ES8802159A1 - Un procedimiento de producir un acido desoxirribonucleico (adn)que tiene o contiene una secuencia de base que codificaun polipeptido de factor de necrosis tumoral de humano - Google Patents

Un procedimiento de producir un acido desoxirribonucleico (adn)que tiene o contiene una secuencia de base que codificaun polipeptido de factor de necrosis tumoral de humano

Info

Publication number
ES8802159A1
ES8802159A1 ES540939A ES540939A ES8802159A1 ES 8802159 A1 ES8802159 A1 ES 8802159A1 ES 540939 A ES540939 A ES 540939A ES 540939 A ES540939 A ES 540939A ES 8802159 A1 ES8802159 A1 ES 8802159A1
Authority
ES
Spain
Prior art keywords
necrosis factor
tumor necrosis
human tumor
dna encoding
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES540939A
Other languages
English (en)
Other versions
ES540939A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dainippon Pharmaceutical Co Ltd
Original Assignee
Dainippon Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP59043617A external-priority patent/JPS60185799A/ja
Priority claimed from JP59082653A external-priority patent/JPS60232097A/ja
Priority claimed from JP59172307A external-priority patent/JP2584206B2/ja
Application filed by Dainippon Pharmaceutical Co Ltd filed Critical Dainippon Pharmaceutical Co Ltd
Publication of ES540939A0 publication Critical patent/ES540939A0/es
Publication of ES8802159A1 publication Critical patent/ES8802159A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

UN ADN QUE CONTIENE UNA SECUENCIA DE BASES CORRESPONDIENTE A OTRA DE AMINOACIDOS (I), SIENDO A UN POLIPEPTIDO (II); QUE CODIFICA UN FACTOR DE NECROSIS DE TUMOR HUMANO, SE PRODUCE POR LAS ETAPAS SIGUIENTES: 1) CULTIVAR MACROFAGOS HUMANOS JUNTO CON INDUCTORES COMO ENDOTOXINA O ESTER DE FORBOL EN CICLOHEXAMIDA; 2) SEPARAR DE ESTOS, POR CROMATOGRAFIA DE AFINIDAD EN OLIGO (DT)-CELULOSA, LA FRACCION CON ARN; 3) PREPARAR A PARTIR DEL MARN UN CADN CON TRANSCRIPTORA INVERSA Y DOBLAR LA CADENA; 4) INSERTAR EL CADN EN UN VECTOR COMO EL PLASMIDO PBR322; 5) INTRODUCIR EL VECTOR RECOMBINANTE EN ESCHERICHIA COLI; 6) CLONAR EL CADN QUE CODIFICA EL FACTOR DE NECROSIS, EMPLEANDO COMO SONDA UN FRAGMENTO DE ADN QUE LO CODIFICA. PRESENTAN UN EFECTO CITOTOXICO SOBRE CELULAS TUMORALES Y PRODUCEN LA REGRESION DE ESTOS EN LOS ANIMALES.
ES540939A 1984-03-06 1985-03-05 Un procedimiento de producir un acido desoxirribonucleico (adn)que tiene o contiene una secuencia de base que codificaun polipeptido de factor de necrosis tumoral de humano Expired ES8802159A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP59043617A JPS60185799A (ja) 1984-03-06 1984-03-06 ヒト癌壊死因子
JP59082653A JPS60232097A (ja) 1984-04-23 1984-04-23 ヒト癌壊死因子ポリペプチドの製造法
JP59172307A JP2584206B2 (ja) 1984-08-17 1984-08-17 ヒト癌壊死因子

Publications (2)

Publication Number Publication Date
ES540939A0 ES540939A0 (es) 1988-04-01
ES8802159A1 true ES8802159A1 (es) 1988-04-01

Family

ID=27291610

Family Applications (2)

Application Number Title Priority Date Filing Date
ES540939A Expired ES8802159A1 (es) 1984-03-06 1985-03-05 Un procedimiento de producir un acido desoxirribonucleico (adn)que tiene o contiene una secuencia de base que codificaun polipeptido de factor de necrosis tumoral de humano
ES550939A Expired ES8703490A1 (es) 1984-03-06 1986-01-16 Un procedimiento para producir un polipeptido que tiene o contiene un polipeptido de factor de necrosis de tumor humano

