DE102016120124B8 - Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens - Google Patents
Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens Download PDFInfo
- Publication number
- DE102016120124B8 DE102016120124B8 DE102016120124.3A DE102016120124A DE102016120124B8 DE 102016120124 B8 DE102016120124 B8 DE 102016120124B8 DE 102016120124 A DE102016120124 A DE 102016120124A DE 102016120124 B8 DE102016120124 B8 DE 102016120124B8
- Authority
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- Germany
- Prior art keywords
- conducting
- carrying
- chain reaction
- polymerase chain
- polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000000034 method Methods 0.000 title 2
- 238000003752 polymerase chain reaction Methods 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/08—Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1811—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using electromagnetic induction heating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
- B01L2400/0445—Natural or forced convection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/30—Characterised by physical treatment
- C12Q2523/307—Denaturation or renaturation by electric current/voltage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/137—Metal/ion, e.g. metal label
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Control Of Resistance Heating (AREA)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102016120124.3A DE102016120124B8 (de) | 2016-10-21 | 2016-10-21 | Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens |
| CA3041042A CA3041042A1 (en) | 2016-10-21 | 2017-10-20 | Method for carrying out a polymerase chain reaction and device for carrying out the method |
| JP2019520521A JP7140759B2 (ja) | 2016-10-21 | 2017-10-20 | ポリメラーゼ連鎖反応を実行するための方法および該方法を実行するための装置 |
| AU2017345281A AU2017345281A1 (en) | 2016-10-21 | 2017-10-20 | Method for carrying out a polymerase chain reaction and device for carrying out the method |
| PCT/EP2017/076902 WO2018073435A1 (en) | 2016-10-21 | 2017-10-20 | Method for carrying out a polymerase chain reaction and device for carrying out the method |
| KR1020197014410A KR20190082233A (ko) | 2016-10-21 | 2017-10-20 | 중합효소연쇄반응의 수행방법 및 상기 방법을 수행하기 위한 장치 |
| EP17797248.6A EP3528954B1 (de) | 2016-10-21 | 2017-10-20 | Verfahren zur durchführung einer polymerasekettenreaktion und vorrichtung zur durchführung des verfahrens |
| EP20174775.5A EP3733292B1 (de) | 2016-10-21 | 2017-10-20 | Verfahren zur durchführung einer polymerasekettenreaktion und vorrichtung zur durchführung des verfahrens |
| US16/343,675 US20200063173A1 (en) | 2016-10-21 | 2017-10-20 | Method for carrying out a polymerase chain reaction and device for carrying out the method |
| ES17797248T ES2890411T3 (es) | 2016-10-21 | 2017-10-20 | Método para llevar a cabo una reacción en cadena de la polimerasa y dispositivo para llevar a cabo el método |
| CN201780065390.2A CN109862965B (zh) | 2016-10-21 | 2017-10-20 | 用于进行聚合酶链式反应的方法和用于进行该方法的装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102016120124.3A DE102016120124B8 (de) | 2016-10-21 | 2016-10-21 | Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| DE102016120124A1 DE102016120124A1 (de) | 2018-04-26 |
| DE102016120124B4 DE102016120124B4 (de) | 2018-07-19 |
| DE102016120124A8 DE102016120124A8 (de) | 2018-08-16 |
