HK1089485A1 - Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process - Google Patents

Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process

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Publication number
HK1089485A1
HK1089485A1 HK06106593.2A HK06106593A HK1089485A1 HK 1089485 A1 HK1089485 A1 HK 1089485A1 HK 06106593 A HK06106593 A HK 06106593A HK 1089485 A1 HK1089485 A1 HK 1089485A1
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HK
Hong Kong
Prior art keywords
whole
amplification
analysis
polymerization process
libraries generated
Prior art date
Application number
HK06106593.2A
Other languages
English (en)
Inventor
Emmanuel Kamberov
Tong Sun
Eric Bruening
Jon Pinter
Irina Sleptsova
Takao Kurihara
Vladimir L Makarov
Original Assignee
Rubicon Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rubicon Genomics Inc filed Critical Rubicon Genomics Inc
Publication of HK1089485A1 publication Critical patent/HK1089485A1/xx

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK06106593.2A 2003-03-07 2006-06-08 Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process HK1089485A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45306003P 2003-03-07 2003-03-07
PCT/US2004/006983 WO2004081225A2 (en) 2003-03-07 2004-03-08 Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process

Publications (1)

Publication Number Publication Date
HK1089485A1 true HK1089485A1 (en) 2006-12-01

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Family Applications (1)

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HK06106593.2A HK1089485A1 (en) 2003-03-07 2006-06-08 Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process

Country Status (8)

Country Link
US (2) US7718403B2 (xx)
EP (2) EP1604040B1 (xx)
JP (1) JP4773338B2 (xx)
AT (1) ATE484598T1 (xx)
DE (1) DE602004029560D1 (xx)
DK (2) DK2374900T3 (xx)
HK (1) HK1089485A1 (xx)
WO (1) WO2004081225A2 (xx)

