ES2194997T3 - Procedimiento enzimatico para el desmucilaginado de aceites vegetales mediante fosfolipasa de aspergillus. - Google Patents
Procedimiento enzimatico para el desmucilaginado de aceites vegetales mediante fosfolipasa de aspergillus.Info
- Publication number
- ES2194997T3 ES2194997T3 ES96921886T ES96921886T ES2194997T3 ES 2194997 T3 ES2194997 T3 ES 2194997T3 ES 96921886 T ES96921886 T ES 96921886T ES 96921886 T ES96921886 T ES 96921886T ES 2194997 T3 ES2194997 T3 ES 2194997T3
- Authority
- ES
- Spain
- Prior art keywords
- vegetable oils
- procedure
- demucilagination
- phospholipase
- aspergillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/913—Aspergillus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/913—Aspergillus
- Y10S435/917—Aspergillus niger
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/913—Aspergillus
- Y10S435/918—Aspergillus oryzae
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
- Enzymes And Modification Thereof (AREA)
- Edible Oils And Fats (AREA)
Abstract
LA INVENCION TRATA DE UNA ETAPA DEL PROCEDIMIENTO DE DESENGOMADO EN LA REDUCCION DE ACEITES COMESTIBLES, EN EL CUAL ACEITES VEGETALES, A LOS CUALES SE HA ELIMINADO DE MANERA PREFERENTE LOS FOSFATIDOS HIDRATABLES MEDIANTE UN PROCEDIMIENTO PREVIO ACUOSO DE DESENGOMADO, SE LIBERAN DE FOSFATIDOS NO HIDRATABLES MEDIANTE UN TRATAMIENTO ENZIMATICO, DE TAL MANERA QUE PUEDAN SER REFINADOS FISICAMENTE. LA PRINCIPAL CARACTERISTICA DE LA INVENCION ES LA UTILIZACION DE FOSFOLIPASA PROCEDENTE DE UNA CEPA DE ASPERGILLUS. EL PROCEDIMIENTO ES CUIDADOSO, ECONOMICO Y ECOLOGICO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19527274A DE19527274A1 (de) | 1995-07-26 | 1995-07-26 | Enzymatisches Verfahren zur Entschleimung von pflanzlichen Ölen mit Aspergillus-Phospholipase |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2194997T3 true ES2194997T3 (es) | 2003-12-01 |
Family
ID=7767819
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96921886T Expired - Lifetime ES2194997T3 (es) | 1995-07-26 | 1996-07-04 | Procedimiento enzimatico para el desmucilaginado de aceites vegetales mediante fosfolipasa de aspergillus. |
Country Status (18)
Country | Link |
---|---|
US (1) | US6001640A (es) |
EP (1) | EP0906379B1 (es) |
JP (1) | JPH11510195A (es) |
CN (1) | CN1074455C (es) |
AR (1) | AR003052A1 (es) |
AT (1) | ATE238406T1 (es) |
BR (1) | BR9609816A (es) |
CA (1) | CA2227568C (es) |
CZ (1) | CZ298366B6 (es) |
DE (2) | DE19527274A1 (es) |
DK (1) | DK0906379T3 (es) |
ES (1) | ES2194997T3 (es) |
HK (1) | HK1015819A1 (es) |
HU (1) | HU227760B1 (es) |
MX (1) | MX9800547A (es) |
PL (1) | PL185718B1 (es) |
TR (1) | TR199800066T1 (es) |
WO (1) | WO1997005219A1 (es) |
Families Citing this family (68)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
DE69716711T3 (de) * | 1996-12-09 | 2010-06-10 | Novozymes A/S | Verringerung von phosphorhaltigen Substanzen in Speiseölen mit hohem Anteil an nicht-hydratisierbarem Phosphor unter Verwendung einer Phospholipase, eine Phospholipase aus fädenförmigen Pilz, welche eine Phospholipase A und/oder B-Aktivität aufweist |
US6103505A (en) * | 1996-12-09 | 2000-08-15 | Novo Nordisk A/S | Method for reducing phosphorus content of edible oils |
DE19701348A1 (de) * | 1997-01-16 | 1998-07-23 | Roehm Gmbh | Protein mit Phospholipaseaktivität |
EP0973399B1 (en) | 1997-04-09 | 2002-07-17 | Danisco A/S | Improved method for preparing flour doughs and products made from such doughs using glycerol oxidase |
ES2188190T5 (es) | 1998-07-21 | 2007-11-16 | Danisco A/S | Producto alimentario. |
