HUE028895T2 - Monociták ko-differenciálása és aktiválása allogén donorokból, gyulladáskeltõ dendrites sejtek biztosítására - Google Patents
Monociták ko-differenciálása és aktiválása allogén donorokból, gyulladáskeltõ dendrites sejtek biztosítására Download PDFInfo
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- HUE028895T2 HUE028895T2 HUE15161369A HUE15161369A HUE028895T2 HU E028895 T2 HUE028895 T2 HU E028895T2 HU E15161369 A HUE15161369 A HU E15161369A HU E15161369 A HUE15161369 A HU E15161369A HU E028895 T2 HUE028895 T2 HU E028895T2
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- dcs
- allogeneic
- leukocytes
- activation
- monocytes
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (7)
- Morwclták kö-diffefene!álása és aktiválása átlagén donorokból, gyul^adásksifő döí^d rites sejtek biztosítására Szabadalmi igénypontok1. Epris gyuiiadáskeltŐ dendrites sejtek (DC-k) előállítására, amely eljárás m áláibí lipesekét îaïtaimâMâ: - allogén leukoeiták keverékének előállítása, ameiy ailogén ieukocitákat legalább kát kölőnőpzi aliegén donorbèl szerezzük be, « aíiogén monoeiták izolálása azerolitettaltogén leukocita keverékből, és így roonocita-düsltott aíiogioIeukocitákat áliiunk elé, - nem-kimerült íreden OOk generálása az említett monodta-dúsított ailogén ieukock Iákból, ahol á nem-kimerült^^ ínatíen DÉMk generálását az említett monocíta-dúsitott allogén leukoeiták nem-humán szérumtói mentes vizes sejttenyésztő közegben 2-7 napon át történő egyidejű tenyésztésével valósítjuk meg. ahoi az említett közeget inter1eukin4-ei (IU4) és grarsulodta-makrofág kolónia stimuláló faktorral (GM-GSF) egészítjük ki; és a nem-kimerült éretien DC-k aktiválása, amellyel gyuiíadáskeltö DO-ket kapunk.
- 2. Az 1. igénypont szerinti epres, áhöí áz említett sejttenyésztő közeg legalább égy, a íraszterníi, albumin és inzulin által alkotott csoportból kiválasztott humán poiípeptidef tartalmaz.
- 3. Az 1. vagy 2. igénypont szerinti eljárás, ahoi az említett monocíta-dúsítotí ailogén ieukoclták allogén oeutrofíieket tartalmaznak.
- 4. Az 1 -3. igénypontok bármelyike szerinti eljárás, ahol az említeti ailogén leukocita keverékét legalább két, ieukocitákat tartalmazó buffy-coat összegyűjtésével képezzük, amely burty-eoaböt legalább két különböző ailogén donortól szerezzük be. $> Az 1-3. igénypontok bármelyike szerinti eljárás, ahol az említett aliogén leukocita keveréket az alábbiak szerint iitjuk e!5: - Ieukocitákat eiuálunk legalább két leukoeita ülepítő szűrőről, amely szűrőkéi korábban feukocltáknak a teljes vérből történő Riülepiiésére használtak, ahoi az említett teljes vért legalább két különböző ailogén donorból gyűjtöttük be; és - a kapott ieukocitákat egybegyűjtve előállítjuk az említett ailogén leukocita keveréket; vagy - leukocitákat eluáiunk egy leukocita ülepítő szűrőről, amely szűrőt korábban ieukoci- tiknak összegyűjtött buffy-coat-bPi történő kiülapítésér© használtak, ahol m említeti összegyűjtött bu%-őöát~öt legalább két különböző aliogén donorból gyűjtöttük be.
- 8. Az előző igénypontok bármelyike szerinti eljárás, ahol az említett aliogén monobltikst ülápltéssel vagy antitest/győngy izolálással állítjuk elő % Az előző igénypontok bármelyike szerinti eljárás* ahol az említett együttes tenyésztést körülbelül f napig végezzük.8. Az előző Igénypontok bármelyike szerinti eljárás, amelynél további lépésként a nem^imerüít éretlen Decket egy antigénnel töltjük be.
