AU2008307153B2 - Nucleic acid amplification - Google Patents

Nucleic acid amplification Download PDF

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Publication number
AU2008307153B2
AU2008307153B2 AU2008307153A AU2008307153A AU2008307153B2 AU 2008307153 B2 AU2008307153 B2 AU 2008307153B2 AU 2008307153 A AU2008307153 A AU 2008307153A AU 2008307153 A AU2008307153 A AU 2008307153A AU 2008307153 B2 AU2008307153 B2 AU 2008307153B2
Authority
AU
Australia
Prior art keywords
primer
facilitator oligonucleotide
oligonucleotide
facilitator
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2008307153A
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English (en)
Other versions
AU2008307153A1 (en
Inventor
Keith Norman Rand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
Original Assignee
Commonwealth Scientific and Industrial Research Organization CSIRO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2007905445A external-priority patent/AU2007905445A0/en
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Priority to AU2008307153A priority Critical patent/AU2008307153B2/en
Publication of AU2008307153A1 publication Critical patent/AU2008307153A1/en
Application granted granted Critical
Publication of AU2008307153B2 publication Critical patent/AU2008307153B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2008307153A 2007-10-04 2008-10-03 Nucleic acid amplification Ceased AU2008307153B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2008307153A AU2008307153B2 (en) 2007-10-04 2008-10-03 Nucleic acid amplification

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
AU2007905445A AU2007905445A0 (en) 2007-10-04 Nucleic acid amplification
AU2007905445 2007-10-04
PCT/AU2008/001475 WO2009043112A1 (en) 2007-10-04 2008-10-03 Nucleic acid amplification
AU2008307153A AU2008307153B2 (en) 2007-10-04 2008-10-03 Nucleic acid amplification

Publications (2)

Publication Number Publication Date
AU2008307153A1 AU2008307153A1 (en) 2009-04-09
AU2008307153B2 true AU2008307153B2 (en) 2015-03-12

Family

ID=40525777

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008307153A Ceased AU2008307153B2 (en) 2007-10-04 2008-10-03 Nucleic acid amplification

Country Status (7)

Country Link
US (1) US8455197B2 (enExample)
EP (1) EP2207896B1 (enExample)
JP (1) JP5535921B2 (enExample)
CN (1) CN101889096B (enExample)
AU (1) AU2008307153B2 (enExample)
ES (1) ES2460896T3 (enExample)
WO (1) WO2009043112A1 (enExample)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009223671B2 (en) * 2008-03-11 2014-11-27 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
WO2011009073A1 (en) * 2009-07-16 2011-01-20 Abbott Laboratories A method for amplification of nucleic acids
EP3660165B1 (en) 2009-12-22 2023-01-04 Sequenom, Inc. Processes and kits for identifying aneuploidy
EP2702168B1 (en) 2011-04-29 2018-01-17 Sequenom, Inc. Quantification of a minority nucleic acid species
AU2012340118A1 (en) * 2011-11-17 2014-04-24 Rheonix, Inc. System and methods for selective molecular analysis
EP3757210B1 (en) 2012-03-02 2022-08-24 Sequenom, Inc. Methods for enriching cancer nucleic acid from a biological sample
CA2872867C (en) 2012-05-11 2020-09-15 Clinical Genomics Pty Ltd Diagnostic gene marker panel for colorectal cancer
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014011928A1 (en) 2012-07-13 2014-01-16 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
CA2893817C (en) * 2012-12-13 2021-03-09 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
EP3597774A1 (en) 2013-03-13 2020-01-22 Sequenom, Inc. Primers for dna methylation analysis
EP2971134B1 (en) * 2013-03-15 2023-10-25 Aegea Biotechnologies, Inc. Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence
EP2971124B1 (en) * 2013-03-15 2019-12-25 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids utilizing clamp oligonuleotides
CN105555971B (zh) * 2013-08-09 2017-08-29 卢米耐克斯公司 用于在核酸测定中改善熔链分辨和多重性的探针
US11365447B2 (en) 2014-03-13 2022-06-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR102343605B1 (ko) * 2014-05-19 2021-12-27 윌리엄 마쉬 라이스 유니버시티 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭
CN104131070A (zh) * 2014-05-22 2014-11-05 苏州工业园区为真生物医药科技有限公司 一种对基因甲基化检测的方法
EP3209801B1 (en) 2014-10-20 2023-06-28 Commonwealth Scientific and Industrial Research Organisation Genome methylation analysis
CN105331727B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒
GB201612011D0 (en) * 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
ES3008759T3 (en) * 2016-08-02 2025-03-25 Hoffmann La Roche Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids
WO2018048957A2 (en) 2016-09-06 2018-03-15 Swift Biosciences, Inc. Normalization of ngs library concentration
DE102016120124B8 (de) * 2016-10-21 2018-08-23 Gna Biosolutions Gmbh Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens
US10787699B2 (en) * 2017-02-08 2020-09-29 Microsoft Technology Licensing, Llc Generating pluralities of primer and payload designs for retrieval of stored nucleotides
EP3530753A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
WO2019162529A1 (de) * 2018-02-26 2019-08-29 Agct Gmbh Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
EP3530754A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
CN112074612A (zh) * 2018-02-26 2020-12-11 Agct有限公司 一种具有更高特异性的核酸扩增方法
EP3759246A1 (de) * 2018-02-28 2021-01-06 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
CN114075595B (zh) * 2021-11-22 2023-11-17 上海交通大学 甲基化检测组合物、试剂盒及方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003074724A2 (en) * 2002-03-01 2003-09-12 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335184B1 (en) * 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
ATE280177T1 (de) * 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
TWI257426B (en) * 2001-08-20 2006-07-01 Takara Bio Inc Nucleic acid amplification methods
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
US20060014183A1 (en) * 2004-06-10 2006-01-19 Pfundheller Henrik M Extendable probes
CN101443458B (zh) * 2006-03-28 2014-04-02 联邦科学及工业研究组织 扩增dna片段
WO2008064687A1 (en) * 2006-11-27 2008-06-05 Fluimedix Fidelity enhanced allele specific amplification method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003074724A2 (en) * 2002-03-01 2003-09-12 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids

Also Published As

Publication number Publication date
CN101889096A (zh) 2010-11-17
EP2207896A4 (en) 2010-11-10
US8455197B2 (en) 2013-06-04
EP2207896A1 (en) 2010-07-21
AU2008307153A1 (en) 2009-04-09
EP2207896B1 (en) 2014-01-29
JP2010539956A (ja) 2010-12-24
JP5535921B2 (ja) 2014-07-02
CN101889096B (zh) 2015-10-21
WO2009043112A1 (en) 2009-04-09
ES2460896T3 (es) 2014-05-14
US20110033851A1 (en) 2011-02-10

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MK14 Patent ceased section 143(a) (annual fees not paid) or expired