ES2176177T3 - Procedimiento para aumentar la acumulacion de esteroles en s. cerevisiae por transformacion de un mutante. - Google Patents

Procedimiento para aumentar la acumulacion de esteroles en s. cerevisiae por transformacion de un mutante.

Info

Publication number
ES2176177T3
ES2176177T3 ES91310494T ES91310494T ES2176177T3 ES 2176177 T3 ES2176177 T3 ES 2176177T3 ES 91310494 T ES91310494 T ES 91310494T ES 91310494 T ES91310494 T ES 91310494T ES 2176177 T3 ES2176177 T3 ES 2176177T3
Authority
ES
Spain
Prior art keywords
dna
expression
activity
accumulation
transformation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91310494T
Other languages
English (en)
Inventor
Court A Saunders
Fred Richard Wolf
Indrani Mukharji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP Corp North America Inc
Original Assignee
BP Corp North America Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BP Corp North America Inc filed Critical BP Corp North America Inc
Application granted granted Critical
Publication of ES2176177T3 publication Critical patent/ES2176177T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE DESCRIBE UN METODO PARA INCREMENTAR LA ACUMULACION DE ESQUALENO Y ESTEROLES ESPECIFICOS EN UNA LEVADURA, QUE COMPRENDE INCREMENTAR EL NIVEL DE EXPRESION DEL CODIGO DE UN GENE ESTRUCTURAL DE UN POLIPEPTIDO QUE TIENE LA ACTIVIDAD DE LA REDUCTASA HMG-COA EN UNA LEVADURA MUTANTE QUE TIENE EFECTOS UNICOS O DOBLES EN LA EXPRESION DE LAS BISINTETICENZIMAS ESTEROL. EL NIVEL DE EXPRESION DEL GENE ESTRUCTURAL INCREMENTADO PREFERENTEMENTE MEDIANTE LA TRANSFORMACION DE LA LEVADURA CON UNA MOLECULA RECOMBINANTE DE DNA QUE COMPRENDE UN VECTOR OPERATIVAMENTE LIGADO A UN SEGMENTO EXOGENO DE DNA QUE CODIFICA UN POLIPEPTIDO QUE TIENE ACTIVIDAD DE LA REDUCTASA HMG-COA Y UN PROMOTOR QUE ES UTIL PARA CONDUCIR LA EXPRESION DEL PEPTIDO MODIFICADO DE LA LEVADURA TRANSFORMADA. EL POLIPEPTIDO CON ACTIVIDAD DE LA REDUCTASA HMG-COA ES PREFERENTEMENTE UNA ENZIMA REDUCTASA HMG-COA ACTIVA TRUNCADA. TAMBIEN SE DESCRIBEN MOLECULAS RECOMBINANTES DEL DNA UTILES PARA TRANSFORMAR LEVADURAS Y LEVADURAS MUTANTES TRANSFORMADASCON DICHA RECOMBINACION DE MOLECULAS DE DNA.
ES91310494T 1990-11-15 1991-11-14 Procedimiento para aumentar la acumulacion de esteroles en s. cerevisiae por transformacion de un mutante. Expired - Lifetime ES2176177T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61338090A 1990-11-15 1990-11-15
US07/783,861 US5460949A (en) 1990-11-15 1991-10-28 Method and composition for increasing the accumulation of squalene and specific sterols in yeast

Publications (1)

Publication Number Publication Date
ES2176177T3 true ES2176177T3 (es) 2002-12-01

Family

ID=27087007

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91310494T Expired - Lifetime ES2176177T3 (es) 1990-11-15 1991-11-14 Procedimiento para aumentar la acumulacion de esteroles en s. cerevisiae por transformacion de un mutante.

