10-23 Genentech, Inc. Polycistronic expression vector construction
US4937190A (en) * 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
EP0555203B1 (en) * 1988-12-08 1995-10-18 Abbott Laboratories Expression induction method employing dhfr gene
EP0662128B1 (en) * 1992-09-28 2005-11-23 Chiron Corporation Methods and compositions for controlling translation of hcv proteins
US5698446A (en) * 1994-09-07 1997-12-16 Chiron Corporation Methods and compositions for inhibiting production of replication competent virus
DE19514310A1 (de) * 1995-04-18 1996-10-24 Univ Ludwigs Albert Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen
GB9517263D0 (en) 1995-08-23 1995-10-25 Cancer Res Campaign Tech Expression systems
ES2227669T3 (es) 1996-01-11 2005-04-01 Immunex Corporation Elementos de secuencia que aumentan la expresion (ease) para sistemas de expresion de eurocariotas.
EP0956301A2 (en) * 1996-10-18 1999-11-17 Valentis Inc. Il-12 gene expression and delivery systems and uses
US6632637B1 (en) * 1999-10-13 2003-10-14 Immunex Corporation Vectors and methods for recombinant protein expression

Also Published As

Publication number Publication date
US6632637B1 (en) 2003-10-14
IL148785A0 (en) 2002-09-12
IL148785A (en) 2007-09-20
CA2386180A1 (en) 2001-04-19
AU774068B2 (en) 2004-06-17
AU8021600A (en) 2001-04-23
EP1222298B1 (en) 2005-12-07
WO2001027299A1 (en) 2001-04-19
CA2386180C (en) 2010-03-09
DK1222298T3 (da) 2006-04-10
JP4660046B2 (ja) 2011-03-30
EP1222298A1 (en) 2002-07-17
JP2003511079A (ja) 2003-03-25
NZ518388A (en) 2003-10-31
US20040063186A1 (en) 2004-04-01
CY1105336T1 (el) 2010-03-03
ATE312186T1 (de) 2005-12-15
DE60024659D1 (de) 2006-01-12
ES2252070T3 (es) 2006-05-16

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