IL162302A0 - Methods for producing hyaluronan ina recombinant host cell - Google Patents

Methods for producing hyaluronan ina recombinant host cell

Info

Publication number
IL162302A0
IL162302A0 IL16230202A IL16230202A IL162302A0 IL 162302 A0 IL162302 A0 IL 162302A0 IL 16230202 A IL16230202 A IL 16230202A IL 16230202 A IL16230202 A IL 16230202A IL 162302 A0 IL162302 A0 IL 162302A0
Authority
IL
Israel
Prior art keywords
udp
glucose
gene
host cell
hyaluronan synthase
Prior art date
Application number
IL16230202A
Other languages
English (en)
Original Assignee
Novozymes Biopolymer As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biopolymer As filed Critical Novozymes Biopolymer As
Publication of IL162302A0 publication Critical patent/IL162302A0/xx

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
IL16230202A 2001-12-21 2002-12-20 Methods for producing hyaluronan ina recombinant host cell IL162302A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34264401P 2001-12-21 2001-12-21
PCT/US2002/041067 WO2003054163A2 (en) 2001-12-21 2002-12-20 Methods for producing hyaluronan in a recombinant host cell

Publications (1)

Publication Number Publication Date
IL162302A0 true IL162302A0 (en) 2005-11-20

Family

ID=23342668

Family Applications (2)

Application Number Title Priority Date Filing Date
IL16230202A IL162302A0 (en) 2001-12-21 2002-12-20 Methods for producing hyaluronan ina recombinant host cell
IL162302A IL162302A (en) 2001-12-21 2004-06-02 Methods of producing hyaluronic acid in a bacillus host cell

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL162302A IL162302A (en) 2001-12-21 2004-06-02 Methods of producing hyaluronic acid in a bacillus host cell

Country Status (18)

Country Link
US (5) US7811806B2 (hu)
EP (1) EP1572895B1 (hu)
JP (1) JP2005525091A (hu)
KR (2) KR100879908B1 (hu)
CN (1) CN1636052B (hu)
AT (1) ATE502115T1 (hu)
AU (1) AU2002366711C1 (hu)
BR (1) BR0215220A (hu)
CA (2) CA2471148C (hu)
CZ (1) CZ2004765A3 (hu)
DE (1) DE60239495D1 (hu)
DK (1) DK1572895T3 (hu)
ES (1) ES2362354T3 (hu)
HU (2) HU228693B1 (hu)
IL (2) IL162302A0 (hu)
PL (2) PL212928B1 (hu)
RU (1) RU2346049C2 (hu)
WO (1) WO2003054163A2 (hu)