NZ528260A (en) | 2001-05-18 | 2005-09-30 | Danisco | Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride |
DK1443825T3 (da) * | 2001-11-06 | 2009-02-02 | Novozymes North America Inc | Modificerede valleproteinsammensætninger, der har forbedrede skumningsegenskaber |
US7943360B2 (en) | 2002-04-19 | 2011-05-17 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
US7226771B2 (en) * | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
CA2481411C (en) | 2002-04-19 | 2016-06-14 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
WO2003102118A2 (en) * | 2002-05-30 | 2003-12-11 | Council Of Scientific And Industrial Research | Process for the pre-treatment of vegetable oils for physical refining |
US20050196766A1 (en) | 2003-12-24 | 2005-09-08 | Soe Jorn B. | Proteins |
US7955814B2 (en) | 2003-01-17 | 2011-06-07 | Danisco A/S | Method |
MXPA05007653A (es) | 2003-01-17 | 2005-09-30 | Danisco | Metodo. |
DK2853593T3 (en) | 2003-03-07 | 2018-01-08 | Dsm Ip Assets Bv | Hydrolases, nucleic acids encoding them, and processes for their preparation and use |
EP1711587B1 (en) * | 2003-12-19 | 2013-04-10 | Bunge Oils, Inc | Process for improving enzymatic degumming of vegetable oils and reducing fouling of downstream processing equipment |
US7906307B2 (en) | 2003-12-24 | 2011-03-15 | Danisco A/S | Variant lipid acyltransferases and methods of making |
GB0716126D0 (en) | 2007-08-17 | 2007-09-26 | Danisco | Process |
US7718408B2 (en) | 2003-12-24 | 2010-05-18 | Danisco A/S | Method |
GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
CN103173282A (zh) | 2004-06-16 | 2013-06-26 | 帝斯曼知识产权资产管理有限公司 | 对叶绿素进行酶促脱色的组合物和方法 |
US20080070291A1 (en) * | 2004-06-16 | 2008-03-20 | David Lam | Compositions and Methods for Enzymatic Decolorization of Chlorophyll |
WO2006008508A1 (en) | 2004-07-16 | 2006-01-26 | Danisco A/S | Enzymatic oil-degumming method |
WO2006031699A2 (en) | 2004-09-10 | 2006-03-23 | Diversa Corporation | Compositions and methods for making and modifying oils |
US20070148311A1 (en) * | 2005-12-22 | 2007-06-28 | Bunge Oils, Inc. | Phytosterol esterification product and method of make same |
WO2007092314A2 (en) | 2006-02-02 | 2007-08-16 | Verenium Corporation | Esterases and related nucleic acids and methods |
CN100441668C (zh) * | 2006-06-09 | 2008-12-10 | 中国农业科学院油料作物研究所 | 超声波强化生物酶油脂脱胶的方法 |
MX2009003034A (es) | 2006-09-21 | 2009-11-18 | Verenium Corp | Fosfolipasas, acidos nucleicos que las codifican, y metodos de hacerlas y usarlas. |
BRPI0720801A2 (pt) | 2007-01-25 | 2014-09-16 | Dupont Nutrition Biosci Aps | Produção de um lipídio aciltranfersase a partir de células transformadas de bacillus licheniformis. |
US8956853B2 (en) * | 2007-01-30 | 2015-02-17 | Bunge Oils, Inc. | Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases |
US8460905B2 (en) * | 2007-09-11 | 2013-06-11 | Bunge Oils, Inc. | Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases with reduced reaction time |
CA2686161A1 (en) * | 2007-05-04 | 2008-11-13 | Akermin, Inc. | Immobilized enzymes and uses thereof |
US8076123B2 (en) * | 2007-10-26 | 2011-12-13 | Oilseeds Biorefinery Corporation | Emulsification-free degumming of oil |
US8241876B2 (en) | 2008-01-07 | 2012-08-14 | Bunge Oils, Inc. | Generation of triacylglycerols from gums |
US8911808B2 (en) | 2008-06-23 | 2014-12-16 | Cavitation Technologies, Inc. | Method for cavitation-assisted refining, degumming and dewaxing of oil and fat |
GB0904787D0 (en) * | 2009-03-20 | 2009-05-06 | Desmet Ballestra Engineering Sa | Improved enzymatic oil recuperation process |
US11247964B2 (en) | 2009-05-01 | 2022-02-15 | Rrip, Llc | Method of processing phospholipid based lipid materials |