Families Citing this family (147)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040024193A1 (en) * 2002-07-26 2004-02-05 Williams Gregg T. Method of detecting and quantifying hepatitis C virus
US20060183132A1 (en) * 2005-02-14 2006-08-17 Perlegen Sciences, Inc. Selection probe amplification
US20090124514A1 (en) * 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US7670810B2 (en) 2003-06-20 2010-03-02 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US7459274B2 (en) * 2004-03-02 2008-12-02 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
JP3972106B2 (ja) * 2004-03-03 2007-09-05 大学共同利用機関法人情報・システム研究機構 ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー
CA2902980A1 (en) * 2004-03-08 2005-09-29 Rubicon Genomics, Inc. Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation
PT1794327T (pt) * 2004-09-14 2017-01-24 Argos Therapeutics Inc Amplificação independente de estirpes de patogénios e vacinas para os mesmos
AU2005322131B2 (en) * 2004-12-23 2011-11-10 Global Life Sciences Solutions Usa Llc Ligation-based RNA amplification
JP2008529538A (ja) * 2005-02-16 2008-08-07 ジェネティック テクノロジーズ リミテッド 相補性デュプリコンの増幅を含む遺伝子分析方法
US7449297B2 (en) * 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
DK2463386T3 (en) 2005-06-15 2017-07-31 Complete Genomics Inc Nucleic acid analysis using random mixtures of non-overlapping fragments
US20070003938A1 (en) * 2005-06-30 2007-01-04 Perlegen Sciences, Inc. Hybridization of genomic nucleic acid without complexity reduction
EP1924705A1 (en) * 2005-08-02 2008-05-28 Rubicon Genomics, Inc. Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
US20090137415A1 (en) * 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
CA2620081A1 (en) * 2005-09-16 2007-03-29 454 Life Sciences Corporation Cdna library preparation
JP5457673B2 (ja) * 2005-09-20 2014-04-02 ベリデックス・リミテッド・ライアビリティ・カンパニー ユニーク配列のdnaプローブを作製するための方法および組成物、dnaプローブの標識、ならびにこれらプローブの使用
US9134237B2 (en) * 2005-09-20 2015-09-15 Janssen Diagnotics, LLC High sensitivity multiparameter method for rare event analysis in a biological sample
US20070092899A1 (en) * 2005-10-21 2007-04-26 The Regents Of The University Of California Multiple displacement amplification with blocker DNA
CA2629008A1 (en) 2005-11-08 2007-09-13 Euclid Diagnostics Llc Materials and methods for assaying for methylation of cpg islands associated with genes in the evaluation of cancer
US20080057499A1 (en) * 2006-02-06 2008-03-06 Affymetrix, Inc. Methods for high specificity whole genome amplification and hybridization
EP2002016B1 (en) * 2006-04-12 2009-11-18 Medical Research Council Method for determining copy number
WO2007136874A2 (en) * 2006-05-18 2007-11-29 President And Fellows Of Harvard College Genomic library construction
US20080026390A1 (en) * 2006-06-14 2008-01-31 Roland Stoughton Diagnosis of Fetal Abnormalities by Comparative Genomic Hybridization Analysis
EP3159417B1 (en) * 2006-08-01 2021-10-06 Gen-Probe Incorporated Methods of nonspecific target capture of nucleic acids
US9149473B2 (en) * 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
ES2582602T3 (es) 2007-03-28 2016-09-14 Signal Diagnostics Sistema y método de análisis de alta resolución de ácidos nucleicos para detectar variaciones de secuencia
US7794985B2 (en) * 2007-04-04 2010-09-14 Ghc Technologies, Inc. Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins
EP2164985A4 (en) * 2007-06-01 2014-05-14 454 Life Sciences Corp SYSTEM AND METHOD FOR IDENTIFYING INDIVIDUAL SAMPLES FROM A MULTIPLEX MIXTURE
WO2009017743A2 (en) * 2007-07-30 2009-02-05 Argos Therapeutics, Inc. Improved primers and probes for the amplification and detection of hiv gag, rev and nef polynucleotides
EP2198042B1 (en) 2007-09-17 2016-11-02 MDxHealth SA Novel markers for bladder cancer detection
US20090099040A1 (en) * 2007-10-15 2009-04-16 Sigma Aldrich Company Degenerate oligonucleotides and their uses
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US20090291475A1 (en) * 2008-04-23 2009-11-26 Kai Qin Lao Sequence amplification with linear primers
US20100120097A1 (en) * 2008-05-30 2010-05-13 Board Of Regents, The University Of Texas System Methods and compositions for nucleic acid sequencing
US20100028955A1 (en) * 2008-07-22 2010-02-04 Life Technologies Corporation Sequence Amplification with Target Primers
JP5652843B2 (ja) * 2008-10-17 2015-01-14 独立行政法人農業・食品産業技術総合研究機構 Dna増幅法
