WO2023108233A1 - Processo de reaproveitamento da lisogoma aplicado no pré-tratamento de óleos vegetais degomados para posterior tratamento enzimático e transesterificação do biodiesel - Google Patents
Processo de reaproveitamento da lisogoma aplicado no pré-tratamento de óleos vegetais degomados para posterior tratamento enzimático e transesterificação do biodiesel Download PDFInfo
- Publication number
- WO2023108233A1 WO2023108233A1 PCT/BR2021/050555 BR2021050555W WO2023108233A1 WO 2023108233 A1 WO2023108233 A1 WO 2023108233A1 BR 2021050555 W BR2021050555 W BR 2021050555W WO 2023108233 A1 WO2023108233 A1 WO 2023108233A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- treatment
- lysogum
- oil
- degummed
- phosphorus
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 47
- 230000008569 process Effects 0.000 title claims abstract description 42
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 25
- 239000003225 biodiesel Substances 0.000 title claims abstract description 18
- 238000005809 transesterification reaction Methods 0.000 title claims abstract description 18
- 239000010773 plant oil Substances 0.000 title 1
- 239000011574 phosphorus Substances 0.000 claims abstract description 25
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 25
- 239000011575 calcium Substances 0.000 claims abstract description 24
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 24
- 239000011777 magnesium Substances 0.000 claims abstract description 24
- 229910052749 magnesium Inorganic materials 0.000 claims abstract description 24
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 23
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims abstract description 23
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 23
- 102000004190 Enzymes Human genes 0.000 claims abstract description 20
- 108090000790 Enzymes Proteins 0.000 claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 claims abstract description 11
- 239000003921 oil Substances 0.000 claims description 50
- 235000019198 oils Nutrition 0.000 claims description 50
- 238000002203 pretreatment Methods 0.000 claims description 18
- 102000015439 Phospholipases Human genes 0.000 claims description 15
- 108010064785 Phospholipases Proteins 0.000 claims description 15
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 12
- 239000008158 vegetable oil Substances 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 4
- 230000007062 hydrolysis Effects 0.000 claims description 4
- 230000004001 molecular interaction Effects 0.000 claims description 4
- 239000000470 constituent Substances 0.000 claims description 2
- 238000010908 decantation Methods 0.000 claims description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 claims 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims 1
- 239000006227 byproduct Substances 0.000 abstract description 3
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 10
- 230000009467 reduction Effects 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 238000007670 refining Methods 0.000 description 6
- 235000014113 dietary fatty acids Nutrition 0.000 description 4
- 229930195729 fatty acid Natural products 0.000 description 4
- 239000000194 fatty acid Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 229910001868 water Inorganic materials 0.000 description 4
- 150000004665 fatty acids Chemical class 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 150000003904 phospholipids Chemical class 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 2
- 238000005886 esterification reaction Methods 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 102000000571 Phospholipases A Human genes 0.000 description 1
- 108010002176 Phospholipases A Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 125000003158 alcohol group Chemical group 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 125000005313 fatty acid group Chemical group 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- This invention describes a process for reuse of lysogum, which is a residue from the enzymatic treatment PLA (Phospholipase A).
- the residue resulting from the treatment process of already degummed oil known industrially as lysogum, is applied in a pre-treatment of already degummed oils for subsequent treatment and transesterification of biodiesel.
- lysogum is presented as a pre-treatment, as it aims to reduce the levels of phosphorus, calcium and magnesium present in the degummed oil, through the action of active enzymes present in lysogum and through intermolecular interactions.
- Phospholipases are enzymes that cleave the fatty acid at position two of phospholipids, hydrolyzing the bond between the fatty acid and the glycerol molecule. This particular phospholipase specifically recognizes the bond to be cleaved.
- the enzyme PLA Phospholipase A selectively hydrolyzes the fatty acid opposite the phosphate functional group in the glycerol structure, producing a lyso-phospholipid and a fatty acid.
- the lysophospholipid molecule that has lost a hydrophilic functional group and the remaining alcohol group at the reaction site is hydrophilic. From the two hydrophilic sites, the lyso-phospholipid molecule becomes water-soluble and loses its emulsifying properties. Therefore, the PLA (Phospholipase A) treatment process of the already degummed oil reduces refining losses.
- the reaction of the phospholipase enzyme with phospholipids can take many hours, depending on reaction variables such as pH, temperature, relative concentrations, and mixing conditions.
- PLA enzymatic treatment is typically performed on vegetable oils and compositions that have first been subjected to degumming to obtain a product that has a phosphorus level low enough for the intended purposes.