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DE69828193T2 (de) * 1997-10-31 2005-12-01 The Board Of Regents Of The University Of Oklahoma, Norman Hyaluronan synthase gen und seine verwendung
ATE470708T1 (de) * 2001-06-13 2010-06-15 Univ Oklahoma State Hyaluronansynthase-gene und expression davon
WO2003054163A2 (en) * 2001-12-21 2003-07-03 Novozymes Biopolymer A/S Methods for producing hyaluronan in a recombinant host cell
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WO2004011609A2 (en) * 2002-07-26 2004-02-05 Novozymes Biotech, Inc. Methods for producing biological substances in pigment-deficient mutants of bacillus cells
US7494798B2 (en) * 2004-01-09 2009-02-24 Novozymes, Inc. Bacillus licheniformis chromosome
US20050221446A1 (en) * 2004-03-31 2005-10-06 Novozymes Biopolymer A/S. Methods for producing hyaluronic acid in a Bacillus cell
US7002007B2 (en) * 2004-05-28 2006-02-21 Calcigen Corporation Production of high molecular weight hyaluronates
US20080044853A1 (en) 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
EP1640457A1 (de) * 2004-09-23 2006-03-29 Bayer CropScience GmbH Verfahren und Mittel zur Herstellung von Hyaluronan
SI1805312T1 (sl) * 2004-09-23 2009-12-31 Bayer Cropscience Ag Postopki in sredstva za izdelavo hialuronana
ES2636998T3 (es) * 2004-11-24 2017-10-10 Albumedix A/S Método de reticulación de ácido hialurónico con divinil sulfona
EP1833882A1 (en) * 2004-12-30 2007-09-19 Novozymes Biopolymer A/S Hyaluronic acid linked with a polymer of an alpha hydroxy acid
EP1696032A1 (de) * 2005-02-23 2006-08-30 Bayer CropScience GmbH Verfahren und Mittel zur Herstellung von Hyaluronan in Pilzen
JP4639904B2 (ja) 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
JP4609177B2 (ja) 2005-04-28 2011-01-12 チッソ株式会社 カルシウム結合型発光蛋白質溶液の発光時間延長方法
US20060287512A1 (en) * 2005-05-04 2006-12-21 Novozymes Biopolymer A/S Method of controlling the content of selected component(s) from polymer(s) using molecular sieve(s)
WO2007023682A1 (ja) * 2005-08-25 2007-03-01 Toyo Boseki Kabushiki Kaisha ヒアルロン酸生産植物
EP1772052A1 (de) 2005-10-05 2007-04-11 Bayer CropScience GmbH Verbesserte Verfahren und Mittel zur Herstellung von Hyaluronan
PL1951878T3 (pl) * 2005-10-05 2015-07-31 Bayer Ip Gmbh Rośliny o zwiększonym wytwarzaniu hialuronianu
JP2007174957A (ja) * 2005-12-27 2007-07-12 Toyobo Co Ltd ヒアルロン酸生産酵母
US20080038780A1 (en) * 2006-02-15 2008-02-14 Novozymes Biopolymer A/S Production of low molecular weight hyaluronic acid
DK1999160T3 (da) * 2006-03-14 2010-04-26 Novozymes Biopharma Dk As Acryleret hyaluronsyre
CN101501075B (zh) 2006-08-04 2013-07-10 诺维信生物制药丹麦公司 支化透明质酸和制造方法
DK2089516T3 (da) 2006-11-29 2012-02-20 Novozymes Inc Fremgangsmåder til at forbedre indføringen af DNA i bakterielle celler
US8268586B2 (en) 2006-12-21 2012-09-18 Novozymes, Inc. Modified messenger RNA stabilizing sequences for expressing genes in bacterial cells
WO2009026635A1 (en) * 2007-08-31 2009-03-05 The University Of Queensland Production of hyaluronic acid
EP2031053A1 (en) * 2007-08-31 2009-03-04 The University Of Queensland Production of HA
EP2036983A1 (de) * 2007-09-12 2009-03-18 Bayer CropScience AG Pflanzen, die erhöhte Mengen an Glucosaminglycanen synthetisieren
JP5523338B2 (ja) * 2007-12-19 2014-06-18 エヴォニク ゴールドシュミット ゲーエムベーハー エマルジョン中の架橋ヒアルロン酸
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CN102021213B (zh) * 2009-09-17 2014-07-09 上海佰加壹医药有限公司 一种发酵生产透明质酸发酵液的方法
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IT1401498B1 (it) 2010-07-30 2013-07-26 Mero Srl Idrogelo a base di acido ialuronico e suo uso in ortopedia
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CN102911887A (zh) * 2011-08-02 2013-02-06 山东省生物药物研究院 一种利用毕赤酵母基因调控发酵生产不同分子量透明质酸的方法
CN103781473A (zh) 2011-09-02 2014-05-07 诺维信生物制药丹麦公司 包含用于持续药物释放的透明质酸的口服配制品
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KR101736790B1 (ko) * 2012-02-21 2017-05-17 블루메이지 프레다 바이오팜 컴퍼니 리미티드 바실러스, 히알루론산 효소, 및 그의 용도
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WO2014100837A1 (en) 2012-12-17 2014-06-26 Bui The Duy Computer aided implantation of body implants
EP3074427B1 (en) 2013-11-25 2021-01-06 Deuteria Biomaterials, LLC Deuterium-enriched hyaluronan
CA2896038C (en) * 2015-07-03 2022-08-09 Glycobiosciences Inc. Polymer matrix compositions comprising a high concentration of bio-fermented sodium hyaluronate and uses thereof
CN106755022B (zh) * 2015-11-25 2020-08-04 中国科学院大连化学物理研究所 乙酰葡萄糖胺磷酸变位酶AtAGM编码基因及其酶、制备、应用与酶活性检测方法
EP3481949B1 (en) 2016-07-05 2021-06-09 Novozymes A/S Pectate lyase variants and polynucleotides encoding same
CN107653280B (zh) * 2016-07-25 2021-05-04 安琪酵母股份有限公司 透明质酸发酵用的组合物、培养基和方法及其应用
CN106381279B (zh) * 2016-08-29 2019-11-05 中国药科大学 一种细菌胞外多糖、其制备方法及其应用
WO2020122429A1 (ko) * 2018-12-10 2020-06-18 대화제약 주식회사 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
WO2020122430A1 (ko) * 2018-12-10 2020-06-18 대화제약 주식회사 비병원성 세균을 이용하여 히알루론산 생산을 위한 발현 시스템 및 상기 발현 시스템을 이용한 히알루론산 생산방법
KR102135044B1 (ko) * 2018-12-10 2020-07-17 대화제약 주식회사 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
CN110055201B (zh) * 2019-03-05 2021-05-28 江南大学 一种高产透明质酸寡糖的重组枯草芽孢杆菌的构建方法
RU2719140C1 (ru) 2019-07-26 2020-04-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" Бактерия рода bacillus, продуцирующая гиалуроновую кислоту, и способ получения гиалуроновой кислоты с использованием указанной бактерии
RU2723722C1 (ru) * 2019-07-26 2020-06-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" ТАНДЕМНЫЙ ПРОМОТОР, ФУНКЦИОНИРУЮЩИЙ В БАКТЕРИИ РОДА Bacillus, ТРАНСФОРМИРОВАННАЯ БАКТЕРИЯ РОДА Bacillus - ПРОДУЦЕНТ ЦЕЛЕВОГО ПРОДУКТА, СОДЕРЖАЩАЯ УКАЗАННЫЙ ПРОМОТОР, И СПОСОБ ПОЛУЧЕНИЯ ЦЕЛЕВОГО ПРОДУКТА С ИСПОЛЬЗОВАНИЕМ УКАЗАННОЙ БАКТЕРИИ
CN111518825B (zh) * 2020-04-30 2022-10-11 浙江工业大学 一种多基因组合表达制备蛹虫草多糖的方法
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Also Published As