| DE102016120124B8 true DE102016120124B8 (de) | 2018-08-23 |
Family
ID=60302067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE102016120124.3A Active DE102016120124B8 (de) | 2016-10-21 | 2016-10-21 | Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20200063173A1 (de) |
| EP (2) | EP3733292B1 (de) |
| JP (1) | JP7140759B2 (de) |
| KR (1) | KR20190082233A (de) |
| CN (1) | CN109862965B (de) |
| AU (1) | AU2017345281A1 (de) |
| CA (1) | CA3041042A1 (de) |
| DE (1) | DE102016120124B8 (de) |
| ES (1) | ES2890411T3 (de) |
| WO (1) | WO2018073435A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102018103215B3 (de) * | 2018-02-13 | 2019-08-14 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zur Extraktion einer Nukleinsäure aus einer Probenflüssigkeit |
| DE102019114011B3 (de) | 2019-05-07 | 2020-07-30 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zum Extrahieren und/oder Vervielfältigen einer Target-Nukleinsäure |
| EP3569717A3 (de) | 2019-08-28 | 2020-04-08 | Boehringer Ingelheim Vetmedica GmbH | Diagnostisches verfahren zur dedektion von dna und rna viren mittels polymerase kettenreaktion in einem beheizten reaktionsvolumen |
| US11149320B1 (en) | 2020-03-31 | 2021-10-19 | Diasorin S.P.A. | Assays for the detection of SARS-CoV-2 |
| WO2021219533A1 (en) | 2020-04-29 | 2021-11-04 | Boehringer Ingelheim Vetmedica Gmbh | Cartridge, system and method for amplification of at least one analyte |
| DE102020118516A1 (de) | 2020-06-26 | 2021-12-30 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zur Extraktion und Amplifikation einer Target-Nukleinsäure |
| CN112501261B (zh) * | 2021-01-29 | 2021-12-07 | 量准（上海）医疗器械有限公司 | 一种纳米等离子共振芯片增强等温扩增核酸荧光检测方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102013215166B3 (de) * | 2013-08-01 | 2014-10-30 | Gna Biosolutions Gmbh | PCR-Verfahren zur Superamplifikation |
| DE102013215168A1 (de) * | 2013-08-01 | 2015-02-05 | Gna Biosolutions Gmbh | Verfahren zum Vervielfältigen von Nukleinsäuren |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| DE19543060A1 (de) | 1995-11-07 | 1997-05-15 | Peter Prof Dr Gruendler | Verfahren zur elektrochemischen Messung an direkt geheizten Elektroden |
| EP1591541B1 (de) | 1997-04-01 | 2012-02-15 | Illumina Cambridge Limited | Verfahren zur Vervielfältigung von Nukleinsäuren |
| DE19960398B4 (de) | 1999-12-15 | 2004-05-06 | Gründler, Peter, Prof. Dr. | Verfahren und elektrochemischer Sensor mit geheizten Elektroden für die biochemische Analytik, insbesondere zur Untersuchung von Hybridisierungsvorgängen der Nucleinsäuren |
| US6586233B2 (en) | 2001-03-09 | 2003-07-01 | The Regents Of The University Of California | Convectively driven PCR thermal-cycling |
| US7164077B2 (en) * | 2001-04-09 | 2007-01-16 | Research Triangle Institute | Thin-film thermoelectric cooling and heating devices for DNA genomic and proteomic chips, thermo-optical switching circuits, and IR tags |
| US6750661B2 (en) * | 2001-11-13 | 2004-06-15 | Caliper Life Sciences, Inc. | Method and apparatus for controllably effecting samples using two signals |
| CN1267565C (zh) * | 2003-06-09 | 2006-08-02 | 清华大学 | 微液滴振荡型硅槽式聚合酶链式反应生物芯片 |
| JP4592060B2 (ja) | 2004-04-26 | 2010-12-01 | キヤノン株式会社 | Ｐｃｒ増幅反応装置、ならびに、該装置を利用するｐｃｒ増幅反応方法 |
| EP1748084A1 (de) * | 2005-07-28 | 2007-01-31 | Roche Diagnostics GmbH | Analyseverfahren und -Vorrichtung |
