ES2176177T3 - Procedimiento para aumentar la acumulacion de esteroles en s. cerevisiae por transformacion de un mutante. - Google Patents
Procedimiento para aumentar la acumulacion de esteroles en s. cerevisiae por transformacion de un mutante.Info
- Publication number
- ES2176177T3 ES2176177T3 ES91310494T ES91310494T ES2176177T3 ES 2176177 T3 ES2176177 T3 ES 2176177T3 ES 91310494 T ES91310494 T ES 91310494T ES 91310494 T ES91310494 T ES 91310494T ES 2176177 T3 ES2176177 T3 ES 2176177T3
- Authority
- ES
- Spain
- Prior art keywords
- dna
- expression
- activity
- accumulation
- transformation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
SE DESCRIBE UN METODO PARA INCREMENTAR LA ACUMULACION DE ESQUALENO Y ESTEROLES ESPECIFICOS EN UNA LEVADURA, QUE COMPRENDE INCREMENTAR EL NIVEL DE EXPRESION DEL CODIGO DE UN GENE ESTRUCTURAL DE UN POLIPEPTIDO QUE TIENE LA ACTIVIDAD DE LA REDUCTASA HMG-COA EN UNA LEVADURA MUTANTE QUE TIENE EFECTOS UNICOS O DOBLES EN LA EXPRESION DE LAS BISINTETICENZIMAS ESTEROL. EL NIVEL DE EXPRESION DEL GENE ESTRUCTURAL INCREMENTADO PREFERENTEMENTE MEDIANTE LA TRANSFORMACION DE LA LEVADURA CON UNA MOLECULA RECOMBINANTE DE DNA QUE COMPRENDE UN VECTOR OPERATIVAMENTE LIGADO A UN SEGMENTO EXOGENO DE DNA QUE CODIFICA UN POLIPEPTIDO QUE TIENE ACTIVIDAD DE LA REDUCTASA HMG-COA Y UN PROMOTOR QUE ES UTIL PARA CONDUCIR LA EXPRESION DEL PEPTIDO MODIFICADO DE LA LEVADURA TRANSFORMADA. EL POLIPEPTIDO CON ACTIVIDAD DE LA REDUCTASA HMG-COA ES PREFERENTEMENTE UNA ENZIMA REDUCTASA HMG-COA ACTIVA TRUNCADA. TAMBIEN SE DESCRIBEN MOLECULAS RECOMBINANTES DEL DNA UTILES PARA TRANSFORMAR LEVADURAS Y LEVADURAS MUTANTES TRANSFORMADASCON DICHA RECOMBINACION DE MOLECULAS DE DNA.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US61338090A | 1990-11-15 | 1990-11-15 | |
US07/783,861 US5460949A (en) | 1990-11-15 | 1991-10-28 | Method and composition for increasing the accumulation of squalene and specific sterols in yeast |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2176177T3 true ES2176177T3 (es) | 2002-12-01 |
Family
ID=27087007
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES91310494T Expired - Lifetime ES2176177T3 (es) | 1990-11-15 | 1991-11-14 | Procedimiento para aumentar la acumulacion de esteroles en s. cerevisiae por transformacion de un mutante. |
Country Status (6)
Country | Link |
---|---|
US (1) | US5460949A (es) |
EP (1) | EP0486290B1 (es) |
JP (1) | JP3283551B2 (es) |
DE (1) | DE69133012T2 (es) |
DK (1) | DK0486290T3 (es) |
ES (1) | ES2176177T3 (es) |
Families Citing this family (55)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5741898A (en) * | 1994-09-22 | 1998-04-21 | Hanley; Kathleen Marie | DNA sequence encoding nicotiana squalene synthetase |
US20020086380A1 (en) * | 1996-03-29 | 2002-07-04 | Francis X. Cunningham Jr | Genes encoding epsilon lycopene cyclase and method for producing bicyclic carotene |
DE19744212B4 (de) * | 1997-09-30 | 2006-01-19 | Schering Ag | Verfahren zur Herstellung von Ergosterol und dessen Zwischenprodukten mittels rekombinanter Hefen |
TWI250210B (en) | 1998-05-06 | 2006-03-01 | Dsm Ip Assets Bv | An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate |
EP0955363A3 (en) * | 1998-05-06 | 2004-01-28 | F. Hoffmann-La Roche Ag | Dna sequences encoding enzymes involved in production of isoprenoids |