AR077042A1 (es) | 2009-06-12 | 2011-07-27 | Danisco | Metodo para tratar una composicion que contiene pirofiofitina |
UA109884C2 (uk) | 2009-10-16 | 2015-10-26 | Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування | |
UA111708C2 (uk) | 2009-10-16 | 2016-06-10 | Бандж Ойлз, Інк. | Спосіб рафінування олії |
EA201290903A1 (ru) | 2010-03-12 | 2013-06-28 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Способ рафинирования растительного масла и рафинированное растительное масло, полученное указанным способом |
CA2798152A1 (en) | 2010-06-17 | 2011-12-22 | Dupont Nutrition Biosciences Aps | Process |
DE102010055159A1 (de) * | 2010-12-18 | 2012-06-21 | Lurgi Gmbh | Verfahren zur enzymatischen Reinigung von Ölen pflanzlicher oder tierischer Herkunft |
WO2012114232A1 (en) | 2011-02-23 | 2012-08-30 | Dupont Nutrition Biosciences Aps | Process |
CA3004088A1 (en) | 2011-02-23 | 2012-08-30 | Dupont Nutrition Biosciences Aps | Enzymatic treatment of plant oil |
CN102586011B (zh) * | 2011-12-01 | 2013-10-30 | 华中农业大学 | 一种利用磷脂酶a2进行植物油脂脱胶的方法 |
WO2013104660A1 (en) | 2012-01-13 | 2013-07-18 | Dupont Nutrition Biosciences Aps | Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation |
WO2013104659A2 (en) | 2012-01-13 | 2013-07-18 | Dupont Nutrition Biosciences Aps | Process |
WO2013160374A1 (en) | 2012-04-27 | 2013-10-31 | Dupont Nutrition Biosciences Aps | Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling |
WO2013160372A1 (en) | 2012-04-27 | 2013-10-31 | Dupont Nutrition Biosciences Aps | Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme |
CN104487558B (zh) | 2012-06-14 | 2018-07-17 | 邦吉全球创新有限责任公司 | 用于生产低饱和油的方法 |
US10294463B2 (en) | 2013-07-03 | 2019-05-21 | Keclon Sa | Modified Bacillus cereus phospholipase C protein and method of processing vegetable oil |
CN106103704A (zh) | 2014-03-19 | 2016-11-09 | 诺维信公司 | 具有磷脂酶c活性的多肽和编码这些多肽的多核苷酸 |
EP3126509A1 (en) | 2014-04-01 | 2017-02-08 | DuPont Nutrition Biosciences ApS | Method for increasing crude palm oil yields |
WO2015173426A1 (en) | 2014-05-15 | 2015-11-19 | Novozymes A/S | Compositions comprising polypeptides having phospholipase c activity and use thereof |
US9453180B2 (en) | 2014-10-15 | 2016-09-27 | Arisdyne Systems, Inc. | Process for degumming oils |
US9290717B1 (en) | 2014-12-15 | 2016-03-22 | Arisdyne Systems, Inc. | Reactor for degumming |
BR112017013040A2 (pt) | 2014-12-19 | 2018-01-09 | Dupont Nutrition Biosci Aps | método para aprimorar rendimentos de óleo de palma a partir de fruto de palma ou líquido de fruto de palma |
US9340749B1 (en) | 2015-05-06 | 2016-05-17 | Arisdyne Systems, Inc. | Method for degumming triglyceride oils |
BR112018015528B1 (pt) * | 2016-02-03 | 2022-03-15 | Fuji Oil Holdings Inc | Método para produzir manteiga de cacau, método para obter resistência a fotodegradação, alimento semelhante a chocolate, e método para produzir o mesmo |
WO2018186734A1 (en) * | 2017-04-06 | 2018-10-11 | Purac Biochem B.V. | Enzymatic degumming of unrefined triglyceride oil |
WO2018217270A1 (en) | 2017-05-24 | 2018-11-29 | Arisdyne Systems, Inc. | Oil degumming systems |
EP3728531A1 (en) * | 2017-12-21 | 2020-10-28 | Purac Biochem B.V. | Enzymatic degumming of unrefined triglyceride oil |
UA126992C2 (uk) | 2018-05-07 | 2023-03-01 | Дсм Айпі Асетс Б.В. | Спосіб ферментативного дегумування олії |
CN109989115B (zh) * | 2019-04-28 | 2021-09-03 | 四川玉竹麻业有限公司 | 一种苎麻冷轧堆氧漂脱胶工艺 |
CN110951621B (zh) * | 2019-11-18 | 2022-05-31 | 无锡优普克生物科技有限公司 | 一种产磷脂酶的黑曲霉菌株 |
