ES8800353A1 - Procedimiento de clonacion de factor humano viii-r. - Google Patents

Procedimiento de clonacion de factor humano viii-r.

Info

Publication number
ES8800353A1
ES8800353A1 ES555490A ES555490A ES8800353A1 ES 8800353 A1 ES8800353 A1 ES 8800353A1 ES 555490 A ES555490 A ES 555490A ES 555490 A ES555490 A ES 555490A ES 8800353 A1 ES8800353 A1 ES 8800353A1
Authority
ES
Spain
Prior art keywords
cdna
von willebrand
factor
molecular cloning
viiir
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES555490A
Other languages
English (en)
Other versions
ES555490A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Meloy Laboratories Inc
Original Assignee
Meloy Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meloy Laboratories Inc filed Critical Meloy Laboratories Inc
Publication of ES8800353A1 publication Critical patent/ES8800353A1/es
Publication of ES555490A0 publication Critical patent/ES555490A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/36—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODO DE CLONACION DE FACTOR HUMANO VIIIR, O SUS FRAGMENTOS EN UN SISTEMA HUESPED RECOMBINANTE ANTERREPLICANTE. CONSISTE EN LA EXTRACCION DE ARN CELULAR A PARTIR DE CULTIVOS DE CELULAS ENDOTELIALES HUMANAS Y ENRIQUECER LA MEZCLA DE ARN-M ENRIQUECIDO CON UNA MEZCLA QUE CONTIENE LA TRANSCRIPTASA INVERSA DE VIRUS DE MIELOBLASTOSIS AVIAR Y UN INICIADOR OLIGO (DY)18; HIDROLIZAR EL ARN EN CONDICIONES ALCALINAS Y FORMAR UNA SEGUNDA CADENA DE ADN-C MEDIANTE INICIACION DE BUCLE DE HORQUILLA Y SINTESIS DIRIGIDA POR SUBUNIDADES GRANDES DE ADN POLIMERASA I DE E. COLI; INCORPORAR EL ADN-C-DC EN UN VECTOR; E IDENTIFICAR LOS VECTORES, EN QUE SE HA INCORPORADO EL ADN-C-DC MEDIANTE TRANSCRIPCION Y TRADUCCION IN VITRO DE SUS FRAGMENTOS Y DETECCION DE LOS PRODUCTOS DE TRADUCCION POR VIA INMUNOLOGICA.
ES555490A 1985-05-16 1986-05-16 Procedimiento de clonacion de factor humano viii-r. Expired ES8800353A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US73491285A 1985-05-16 1985-05-16

Publications (2)

Publication Number Publication Date
ES8800353A1 true ES8800353A1 (es) 1987-11-01
ES555490A0 ES555490A0 (es) 1987-11-01

Family

ID=24953560

Family Applications (1)

Application Number Title Priority Date Filing Date
ES555490A Expired ES8800353A1 (es) 1985-05-16 1986-05-16 Procedimiento de clonacion de factor humano viii-r.

Country Status (5)

Country Link
EP (1) EP0222003A4 (es)
JP (1) JPS63500144A (es)
AU (1) AU602487B2 (es)
ES (1) ES8800353A1 (es)
WO (1) WO1986006745A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8597910B1 (en) 1985-04-11 2013-12-03 Children's Medical Center Corporation DNA encoding Von Willebrand Factor (VWF) and methods and cells for producing VFW, and VFW produced by the DNA, methods and cells
JPS62502589A (ja) * 1985-04-11 1987-10-08 ザ・チヤイルドレンズ・メデイカル・センタ−・コ−ポレ−シヨン フオン・ビルブラント因子
US5198349A (en) * 1986-01-03 1993-03-30 Genetics Institute, Inc. Method for producing factor VIII:C and analogs
US5849536A (en) * 1990-03-02 1998-12-15 Bio-Technology General Corp. Cloning and production of human von willebrand factor GPIb binding domain polypeptides and methods of using same
US6008193A (en) * 1990-03-02 1999-12-28 Bio-Technology General Corp. Methods of using human von Willebrand factor GPIb binding domain polypeptides
US5880327A (en) * 1994-09-21 1999-03-09 American National Red Cross Transgenic mammals expressing human coagulation factor VIII
US6066778A (en) * 1996-11-06 2000-05-23 The Regents Of The University Of Michigan Transgenic mice expressing APC resistant factor V
AU7619500A (en) * 1999-09-28 2001-04-30 Us Transgenics, Inc. Transgenic vwf and vwf-related polypeptides, transgenics, methods, compositions,uses and the like relating thereto

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI852866L (fi) * 1984-07-27 1986-01-28 Meloy Lab Rekombinantfaktor viii-r.
NL8500961A (nl) * 1985-04-01 1986-11-03 Stichting Vrienden Van De Stic Cdna-codering voor de humane von willebrand-factor, plasmiden met een dergelijke cdna-codering respektievelijk fragmenten ervan, alsmede micro-organismen, welke dergelijke plasmiden bevatten.
JPS62502589A (ja) * 1985-04-11 1987-10-08 ザ・チヤイルドレンズ・メデイカル・センタ−・コ−ポレ−シヨン フオン・ビルブラント因子

Also Published As

Publication number Publication date
EP0222003A1 (en) 1987-05-20
JPS63500144A (ja) 1988-01-21
WO1986006745A1 (en) 1986-11-20
ES555490A0 (es) 1987-11-01
EP0222003A4 (en) 1989-03-23
AU602487B2 (en) 1990-10-18
AU5900586A (en) 1986-12-04

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