ES8606489A1 - Un metodo de producir una proteina de interleuquina-2-humana no glicosilada sustancialmente pura - Google Patents
Un metodo de producir una proteina de interleuquina-2-humana no glicosilada sustancialmente puraInfo
- Publication number
- ES8606489A1 ES8606489A1 ES537994A ES537994A ES8606489A1 ES 8606489 A1 ES8606489 A1 ES 8606489A1 ES 537994 A ES537994 A ES 537994A ES 537994 A ES537994 A ES 537994A ES 8606489 A1 ES8606489 A1 ES 8606489A1
- Authority
- ES
- Spain
- Prior art keywords
- human interleukin
- protein
- glycosylated human
- esp
- column chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Abstract
UN METODO DE PRODUCIR UNA PROTEINA DE INTERLEUQUINA-2 HUMANA NO GLICOSILADA, SUSTANCIALMENTE PURA. SE OBTIENE CULTIVANDO UN TRANSFORMANTE QUE LLEVA UN ADN QUE TIENE UNA SECUENCIA DE BASES QUE CODIFICA INTERLEUQUINA-2 HUMANA PARA CAUSAR LA PRODUCCION Y ACUMULACION DE INTERLEUQUINA-2 HUMANA EN EL CALDO DE CULTIVO, Y SOMETIENDO EL LIQUIDO QUE CONTIENE INTERLEUQUINA-2 HUMANA, ASI OBTENIDO, A UN PROCEDIMIENTO DE PURIFICACION QUE COMPRENDE UNA CROMATOGRAFIA EN COLUMNA HIDROFOBA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP58225079A JPS60115528A (ja) | 1983-11-28 | 1983-11-28 | ヒトインタ―ロイキン―2蛋白質を含有する抗腫瘍用または免疫機能低下疾患治療用組成物 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES537994A0 ES537994A0 (es) | 1986-04-01 |
ES8606489A1 true ES8606489A1 (es) | 1986-04-01 |
Family
ID=16823684
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES537994A Expired ES8606489A1 (es) | 1983-11-28 | 1984-11-27 | Un metodo de producir una proteina de interleuquina-2-humana no glicosilada sustancialmente pura |
Country Status (16)
Country | Link |
---|---|
US (1) | US5464939A (es) |
EP (3) | EP0442538A1 (es) |
JP (1) | JPS60115528A (es) |
KR (1) | KR920006879B1 (es) |
DK (1) | DK164174C (es) |
ES (1) | ES8606489A1 (es) |
FI (1) | FI81118C (es) |
GR (1) | GR81040B (es) |
HU (1) | HU200793B (es) |
IL (1) | IL73530A0 (es) |
MY (1) | MY103892A (es) |
NO (1) | NO169021C (es) |
NZ (1) | NZ210352A (es) |
PT (1) | PT79555B (es) |
SG (1) | SG46452A1 (es) |
ZA (1) | ZA849221B (es) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4925919A (en) * | 1984-04-25 | 1990-05-15 | Roland Mertelsmann | Purified interleukin 2 |
US4992271A (en) * | 1982-09-23 | 1991-02-12 | Cetus Corporation | Formulation for lipophilic IL-2 proteins |
US4853332A (en) * | 1982-10-19 | 1989-08-01 | Cetus Corporation | Structural genes, plasmids and transformed cells for producing cysteine depleted muteins of biologically active proteins |
ZA849910B (en) * | 1983-12-23 | 1985-09-25 | Hoffmann La Roche | Purification of recombinant interleukin-2 |
US4569790A (en) * | 1984-03-28 | 1986-02-11 | Cetus Corporation | Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions |
JPS60243021A (ja) * | 1984-03-28 | 1985-12-03 | シタス コ−ポレイシヨン | システイン含有蛋白質の制御された酸化方法 |
DE3583880D1 (de) * | 1984-04-09 | 1991-10-02 | Takeda Chemical Industries Ltd | Stabile interleukin-2-zusammensetzung. |
US4908433A (en) * | 1984-04-25 | 1990-03-13 | Sloan-Kettering Institute For Cancer Research | Uses of interleukin-2 |
US4908434A (en) * | 1984-04-25 | 1990-03-13 | Sloan-Kettering Institute For Cancer Research | Process for preparing purified interleukin-2 |
WO1986002068A1 (en) * | 1984-09-26 | 1986-04-10 | Takeda Chemical Industries, Ltd. | Mutual separation of proteins |
IL76360A0 (en) | 1984-09-26 | 1986-01-31 | Takeda Chemical Industries Ltd | Mutual separation of proteins |
JPH0646957B2 (ja) * | 1985-03-11 | 1994-06-22 | 武田薬品工業株式会社 | インタ−ロイキン−2の製造方法 |
EP0226639B1 (en) * | 1985-05-29 | 1990-11-14 | The Green Cross Corporation | Process for preparing heterogenic protein |