BR9911873A (pt) | 1998-07-06 | 2001-03-27 | Eastman Chem Co | Processo para produzir fitol, um derivado de fiteno, isofitol, um derivado de isofiteno, ou uma mistura dos mesmos, composto de epoxifitila substituìdo, composto de fiteno substituìdo, processo para produzir o mesmo, processo para produzir alfa-tocoferol, e, processo para coverter geranilgeraniol a alfa-tocoferol |
US6531303B1 (en) | 1998-07-06 | 2003-03-11 | Arkion Life Sciences Llc | Method of producing geranylgeraniol |
US6410755B1 (en) | 1998-07-06 | 2002-06-25 | Dcv, Inc. | Method of vitamin production |
DK2100963T3 (da) * | 1998-07-06 | 2012-04-02 | Dcv Inc | Fremgangsmåde til vitaminfremstilling. |
US6399796B2 (en) | 2000-03-17 | 2002-06-04 | Roche Vitamins Inc. | Activation of a Diels-Alder reaction of a sterol 5,7-diene |
US7009045B2 (en) * | 2000-07-14 | 2006-03-07 | Archer-Daniels-Midland Company | Transformation systems for flavinogenic yeast |
CA2433529A1 (en) * | 2000-12-28 | 2002-07-11 | Toyota Jidosha Kabushiki Kaisha | Process for producing prenyl alcohol |
JP3838033B2 (ja) * | 2000-12-28 | 2006-10-25 | トヨタ自動車株式会社 | プレニルアルコールの製造方法 |
EP1354956A4 (en) * | 2000-12-28 | 2004-10-27 | Toyota Motor Co Ltd | PROCESS FOR PRODUCING PRENYL ALCOHOL |
US6822142B2 (en) * | 2001-01-05 | 2004-11-23 | Monsanto Company | Transgenic plants containing altered levels of steroid compounds |
DE10203352A1 (de) * | 2002-01-29 | 2003-07-31 | Basf Ag | Verfahren zur Herstellung von 7-Dehydrocholesterol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen |
DE10312314A1 (de) | 2003-03-19 | 2004-09-30 | Basf Ag | Verfahren zur Herstellung von Ergosta-5,7-dienol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen |
FR2869914B1 (fr) * | 2004-05-06 | 2012-11-09 | Aventis Pharma Sa | Souches de levure produisant du cholesterol et leurs applications |
ATE537269T1 (de) * | 2004-05-21 | 2011-12-15 | Univ California | Verfahren zur verbesserung der herstellung von isoprenoid-zusammensetzungen |
CA2573613A1 (en) * | 2004-07-13 | 2006-01-19 | Mitsubishi Chemical Corporation | A method for producing a steroid compound |
JP5590769B2 (ja) | 2004-07-27 | 2014-09-17 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | イソプレノイド化合物を生産するための遺伝的に修飾された宿主細胞および同宿主細胞の使用方法 |
EP2371967B1 (en) | 2005-03-18 | 2015-06-03 | DSM IP Assets B.V. | Production of carotenoids in oleaginous yeast and fungi |
WO2007024718A2 (en) * | 2005-08-19 | 2007-03-01 | The Regents Of The University Of California | Genetically modified host cells and use of same for producing isoprenoid compounds |
JP2009511020A (ja) * | 2005-10-07 | 2009-03-19 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 改変チトクロームp450酵素をコードする核酸およびその使用法 |
JP2007210888A (ja) | 2006-01-12 | 2007-08-23 | Mitsubishi Chemicals Corp | ステロイド化合物の製造方法 |
US8114645B2 (en) * | 2006-05-19 | 2012-02-14 | The Regents Of The University Of California | Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels |
KR20120053088A (ko) | 2006-05-26 | 2012-05-24 | 아미리스 인코퍼레이티드 | 이소프레노이드의 생산 방법 |
EP2078092A2 (en) | 2006-09-28 | 2009-07-15 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
EP2074214A2 (en) | 2006-09-28 | 2009-07-01 | Microbia, Inc. | Production of sterols in oleaginous yeast and fungi |
WO2009006429A1 (en) * | 2007-06-29 | 2009-01-08 | Regents Of The University Of California | Host cells and methods for producing 3-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, and 3-methyl-butan-1-ol |
WO2009006386A2 (en) | 2007-06-29 | 2009-01-08 | The Regents Of The University Of California | Host cells and methods for producing isoprenyl alkanoates |
JP2011520471A (ja) | 2008-05-23 | 2011-07-21 | サイバス オイルズ,エルエルシー | 酵母を使用したスクアレンの生成 |