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
WO2010080691A1 (en) * 2009-01-06 2010-07-15 Qimin You Cross priming amplification of target nucleic acids
US9388471B2 (en) 2009-03-13 2016-07-12 Mdxhealth Sa Methylation of the GATA4 gene in urine samples as a marker for bladder cancer detection
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
FR2949120A1 (fr) * 2009-08-13 2011-02-18 Centre Nat Rech Scient Procede de detection d'un adn circularise et utilisation de ce procede pour la detection de mutations
WO2011044437A2 (en) 2009-10-09 2011-04-14 Stc.Unm Polony sequencing methods
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US20110195457A1 (en) * 2010-02-09 2011-08-11 General Electric Company Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
DK201000356A (en) * 2010-04-22 2011-10-23 Statens Seruminstitut New nucleotide sequence amplification Method
KR101334072B1 (ko) * 2010-11-25 2013-11-29 한국표준과학연구원 핵산 정량 방법 및 키트
US9738930B2 (en) 2011-01-28 2017-08-22 The Broad Institute, Inc. Paired end bead amplification and high throughput sequencing
WO2012113780A1 (en) 2011-02-21 2012-08-30 Qiagen Gmbh Method for quantifying human dna
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2012166425A2 (en) * 2011-05-27 2012-12-06 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
WO2013022961A1 (en) * 2011-08-08 2013-02-14 3The Broad Institute Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
ES2663234T3 (es) 2012-02-27 2018-04-11 Cellular Research, Inc Composiciones y kits para recuento molecular
SG11201405669XA (en) 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
WO2013143133A1 (zh) * 2012-03-30 2013-10-03 深圳华大基因科技服务有限公司 全基因组扩增方法及其应用
WO2013164319A1 (en) * 2012-04-30 2013-11-07 Qiagen Gmbh Targeted dna enrichment and sequencing
EP4071253A1 (en) * 2012-07-26 2022-10-12 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
CN104797718B (zh) * 2012-09-12 2020-05-08 加利福尼亚大学董事会 通过单链扩增和测序对单个细胞进行精确基因组测序
EP2722399A1 (en) * 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification
KR101451874B1 (ko) * 2013-02-27 2014-10-16 국민대학교산학협력단 실시간 pcr을 이용한 미생물의 최단시간 검출방법
EP3013984B1 (en) 2013-06-25 2023-03-22 Prognosys Biosciences, Inc. Methods for determining spatial patterns of biological targets in a sample
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
US10370707B2 (en) 2013-10-09 2019-08-06 Fluoresentric, Inc. Multiplex probes
CN105917002A (zh) 2013-11-18 2016-08-31 鲁比康基因组学公司 用于减少背景的可降解适体
US9293316B2 (en) * 2014-04-04 2016-03-22 Thermo Finnigan Llc Ion separation and storage system
CN105463585B (zh) 2014-09-12 2018-04-20 清华大学 基于单链dna分子构建测序文库的方法及其应用
EP3208343B1 (en) * 2014-10-13 2022-01-05 MGI Tech Co., Ltd. Nucleic acid fragmentation method and sequence combination
WO2016058173A1 (zh) * 2014-10-17 2016-04-21 深圳华大基因研究院 一种用于核酸随机片段化的引物及核酸随机片段化方法
US10968536B2 (en) 2015-02-25 2021-04-06 Jumpcode Genomics, Inc. Methods and compositions for sequencing
US9727810B2 (en) 2015-02-27 2017-08-08 Cellular Research, Inc. Spatially addressable molecular barcoding
CN107406888A (zh) 2015-03-30 2017-11-28 赛卢拉研究公司 用于组合条形编码的方法和组合物
WO2016160965A1 (en) 2015-03-30 2016-10-06 Rubicon Genomics, Inc. Methods and compositions for repair of dna ends by multiple enzymatic activities
EP3277837A1 (en) * 2015-03-31 2018-02-07 Qiagen GmbH Efficiency improving ligation methods
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
ES2961374T3 (es) * 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
US10894980B2 (en) 2015-07-17 2021-01-19 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
CN107922970B (zh) * 2015-08-06 2021-09-28 豪夫迈·罗氏有限公司 通过单探针引物延伸的靶标富集
CN105602939A (zh) * 2015-09-02 2016-05-25 序康医疗科技(苏州)有限公司 扩增dna的方法
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ, インコーポレイテッド ライブラリー正規化のための方法および組成物
CN105506125B (zh) * 2016-01-12 2019-01-22 上海美吉生物医药科技有限公司 一种dna的测序方法及一种二代测序文库
US11339427B2 (en) 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
WO2018003220A1 (ja) * 2016-06-29 2018-01-04 トヨタ自動車株式会社 Dnaライブラリーの作製方法及びdnaライブラリーを用いたゲノムdna解析方法
JP7343264B2 (ja) * 2016-06-29 2023-09-12 トヨタ自動車株式会社 Dnaライブラリーの作製方法及びdnaライブラリーを用いたゲノムdna解析方法
JP6889769B2 (ja) * 2016-07-18 2021-06-18 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 核酸配列決定の非対称な鋳型および非対称な方法
CA3034959A1 (en) * 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of whole genome digital amplification
CN110012671B (zh) * 2016-09-06 2024-05-28 合成Dna技术公司 Ngs文库浓度的标准化