- the PLA enzymatic treatment in the already degummed oil is done in order to reduce the remaining levels of phosphorus, calcium and magnesium.
- Document BR102014021463 (Petrobras. 2014) describes a process for the treatment of acidic oils through an enzyme-catalyzed esterification reaction, which aims to make biodiesel production competitive, using low-cost raw materials and energy savings , reducing the operating cost of production.
- EP1999241 (Cargill. 2006) describes a system and method for degumming a vegetable oil comprising mixing a feed stream under ultra-high shear conditions to provide a mixed stream, passing the mixed stream through a tank trap and separate the mixed stream into an aqueous stream and an oil stream.
- the object of the present invention is a process for the reuse of lysogum applied in the pre-treatment of vegetable oils for subsequent PLA enzymatic treatment and transesterification of biodiesel.
- lysogum which contains active enzymes
- the use of lysogum, which contains active enzymes is presented as a pre-treatment as it aims to reduce the levels of phosphorus, calcium and magnesium present in the already degummed oil.
- This new process results in the reuse of a by-product with no commercial value that helps to significantly reduce the levels of phosphorus, calcium and magnesium, which negatively interfere with the modification processes of vegetable oils, especially in the stages of enzymatic treatment and transesterification for the production of biodiesel.
- a feature of the present invention is the process of reuse of lysogum from the enzymatic treatment step PLA (Phospholipase A) in oil already degummed. Since the lysogome contains active enzymes and the capacity for molecular interactions, its reuse aims to improve the quality of the oil for the other steps.
- PLA Phospholipase A
- Characteristic of the present invention is a process for the reuse of lysogum that provides a technological application of a waste with no commercial value, helping to increase the product yield and reduction of production costs as in the amount of enzyme in the enzymatic treatment and in the consumption of catalyst in the subsequent transesterification reaction of biodiesel.
- Figure 1 represents the lysogoma from the conventional enzymatic treatment PLA (Phospholipase A).
- Figure 2 shows stages of the conventional PLA process.
- Figure 3 shows the simplified scheme of the lysogum reuse process for a pre-treatment of already degummed oil, detailing the steps of PLA enzymatic processing and the new pre-treatment process of lysogum reuse.
- the process of reuse of lysogum applied in the pre-treatment of oils already degummed, object of the present invention comprises a stage of reuse of lysogum, which still contains active enzymes, derived from the enzymatic treatment PLA in the oil already degummed.
- the lysogum reuse process aims to reduce the levels of phosphorus, calcium and magnesium in the already degummed oil.
- the conventional PLA process (hydrolysis by the action of phospholipases A) occurs from the reaction step (11) obtained from the addition of the PLA enzyme and water (3.0 to 3.5%) proportional to the volume of already degummed oil that enters the process (80). After a certain reaction time (11), the enzyme/oil mixture goes to the centrifugation step (12), which aims to remove undesirable constituents.
- the residue generated in this conventional process is called Lysogum, which consists of water, phosphorus, calcium, magnesium and phospholipase enzymes. active.
- Figure 2 shows the stages of the conventional PLA process.
- the lysogum reuse process has the following steps: a) The lysogum (10), resulting from the enzymatic treatment PLA (11) (hydrolysis by the action of phospholipases) of the degummed oil is returned in continuous flow to a mixer (20 ) and reactor (30) for the pre-treatment of the degummed oil (40), that is, the lysogum (10) generated in the original PLA process is redirected to a new stage (100) to be used in the pre-treatment of the degummed oil; b) The degummed oil (40) is heated through a heat exchanger (50), between 50-70°C, for subsequent mixing (20) with the lysogum (100).
- the temperature conditions may vary according to the enzyme used in the PLA enzymatic process (11).
- the oil-lysogum mixture proceeds to the reaction stage, which takes place in a reactor (30). In this step, hydrolysis of phospholipid bonds occurs. Also by molecular interaction and drag force there is a decrease in phosphorus, calcium and magnesium contents.
- the lysogum (100) can be separated from the oil by a decantation (60) or centrifugation (70) operation to remove it from the processing line.
- the degummed oil passes through the new pre-treatment, it is placed in a tank (80) where it undergoes the conventional enzymatic treatment PLA (11) and centrifugation (12) for the formation of lysogum (10) following the continuous flow of lysogome formation.