Country Status (6)

Country Link
US (1) US5460949A (es)
EP (1) EP0486290B1 (es)
JP (1) JP3283551B2 (es)
DE (1) DE69133012T2 (es)
DK (1) DK0486290T3 (es)
ES (1) ES2176177T3 (es)

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* Cited by examiner, † Cited by third party
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US5741898A (en) * 1994-09-22 1998-04-21 Hanley; Kathleen Marie DNA sequence encoding nicotiana squalene synthetase
US20020086380A1 (en) * 1996-03-29 2002-07-04 Francis X. Cunningham Jr Genes encoding epsilon lycopene cyclase and method for producing bicyclic carotene
DE19744212B4 (de) * 1997-09-30 2006-01-19 Schering Ag Verfahren zur Herstellung von Ergosterol und dessen Zwischenprodukten mittels rekombinanter Hefen
EP0955363A3 (en) * 1998-05-06 2004-01-28 F. Hoffmann-La Roche Ag Dna sequences encoding enzymes involved in production of isoprenoids
TWI250210B (en) 1998-05-06 2006-03-01 Dsm Ip Assets Bv An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate
DK2305825T3 (en) 1998-07-06 2015-03-02 Dcv Inc A process for the production of vitamins
US6410755B1 (en) 1998-07-06 2002-06-25 Dcv, Inc. Method of vitamin production
US6531303B1 (en) 1998-07-06 2003-03-11 Arkion Life Sciences Llc Method of producing geranylgeraniol
JP2002519416A (ja) 1998-07-06 2002-07-02 イーストマン ケミカル カンパニー トコフェロールの製造
US6399796B2 (en) 2000-03-17 2002-06-04 Roche Vitamins Inc. Activation of a Diels-Alder reaction of a sterol 5,7-diene
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
EP1354956A4 (en) * 2000-12-28 2004-10-27 Toyota Motor Co Ltd PROCESS FOR PRODUCING PRENYL ALCOHOL
JP3838033B2 (ja) * 2000-12-28 2006-10-25 トヨタ自動車株式会社 プレニルアルコールの製造方法
EP1354955A4 (en) * 2000-12-28 2005-01-05 Toyota Motor Co Ltd PROCESS FOR PREPARING PRENYL ALCOHOL
US6822142B2 (en) * 2001-01-05 2004-11-23 Monsanto Company Transgenic plants containing altered levels of steroid compounds
DE10203352A1 (de) * 2002-01-29 2003-07-31 Basf Ag Verfahren zur Herstellung von 7-Dehydrocholesterol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
DE10312314A1 (de) * 2003-03-19 2004-09-30 Basf Ag Verfahren zur Herstellung von Ergosta-5,7-dienol und/oder dessen biosynthetischen Zwischen- und/oder Folgeprodukten in transgenen Organismen
FR2869914B1 (fr) * 2004-05-06 2012-11-09 Aventis Pharma Sa Souches de levure produisant du cholesterol et leurs applications
US7183089B2 (en) 2004-05-21 2007-02-27 The Regents Of The University Of California Method for enhancing production of isoprenoid compounds
EP1775304A4 (en) * 2004-07-13 2010-04-28 Mitsubishi Chem Corp PROCESS FOR THE PRODUCTION OF STEROIDS
EP1778831B1 (en) 2004-07-27 2012-05-23 The Regents of The University of California Genetically modified host cells and use of same for producing isoprenoid compounds
KR101482081B1 (ko) 2005-03-18 2015-01-13 마이크로비아 인크. 유질 효모와 진균 내에서 카로티노이드의 생산
US20090053797A1 (en) * 2005-08-19 2009-02-26 Yoichiro Shiba Genetically modified host cells and use of same for producing isoprenoid compounds
EP1931770A4 (en) * 2005-10-07 2009-11-25 Univ California NUCLEIC ACIDS CODING FOR MODIFIED CYTOCHROM P450 ENZYME AND METHOD OF USE THEREOF
JP2007210888A (ja) 2006-01-12 2007-08-23 Mitsubishi Chemicals Corp ステロイド化合物の製造方法
