ES2212305T3 - Polinucleotidos encapsulados en liposomas, composicion y procedimiento. - Google Patents
Polinucleotidos encapsulados en liposomas, composicion y procedimiento.Info
- Publication number
- ES2212305T3 ES2212305T3 ES98930447T ES98930447T ES2212305T3 ES 2212305 T3 ES2212305 T3 ES 2212305T3 ES 98930447 T ES98930447 T ES 98930447T ES 98930447 T ES98930447 T ES 98930447T ES 2212305 T3 ES2212305 T3 ES 2212305T3
- Authority
- ES
- Spain
- Prior art keywords
- lipid
- liposomes
- polynucleotide
- solvent
- cationic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 239000000203 mixture Substances 0.000 title claims abstract description 64
- 238000000034 method Methods 0.000 title claims abstract description 39
- 150000002632 lipids Chemical class 0.000 claims abstract description 126
- 239000002157 polynucleotide Substances 0.000 claims abstract description 82
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 81
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 81
- 125000002091 cationic group Chemical group 0.000 claims abstract description 60
- 230000007935 neutral effect Effects 0.000 claims abstract description 57
- 239000000725 suspension Substances 0.000 claims abstract description 20
- 108091034117 Oligonucleotide Proteins 0.000 claims description 137
- 239000002904 solvent Substances 0.000 claims description 59
- 239000002245 particle Substances 0.000 claims description 42
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- -1 cationic lipid Chemical class 0.000 claims description 29
- KSXTUUUQYQYKCR-LQDDAWAPSA-M 2,3-bis[[(z)-octadec-9-enoyl]oxy]propyl-trimethylazanium;chloride Chemical compound [Cl-].CCCCCCCC\C=C/CCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC KSXTUUUQYQYKCR-LQDDAWAPSA-M 0.000 claims description 23
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 22
- 238000005538 encapsulation Methods 0.000 claims description 21
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- 239000002202 Polyethylene glycol Substances 0.000 claims description 18
- 229920001223 polyethylene glycol Polymers 0.000 claims description 18
- 238000001727 in vivo Methods 0.000 claims description 16
- 150000003904 phospholipids Chemical class 0.000 claims description 16
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- LDGWQMRUWMSZIU-LQDDAWAPSA-M 2,3-bis[(z)-octadec-9-enoxy]propyl-trimethylazanium;chloride Chemical compound [Cl-].CCCCCCCC\C=C/CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC\C=C/CCCCCCCC LDGWQMRUWMSZIU-LQDDAWAPSA-M 0.000 claims description 6
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- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 claims description 6
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- WALUVDCNGPQPOD-UHFFFAOYSA-M 2,3-di(tetradecoxy)propyl-(2-hydroxyethyl)-dimethylazanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCOCC(C[N+](C)(C)CCO)OCCCCCCCCCCCCCC WALUVDCNGPQPOD-UHFFFAOYSA-M 0.000 claims description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 3
- HIHOWBSBBDRPDW-PTHRTHQKSA-N [(3s,8s,9s,10r,13r,14s,17r)-10,13-dimethyl-17-[(2r)-6-methylheptan-2-yl]-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1h-cyclopenta[a]phenanthren-3-yl] n-[2-(dimethylamino)ethyl]carbamate Chemical compound C1C=C2C[C@@H](OC(=O)NCCN(C)C)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HIHOWBSBBDRPDW-PTHRTHQKSA-N 0.000 claims description 3
- UMGXUWVIJIQANV-UHFFFAOYSA-M didecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC UMGXUWVIJIQANV-UHFFFAOYSA-M 0.000 claims description 3
- OGQYPPBGSLZBEG-UHFFFAOYSA-N dimethyl(dioctadecyl)azanium Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCCCCCCCCCCCCC OGQYPPBGSLZBEG-UHFFFAOYSA-N 0.000 claims description 3
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- BAQCROVBDNBEEB-UBYUBLNFSA-N Metrizamide Chemical compound CC(=O)N(C)C1=C(I)C(NC(C)=O)=C(I)C(C(=O)N[C@@H]2[C@H]([C@H](O)[C@@H](CO)OC2O)O)=C1I BAQCROVBDNBEEB-UBYUBLNFSA-N 0.000 description 17