KR102522023B1 (ko) 2016-09-26 2023-04-17 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
WO2018061674A1 (ja) 2016-09-30 2018-04-05 富士フイルム株式会社 脊椎動物由来の単一細胞の塩基配列情報を取得する方法
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2019018561A1 (en) * 2017-07-19 2019-01-24 The Scripps Research Institute GENOMIC LIBRARY GENERATION IN SOLID PHASE FOR HIGH FLOW SEQUENCING
EP3665297A4 (en) 2017-08-11 2021-05-12 Atila Biosystems Incorporated DIGITAL AMPLIFICATION WITH LIMITED NUCLEOTIDIC COMPOSITION PRIMERS
WO2019113457A1 (en) * 2017-12-07 2019-06-13 Massachusetts Institute Of Technology Single cell analyses
CN109971843B (zh) * 2017-12-27 2022-11-11 复旦大学泰州健康科学研究院 一种单细胞转录组的测序方法
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CN112805389A (zh) 2018-10-01 2021-05-14 贝克顿迪金森公司 确定5’转录物序列
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
SG11202105824RA (en) 2018-12-10 2021-06-29 10X Genomics Inc Imaging system hardware
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN113574178A (zh) 2019-01-23 2021-10-29 贝克顿迪金森公司 与抗体关联的寡核苷酸
CN113166757A (zh) * 2019-02-15 2021-07-23 宝生物工程(美国)有限公司 核酸文库的制备和分析方法
WO2020191521A1 (zh) * 2019-03-22 2020-10-01 深圳华大智造科技有限公司 核酸序列、rna目标区域测序文库的构建方法及应用
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP3994262A4 (en) * 2019-06-26 2023-09-20 Stratos Genomics Inc. IMPROVEMENT OF NUCLEIC ACID POLYMERIZATION THROUGH AROMATIC COMPOUNDS
CN114051534A (zh) 2019-07-22 2022-02-15 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
GB201911515D0 (en) * 2019-08-12 2019-09-25 Univ London Queen Mary Methods for generating a population of polynucleotide molecules
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021127406A1 (en) * 2019-12-19 2021-06-24 The Regents Of The University Of California Methods of producing target capture nucleic acids
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11702689B2 (en) * 2020-04-24 2023-07-18 Microsoft Technology Licensing, Llc Homopolymer primers for amplification of polynucleotides created by enzymatic synthesis
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
EP4153776A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US11280028B1 (en) 2021-02-24 2022-03-22 Agency For Science, Technology And Research (A*Star) Unbiased and simultaneous amplification method for preparing a double-stranded DNA library from a sample of more than one type of nucleic acid
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023086880A1 (en) * 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
DE102022211087A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
AU622104B2 (en) 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
DE68908054T2 (de) 1988-01-21 1994-03-10 Genentech Inc Verstärkung und nachweis von nukleinsäuresequenzen.
US6107023A (en) 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
AU632760B2 (en) 1988-07-26 1993-01-14 Genelabs Technologies, Inc. Rna and dna amplification techniques
US4932207A (en) 1988-12-28 1990-06-12 Sundstrand Corporation Segmented seal plate for a turbine engine
US5106727A (en) 1989-04-27 1992-04-21 Life Technologies, Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
KR100321510B1 (ko) 1991-09-24 2005-01-10 키진 엔.브이. 선택적인 제한단편의 증폭:디엔에이(dna) 핑거프린트법
WO1993014217A1 (en) * 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
WO1993022461A1 (en) * 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
GB9422891D0 (en) 1994-11-12 1995-01-04 Univ Nottingham Improvements in and relating to the amplification and identification of nucleotide sequences
JPH08173164A (ja) 1994-12-22 1996-07-09 Hitachi Ltd Dna調製法
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
US5994058A (en) 1995-03-20 1999-11-30 Genome International Corporation Method for contiguous genome sequencing
US5871820A (en) 1995-04-06 1999-02-16 General Electric Company Protection of thermal barrier coating with an impermeable barrier coating
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5814444A (en) 1995-06-07 1998-09-29 University Of Washington Methods for making and using single-chromosome amplfication libraries
WO1998004689A1 (en) 1995-07-31 1998-02-05 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US5631147A (en) * 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
EP0880598A4 (en) * 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
EP0880537B1 (en) 1996-02-14 2004-12-15 bioMerieux B.V. Isolation of single stranded nucleic acids
