HUE027619T2 - Monociták ko-differenciálása allogén donorokból - Google Patents
Monociták ko-differenciálása allogén donorokból Download PDFInfo
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- HUE027619T2 HUE027619T2 HUE13814106A HUE13814106A HUE027619T2 HU E027619 T2 HUE027619 T2 HU E027619T2 HU E13814106 A HUE13814106 A HU E13814106A HU E13814106 A HUE13814106 A HU E13814106A HU E027619 T2 HUE027619 T2 HU E027619T2
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- dcs
- allogeneic
- leukocytes
- monocytes
- donors
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Claims (8)
- Mormolták ko-dltterencsálása aliogén donorokból Szabadalmi igénypontok 1. E|árés nem-kimerűlt éretlen dendrites séfek (DC-k) előállítására, amely éliras az alábbi lépéseket tartalmazza: - aliogén leukoeiták keverékét állítjuk elő, amely ailogén íeukociíákat legalább két különböző aliogén donorból szerezzük be, - az említett aifogén leukoclta keverékből Izoláljuk az aliogén roonocstákat és így monoeita-dúsított aliogén leukoeitákat állítunk ele, és * az említett monoGlta-dúsított aliogén ieuköciiákból nem-kimerült éretlen DOket állítunk elő, almi a nem kimerni éretlen dendrites sejtek {DC-k) előállítását az említett mrmoeiía-dúsioi aliogén leukocíták nem-bumán szérumtól mentes vizes sejttenyész-tö közegben 2-7 napon át történd egyidejű tenyésztésével végezzük, ahol az említett közeget ínterieukín4~eí (IL-4) és granulocíta-makrofág kolónia stimuláló faktorral (GM-CSF) egészítjük ki.
- 2. Az 1. Igénypont szerlbtiepris, ahol az említett sejttenyészíő közeg legalább egy a traszfemn, albumin és inzulin közül választott humán polipeptídef tartalmaz.
- 3. Az 1, vagy 2, igénypont szerinti eljárás* ahol az említett monocita-dúsitott aliogén leukoeiták aliogén nautröieket tartalmaznak,
- 4. Az 1-3. igénypontok bármelyike szerinti eljárás, ahol az említeti aliogén; ietiko-clta keveréket legalább két, leukoeitákat tartalmazó buífy-coat összegyűjtésével képezzük, amely büffy-ooaí-et legalább két különböző donortól szerezzük be.
- 5. Az 1-3, igénypontok bármelyike szerinti eljárási, aboi az említett ailogén leuko-cita keveréket az alábbiak szerint állítjuk elő: - leukoeitákat; eluáiunk iegalébb két leukoela ülepítő szűréről, amely szőröket korábban leukodláknak a teles vérből történő kíüiepítésére használtak, ahol az említet tek jós vért legalább két különböző aliogén donorból gyűjtöttük bej és - a kapót leukoeitákat egybegyűjtve előállítjuk az említett aliogén leukoclta keveréket;; vagy - leukocitákat efuâiunk egy ieokocka ülepítő szűrőről amely szűrőt korábban leukocl-táknak összegyűjtött feyfy-coaí-bol történő ktűtsptésére használtak, ah©!; az említett összegyűjtött butfy-coai-ot legalább két különböző allogén donorból gyűjtöttük be.
- 6. Az előző Igénypontok bármelyike szerinti eljárás, ahol az említett allogén monocitákat ülepítéssei vagy antitest/gyöngy Izolálással állítjuk elő
- 7. Az előző Igénypontok bármelyike szerinti eljárás, ahol az említett együttes tenyésztést kb. 5 napig végezzük.
- 8. Az előző Igénypontok bármelyike szerinti eljárás, amelynél további lépésként a nem-kimerült éretlen DC-ket egy antigénnel töltjük be.
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| HUE13814106A HUE027619T2 (hu) | 2012-12-18 | 2013-12-18 | Monociták ko-differenciálása allogén donorokból |
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| HUE15161369A HUE028895T2 (hu) | 2012-12-18 | 2013-12-18 | Monociták ko-differenciálása és aktiválása allogén donorokból, gyulladáskeltõ dendrites sejtek biztosítására |
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| EP (2) | EP2788474B1 (hu) |
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| WO (1) | WO2014096033A1 (hu) |
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| PL3460052T3 (pl) | 2017-09-20 | 2020-05-18 | Immunicum Ab | Ulepszone allogeniczne komórki dendrytyczne do stosowania w leczeniu nowotworu |
| CA3087418A1 (en) * | 2018-01-18 | 2019-07-25 | University Of South Florida | Dead antigen stimulated immature heterogenous dendritic cells as therapeutics for diseases |
| CN108660125A (zh) * | 2018-05-14 | 2018-10-16 | 武汉博百欧生物科技有限公司 | 一种从人体输血废弃物中提取中性粒细胞嗜天青颗粒的方法 |
| US20220106566A1 (en) * | 2019-01-22 | 2022-04-07 | The Brigham And Women`S Hospital, Inc. | Antigen-Presenting Neutrophil-Derived Dendritic Cells and Methods of Use Thereof |
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| EP1259592A1 (en) * | 2000-01-11 | 2002-11-27 | Maxygen, Inc. | Monocyte-derived dendritic cell subsets |
| WO2003010292A2 (en) * | 2001-07-25 | 2003-02-06 | Northwest Biotherapeutics, Inc. | Methods and apparatus for enrichment and culture of monocytic dendritic cell precursors |
| WO2003103707A1 (en) | 2002-06-06 | 2003-12-18 | Immunicum Ab | New method and composition for producing a cellular allogeneic vaccine |
| KR100585456B1 (ko) * | 2003-09-30 | 2006-06-07 | 국립암센터 | 동종 종양-관련 항원이 탑재된 수지상세포, 이의 제조방법및 이를 유효성분으로 하는 암 치료용 백신 조성물 |
| CA2504451A1 (en) * | 2004-08-10 | 2006-02-10 | Geron Corporation | Dendritic cell vaccines for treating cancer made from embryonic stem cells |
| WO2007067782A2 (en) * | 2005-12-08 | 2007-06-14 | Northwest Biotherapeutics, Inc. | Compositions and methods for inducing the activation of immature monocytic dendritic cells |
| EP1806395A1 (en) * | 2006-01-06 | 2007-07-11 | Stichting Sanquin Bloedvoorziening | Maturation of dendritic cells |
| EP1840206A1 (en) * | 2006-03-28 | 2007-10-03 | Gsf-Forschungszentrum Für Umwelt Und Gesundheit, Gmbh | Compositions for the preparation of mature dendritic cells |
| EP2494038B1 (en) * | 2009-10-27 | 2019-06-26 | Immunicum AB | Method for proliferation of antigen-specific t cells |
| DK2534242T3 (en) * | 2010-02-10 | 2016-07-18 | Immunicum Ab | Improved composition for the inhibition of tumor cell proliferation |
| US9492529B2 (en) * | 2011-04-13 | 2016-11-15 | Immunicum Ab | Method for priming of T cells |
| JP2014514927A (ja) * | 2011-04-13 | 2014-06-26 | イミュニカム・エイビイ | 抗原特異的ｔ細胞の増殖のための方法 |
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