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- A—HUMAN NECESSITIES
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- Biochemistry (AREA)
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- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
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| US5049097P | 1997-06-23 | 1997-06-23 | |
| US50490P | 1997-06-23 |
Publications (1)
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| ES2212305T3 true ES2212305T3 (es) | 2004-07-16 |
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| ES98930447T Expired - Lifetime ES2212305T3 (es) | 1997-06-23 | 1998-06-22 | Polinucleotidos encapsulados en liposomas, composicion y procedimiento. |
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| JP (1) | JP2002508765A (enExample) |
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| AU (1) | AU7983798A (enExample) |
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| ES (1) | ES2212305T3 (enExample) |
| TW (1) | TWI225412B (enExample) |
| WO (1) | WO1998058630A1 (enExample) |
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| US5855911A (en) * | 1995-08-29 | 1999-01-05 | Board Of Regents, The University Of Texas System | Liposomal phosphodiester, phosphorothioate, and P-ethoxy oligonucleotides |
| US7309692B1 (en) * | 1996-07-08 | 2007-12-18 | Board Of Regents, The University Of Texas System | Inhibition of chronic myelogenous leukemic cell growth by liposomal-antisense oligodeoxy-nucleotides targeting to GRB2 or CRK1 |
| US6977244B2 (en) | 1996-10-04 | 2005-12-20 | Board Of Regents, The University Of Texas Systems | Inhibition of Bcl-2 protein expression by liposomal antisense oligodeoxynucleotides |
| US6056973A (en) * | 1996-10-11 | 2000-05-02 | Sequus Pharmaceuticals, Inc. | Therapeutic liposome composition and method of preparation |
| US7262173B2 (en) * | 1997-03-21 | 2007-08-28 | Georgetown University | Chemosensitizing with liposomes containing oligonucleotides |
| US6126965A (en) * | 1997-03-21 | 2000-10-03 | Georgetown University School Of Medicine | Liposomes containing oligonucleotides |
| US6559129B1 (en) * | 1997-03-21 | 2003-05-06 | Georgetown University | Cationic liposomal delivery system and therapeutic use thereof |
| US20030229040A1 (en) * | 1997-03-21 | 2003-12-11 | Georgetown University | Cationic liposomal delivery system and therapeutic use thereof |
| US7285288B1 (en) | 1997-10-03 | 2007-10-23 | Board Of Regents, The University Of Texas System | Inhibition of Bcl-2 protein expression by liposomal antisense oligodeoxynucleotides |
| US7704962B1 (en) | 1997-10-03 | 2010-04-27 | Board Of Regents, The University Of Texas System | Small oligonucleotides with anti-tumor activity |
| AU773515B2 (en) * | 1999-02-08 | 2004-05-27 | Alza Corporation | Method for controlling liposome size |
| EP1365794A2 (en) * | 2000-07-21 | 2003-12-03 | Lue, Tom | Prevention and treatment of sexual arousal disorders |
| US7223406B2 (en) | 2000-07-21 | 2007-05-29 | The Regents Of The University Of California | Methods and compositions for preventing and treating male erectile dysfunction and female sexual arousal disorder |
| US20020146829A1 (en) * | 2000-11-29 | 2002-10-10 | Aventis Pharmaceuticals Products Inc. | Neutral and anionic colloidal particles for gene delivery |
| US6610322B1 (en) * | 2000-12-20 | 2003-08-26 | Brian Charles Keller | Self forming, thermodynamically stable liposomes and their applications |
| WO2002087541A1 (en) * | 2001-04-30 | 2002-11-07 | Protiva Biotherapeutics Inc. | Lipid-based formulations for gene transfer |
| KR100411234B1 (ko) * | 2001-08-14 | 2003-12-18 | 한국과학기술원 | 올리고뉴클레오티드 혼성화 미셀 및 그의 제조방법 |
| CA2471967A1 (en) * | 2002-01-03 | 2003-07-31 | Board Of Regents, The University Of Texas System | Wt1 antisense oligos for the inhibition of breast cancer |