EP2369003B1 (en) * | 2008-08-28 | 2019-10-16 | Novartis AG | Process for preparing an oil-in-water emulsion containing squalene from yeasts |
DE102009022772A1 (de) * | 2009-05-21 | 2010-11-25 | Organobalance Gmbh | Mikroorganismus zur Expression eines humanen Membranproteins |
JP4821886B2 (ja) * | 2009-06-04 | 2011-11-24 | トヨタ自動車株式会社 | 組換え酵母、当該組換え酵母を用いた分岐アルコールの製造方法 |
AU2010266147A1 (en) * | 2009-07-01 | 2012-01-19 | The Regents Of The University Of California | Extraction of extracellular terpenoids from microalgae colonies |
EP2292741A1 (en) | 2009-08-26 | 2011-03-09 | OrganoBalance GmbH | Genetically modified organisms for the production of lipids |
EA201290199A1 (ru) | 2009-11-23 | 2014-05-30 | Нукелис Инк. | Способы и композиции для получения сквалена с применением дрожжей |
US9624521B2 (en) | 2009-12-03 | 2017-04-18 | Dsm Ip Assets B.V. | Production of non-yeast sterols by yeast |
MY180803A (en) | 2010-06-02 | 2020-12-09 | Evolva Inc | Recombinant production of steviol glycosides |
KR101944841B1 (ko) * | 2011-02-28 | 2019-02-01 | 노보자임스 에이/에스 | 테르펜을 생산하기 위한 효모 세포와 이의 용도 |
KR101983115B1 (ko) | 2011-08-08 | 2019-05-29 | 에볼바 에스아 | 사프란 화합물의 재조합 생성을 위한 방법 및 물질 |
WO2013022989A2 (en) | 2011-08-08 | 2013-02-14 | Evolva Sa | Recombinant production of steviol glycosides |
AU2014214004B2 (en) | 2013-02-06 | 2018-05-24 | Evolva Sa | Methods for improved production of Rebaudioside D and Rebaudioside M |
MY184253A (en) | 2013-02-11 | 2021-03-29 | Evolva Sa | Efficient production of steviol glycosides in recombinant hosts |
SG11201506290RA (en) * | 2013-03-13 | 2015-09-29 | Sanofi Aventis Deutschland | Production of squalene and/or sterol from cell suspensions of fermented yeast |
AU2015244729C1 (en) | 2014-04-09 | 2021-05-27 | Adeka Corporation | Mutant enzyme and production method for terpenoid using said mutant enzyme |
SG10201901007PA (en) | 2014-08-11 | 2019-03-28 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
AU2015314251A1 (en) | 2014-09-09 | 2017-03-16 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
US10364450B2 (en) | 2015-01-30 | 2019-07-30 | Evolva Sa | Production of steviol glycoside in recombinant hosts |
US10604743B2 (en) | 2015-03-16 | 2020-03-31 | Dsm Ip Assets B.V. | UDP-glycosyltransferases |
EP3332018B1 (en) | 2015-08-07 | 2022-07-27 | Evolva SA | Production of steviol glycosides in recombinant hosts |
MY198001A (en) | 2016-04-13 | 2023-07-25 | Evolva Sa | Production of steviol glycoside in recombinant hosts |
WO2017198682A1 (en) | 2016-05-16 | 2017-11-23 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
WO2018083338A1 (en) | 2016-11-07 | 2018-05-11 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
-
1991
- 1991-10-28 US US07/783,861 patent/US5460949A/en not_active Expired - Lifetime
- 1991-11-14 DK DK91310494T patent/DK0486290T3/da active
- 1991-11-14 ES ES91310494T patent/ES2176177T3/es not_active Expired - Lifetime
- 1991-11-14 DE DE69133012T patent/DE69133012T2/de not_active Expired - Lifetime
- 1991-11-14 EP EP91310494A patent/EP0486290B1/en not_active Expired - Lifetime
- 1991-11-15 JP JP30076991A patent/JP3283551B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69133012T2 (de) | 2003-01-02 |
JP3283551B2 (ja) | 2002-05-20 |
DE69133012D1 (de) | 2002-06-27 |
EP0486290B1 (en) | 2002-05-22 |
JPH05192184A (ja) | 1993-08-03 |
US5460949A (en) | 1995-10-24 |
EP0486290A3 (en) | 1993-03-17 |
DK0486290T3 (da) | 2002-09-16 |
EP0486290A2 (en) | 1992-05-20 |
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