- 9, Az előző igénypöhtök bármelyik© szerinti eljárás, amelynél az aktiválást a Toll-hasonlatos receptor 3 (TLR3}-lígandum PclM:C. egy TLR?/8-ligandum - a TLRT/8-ligandu-mot a Ressquímod, Gardíquimod és az Imlquimod által aíkotott csoportból választjuk ki — és ez aktiválás índukáiásáre, interferon gamma (SFN~y) hozzáadásával valósítjuk meg. 18, A 9. igénypont szerinti eljárás, amelyben ez aktiválás továbbá magában foglalja legalébb ®gy. á TlRé-iigandumok által alkotott csoportbói kiválasztott anyagnak a hozzáadását, amelyeket a llpopofiszaoháríd és monofoszforil iípid A, olyan CpG oligonukleotíd (ÖDN) szekvenciák amelyek megkülönböztetik a mikrebiáíís PNS-t az emlős DNS-föl, interferon alfa (iFN-α), interleukin Iß (IL-Iß) és a tumor nekrézis faktor alfa (TNF-α) által alkotott ösöpbrthé! vilaszíünk ki. il A 9. vagy 10, igénypont szeonf après, amelyben az említett aktiválás nem foglap magában prosztaglandin E2 (PGE2) hozzáadását. 12. A 9-11. igénypontok bármelyike szerinti eljárás, amelyben az éretlen DC-ket az aktivációs faktoroknak 8-24 órán keresztül tesszük ki, ezt kővetően pedig lényegileg minden aktivációs faktort elmosunk,
- 13. Gyógyszerkészítmény, amely tartalmazza legalább kit egymástól eltérő allogên donortól származó, aliogén gyulladáskeltő dendrites sejtek keverékét, amely dendrites sejtek az 1-12. igénypontok bármelyike ázéhnti^eljárássaiállíthatók elő, valamint legalább agy gyógyszerészeiileg elfogadható hordozót. 14. A 13. igényperé szerinti készítmény, a rák kezelésében valő alkalmazásra.
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| HUE13814106A HUE027619T2 (hu) | 2012-12-18 | 2013-12-18 | Monociták ko-differenciálása allogén donorokból |
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| PL3460052T3 (pl) | 2017-09-20 | 2020-05-18 | Immunicum Ab | Ulepszone allogeniczne komórki dendrytyczne do stosowania w leczeniu nowotworu |
| CA3087418A1 (en) * | 2018-01-18 | 2019-07-25 | University Of South Florida | Dead antigen stimulated immature heterogenous dendritic cells as therapeutics for diseases |
| CN108660125A (zh) * | 2018-05-14 | 2018-10-16 | 武汉博百欧生物科技有限公司 | 一种从人体输血废弃物中提取中性粒细胞嗜天青颗粒的方法 |
| WO2020154424A1 (en) * | 2019-01-22 | 2020-07-30 | The Brigham And Women's Hospital, Inc. | Antigen-presenting neutrophil-derived dendritic cells and methods of use thereof |
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| WO2001051617A1 (en) * | 2000-01-11 | 2001-07-19 | Maxygen, Inc. | Monocyte-derived dendritic cell subsets |
| AU2002326463A1 (en) | 2001-07-25 | 2003-02-17 | Northwest Biotherapeutics, Inc. | Methods and apparatus for enrichment and culture of monocytic dendritic cell precursors |
| SI1509244T1 (sl) | 2002-06-06 | 2011-11-30 | Immunicum An | Nov postopek in spojina za izdelavo celične alogenične vakcine |
| KR100585456B1 (ko) * | 2003-09-30 | 2006-06-07 | 국립암센터 | 동종 종양-관련 항원이 탑재된 수지상세포, 이의 제조방법및 이를 유효성분으로 하는 암 치료용 백신 조성물 |
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| EP1806395A1 (en) * | 2006-01-06 | 2007-07-11 | Stichting Sanquin Bloedvoorziening | Maturation of dendritic cells |
| EP1840206A1 (en) * | 2006-03-28 | 2007-10-03 | Gsf-Forschungszentrum Für Umwelt Und Gesundheit, Gmbh | Compositions for the preparation of mature dendritic cells |
| EP2494038B1 (en) * | 2009-10-27 | 2019-06-26 | Immunicum AB | Method for proliferation of antigen-specific t cells |
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