| KR100466254B1 (ko) * | 2002-02-25 | 2005-01-14 | 한국과학기술원 | 세포내 전달을 위한 올리고뉴클레오티드와 친수성 고분자로 구성되는 유전자 전달용 접합체, 고분자 전해질 복합 미셀 및 그의 제조방법 |
| AU2003237864B2 (en) * | 2002-05-15 | 2008-12-18 | California Pacific Medical Center | Delivery of nucleic acid-like compounds |
| US20030219696A1 (en) * | 2002-05-23 | 2003-11-27 | Moreland Gerald W. | Method and apparatus for preventing backflow in dental saliva evacuators |
| WO2004002468A1 (en) * | 2002-06-26 | 2004-01-08 | Medigene Oncology Gmbh | Method of producing a cationic liposomal preparation comprising a lipophilic compound |
| US7901708B2 (en) | 2002-06-28 | 2011-03-08 | Protiva Biotherapeutics, Inc. | Liposomal apparatus and manufacturing methods |
| AU2002953285A0 (en) * | 2002-12-12 | 2003-01-02 | Protech Research Pty Ltd | Yeast treatment |
| ES2366617T3 (es) | 2003-04-21 | 2011-10-21 | Epeius Biotechnologies Corporation | Métodos y composiciones para el tratamiento de transtornos. |
| US20070178066A1 (en) | 2003-04-21 | 2007-08-02 | Hall Frederick L | Pathotropic targeted gene delivery system for cancer and other disorders |
| DK1765294T3 (da) * | 2004-05-12 | 2008-11-10 | Baxter Int | Nukleinsyremikrokugler samt deres fremstilling og afgivelse |
| EP1904111A4 (en) | 2005-06-03 | 2009-08-19 | Univ Johns Hopkins | COMPOSITIONS AND METHODS FOR REDUCING MICRORNA EXPRESSION FOR THE TREATMENT OF NEOPLASIA |
| US20070087045A1 (en) * | 2005-10-14 | 2007-04-19 | Industrial Technology Research Institute | Lipid carrier and method of preparing the same |
| WO2007080902A1 (ja) | 2006-01-11 | 2007-07-19 | Kyowa Hakko Kogyo Co., Ltd. | 眼球において標的遺伝子の発現を抑制する組成物および眼球における疾患の治療剤 |
| EP2639315A1 (en) | 2007-05-11 | 2013-09-18 | The Johns Hopkins University | Biomarkers for melanoma |
| PL208054B1 (pl) * | 2007-09-06 | 2011-03-31 | Akademia Medyczna Im Piastow Śląskich We Wrocławiu | Kompozycja lipidowa do wytwarzania lipidowego nośnika dla leków genetycznych i jej zastosowanie |
| US20110038941A1 (en) * | 2007-12-27 | 2011-02-17 | The Ohio State University Research Foundation | Lipid Nanoparticle Compositions and Methods of Making and Using the Same |
| WO2009137095A2 (en) | 2008-05-08 | 2009-11-12 | The Johns Hopkins University | Compositions and methods for modulating an immune response |
| WO2010013815A1 (ja) | 2008-08-01 | 2010-02-04 | 協和発酵キリン株式会社 | 標的遺伝子の発現を抑制する組成物 |
| EP2408916A2 (en) | 2009-03-19 | 2012-01-25 | Merck Sharp&Dohme Corp. | RNA INTERFERENCE MEDIATED INHIBITION OF CONNECTIVE TISSUE GROWTH FACTOR (CTGF) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
| US20120029054A1 (en) | 2009-03-19 | 2012-02-02 | Merck Sharp & Dohme Corp. | RNA Interference Mediated Inhibition of GATA Binding Protein 3 (GATA3) Gene Expression Using Short Intefering Nucleic Acid (siNA) |
| EP2408458A1 (en) | 2009-03-19 | 2012-01-25 | Merck Sharp&Dohme Corp. | RNA INTERFERENCE MEDIATED INHIBITION OF SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 6 (STAT6) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
| SG174452A1 (en) | 2009-03-19 | 2011-10-28 | Merck Sharp & Dohme | RNA INTERFERENCE MEDIATED INHIBITION OF BTB AND CNC HOMOLOGY 1, BASIC LEUCINE ZIPPER TRANSCRIPTION FACTOR 1 (BACH 1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) SEQUENCE LISTING |
| CN102439152A (zh) | 2009-03-27 | 2012-05-02 | 默沙东公司 | 使用短干扰核酸（siNA）的RNA干扰介导的细胞间粘附分子1（ICAM-1）基因表达的抑制 |
| WO2010111471A2 (en) | 2009-03-27 | 2010-09-30 | Merck Sharp & Dohme Corp. | RNA INTERFERENCE MEDIATED INHIBITION OF SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 1 (STAT1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
| WO2010111468A2 (en) | 2009-03-27 | 2010-09-30 | Merck Sharp & Dohme Corp. | RNA INTERFERENCE MEDIATED INHIBITION OF THE NERVE GROWTH FACTOR BETA CHAIN (NGFß) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (SINA) |
