KR100411234B1 - 올리고뉴클레오티드 혼성화 미셀 및 그의 제조방법 - Google Patents
올리고뉴클레오티드 혼성화 미셀 및 그의 제조방법 Download PDFInfo
- Publication number
- KR100411234B1 KR100411234B1 KR10-2001-0048880A KR20010048880A KR100411234B1 KR 100411234 B1 KR100411234 B1 KR 100411234B1 KR 20010048880 A KR20010048880 A KR 20010048880A KR 100411234 B1 KR100411234 B1 KR 100411234B1
- Authority
- KR
- South Korea
- Prior art keywords
- oligonucleotide
- micelle
- micelles
- hybridized
- preparing
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6905—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a colloid or an emulsion
- A61K47/6907—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a colloid or an emulsion the form being a microemulsion, nanoemulsion or micelle
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/549—Sugars, nucleosides, nucleotides or nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
- A61K47/593—Polyesters, e.g. PLGA or polylactide-co-glycolide
Abstract
Description
Claims (5)
- (ⅰ) 지방족 폴리에스테르계 생분해성 고분자 및 활성화제를 유기용매하에 반응시켜서 고분자를 활성화시키고, 건조시키는 공정;(ⅱ) 전기 활성화된 고분자와 5'-말단에 아민기를 포함하는 올리고뉴클레오티드를 2:1 내지 1:2의 몰비로 혼합하고, 유기용매에서 반응시켜서 접합체를 작제하는 공정; 및,(ⅲ) 전기 접합체를 증류수에 분산시켜서 미셀을 형성하는 공정을 포함하는 올리고뉴클레오티드 혼성화 미셀의 제조방법.
- 제 1항에 있어서,지방족 폴리에스테르계 생분해성 고분자는 폴리락트산(PLA), 폴리글리콜산(PGA), 폴리(D,L-락트산-co-글리콜산, PLGA), 폴리카프로락톤, 폴리발레로락톤, 폴리하이드록시 부티레이트, 폴리하이드록시 발러레이트 또는 이들의 혼합물인 것을 특징으로 하는올리고뉴클레오티드 혼성화 미셀의 제조방법.
- 제 1항에 있어서,활성화제는 디클로로헥실카보디이미드(DCC), p-나이트로페닐클로로포르메이트(p-nitrophenylchloroformate), 카보닐디이미다졸(CDI),N,N′-디숙신이미딜 카보네이트(N,N′-disuccinimidyl carbonate),N-하이드록실숙신이미드(NHS) 또는 이들의 혼합물인 것을 특징으로하는올리고뉴클레오티드 혼성화 미셀의 제조방법.
- 제 1항에 있어서,유기용매는 디메틸설폭사이드(dimethylsulfoxie, DMSO)인 것을특징으로 하는올리고뉴클레오티드 혼성화 미셀의 제조방법.
- 제 1항의 방법으로 제조되어, 5'-말단 인산기에 아민기(-NH2)를 포함하는 올리고뉴클레오티드와 지방족 폴리에스테르계 생분해성 고분자를 포함하는 올리고뉴클레오티드 혼성화 미셀.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2001-0048880A KR100411234B1 (ko) | 2001-08-14 | 2001-08-14 | 올리고뉴클레오티드 혼성화 미셀 및 그의 제조방법 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2001-0048880A KR100411234B1 (ko) | 2001-08-14 | 2001-08-14 | 올리고뉴클레오티드 혼성화 미셀 및 그의 제조방법 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20030014958A KR20030014958A (ko) | 2003-02-20 |
KR100411234B1 true KR100411234B1 (ko) | 2003-12-18 |
Family
ID=27719109
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR10-2001-0048880A KR100411234B1 (ko) | 2001-08-14 | 2001-08-14 | 올리고뉴클레오티드 혼성화 미셀 및 그의 제조방법 |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR100411234B1 (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010131835A3 (ko) * | 2009-05-14 | 2011-01-20 | 한국과학기술연구원 | 작은 올리고뉴클레오티드의 중합 방법 및 상기 중합 방법에 의하여 제조된 고분자 올리고뉴클레오티드의 용도 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100449889B1 (ko) * | 2001-08-30 | 2004-09-22 | 동국제약 주식회사 | 음이온 고분자와 인지질의 결합체가 함유된 인지질 리포좀및 그의 제조방법과 응용 |
KR100466254B1 (ko) * | 2002-02-25 | 2005-01-14 | 한국과학기술원 | 세포내 전달을 위한 올리고뉴클레오티드와 친수성 고분자로 구성되는 유전자 전달용 접합체, 고분자 전해질 복합 미셀 및 그의 제조방법 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20000010780A (ko) * | 1996-05-08 | 2000-02-25 | 와이에키 피아세키 | 유전자 물질을 전달용 양이온 비로좀 |
US6120798A (en) * | 1997-06-23 | 2000-09-19 | Alza Corporation | Liposome-entrapped polynucleotide composition and method |
US6126965A (en) * | 1997-03-21 | 2000-10-03 | Georgetown University School Of Medicine | Liposomes containing oligonucleotides |
US6265389B1 (en) * | 1995-08-31 | 2001-07-24 | Alkermes Controlled Therapeutics, Inc. | Microencapsulation and sustained release of oligonucleotides |
US6270795B1 (en) * | 1995-11-09 | 2001-08-07 | Microbiological Research Authority | Method of making microencapsulated DNA for vaccination and gene therapy |
-
2001
- 2001-08-14 KR KR10-2001-0048880A patent/KR100411234B1/ko active IP Right Grant