| EP2029721A4 (de) | 2006-05-30 | 2011-01-26 | Univ Utah Res Found | Gleichzeitige amplifikation und identifizierung von ribonukleinsäure mit einem optischen verfahren unter verwendung von plasmonresonanz |
| WO2009043112A1 (en) * | 2007-10-04 | 2009-04-09 | Commonwealth Scientific And Industrial Research Organisation | Nucleic acid amplification |
| DE102012201475B4 (de) | 2012-02-01 | 2014-12-18 | Gna Biosolutions Gmbh | Verfahren zum Vervielfältigen von Nukleinsäuren |
| CN116064734A (zh) * | 2012-04-19 | 2023-05-05 | 生命技术公司 | 核酸扩增 |
| GB201212775D0 (en) * | 2012-07-18 | 2012-08-29 | Dna Electronics Ltd | Sensing apparatus and method |
| EP2882534A4 (de) * | 2012-08-07 | 2016-04-13 | California Inst Of Techn | Ultraschneller thermocycler |
| US9579655B2 (en) * | 2013-01-18 | 2017-02-28 | Biomeme Incorporated | Analytic device |
| EP2965063A4 (de) * | 2013-03-08 | 2016-09-14 | Otago Innovation Ltd | Reaktionsgefässhalter und moleküldetektionsvorrichtung |
| CN105092543A (zh) * | 2014-05-12 | 2015-11-25 | 绍兴安尼特微电子科技有限公司 | 一种便携式荧光定量pcr检测仪 |
| WO2016163946A1 (en) | 2015-04-07 | 2016-10-13 | Cell Id Pte Ltd | A dc heater |
-
2016
- 2016-10-21 DE DE102016120124.3A patent/DE102016120124B8/de active Active
-
2017
- 2017-10-20 KR KR1020197014410A patent/KR20190082233A/ko not_active Application Discontinuation
- 2017-10-20 CA CA3041042A patent/CA3041042A1/en not_active Abandoned
- 2017-10-20 AU AU2017345281A patent/AU2017345281A1/en not_active Abandoned
- 2017-10-20 EP EP20174775.5A patent/EP3733292B1/de active Active
- 2017-10-20 US US16/343,675 patent/US20200063173A1/en active Pending
- 2017-10-20 ES ES17797248T patent/ES2890411T3/es active Active
- 2017-10-20 EP EP17797248.6A patent/EP3528954B1/de active Active
- 2017-10-20 WO PCT/EP2017/076902 patent/WO2018073435A1/en unknown
- 2017-10-20 JP JP2019520521A patent/JP7140759B2/ja active Active
- 2017-10-20 CN CN201780065390.2A patent/CN109862965B/zh active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102013215166B3 (de) * | 2013-08-01 | 2014-10-30 | Gna Biosolutions Gmbh | PCR-Verfahren zur Superamplifikation |
| DE102013215168A1 (de) * | 2013-08-01 | 2015-02-05 | Gna Biosolutions Gmbh | Verfahren zum Vervielfältigen von Nukleinsäuren |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3733292A1 (de) | 2020-11-04 |
| JP2020504596A (ja) | 2020-02-13 |
| CA3041042A1 (en) | 2018-04-26 |
| WO2018073435A1 (en) | 2018-04-26 |
| CN109862965A (zh) | 2019-06-07 |
| JP7140759B2 (ja) | 2022-09-21 |
| EP3733292B1 (de) | 2023-11-29 |
| CN109862965B (zh) | 2022-05-03 |
| DE102016120124A1 (de) | 2018-04-26 |
| DE102016120124A8 (de) | 2018-08-16 |
| AU2017345281A1 (en) | 2019-05-02 |
| EP3528954B1 (de) | 2021-06-23 |
| EP3528954A1 (de) | 2019-08-28 |
| DE102016120124B4 (de) | 2018-07-19 |
| US20200063173A1 (en) | 2020-02-27 |
| KR20190082233A (ko) | 2019-07-09 |
| ES2890411T3 (es) | 2022-01-19 |
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Owner name: HP HEALTH SOLUTIONS GERMANY GMBH, DE Free format text: FORMER OWNER: GNA BIOSOLUTIONS GMBH, 82152 PLANEGG, DE |
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| R082 | Change of representative |
Representative=s name: TAUTZ & SCHUHMACHER PATENTANWAELTE PARTGMBB, DE Representative=s name: TAUTZ & SCHUHMACHER PATENTANWAELTE GBR, DE |