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES550939A Expired ES8703490A1 (es) 1984-03-06 1986-01-16 Un procedimiento para producir un polipeptido que tiene o contiene un polipeptido de factor de necrosis de tumor humano

Country Status (11)

Country Link
EP (1) EP0155549B1 (es)
KR (1) KR920010225B1 (es)
AU (1) AU584608B2 (es)
CA (1) CA1341240C (es)
DE (2) DE3582183D1 (es)
DK (1) DK172013B1 (es)
ES (2) ES8802159A1 (es)
FR (1) FR99C0037I2 (es)
HU (1) HU202921B (es)
NL (1) NL990034I2 (es)
PH (1) PH22108A (es)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60112718A (ja) * 1983-11-21 1985-06-19 Kyorin Pharmaceut Co Ltd 抗腫瘍作用を示す蛋白性物質及びその製造方法
JPH0695939B2 (ja) * 1983-12-02 1994-11-30 大日本製薬株式会社 ウサギ癌壊死因子をコ−ドするクロ−ン化ｄνａ
US4879226A (en) * 1984-04-06 1989-11-07 Asahi Kasei Kogyo Kabushiki Kaisha Novel human physiologically active polypeptide
GR851626B (es) * 1984-07-05 1985-11-26 Genentech Inc
US6686455B1 (en) 1984-07-05 2004-02-03 Genentech, Inc. Tumor necrosis factor
US5672347A (en) * 1984-07-05 1997-09-30 Genentech, Inc. Tumor necrosis factor antagonists and their use
DE3581730D1 (de) * 1984-10-15 1991-03-14 Cetus Corp Menschlicher tumornekrosisfaktor.
US4959314A (en) 1984-11-09 1990-09-25 Cetus Corporation Cysteine-depleted muteins of biologically active proteins
JP2557053B2 (ja) * 1984-12-21 1996-11-27 バイオジェン インコーポレイテッド 腫瘍壊死因子の精製、製造および使用法
JPS6248634A (ja) * 1985-07-23 1987-03-03 Mochida Pharmaceut Co Ltd 新規アミノ酸組成物、その製造方法及び該アミノ酸組成物を含有する医療組成物
JPH064675B2 (ja) * 1985-07-29 1994-01-19 伝一 水野 抗腫瘍性ポリペプチド
US5059530A (en) * 1985-09-30 1991-10-22 Suntory Ltd. Expression vector for human TNF
JPH0698004B2 (ja) * 1985-09-30 1994-12-07 サントリー株式会社 Ｔｎｆ発現用新規プラスミド
US4677197A (en) * 1985-10-30 1987-06-30 Cetus Corporation Purification method for tumor necrosis factor
CA1286843C (en) * 1985-10-30 1991-07-23 Leo S. Lin Purification method for proteins
US4894439A (en) * 1986-05-22 1990-01-16 Cetus Corporation N-terminal derivatives of tumor necrosis factor purified by microporous PTFE membranes
US5081021A (en) * 1986-02-04 1992-01-14 Mizuno Den Ichi DNA encoding HTNF variants exhibiting enhanced activity
KR940005589B1 (ko) * 1986-04-02 1994-06-21 다이닛뽄세이야꾸 가부시끼가이샤 핵산 또는 엔도톡신의 제거제 및 제거방법
US5425940A (en) * 1986-04-09 1995-06-20 Cetus Oncology Corporation Combination therapy using interleukin-2 and tumor necrosis factor
DE3750056T2 (de) * 1986-06-20 1995-04-06 Dainippon Pharmaceutical Co Polypeptid-Mutanten des menschlichen TNF und für diese Mutanten codierende DNS.
WO1988006625A2 (en) * 1987-02-26 1988-09-07 Cetus Corporation Arginine-depleted human tumor necrosis factor
DE3716513A1 (de) * 1987-05-16 1988-11-24 Basf Ag Proteine mit tnf-wirkung
JP2828988B2 (ja) * 1988-04-03 1998-11-25 源一郎 杣 新規ｄｎａとその生産方法、それを有する新規プラスミド、新規ポリペプチドとその生産方法、及び該ポリペプチドからなる新規抗腫瘍剤
GB2217325B (en) * 1988-04-08 1991-11-20 British Bio Technology Synthetic gene for tumour necrosis factor alpha.
JPH0797997B2 (ja) * 1988-04-28 1995-10-25 帝人株式会社 新規生理活性ポリペプチド
DE3843534A1 (de) * 1988-12-23 1990-07-12 Basf Ag Neue tnf-polypeptide