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6265389B1 (en) * | 1995-08-31 | 2001-07-24 | Alkermes Controlled Therapeutics, Inc. | Microencapsulation and sustained release of oligonucleotides |
US6270795B1 (en) * | 1995-11-09 | 2001-08-07 | Microbiological Research Authority | Method of making microencapsulated DNA for vaccination and gene therapy |
KR20000010780A (ko) * | 1996-05-08 | 2000-02-25 | 와이에키 피아세키 | 유전자 물질을 전달용 양이온 비로좀 |
US6126965A (en) * | 1997-03-21 | 2000-10-03 | Georgetown University School Of Medicine | Liposomes containing oligonucleotides |
US6120798A (en) * | 1997-06-23 | 2000-09-19 | Alza Corporation | Liposome-entrapped polynucleotide composition and method |
Non-Patent Citations (1)
Title |
---|
Akhtar et al., vol22, pp47-56, 1992 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010131835A3 (ko) * | 2009-05-14 | 2011-01-20 | 한국과학기술연구원 | 작은 올리고뉴클레오티드의 중합 방법 및 상기 중합 방법에 의하여 제조된 고분자 올리고뉴클레오티드의 용도 |
CN102361882A (zh) * | 2009-05-14 | 2012-02-22 | 韩国科学技术研究院 | 短寡核苷酸的聚合方法以及通过该聚合方法制备的高分子寡核苷酸的应用 |
Also Published As
Publication number | Publication date |
---|---|
KR20030014958A (ko) | 2003-02-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9567430B2 (en) | Enzymatic synthesis of poly(amine-co-esters) and methods of use thereof for gene delivery | |
US8324365B2 (en) | Conjugate for gene transfer comprising oligonucleotide and hydrophilic polymer, polyelectrolyte complex micelles formed from the conjugate, and methods for preparation thereof | |
KR101564796B1 (ko) | 비하전성 친수성 블록 및 측사슬의 일부에 소수성 기가 도입된 카티온성 폴리아미노산 블록을 포함하여 이루어지는 공중합체, 그 사용 | |
Chirila et al. | The use of synthetic polymers for delivery of therapeutic antisense oligodeoxynucleotides | |
CA2717778C (fr) | Polymere derive de la polyethylenimine lineaire pour le transfert de gene | |
CN1646174B (zh) | 可控降解的聚合生物分子或药物载体及其合成方法 | |
CN101952434B (zh) | 使用合成的聚合物递送对基因沉默有活性的核酸的工具 | |
US20110008451A1 (en) | Compositions and methods for controlled delivery of inhibitory ribonucleic acids | |
US11814464B2 (en) | Poly(amine-co-ester) polymers and polyplexes with modified end groups and methods of use thereof | |
Li et al. | Sustained delivery and expression of plasmid DNA based on biodegradable polyester, poly (D, L-lactide-co-4-hydroxy-L-proline) | |
US20230233693A1 (en) | Poly(amine-co-ester) polymers with modified end groups and enhanced pulmonary delivery | |
WO2010131777A1 (ja) | 結晶性ポリオール微粒子及びその調製方法 | |
KR100411234B1 (ko) | 올리고뉴클레오티드 혼성화 미셀 및 그의 제조방법 | |
CA2669846C (en) | Materials and methods for introducing genetic material into living cells | |
KR100466254B1 (ko) | 세포내 전달을 위한 올리고뉴클레오티드와 친수성 고분자로 구성되는 유전자 전달용 접합체, 고분자 전해질 복합 미셀 및 그의 제조방법 | |
Zhang et al. | Calcium phosphate/DNA co‐precipitates encapsulated fast‐degrading polymer films for substrate‐mediated gene delivery | |
JP2011010549A (ja) | ポリエチレングリコールの結合した核酸のコンジュゲートとリン酸カルシウムの有機−無機ハイブリッド型ナノ粒子 | |
US20230172870A1 (en) | Surface conjugation to poly(amine-co-ester) nanoparticles for targeting to cells and tissues | |
Semwal et al. | Drug delivery systems: selection criteria and use | |
Kushwaha et al. | Utilization of Silk for Gene Delivery | |
Kiatwuthinon | Synthetic Dna Nanomaterials For Drug Delivery And 3D Cell Culture |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20121011 Year of fee payment: 10 |
|
FPAY | Annual fee payment |
Payment date: 20130904 Year of fee payment: 11 |
|
FPAY | Annual fee payment |
Payment date: 20140917 Year of fee payment: 12 |
|
FPAY | Annual fee payment |
Payment date: 20150909 Year of fee payment: 13 |
|
FPAY | Annual fee payment |
Payment date: 20160912 Year of fee payment: 14 |
|
FPAY | Annual fee payment |
Payment date: 20170926 Year of fee payment: 15 |
|
FPAY | Annual fee payment |
Payment date: 20190909 Year of fee payment: 17 |