| US20120022143A1 (en) | 2009-03-27 | 2012-01-26 | Merck Sharp & Dohme Corp | RNA Interference Mediated Inhibition of the Thymic Stromal Lymphopoietin (TSLP) Gene Expression Using Short Interfering Nucliec Acid (siNA) |
| US20120010272A1 (en) | 2009-03-27 | 2012-01-12 | Merck Sharp & Dohme Corp. | RNA Interference Mediated Inhibition of Apoptosis Signal-Regulating Kinase 1 (ASK1) Gene Expression Using Short Interfering Nucleic Acid (siNA) |
| KR101689787B1 (ko) | 2009-08-31 | 2016-12-26 | 나노캐리어 가부시키가이샤 | 입자 조성물 및 이를 가진 의약 조성물 |
| JP4653242B1 (ja) | 2010-02-12 | 2011-03-16 | ナノキャリア株式会社 | 粒子状医薬組成物 |
| WO2011119995A2 (en) | 2010-03-26 | 2011-09-29 | Cerulean Pharma Inc. | Formulations and methods of use |
| US9408914B2 (en) | 2010-04-28 | 2016-08-09 | Kyowa Hakko Kirin Co., Ltd. | Cationic lipid |
| EP2567952A4 (en) | 2010-04-28 | 2015-11-25 | Kyowa Hakko Kirin Co Ltd | CATIONIC LIPID |
| CA2799403C (en) | 2010-05-14 | 2020-01-21 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for treating leukemia |
| CN103119160B (zh) | 2010-05-14 | 2016-06-01 | 达那-法伯癌症研究所 | 用于调节代谢的组合物和方法 |
| US8518907B2 (en) | 2010-08-02 | 2013-08-27 | Merck Sharp & Dohme Corp. | RNA interference mediated inhibition of catenin (cadherin-associated protein), beta 1 (CTNNB1) gene expression using short interfering nucleic acid (siNA) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4394448A (en) * | 1978-02-24 | 1983-07-19 | Szoka Jr Francis C | Method of inserting DNA into living cells |
| DE69634084T2 (de) * | 1995-06-07 | 2005-12-08 | Inex Pharmaceuticals Corp. | Herstellung von lipid-nukleinsäure partikeln duch ein hydrophobische lipid-nukleinsäuree komplexe zwischenprodukt und zur verwendung in der gentransfer |
| US5705385A (en) * | 1995-06-07 | 1998-01-06 | Inex Pharmaceuticals Corporation | Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer |
| WO1997004748A2 (en) * | 1995-08-01 | 1997-02-13 | Advanced Therapies, Inc. | Enhanced artificial viral envelopes for cellular delivery of therapeutic substances |
| US5855911A (en) * | 1995-08-29 | 1999-01-05 | Board Of Regents, The University Of Texas System | Liposomal phosphodiester, phosphorothioate, and P-ethoxy oligonucleotides |
| FR2751222B1 (fr) * | 1996-07-16 | 1998-10-09 | Capsulis | Compositions contenant au moins un acide nucleique et leurs applications dans le domaine biomedical, en particulier en therapie genique |
-
1998
- 1998-06-22 DE DE69820465T patent/DE69820465T2/de not_active Expired - Fee Related
- 1998-06-22 AT AT98930447T patent/ATE255882T1/de not_active IP Right Cessation
- 1998-06-22 CA CA002294579A patent/CA2294579C/en not_active Expired - Fee Related
- 1998-06-22 JP JP50493699A patent/JP2002508765A/ja not_active Abandoned
- 1998-06-22 AU AU79837/98A patent/AU7983798A/en not_active Abandoned
- 1998-06-22 WO PCT/US1998/012937 patent/WO1998058630A1/en not_active Ceased
- 1998-06-22 DK DK98930447T patent/DK1017365T3/da active
- 1998-06-22 ES ES98930447T patent/ES2212305T3/es not_active Expired - Lifetime
- 1998-06-22 EP EP98930447A patent/EP1017365B1/en not_active Expired - Lifetime
- 1998-06-22 TW TW087109984A patent/TWI225412B/zh not_active IP Right Cessation
- 1998-06-23 US US09/103,341 patent/US6120798A/en not_active Expired - Lifetime
Also Published As
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|---|---|
| US6120798A (en) | 2000-09-19 |
| WO1998058630A1 (en) | 1998-12-30 |
| JP2002508765A (ja) | 2002-03-19 |
| DE69820465T2 (de) | 2004-09-16 |
| AU7983798A (en) | 1999-01-04 |
| EP1017365A1 (en) | 2000-07-12 |
| ATE255882T1 (de) | 2003-12-15 |
| EP1017365B1 (en) | 2003-12-10 |
| CA2294579C (en) | 2007-10-09 |
| TWI225412B (en) | 2004-12-21 |
| HK1025906A1 (en) | 2000-12-01 |
| DE69820465D1 (de) | 2004-01-22 |
| DK1017365T3 (da) | 2004-03-29 |
| CA2294579A1 (en) | 1998-12-30 |
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