US5998378A (en) * 1989-08-16 1999-12-07 Chiron Corporation Compositions for the inhibition of TNF hormone formation and uses thereof
US5843693A (en) * 1989-08-16 1998-12-01 Chiron Corporation Assay method for screening for inhibitors of proTNF conversion
DK0491878T3 (da) 1989-08-16 1997-06-23 Chiron Corp Præparater til inhibering af proteinhormondannelse og anvendelser deraf
US6586222B1 (en) 1989-08-16 2003-07-01 Chiron Corporation Recombinant PR-3 and compositions thereof
WO1991017180A1 (en) * 1990-04-27 1991-11-14 Peptide Technology Ltd Peptides derived from human tumour necrosis factor alpha and useful against intracellular malarial parasites
KR970005042B1 (ko) * 1993-02-09 1997-04-11 한일합성섬유공업 주식회사 종양괴사인자-알파 뮤테인
CA2119089A1 (en) * 1993-03-29 1994-09-30 David Banner Tumor necrosis factor muteins
WO1995024501A1 (en) 1994-03-07 1995-09-14 Cetus Oncology Corporation Compositions for the inhibition of tnf formation and uses thereof
US6395273B1 (en) 1998-06-10 2002-05-28 Promega Corporation Prevention and treatment of inflammatory bowel disease
ES2151403B1 (es) * 1998-07-13 2001-07-01 Hernandez Bronchud Miguel Linea celular transformada de rata productora de tnf-alfa humano recombinante.
US20030086903A1 (en) 2001-11-02 2003-05-08 Genvec, Inc. Therapeutic regimen for treating cancer
DK2953634T3 (da) 2013-02-07 2021-08-30 Massachusetts Gen Hospital Fremgangsmåder til udvidelse eller udtømning af regulerende t-celler
US11312922B2 (en) 2019-04-12 2022-04-26 Ecolab Usa Inc. Antimicrobial multi-purpose cleaner comprising a sulfonic acid-containing surfactant and methods of making and using the same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4879226A (en) * 1984-04-06 1989-11-07 Asahi Kasei Kogyo Kabushiki Kaisha Novel human physiologically active polypeptide
JPS60260522A (ja) * 1984-06-07 1985-12-23 Asahi Chem Ind Co Ltd 遺伝子組換体が生産する生理活性物質の安定化法
GR851626B (es) * 1984-07-05 1985-11-26 Genentech Inc
DE3581730D1 (de) * 1984-10-15 1991-03-14 Cetus Corp Menschlicher tumornekrosisfaktor.
JPS61124392A (ja) * 1984-11-22 1986-06-12 Asahi Chem Ind Co Ltd 遺伝子組換体の産生する生理活性物質の精製法

Also Published As

Publication number Publication date
ES8703490A1 (es) 1987-02-16
FR99C0037I2 (es) 2000-10-27
DE19975063I2 (de) 2005-08-18
KR920010225B1 (ko) 1992-11-21
DK172013B1 (da) 1997-09-15
DE3582183D1 (de) 1991-04-25
DK100985A (da) 1985-09-07
ES540939A0 (es) 1988-04-01
KR850006874A (ko) 1985-10-21
PH22108A (en) 1988-06-01
HU202921B (en) 1991-04-29
DE19975063I1 (de) 2005-07-14
AU584608B2 (en) 1989-06-01
DK100985D0 (da) 1985-03-05
NL990034I2 (nl) 2000-11-01
EP0155549A3 (en) 1986-12-30
EP0155549B1 (en) 1991-03-20
NL990034I1 (nl) 1999-12-01
ES550939A0 (es) 1987-02-16
HUT37957A (en) 1986-03-28
CA1341240C (en) 2001-06-05
FR99C0037I1 (es) 1999-12-10
EP0155549A2 (en) 1985-09-25
AU3944885A (en) 1985-09-12

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Legal Events

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FD2A Announcement of lapse in spain

Effective date: 20151230