US4748234A (en) * | 1985-06-26 | 1988-05-31 | Cetus Corporation | Process for recovering refractile bodies containing heterologous proteins from microbial hosts |
EP0227833B1 (en) * | 1985-07-16 | 1991-01-16 | Green Cross Corporation | Process for preparing hetero-protein |
DE3526096A1 (de) * | 1985-07-22 | 1987-01-22 | Basf Ag | Verfahren zur reinigung von htnf |
CN86104525A (zh) * | 1985-07-31 | 1987-02-25 | 武田药品工业株式会社 | 人类白细胞介素-2的分析方法和试剂 |
DK585886A (da) * | 1985-12-24 | 1987-06-25 | Takeda Chemical Industries Ltd | Immunstimulerende middel og anvendelse deraf |
US4933433A (en) * | 1986-01-31 | 1990-06-12 | E. I. Du Pont De Nemours And Company | Recombinant interleukin-2 composition and process for making it |
JPH0655153B2 (ja) * | 1986-02-26 | 1994-07-27 | 武田薬品工業株式会社 | ヒトインターロイキン―2結晶の製造法 |
US4929700A (en) * | 1987-04-16 | 1990-05-29 | Cetus Corporation | Production of purified, biologically active, bacterially produced recombinant human CSF-1 |
CA1339757C (en) | 1987-04-16 | 1998-03-17 | Robert F. Halenbeck | Production of purified biologically active, bacterially produced recombinant human csf-1 |
US4961969A (en) * | 1987-05-11 | 1990-10-09 | Cetus Corporation | Process for recovering microbially produced interferon-β |
US4931543A (en) * | 1987-05-11 | 1990-06-05 | Cetus Corporation | Process for recovering microbially produced interleukin-2 |
US5162503A (en) * | 1987-05-19 | 1992-11-10 | Hoffmann-La Roche, Inc. | Purification of interleukin-2 by receptor-affinity chromatography |
US5149788A (en) * | 1987-05-19 | 1992-09-22 | Hoffmann-La Roche, Inc. | Purification of chimeric proteins containing an IL-2 moiety by receptor-affinity chromatography |
FR2635527B1 (fr) * | 1988-07-28 | 1992-06-12 | Roussel Uclaf | Il2 humaine recombinante non glycosylee sous forme reduite, son procede d'obtention et son application comme medicament |
US5250296A (en) * | 1990-11-29 | 1993-10-05 | Takeda Chemical Industries, Ltd. | Immunostimulant agent containing interleukin-2 and 5'-deoxy-5-fluorouridine |
US5416071A (en) * | 1991-03-12 | 1995-05-16 | Takeda Chemical Industries, Ltd. | Water-soluble composition for sustained-release containing epo and hyaluronic acid |
ES2545090T3 (es) | 2001-12-21 | 2015-09-08 | Human Genome Sciences, Inc. | Proteínas de fusión de albúmina y GCSF |
GB201000693D0 (en) | 2010-01-15 | 2010-03-03 | Isis Innovation | A solar cell |
EA037083B1 (ru) * | 2010-11-12 | 2021-02-03 | Нектар Терапьютикс | Иммуномодулирующий конъюгат, включающий il-2 и водорастворимые полимеры |
CN113230384A (zh) | 2014-10-09 | 2021-08-10 | 丹娜法伯癌症研究院 | 用于治疗免疫失调的多次-可变il-2剂量方案 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58116498A (ja) * | 1981-12-28 | 1983-07-11 | Takeda Chem Ind Ltd | Il‐2をコードする新規伝令rnaの製造法 |
JPH0751511B2 (ja) * | 1982-03-15 | 1995-06-05 | 味の素株式会社 | インターロイキン2を含有してなる癌治療剤 |
CA1210714A (en) * | 1982-03-23 | 1986-09-02 | Alan Sloma | .alpha.-INTERFERON GX-1 |
JPS58168492A (ja) * | 1982-03-31 | 1983-10-04 | Nippon Kokan Kk <Nkk> | パイプ切断機 |
DE3377363D1 (en) * | 1982-03-31 | 1988-08-18 | Ajinomoto Kk | Gene coding for interleukin-2 polypeptide, recombinant dna carrying said gene, cell lines possessing the recombinant dna,and method for producing interleukin-2 using said cells |
CA1341562C (en) * | 1982-03-31 | 2007-11-27 | Tadatsugu Taniguchi | Gene coded for interleukin-2 polypeptide, recombinant dna carrying the said gene, a living cell line possessing the recombinant dna, and method for producing interleukin-2 using the said cell |
JPS58201979A (ja) * | 1982-05-18 | 1983-11-25 | Japan Found Cancer | ヒトインタ−ロイキン2遺伝子が組込まれているエシエリヒア・コリおよびその育種方法 |