- the lysogum (100) is then removed from the oil, because it contains phosphorus, calcium and magnesium, in addition to inactive enzymes that harm the other stages (13) of oil treatment and transesterification; e)
- the oil with low levels of phosphorus, calcium and magnesium goes to the conventional PLA treatment and later to the biodiesel transesterification stage. It is noteworthy that the steps may vary, depending on the processing plant, the direction or processing of these oils.
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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PCT/BR2021/050555 WO2023108233A1 (pt) | 2021-12-16 | 2021-12-16 | Processo de reaproveitamento da lisogoma aplicado no pré-tratamento de óleos vegetais degomados para posterior tratamento enzimático e transesterificação do biodiesel |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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PCT/BR2021/050555 WO2023108233A1 (pt) | 2021-12-16 | 2021-12-16 | Processo de reaproveitamento da lisogoma aplicado no pré-tratamento de óleos vegetais degomados para posterior tratamento enzimático e transesterificação do biodiesel |
Publications (1)
Publication Number | Publication Date |
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WO2023108233A1 true WO2023108233A1 (pt) | 2023-06-22 |
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Family Applications (1)
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PCT/BR2021/050555 WO2023108233A1 (pt) | 2021-12-16 | 2021-12-16 | Processo de reaproveitamento da lisogoma aplicado no pré-tratamento de óleos vegetais degomados para posterior tratamento enzimático e transesterificação do biodiesel |
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WO (1) | WO2023108233A1 (pt) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5264367A (en) * | 1991-05-16 | 1993-11-23 | Rohm Gmbh | Enzymatic treatment of edible oils |
US5558781A (en) * | 1993-11-19 | 1996-09-24 | Metallgesellschaft Aktiengesellschaft | Process for enzymatically degumming vegetable oil |
CA2227568C (en) * | 1995-07-26 | 2006-05-30 | Rohm Gmbh | Enzymatic process for degumming plant oils using aspergillus phospholipase |
WO2013160374A1 (en) * | 2012-04-27 | 2013-10-31 | Dupont Nutrition Biosciences Aps | Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling |
-
2021
- 2021-12-16 WO PCT/BR2021/050555 patent/WO2023108233A1/pt active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5264367A (en) * | 1991-05-16 | 1993-11-23 | Rohm Gmbh | Enzymatic treatment of edible oils |
US5558781A (en) * | 1993-11-19 | 1996-09-24 | Metallgesellschaft Aktiengesellschaft | Process for enzymatically degumming vegetable oil |
CA2227568C (en) * | 1995-07-26 | 2006-05-30 | Rohm Gmbh | Enzymatic process for degumming plant oils using aspergillus phospholipase |
WO2013160374A1 (en) * | 2012-04-27 | 2013-10-31 | Dupont Nutrition Biosciences Aps | Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling |
Non-Patent Citations (4)
Title |
---|
ALBERT J. DIJKSTRA: "Edible Oil Processing from a Patent Perspective", 30 November 2012, SPRINGER NEW YORK, NY, ISBN: 978-1-4614-3350-7, article ALBERT J. DIJKSTRA: "Degumming", pages: 121 - 155, XP009547461, DOI: 10.1007/978-1-4614-3351-4_5 * |
BARBOSA JONATAS DE SANTANA, SAMPAIO KLICIA ARAUJO: "Efeito do processo de degomagem enzimática no rendimento e na qualidade do óleo de soja", REVISTA DOS TRABALHOS DE INICIAÇÃO CIENTÍFICA DA UNICAMP, no. 26, 1 January 2018 (2018-01-01), pages 1 - 1, XP093075969, DOI: 10.20396/revpibic262018690 * |
KIM CLAUSEN: "Enzymatic oil‐degumming by a novel microbial phospholipase", EUROPEAN JOURNAL OF LIPID SCIENCE TECHNOLOGY, vol. 103, no. 6, 18 June 2001 (2001-06-18), DE , pages 333 - 340, XP072142470, ISSN: 1438-7697, DOI: 10.1002/1438-9312(200106)103:6<333::AID-EJLT333>3.0.CO;2-F * |
KLICIA A. SAMPAIO; NADEZHDA ZYAYKINA; BOGUMILA WOZNIAK; JUNKO TSUKAMOTO; WIM DE GREYT; CHRISTIAN V. STEVENS: "Enzymatic degumming: Degumming efficiency versus yield increase", EUROPEAN JOURNAL OF LIPID SCIENCE TECHNOLOGY, vol. 117, no. 1, 22 August 2014 (2014-08-22), DE , pages 81 - 86, XP072144407, ISSN: 1438-7697, DOI: 10.1002/ejlt.201400218 * |
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