WO2007136847A2 (en) * 2006-05-19 2007-11-29 The Regents Of The University Of California Methods for increasing isoprenoid and isoprenoid precursor production by modulating fatty acid levels
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US8367395B2 (en) 2006-09-28 2013-02-05 Dsm Ip Assets B.V. Production of sterols in oleaginous yeast and fungi
WO2009006386A2 (en) 2007-06-29 2009-01-08 The Regents Of The University Of California Host cells and methods for producing isoprenyl alkanoates
WO2009006429A1 (en) * 2007-06-29 2009-01-08 Regents Of The University Of California Host cells and methods for producing 3-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, and 3-methyl-butan-1-ol
JP2011520471A (ja) * 2008-05-23 2011-07-21 サイバス オイルズ，エルエルシー 酵母を使用したスクアレンの生成
PT2268823E (pt) * 2008-08-28 2011-09-01 Novartis Ag Produção de esqualeno a partir de leveduras hiperprodutoras
DE102009022772A1 (de) 2009-05-21 2010-11-25 Organobalance Gmbh Mikroorganismus zur Expression eines humanen Membranproteins
JP4821886B2 (ja) * 2009-06-04 2011-11-24 トヨタ自動車株式会社 組換え酵母、当該組換え酵母を用いた分岐アルコールの製造方法
AU2010266147A1 (en) * 2009-07-01 2012-01-19 The Regents Of The University Of California Extraction of extracellular terpenoids from microalgae colonies
EP2292741A1 (en) * 2009-08-26 2011-03-09 OrganoBalance GmbH Genetically modified organisms for the production of lipids
ES2625154T3 (es) * 2009-11-23 2017-07-18 Nucelis Llc Métodos y composiciones para producir escualeno empleando levaduras
JP5889200B2 (ja) 2009-12-03 2016-03-22 ディーエスエム アイピー アセッツ ビー．ブイ． 酵母による非酵母ステロールの生成
BR112012030836B8 (pt) 2010-06-02 2024-02-06 Evolva Nutrition Inc Hospedeiro recombinante que compreende genes recombinantes para produção de esteviol ou glicosídeo de esteviol, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol
KR101944841B1 (ko) * 2011-02-28 2019-02-01 노보자임스 에이/에스 테르펜을 생산하기 위한 효모 세포와 이의 용도
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SG10201705993YA (en) 2013-02-11 2017-08-30 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
CN105431542B (zh) * 2013-03-13 2020-04-07 赛诺菲-安万特德国有限公司 从发酵的酵母的细胞悬浮液中生产角鲨烯和/或甾醇
EP3130672A4 (en) * 2014-04-09 2017-12-20 Adeka Corporation Mutant enzyme and production method for terpenoid using said mutant enzyme
BR112017002783A2 (pt) 2014-08-11 2017-12-19 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
MX2017003130A (es) 2014-09-09 2017-10-24 Evolva Sa Produccion de glucosidos de esteviol en hospederos recombinantes.
CA2973674A1 (en) 2015-01-30 2016-08-04 Evolva Sa Production of steviol glycosides in recombinant hosts
CN115851470A (zh) 2015-03-16 2023-03-28 帝斯曼知识产权资产管理有限公司 Udp-糖基转移酶
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109312378A (zh) 2016-05-16 2019-02-05 埃沃尔瓦公司 在重组宿主中产生甜菊醇糖苷
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts

Also Published As

Publication number Publication date
DE69133012D1 (de) 2002-06-27
EP0486290A3 (en) 1993-03-17
DK0486290T3 (da) 2002-09-16
US5460949A (en) 1995-10-24
JP3283551B2 (ja) 2002-05-20
EP0486290B1 (en) 2002-05-22
EP0486290A2 (en) 1992-05-20
DE69133012T2 (de) 2003-01-02
JPH05192184A (ja) 1993-08-03

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