ATE37564T1 (de) * | 1982-04-20 | 1988-10-15 | Sloan Kettering Inst Cancer | Reinigung von interleukin-2. |
JPS58198293A (ja) * | 1982-05-12 | 1983-11-18 | Shionogi & Co Ltd | インタ−ロイキン等免疫調節因子の製造方法 |
NL8203321A (nl) * | 1982-08-25 | 1984-03-16 | Philips Nv | Kleurenbeeldbuis. |
US4490289A (en) * | 1982-09-16 | 1984-12-25 | Hoffmann-La Roche Inc. | Homogeneous human interleukin 2 |
AU579089B2 (en) * | 1983-02-08 | 1988-11-17 | Biogen, Inc. | Human interleukin-2-like polypeptides |
IL71275A0 (en) * | 1983-03-21 | 1984-06-29 | Sparamedica Ag | Human interleukin-2-and its preparation |
US4518584A (en) * | 1983-04-15 | 1985-05-21 | Cetus Corporation | Human recombinant interleukin-2 muteins |
EP0162898A4 (en) * | 1983-11-14 | 1987-07-23 | Chiron Corp | INTERLEUKIN-2 GENERATION USING CLONED GENES FOR INTERLEUKIN-2 AND YEAR ALPHA FACTOR. |
ZA849910B (en) * | 1983-12-23 | 1985-09-25 | Hoffmann La Roche | Purification of recombinant interleukin-2 |
US4569790A (en) * | 1984-03-28 | 1986-02-11 | Cetus Corporation | Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions |
US4530787A (en) * | 1984-03-28 | 1985-07-23 | Cetus Corporation | Controlled oxidation of microbially produced cysteine-containing proteins |
JP2608730B2 (ja) * | 1987-08-10 | 1997-05-14 | 株式会社リコー | 感熱記録材料 |
-
1983
- 1983-11-28 JP JP58225079A patent/JPS60115528A/ja active Granted
-
1984
- 1984-11-16 IL IL73530A patent/IL73530A0/xx unknown
- 1984-11-23 SG SG1996004789A patent/SG46452A1/en unknown
- 1984-11-23 EP EP19910105904 patent/EP0442538A1/en not_active Withdrawn
- 1984-11-23 EP EP96201538A patent/EP0751220A1/en not_active Ceased
- 1984-11-23 EP EP84308153A patent/EP0145390A3/en not_active Ceased
- 1984-11-26 ZA ZA849221A patent/ZA849221B/xx unknown
- 1984-11-26 GR GR81040A patent/GR81040B/el unknown
- 1984-11-27 NO NO844710A patent/NO169021C/no unknown
- 1984-11-27 NZ NZ210352A patent/NZ210352A/xx unknown
- 1984-11-27 PT PT79555A patent/PT79555B/pt not_active IP Right Cessation
- 1984-11-27 KR KR1019840007430A patent/KR920006879B1/ko not_active IP Right Cessation
- 1984-11-27 ES ES537994A patent/ES8606489A1/es not_active Expired
- 1984-11-27 DK DK560784A patent/DK164174C/da not_active IP Right Cessation
- 1984-11-27 HU HU844383A patent/HU200793B/hu not_active IP Right Cessation
- 1984-11-28 FI FI844678A patent/FI81118C/fi not_active IP Right Cessation
-
1987
- 1987-09-28 MY MYPI87002002A patent/MY103892A/en unknown
-
1992
- 1992-09-09 US US07/942,358 patent/US5464939A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
FI81118C (fi) | 1990-09-10 |
FI844678L (fi) | 1985-05-29 |
HUT36186A (en) | 1985-08-28 |
FI81118B (fi) | 1990-05-31 |
KR920006879B1 (ko) | 1992-08-21 |
AU592527B2 (en) | 1990-01-18 |
NO844710L (no) | 1985-05-29 |
EP0145390A2 (en) | 1985-06-19 |
IL73530A0 (en) | 1985-02-28 |
DK164174C (da) | 1992-10-05 |
HU200793B (en) | 1990-08-28 |
NO169021B (no) | 1992-01-20 |
EP0442538A1 (en) | 1991-08-21 |
ZA849221B (en) | 1986-07-30 |
US5464939A (en) | 1995-11-07 |
PT79555A (en) | 1984-12-01 |
KR850003734A (ko) | 1985-06-26 |
FI844678A0 (fi) | 1984-11-28 |
ES537994A0 (es) | 1986-04-01 |
NO169021C (no) | 1992-04-29 |
SG46452A1 (en) | 1998-02-20 |
JPH0542413B2 (es) | 1993-06-28 |
AU3575684A (en) | 1985-06-06 |
EP0145390A3 (en) | 1987-01-21 |
JPS60115528A (ja) | 1985-06-22 |
NZ210352A (en) | 1988-11-29 |
EP0751220A1 (en) | 1997-01-02 |
MY103892A (en) | 1993-10-30 |
DK560784D0 (da) | 1984-11-27 |
DK560784A (da) | 1985-05-29 |
GR81040B (en) | 1985-03-27 |
PT79555B (en) | 1986-12-15 |
DK164174B (da) | 1992-05-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19970401 |