CN101889096B - 核酸扩增 - Google Patents

核酸扩增 Download PDF

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Publication number
CN101889096B
CN101889096B CN200880119335.8A CN200880119335A CN101889096B CN 101889096 B CN101889096 B CN 101889096B CN 200880119335 A CN200880119335 A CN 200880119335A CN 101889096 B CN101889096 B CN 101889096B
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CN
China
Prior art keywords
primer
oligonucleotide
sequence
helper
nucleic acid
Prior art date
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Expired - Fee Related
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CN200880119335.8A
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English (en)
Chinese (zh)
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CN101889096A (zh
Inventor
基思·N·兰德
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Priority claimed from AU2007905445A external-priority patent/AU2007905445A0/en
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Publication of CN101889096A publication Critical patent/CN101889096A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200880119335.8A 2007-10-04 2008-10-03 核酸扩增 Expired - Fee Related CN101889096B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2007905445 2007-10-04
AU2007905445A AU2007905445A0 (en) 2007-10-04 Nucleic acid amplification
PCT/AU2008/001475 WO2009043112A1 (en) 2007-10-04 2008-10-03 Nucleic acid amplification

Publications (2)

Publication Number Publication Date
CN101889096A CN101889096A (zh) 2010-11-17
CN101889096B true CN101889096B (zh) 2015-10-21

Family

ID=40525777

Family Applications (1)

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CN200880119335.8A Expired - Fee Related CN101889096B (zh) 2007-10-04 2008-10-03 核酸扩增

Country Status (7)

Country Link
US (1) US8455197B2 (enExample)
EP (1) EP2207896B1 (enExample)
JP (1) JP5535921B2 (enExample)
CN (1) CN101889096B (enExample)
AU (1) AU2008307153B2 (enExample)
ES (1) ES2460896T3 (enExample)
WO (1) WO2009043112A1 (enExample)

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EP2271772B1 (en) * 2008-03-11 2014-07-16 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP2454381A1 (en) * 2009-07-16 2012-05-23 Abbott Laboratories A method for amplification of nucleic acids
EP2516680B1 (en) 2009-12-22 2016-04-06 Sequenom, Inc. Processes and kits for identifying aneuploidy
CN103717750B (zh) 2011-04-29 2017-03-08 塞昆纳姆股份有限公司 少数核酸物质的定量
EP2780474A4 (en) * 2011-11-17 2015-06-17 Rheonix Inc SYSTEM AND METHOD FOR SELECTIVE MOLECULAR ANALYSIS
EP3757210B1 (en) 2012-03-02 2022-08-24 Sequenom, Inc. Methods for enriching cancer nucleic acid from a biological sample
US11254985B2 (en) 2012-05-11 2022-02-22 Clinical Genomics Pty. Ltd. Diagnostic gene marker panel for colorectal cancer
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
EP2872648B1 (en) 2012-07-13 2019-09-04 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
CN109913519B (zh) * 2012-12-13 2022-12-02 合成基因组股份有限公司 Peg-介导的核酸分子的组装
US11060145B2 (en) 2013-03-13 2021-07-13 Sequenom, Inc. Methods and compositions for identifying presence or absence of hypermethylation or hypomethylation locus
EP2971134B1 (en) * 2013-03-15 2023-10-25 Aegea Biotechnologies, Inc. Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence
CA2905527C (en) * 2013-03-15 2023-10-03 Lyle J. Arnold Methods for amplification of nucleic acids utilizing clamp oligonucleotides
EP4321867B1 (en) * 2013-08-09 2025-10-01 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
EP3736344A1 (en) 2014-03-13 2020-11-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR102343605B1 (ko) * 2014-05-19 2021-12-27 윌리엄 마쉬 라이스 유니버시티 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭
CN104131070A (zh) * 2014-05-22 2014-11-05 苏州工业园区为真生物医药科技有限公司 一种对基因甲基化检测的方法
EP3209801B1 (en) 2014-10-20 2023-06-28 Commonwealth Scientific and Industrial Research Organisation Genome methylation analysis
CN105331727B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
WO2018024562A1 (en) * 2016-08-02 2018-02-08 Roche Diagnostics Gmbh Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids
CN118562942A (zh) 2016-09-06 2024-08-30 合成Dna技术公司 Ngs文库浓度的标准化
DE102016120124B8 (de) 2016-10-21 2018-08-23 Gna Biosolutions Gmbh Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens
US10787699B2 (en) * 2017-02-08 2020-09-29 Microsoft Technology Licensing, Llc Generating pluralities of primer and payload designs for retrieval of stored nucleotides
EP3530754A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
EP3530753A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
US20210071243A1 (en) * 2018-02-26 2021-03-11 Agct Gmbh Method for primer extension reaction with improved specificity
CN112074612A (zh) * 2018-02-26 2020-12-11 Agct有限公司 一种具有更高特异性的核酸扩增方法
EP3759246A1 (de) * 2018-02-28 2021-01-06 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
CN114075595B (zh) * 2021-11-22 2023-11-17 上海交通大学 甲基化检测组合物、试剂盒及方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335184B1 (en) * 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
WO2003074724A2 (en) * 2002-03-01 2003-09-12 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids

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US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
DE69827060T2 (de) * 1997-03-20 2005-03-24 F. Hoffmann-La Roche Ag Modifizierte Primer
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
KR20040028991A (ko) * 2001-08-20 2004-04-03 다카라 바이오 가부시키가이샤 핵산 증폭 방법
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
US20060014183A1 (en) * 2004-06-10 2006-01-19 Pfundheller Henrik M Extendable probes
ES2357746T3 (es) * 2006-03-28 2011-04-29 Commonwealth Scientific And Industrial Research Organisation Amplificación de fragmentos de adn.
WO2008064687A1 (en) * 2006-11-27 2008-06-05 Fluimedix Fidelity enhanced allele specific amplification method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335184B1 (en) * 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
WO2003074724A2 (en) * 2002-03-01 2003-09-12 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids

Also Published As

Publication number Publication date
US8455197B2 (en) 2013-06-04
EP2207896A1 (en) 2010-07-21
AU2008307153B2 (en) 2015-03-12
EP2207896B1 (en) 2014-01-29
JP2010539956A (ja) 2010-12-24
CN101889096A (zh) 2010-11-17
ES2460896T3 (es) 2014-05-14
EP2207896A4 (en) 2010-11-10
WO2009043112A1 (en) 2009-04-09
US20110033851A1 (en) 2011-02-10
AU2008307153A1 (en) 2009-04-09
JP5535921B2 (ja) 2014-07-02

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