ES2460896T3 - Amplificación de ácido nucleico - Google Patents

Amplificación de ácido nucleico Download PDF

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Publication number
ES2460896T3
ES2460896T3 ES08800110.2T ES08800110T ES2460896T3 ES 2460896 T3 ES2460896 T3 ES 2460896T3 ES 08800110 T ES08800110 T ES 08800110T ES 2460896 T3 ES2460896 T3 ES 2460896T3
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ES
Spain
Prior art keywords
primer
oligonucleotide
facilitator
sequence
facilitator oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
ES08800110.2T
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English (en)
Spanish (es)
Inventor
Keith Norman Rand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Filing date
Publication date
Priority claimed from AU2007905445A external-priority patent/AU2007905445A0/en
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
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Publication of ES2460896T3 publication Critical patent/ES2460896T3/es
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES08800110.2T 2007-10-04 2008-10-03 Amplificación de ácido nucleico Active ES2460896T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2007905445A AU2007905445A0 (en) 2007-10-04 Nucleic acid amplification
AU2007905445 2007-10-04
PCT/AU2008/001475 WO2009043112A1 (en) 2007-10-04 2008-10-03 Nucleic acid amplification

Publications (1)

Publication Number Publication Date
ES2460896T3 true ES2460896T3 (es) 2014-05-14

Family

ID=40525777

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08800110.2T Active ES2460896T3 (es) 2007-10-04 2008-10-03 Amplificación de ácido nucleico

Country Status (7)

Country Link
US (1) US8455197B2 (enExample)
EP (1) EP2207896B1 (enExample)
JP (1) JP5535921B2 (enExample)
CN (1) CN101889096B (enExample)
AU (1) AU2008307153B2 (enExample)
ES (1) ES2460896T3 (enExample)
WO (1) WO2009043112A1 (enExample)

Families Citing this family (34)

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WO2009114543A2 (en) * 2008-03-11 2009-09-17 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
WO2011009073A1 (en) * 2009-07-16 2011-01-20 Abbott Laboratories A method for amplification of nucleic acids
DK2516680T3 (en) 2009-12-22 2016-05-02 Sequenom Inc Method and kits to identify aneuploidy
EP2702168B1 (en) 2011-04-29 2018-01-17 Sequenom, Inc. Quantification of a minority nucleic acid species
WO2013075079A1 (en) * 2011-11-17 2013-05-23 Rheonix, Inc. System and methods for selective molecular analysis
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
DK4001436T3 (da) 2012-05-11 2024-05-27 Clinical Genomics Pty Ltd Diagnostisk genmarkørpanel
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
EP2872648B1 (en) 2012-07-13 2019-09-04 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
CN109913519B (zh) * 2012-12-13 2022-12-02 合成基因组股份有限公司 Peg-介导的核酸分子的组装
WO2014168711A1 (en) 2013-03-13 2014-10-16 Sequenom, Inc. Primers for dna methylation analysis
US10202629B2 (en) * 2013-03-15 2019-02-12 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids utilizing clamp oligonucleotides
EP2971134B1 (en) * 2013-03-15 2023-10-25 Aegea Biotechnologies, Inc. Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence
EP3715470B1 (en) * 2013-08-09 2023-10-11 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US11365447B2 (en) 2014-03-13 2022-06-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR102343605B1 (ko) * 2014-05-19 2021-12-27 윌리엄 마쉬 라이스 유니버시티 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭
CN104131070A (zh) * 2014-05-22 2014-11-05 苏州工业园区为真生物医药科技有限公司 一种对基因甲基化检测的方法
WO2016061624A1 (en) 2014-10-20 2016-04-28 Commonwealth Scientific And Industrial Research Organisation Genome methylation analysis
CN105331727B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
US10443095B2 (en) 2016-08-02 2019-10-15 Roche Molecular Systems, Inc. Helper oligonucleotide for improved efficiency of amplification and detection/quantitation of nucleic acids
CN118562942A (zh) 2016-09-06 2024-08-30 合成Dna技术公司 Ngs文库浓度的标准化
DE102016120124B8 (de) 2016-10-21 2018-08-23 Gna Biosolutions Gmbh Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens
US10787699B2 (en) * 2017-02-08 2020-09-29 Microsoft Technology Licensing, Llc Generating pluralities of primer and payload designs for retrieval of stored nucleotides
EP3530754A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
EP3530753A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
US20210071243A1 (en) * 2018-02-26 2021-03-11 Agct Gmbh Method for primer extension reaction with improved specificity
JP2021514637A (ja) * 2018-02-26 2021-06-17 アーゲーツェーテー ゲーエムベーハーAgct Gmbh 特異性を改善した核酸を増幅する方法
US20210071244A1 (en) * 2018-02-28 2021-03-11 Agct Gmbh Method for the amplification of a nucleic acid with improved specificity
CN114075595B (zh) * 2021-11-22 2023-11-17 上海交通大学 甲基化检测组合物、试剂盒及方法
CN115948526A (zh) * 2022-11-30 2023-04-11 华中科技大学 用于CRISPR-Cas12a体系的底物DNA及其应用和通用型基因突变检测方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335184B1 (en) * 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
DE69827060T2 (de) * 1997-03-20 2005-03-24 F. Hoffmann-La Roche Ag Modifizierte Primer
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
JPWO2003016569A1 (ja) * 2001-08-20 2004-12-02 タカラバイオ株式会社 核酸の増幅方法
US7112406B2 (en) * 2002-03-01 2006-09-26 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
US20060014183A1 (en) * 2004-06-10 2006-01-19 Pfundheller Henrik M Extendable probes
US8501403B2 (en) 2006-03-28 2013-08-06 Commonwealth Scientific And Industrial Research Organisation Amplification of DNA fragments
WO2008064687A1 (en) 2006-11-27 2008-06-05 Fluimedix Fidelity enhanced allele specific amplification method

Also Published As

Publication number Publication date
JP2010539956A (ja) 2010-12-24
US8455197B2 (en) 2013-06-04
AU2008307153A1 (en) 2009-04-09
WO2009043112A1 (en) 2009-04-09
AU2008307153B2 (en) 2015-03-12
EP2207896A1 (en) 2010-07-21
CN101889096A (zh) 2010-11-17
JP5535921B2 (ja) 2014-07-02
EP2207896B1 (en) 2014-01-29
EP2207896A4 (en) 2010-11-10
CN101889096B (zh) 2015-10-21
US20110033851A1 (en) 2011-02-10

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