Publication number Publication date
KR100885163B1 (ko) 2009-02-23
US20140038235A1 (en) 2014-02-06
US8574886B2 (en) 2013-11-05
KR20040085142A (ko) 2004-10-07
CA2471148C (en) 2013-04-09
PL212928B1 (pl) 2012-12-31
EP1572895A2 (en) 2005-09-14
WO2003054163A2 (en) 2003-07-03
CN1636052A (zh) 2005-07-06
PL375190A1 (en) 2005-11-28
BR0215220A (pt) 2007-01-09
DE60239495D1 (de) 2011-04-28
EP1572895B1 (en) 2011-03-16
CN1636052B (zh) 2014-07-02
RU2004122420A (ru) 2005-10-10
JP2005525091A (ja) 2005-08-25
US20120149067A1 (en) 2012-06-14
PL399352A1 (pl) 2012-10-08
CA2803931A1 (en) 2003-07-03
CA2471148A1 (en) 2003-07-03
AU2002366711A1 (en) 2003-07-09
ES2362354T3 (es) 2011-07-04
DK1572895T3 (da) 2011-07-11
HU228693B1 (hu) 2013-05-28
US8137951B2 (en) 2012-03-20
WO2003054163A3 (en) 2005-10-27
IL162302A (en) 2011-01-31
US20110014662A1 (en) 2011-01-20
HUP0700154A2 (en) 2007-05-29
US8093036B2 (en) 2012-01-10
AU2002366711B2 (en) 2007-11-29
ATE502115T1 (de) 2011-04-15
CZ2004765A3 (cs) 2004-12-15
KR100879908B1 (ko) 2009-01-21
HU1300136D0 (hu) 2007-05-29
US7811806B2 (en) 2010-10-12
RU2346049C2 (ru) 2009-02-10
US20030175902A1 (en) 2003-09-18
KR20080018965A (ko) 2008-02-28
EP1572895A4 (en) 2007-07-25
US20110189737A1 (en) 2011-08-04
AU2002366711C1 (en) 2009-01-22

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