DE69030740T2 - Rekombinantes DNS-Verfahren und Wirtszellen - Google Patents

Rekombinantes DNS-Verfahren und Wirtszellen

Info

Publication number
DE69030740T2
DE69030740T2 DE69030740T DE69030740T DE69030740T2 DE 69030740 T2 DE69030740 T2 DE 69030740T2 DE 69030740 T DE69030740 T DE 69030740T DE 69030740 T DE69030740 T DE 69030740T DE 69030740 T2 DE69030740 T2 DE 69030740T2
Authority
DE
Germany
Prior art keywords
host cells
recombinant dna
dna method
amount
transcription unit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69030740T
Other languages
English (en)
Other versions
DE69030740D1 (de
Inventor
Mark Ian Cockett
Christopher Robert Bebbington
Geoffrey Thomas Yarranton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza Group AG
Original Assignee
Celltech R&D Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celltech R&D Ltd filed Critical Celltech R&D Ltd
Application granted granted Critical
Publication of DE69030740D1 publication Critical patent/DE69030740D1/de
Publication of DE69030740T2 publication Critical patent/DE69030740T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
DE69030740T 1989-01-10 1990-01-10 Rekombinantes DNS-Verfahren und Wirtszellen Expired - Lifetime DE69030740T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898900483A GB8900483D0 (en) 1989-01-10 1989-01-10 Recombinant dna method

Publications (2)

Publication Number Publication Date
DE69030740D1 DE69030740D1 (de) 1997-06-26
DE69030740T2 true DE69030740T2 (de) 1997-11-27

Family

ID=10649831

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69030740T Expired - Lifetime DE69030740T2 (de) 1989-01-10 1990-01-10 Rekombinantes DNS-Verfahren und Wirtszellen

Country Status (12)

Country Link
US (2) US5866359A (de)
EP (1) EP0378382B1 (de)
JP (1) JP3070950B2 (de)
AT (1) ATE153382T1 (de)
AU (2) AU4825390A (de)
CA (1) CA2024607C (de)
DE (1) DE69030740T2 (de)
DK (1) DK0378382T3 (de)
ES (1) ES2103266T3 (de)
GB (1) GB8900483D0 (de)
GR (1) GR3024002T3 (de)
WO (1) WO1990008191A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8900483D0 (en) 1989-01-10 1989-03-08 Celltech Ltd Recombinant dna method
EP0455424A3 (en) * 1990-05-02 1992-04-29 Merck & Co. Inc. Mammalian inducible promoter cascade system
CA2047633A1 (en) * 1990-09-12 1992-03-13 Ben Bowen Inactivation of gene transcription in plants using altered transcriptional activators
PT566554E (pt) 1992-03-13 2000-09-29 Monsanto Co Producao de proteinas recombinantes usando promotores do virus de herpes e transactivadores vp16
US5585237A (en) * 1993-10-25 1996-12-17 Creative Biomolecules, Inc. Methods and compositions for high protein production from recombinant DNA
US5733782A (en) * 1993-10-25 1998-03-31 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA
DE69638058D1 (de) 1995-06-15 2009-11-26 Crucell Holland Bv Verpackungssysteme für humane rekombinante Adenoviren zur Gentherapie
US7297680B2 (en) * 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US7604960B2 (en) * 1999-04-15 2009-10-20 Crucell Holland B.V. Transient protein expression methods
US8236561B2 (en) * 1999-04-15 2012-08-07 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US6855544B1 (en) * 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
US20050164386A1 (en) * 1999-04-15 2005-07-28 Uytdehaag Alphonsus G. Overexpression of enzymes involved in post-translational protein modifications in human cells
SI1161548T2 (sl) * 1999-04-15 2010-02-26 Crucell Holland Bv Priprava rekombinantnega proteina v humani celici z uporabo sekvenc, ki kodirajo adenovirusni E1 protein
US20050170463A1 (en) * 1999-04-15 2005-08-04 Abraham Bout Recombinant protein production in permanent amniocytic cells that comprise nucleic acid encoding adenovirus E1A and E1B proteins
US7527961B2 (en) * 1999-11-26 2009-05-05 Crucell Holland B.V. Production of vaccines
US7521220B2 (en) * 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
US7192759B1 (en) * 1999-11-26 2007-03-20 Crucell Holland B.V. Production of vaccines
US20030119104A1 (en) * 2001-05-30 2003-06-26 Edward Perkins Chromosome-based platforms
ATE384785T1 (de) * 2001-12-07 2008-02-15 Crucell Holland Bv Herstellung von viren, virusisolaten, und impfstoffen
JP4865539B2 (ja) 2003-05-09 2012-02-01 クルセル ホランド ベー ヴェー Ｅ１不死化細胞の培養物及び該培養物を培養して該培養物から得られる産物の収量を増加させる方法
AU2004240782B2 (en) * 2003-05-23 2011-04-21 Crucell Holland B.V. Production of recombinant IGM in per.C6 cells
US8354251B2 (en) 2003-12-31 2013-01-15 Kalobios Pharmaceuticals, Inc. Transactivation system for mammalian cells
PL2711426T3 (pl) 2012-09-24 2015-09-30 Lonza Biologics Plc Wektory ekspresyjne zawierające chimeryczne sekwencje promotora i wzmacniacza wirusa cytomegalii
EP2829608A1 (de) 2013-07-23 2015-01-28 Universität Bielefeld Verfahren zur Herstellung von rekombinanten Proteinen in Säugerzellen

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
EP0245949B1 (de) * 1986-04-09 1997-10-29 Eli Lilly And Company Verfahren zur Anwendung von den BK-Virus-Verstärker enthaltenden eukaryotischen Expressionsvektoren
US4775630A (en) * 1986-08-15 1988-10-04 Vanderbilt University Transcriptional control element adapted for regulation of gene expression in animal cells
EP0282330A3 (de) * 1987-03-13 1988-11-09 Schering Corporation Verfahren zur Stimulierung der Genexpression
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
GB8900483D0 (en) 1989-01-10 1989-03-08 Celltech Ltd Recombinant dna method

Also Published As

Publication number Publication date
ES2103266T3 (es) 1997-09-16
GR3024002T3 (en) 1997-10-31
EP0378382B1 (de) 1997-05-21
DK0378382T3 (da) 1997-06-16
ATE153382T1 (de) 1997-06-15
WO1990008191A1 (en) 1990-07-26
CA2024607A1 (en) 1990-07-11
JPH03503959A (ja) 1991-09-05
AU3994193A (en) 1993-08-12
CA2024607C (en) 1999-11-30
US5866359A (en) 1999-02-02
US6653101B1 (en) 2003-11-25
DE69030740D1 (de) 1997-06-26
AU4825390A (en) 1990-08-13
JP3070950B2 (ja) 2000-07-31
EP0378382A1 (de) 1990-07-18
AU662416B2 (en) 1995-08-31
GB8900483D0 (en) 1989-03-08

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: ALUSUISSE HOLDINGS A.G., NEUHAUSEN AM RHEINFALL, C

8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: LONZA GROUP AG, ZUERICH, CH