WO2023108233A1 (pt) * | 2021-12-16 | 2023-06-22 | Oliveira Jean Paulo De | Processo de reaproveitamento da lisogoma aplicado no pré-tratamento de óleos vegetais degomados para posterior tratamento enzimático e transesterificação do biodiesel |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61289884A (ja) * | 1985-06-14 | 1986-12-19 | Asahi Denka Kogyo Kk | リパ−ゼ類の製造方法 |
GB8525012D0 (en) * | 1985-10-10 | 1985-11-13 | Cpc International Inc | Carbohydrate refining process |
US5288619A (en) * | 1989-12-18 | 1994-02-22 | Kraft General Foods, Inc. | Enzymatic method for preparing transesterified oils |
JPH04356191A (ja) * | 1990-07-10 | 1992-12-09 | Kanegafuchi Chem Ind Co Ltd | リン脂質の製造法 |
JPH04356192A (ja) * | 1990-07-10 | 1992-12-09 | Kanegafuchi Chem Ind Co Ltd | リン脂質の製造方法 |
DE4115938A1 (de) * | 1991-05-16 | 1992-11-19 | Metallgesellschaft Ag | Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen |
EP0546215B1 (en) * | 1991-12-12 | 1998-04-01 | Societe Des Produits Nestle S.A. | Heat stable oil-in-water emulsion and process for its preparation |
DE69332217T3 (de) * | 1992-06-16 | 2009-01-15 | Sankyo Lifetech Co.Ltd. | Phospholipase A1, Verfahren zu seiner Herstellung und Anwendung |
JP2937746B2 (ja) * | 1993-04-25 | 1999-08-23 | 昭和産業株式会社 | 油脂の精製方法 |
JP2657887B2 (ja) * | 1993-05-24 | 1997-09-30 | 日清製油株式会社 | 固定化酵素の調製方法 |
-
1995
- 1995-07-26 DE DE19527274A patent/DE19527274A1/de not_active Ceased
-
1996
- 1996-07-04 BR BR9609816A patent/BR9609816A/pt not_active Application Discontinuation
- 1996-07-04 CZ CZ0022498A patent/CZ298366B6/cs not_active IP Right Cessation
- 1996-07-04 DE DE59610388T patent/DE59610388D1/de not_active Expired - Lifetime
- 1996-07-04 US US08/983,324 patent/US6001640A/en not_active Expired - Lifetime
- 1996-07-04 ES ES96921886T patent/ES2194997T3/es not_active Expired - Lifetime
- 1996-07-04 CN CN96195847A patent/CN1074455C/zh not_active Expired - Fee Related
- 1996-07-04 TR TR1998/00066T patent/TR199800066T1/xx unknown
- 1996-07-04 PL PL96324648A patent/PL185718B1/pl not_active IP Right Cessation
- 1996-07-04 EP EP96921886A patent/EP0906379B1/de not_active Expired - Lifetime
- 1996-07-04 CA CA002227568A patent/CA2227568C/en not_active Expired - Fee Related
- 1996-07-04 DK DK96921886T patent/DK0906379T3/da active
- 1996-07-04 AT AT96921886T patent/ATE238406T1/de active
- 1996-07-04 HU HU9802813A patent/HU227760B1/hu not_active IP Right Cessation
- 1996-07-04 JP JP9507073A patent/JPH11510195A/ja active Pending
- 1996-07-04 WO PCT/DE1996/001190 patent/WO1997005219A1/de active IP Right Grant
- 1996-07-26 AR ARP960103765A patent/AR003052A1/es unknown
-
1998
- 1998-01-19 MX MX9800547A patent/MX9800547A/es unknown
-
1999
- 1999-03-01 HK HK99100813A patent/HK1015819A1/xx unknown
Also Published As
Publication number | Publication date |
---|---|
HUP9802813A3 (en) | 2000-03-28 |
CN1192231A (zh) | 1998-09-02 |
DE19527274A1 (de) | 1997-01-30 |
HK1015819A1 (en) | 1999-10-22 |
DE59610388D1 (de) | 2003-05-28 |
HUP9802813A2 (hu) | 1999-03-29 |
CA2227568C (en) | 2006-05-30 |
EP0906379B1 (de) | 2003-04-23 |
CZ298366B6 (cs) | 2007-09-12 |
US6001640A (en) | 1999-12-14 |
HU227760B1 (en) | 2012-02-28 |
DK0906379T3 (da) | 2003-08-11 |
BR9609816A (pt) | 1999-07-06 |
PL324648A1 (en) | 1998-06-08 |
CN1074455C (zh) | 2001-11-07 |
PL185718B1 (pl) | 2003-07-31 |
CA2227568A1 (en) | 1997-02-13 |
ATE238406T1 (de) | 2003-05-15 |
MX9800547A (es) | 1998-11-30 |
WO1997005219A1 (de) | 1997-02-13 |
TR199800066T1 (xx) | 1998-04-21 |
CZ22498A3 (cs) | 1998-06-17 |
EP0906379A1 (de) | 1999-04-07 |
JPH11510195A (ja) | 1999-09-07 |
AR003052A1 (es) | 1998-05-27 |
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