WO1997041261A1 (en) * 1996-05-02 1997-11-06 Immunological Associates Of Denver Quantitation of rna transcripts using genomic dna as the internal amplification competitor
EP0854935B1 (en) 1996-07-16 2005-04-13 Periannan Senapathy Method for contiguous genome sequencing
EP0835935A1 (en) * 1996-10-03 1998-04-15 Roche Diagnostics GmbH Thermostable DNA Polymerase from Anaerocellum thermophilum
US6030814A (en) * 1997-04-21 2000-02-29 Epicentre Technologies Corporation Reverse transcription method
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US5932451A (en) 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
DE19813317A1 (de) 1998-03-26 1999-09-30 Roche Diagnostics Gmbh Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR
US6232067B1 (en) * 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
ES2172353T3 (es) 1998-09-18 2002-09-16 Micromet Ag Amplificacion del adn de una unica celula.
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6521428B1 (en) 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
US6491752B1 (en) 1999-07-16 2002-12-10 Sumco Oregon Corporation Enhanced n-type silicon material for epitaxial wafer substrate and method of making same
WO2001009384A2 (en) 1999-07-29 2001-02-08 Genzyme Corporation Serial analysis of genetic alterations
US6846626B1 (en) * 1999-09-01 2005-01-25 Genome Technologies, Llc Method for amplifying sequences from unknown DNA
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US6271002B1 (en) 1999-10-04 2001-08-07 Rosetta Inpharmatics, Inc. RNA amplification method
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
AU2001232485A1 (en) * 2000-01-13 2001-07-24 Amsterdam Support Diagnostics B.V. A universal nucleic acid amplification system for nucleic acids in a sample
US6379932B1 (en) 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
US6713257B2 (en) * 2000-08-25 2004-03-30 Rosetta Inpharmatics Llc Gene discovery using microarrays
AU2001290639A1 (en) * 2000-09-06 2002-03-22 Rutgers, The State University A method for sequencing nucleic acid sequences without chain termination
AU2002213882A1 (en) 2000-09-08 2002-03-22 Biomerieux B.V.A. Attenuated hiv strains and use thereof
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
WO2002061140A2 (en) * 2001-01-31 2002-08-08 Ambion, Inc. Competitive population normalization for comparative analysis of nucleic acid samples
JP2004527236A (ja) 2001-02-14 2004-09-09 ベイラー カレッジ オブ メディスン Rna増幅の方法及び組成物
DE60220025T2 (de) 2001-03-09 2008-01-17 Nugen Technologies, Inc., San Carlos Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
WO2002103054A1 (en) 2001-05-02 2002-12-27 Rubicon Genomics Inc. Genome walking by selective amplification of nick-translate dna library and amplification from complex mixtures of templates
JP2002345466A (ja) 2001-05-08 2002-12-03 Takara Bio Inc Dnaの増幅方法
US6638722B2 (en) 2001-06-13 2003-10-28 Invitrogen Corporation Method for rapid amplification of DNA
EP1275738A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Method for random cDNA synthesis and amplification
EP1275734A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Method for random cDNA synthesis and amplification
WO2003016546A1 (en) 2001-08-21 2003-02-27 Flinders Technologies Pty Ltd. Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genome mixtures
US20040019005A1 (en) * 2001-10-01 2004-01-29 Jeffrey Van Ness Methods for parallel measurement of genetic variations
JP2005535283A (ja) 2001-11-13 2005-11-24 ルビコン ゲノミクス インコーポレイテッド ランダムフラグメント化により生成されたdna分子を用いたdna増幅および配列決定
US20030211528A1 (en) * 2002-03-12 2003-11-13 Norman Iscove Exponential amplification of sub-picogram nucleic acid samples with retention of quantitative representation
KR100545295B1 (ko) * 2003-09-29 2006-01-24 현대모비스 주식회사 차량용 조향장치의 너클 어셈블리
CA2902980A1 (en) 2004-03-08 2005-09-29 Rubicon Genomics, Inc. Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
FR2957821B1 (fr) 2010-03-24 2014-08-29 Inst Francais Du Petrole Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives

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ATE484598T1 (de) 2010-10-15
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WO2004081225A2 (en) 2004-09-23
DK1604040T3 (da) 2011-01-24
US7718403B2 (en) 2010-05-18
DE602004029560D1 (de) 2010-11-25
EP2374900A1 (en) 2011-10-12
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